专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09152150B1 Compositions, systems, and methods for immiscible fluid discrete volume manipulation 有权
标题翻译：用于不混溶流体离散体积操纵的组合物，系统和方法
公开(公告)号：US09152150B1
公开(公告)日：2015-10-06
申请号：US12036037
申请日：2008-02-22
申请人： Linda G. Lee , Mark F. Oldham , Sam L. Woo , David M. Cox , Richard T. Reel , Peter N. Ma , Ben F. Johnson , Dennis Letho
发明人： Linda G. Lee , Mark F. Oldham , Sam L. Woo , David M. Cox , Richard T. Reel , Peter N. Ma , Ben F. Johnson , Dennis Letho
IPC分类号： G05D11/00 , G05D7/06 , B01L3/00 , B01F13/00 , G01N35/08 , B01L3/02
CPC分类号： B01F3/0811 , B01F3/0815 , B01F5/0471 , B01F13/0071 , B01F13/0072 , B01F13/0076 , B01F2003/0842 , B01L3/0265 , B01L3/502769 , B01L3/502784 , B01L2200/06 , B01L2200/0605 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , B01L2400/0666 , G01N1/38 , G01N21/6428 , G01N35/08 , G01N2001/386 , G01N2021/6439 , G05D7/0694 , G05D11/131
摘要： Systems and methods of manipulating discrete volumes of a first fluid in a second fluid are provided. In some embodiments, discrete volumes can be formed in a conduit. In other embodiments, addition fluid can be added to a discrete volume in a first conduit by injecting the addition fluid at a relatively higher pressure. In some embodiments, discrete volumes that normally would not coalesce can be manipulated to be merged together.
摘要翻译：提供了在第二流体中操纵第一流体的离散体积的系统和方法。在一些实施例中，离散体积可以形成在导管中。在其它实施方案中，可以通过在相对较高的压力下注入添加流体将添加流体添加到第一导管中的离散体积。在一些实施例中，通常不会聚结的离散体积可以被操纵以被合并在一起。

2. 发明授权

US07955864B2 Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other 有权
标题翻译：使与第二流体接触的第一流体的离散体积彼此不混溶的装置和方法
公开(公告)号：US07955864B2
公开(公告)日：2011-06-07
申请号：US11508044
申请日：2006-08-22
申请人： David M. Cox , Willy Wiyatno , Mark F. Oldham , James C. Nurse , Douglas P. Greiner , Sam L. Woo , Richard T. Reel , Dennis A. Lehto , Linda G. Lee , Janusz B. Wojtowicz
发明人： David M. Cox , Willy Wiyatno , Mark F. Oldham , James C. Nurse , Douglas P. Greiner , Sam L. Woo , Richard T. Reel , Dennis A. Lehto , Linda G. Lee , Janusz B. Wojtowicz
IPC分类号： G01N1/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
摘要翻译：在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。该系统可以包括一种装置，该装置包括至少一个导管，其操作地连接到一个或多个泵，用于提供通过与离散体积的流体不混溶的流体彼此分离的离散体积，例如水不混溶流体 - 油分离体积。

3. 发明授权

US06649598B2 4,7-dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US06649598B2
公开(公告)日：2003-11-18
申请号：US09949444
申请日：2001-09-07
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
IPC分类号： A01N4304
CPC分类号： C07H21/00 , A61K48/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777 , C12Q2563/107
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料，用于检测特别重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

4. 发明授权

US06403812B1 4, 7-Dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4，7-二氯荧光素染料作为分子探针
公开(公告)号：US06403812B1
公开(公告)日：2002-06-11
申请号：US09580754
申请日：2000-05-30
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
IPC分类号： C07D31182
CPC分类号： C07H21/00 , A61K48/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777 , C12Q2563/107
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

5. 发明授权

US5654442A 4,7-dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US5654442A
公开(公告)日：1997-08-05
申请号：US400780
申请日：1995-03-08
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68
CPC分类号： C07H21/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , A61K48/00 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

6. 发明授权

US6096723A 4,7-dichlorofluorescein dyes as molecular probes 有权
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US6096723A
公开(公告)日：2000-08-01
申请号：US273655
申请日：1999-03-23
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68 , A01N43/04
CPC分类号： C07H21/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , A61K48/00 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5', 7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

7. 发明授权

US5885778A 4, 7-dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US5885778A
公开(公告)日：1999-03-23
申请号：US905855
申请日：1997-08-04
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam L. Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68 , C07D311/78 , C07D311/94
CPC分类号： C07H21/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , A61K48/00 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

