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    • 3. 发明授权
    • Acetolactate synthase gene promoter
    • 乙酰乳酸合酶基因启动子
    • US07238864B2
    • 2007-07-03
    • US11075808
    • 2005-03-10
    • Seiichi TokiHiroaki IchikawaHidemitsu NakamuraKiyoshi KawaiKoichiro KakuTsutomu Shimizu
    • Seiichi TokiHiroaki IchikawaHidemitsu NakamuraKiyoshi KawaiKoichiro KakuTsutomu Shimizu
    • C07H21/04C12N5/05C12N15/09A01H1/00A01H5/00
    • C12N15/8274C12N15/8222
    • The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.
    • 本发明提供诱导组织特异性表达的方法,其包括制备具有启动子的表达载体。 启动子包括以下DNA(a)至(c)中的任一种:(a)包含SEQ ID NO:1所示核苷酸序列的DNA; (b)DNA,其包含从SEQ ID NO:1的核苷酸1344至2843的核苷酸序列; 和(c)DNA,其是在严格条件下与包含与SEQ ID NO:1所示的核苷酸序列完全互补的核苷酸序列的DNA片段杂交的DNA片段,其中,(a),(b)或( c)可以控制位于其下游的编码序列的转录; 并且其中严格条件在42℃为5×SSC; 表达载体也包括位于启动子下游的编码序列。 此外,该方法包括将表达载体导入植物细胞以获得转基因植物; 其中成熟种子或根系中的启动子活性低于在成熟种子或根基以外的植物组织中的活性。 本发明还提供含有启动子的转化体; 启动子包括上述DNA(a)至(c)中的任一种。
    • 4. 发明授权
    • Silver halide color photographic light-sensitive material and method of
forming color image using the same
    • 卤化银彩色摄影感光材料及使用其形成彩色图像的方法
    • US5994046A
    • 1999-11-30
    • US873403
    • 1997-06-11
    • Kiyoshi Kawai
    • Kiyoshi Kawai
    • G03C1/83G03C7/30G03C7/38G03C7/388G03C1/08G03C7/26G03C7/32
    • G03C7/383G03C1/832G03C7/3022G03C7/3885
    • A silver halide color photographic light-sensitive material comprising a support having thereon photographic constituent layers comprising at least three silver halide emulsion layers each having a spectral sensitivity different from one another and containing yellow, magenta and cyan color forming couplers, respectively and a light-insensitive hydrophilic colloid layer, wherein the cyan color forming coupler is at least one dye forming coupler represented by the formula (I) shown below, at least one of the silver halide emulsion layers containing the cyan color forming coupler contains a silver halide emulsion composed of high silver chloride content grains having a silver chloride content of 80 mol % or more, and the high silver chloride content grains are tabular silver halide grains having (100) faces as major faces: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 each represents a hydrogen atom or a substituent; Z represents a non-metallic atomic group necessary for forming a ring, which may be substituted; X represents a heterocyclic group, a substituted amino group or an aryl group; Y represents a hydrogen atom or a substituent; and R.sub.6 represents a substituent.The silver halide color photographic light-sensitive material is rapidly processable, has less dependence on processing conditions and provides a color image having excellent image quality and sharpness. It is also suitable for scanning exposure using a semiconductor laser as a light source. A method of forming a color image comprising subjecting the silver halide color photographic light-sensitive material to scanning exposure is also disclosed.
    • 一种卤化银彩色摄影感光材料,其包含其上具有包含至少三个卤化银乳剂层的照相构成层的载体,每个卤化银乳剂层各自具有彼此不同的光谱灵敏度,并且分别含有黄色,品红色和青色形成成色剂, 不敏感的亲水胶体层,其中青色成色剂是至少一种由下述式(I)表示的染料形成成色剂,含有青色成色剂的卤化银乳剂层中的至少一种含有由 氯化银含量为80摩尔%以上的高氯化银含量的颗粒,高氯化银含量颗粒是具有(100)面作为主面的片状卤化银颗粒:其中R1,R2,R3,R4和R5各自表示 氢原子或取代基; Z表示可以被取代的形成环所必需的非金属原子团; X表示杂环基,取代氨基或芳基; Y表示氢原子或取代基; R6表示取代基。 卤化银彩色摄影感光材料可快速处理,对处理条件的依赖性较小,并提供具有优异图像质量和清晰度的彩色图像。 也适用于使用半导体激光器作为光源进行扫描曝光。 还公开了一种形成彩色图像的方法,包括使卤化银彩色照相感光材料经受扫描曝光。
    • 5. 发明授权
    • Method of forming colored images
    • 形成彩色图像的方法
    • US5747227A
    • 1998-05-05
    • US305298
    • 1994-09-15
    • Kiyoshi Kawai
    • Kiyoshi Kawai
    • G03C1/035G03C5/04G03C5/08G03C7/30G03C7/36G03C7/392G03C7/407
    • G03C7/30G03C7/36G03C5/04G03C