8. 发明授权

US5453505A N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels 失效
标题翻译： N-杂芳族离子和亚胺离子取代的花青染料用作荧光标记
公开(公告)号：US5453505A
公开(公告)日：1995-09-26
申请号：US268852
申请日：1994-06-30
申请人： Linda G. Lee , Sam L. Woo
发明人： Linda G. Lee , Sam L. Woo
IPC分类号： A61K49/00 , C09B23/01 , C09B23/02 , G01N33/533 , C07D209/02 , A61K47/22 , C07D209/56
CPC分类号： A61K49/0032 , C09B23/0041 , C09B23/0066 , C09B23/02 , G01N33/533 , Y10S435/968 , Y10S436/80 , Y10S530/802
摘要： The present invention relates to iminium ion substituted cyanine dyes having a fluoresence absorbance of between about 500 and 900 nm, a reduced tendency to aggregate and enhanced photostability. The cyanine dyes of the present invention are represented by the formula ##STR1## wherein n is 0, 1, 2 or 3;m is 0, 1, 2 or 3;R.sub.1 and R.sub.2 are taken together to form an aromatic ring or a fused polycyclic aromatic ring;R.sub.3 and R.sub.4 are taken together to form an aromatic ring or a fused polycyclic aromatic ring;R.sub.5 and R.sub.6 are independently selected from the group consisting of (CH.sub.2).sub.p X where p is 1-18 and X is a functional group that reacts with amino, hydroxyl and sulfhydryl nucleophiles;R.sub.7 and R.sub.8 are independently selected from the group consisting of hydrogen, C1-C10 alkyl groups and where R.sub.7 and R.sub.8 are taken together to form a five- or six- membered heterocyclic ring;R.sub.9 are each independently selected from the group consisting of hydrogen, alkyl and where more than one R.sub.9 are taken together to form a five- or six- membered ring;Y is selected from the group consisting of C(CH.sub.3).sub.2, S, O and Se; andZ is selected from the group consisting of C(CH.sub.3).sub.2, S, O and Se.
摘要翻译：本发明涉及具有约500至900nm之间的荧光吸收度的聚亚胺离子取代花青染料，具有降低的聚集倾向和增强的光稳定性。本发明的花青染料由下式表示：其中n为0,1,2或3; m为0,1,2或3; R1和R2一起形成芳环或稠合多环芳环; R3和R4一起形成芳环或稠合多环芳环; R5和R6独立地选自（CH2）pX，其中p是1-18，X是与氨基，羟基和巯基亲核试剂反应的官能团; R 7和R 8独立地选自氢，C 1 -C 10烷基，其中R 7和R 8一起形成五元或六元杂环; R 9各自独立地选自氢，烷基，并且其中多于一个的R 9一起形成五元或六元环; Y选自C（CH 3）2，S，O和Se; 并且Z选自C（CH 3）2，S，O和Se。

9. 发明授权

US5366860A Spectrally resolvable rhodamine dyes for nucleic acid sequence determination 失效
标题翻译：用于核酸序列测定的光谱可分辨的罗丹明染料
公开(公告)号：US5366860A
公开(公告)日：1994-11-22
申请号：US415050
申请日：1989-09-29
申请人： B. John Bergot , Vergine Chakerian , Charles R. Connell , J. Scott Eadie , Steven Fung , N. Davis Hershey , Linda G. Lee , Steven M. Menchen , Sam L. Woo
发明人： B. John Bergot , Vergine Chakerian , Charles R. Connell , J. Scott Eadie , Steven Fung , N. Davis Hershey , Linda G. Lee , Steven M. Menchen , Sam L. Woo
IPC分类号： C09B69/10 , C07H19/10 , C07H19/20 , C07H21/00 , C09B11/24 , C12Q1/68
CPC分类号： C12Q1/6869 , C07H21/00 , C09B11/24
摘要： A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
摘要翻译：提供光谱可分辨的罗丹明染料组合用于核酸测序的链终止方法。通过炔基氨基接头将与四氯乙酰胺，罗丹明X，罗丹明6G和罗丹明110组成的组中的不同的罗丹明染料连接到用于测序方法的每个双脱氧核苷酸的碱基上。优选地，标记的双脱氧核苷酸通过Taq DNA聚合酶掺入生长的DNA链中。

10. 发明授权

US5624800A Method of DNA sequencing employing a mixed DNA-polymer chain probe 失效
标题翻译：使用混合DNA-聚合物链探针进行DNA测序的方法
公开(公告)号：US5624800A
公开(公告)日：1997-04-29
申请号：US447174
申请日：1995-05-19
申请人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
发明人： Paul D. Grossman , Steven Fung , Steven M. Menchen , Sam L. Woo , Emily S. Winn-Deen
IPC分类号： C12N15/09 , B01D57/02 , C12Q1/68 , G01N27/447 , G01N30/88 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6818 , Y10T436/143333
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式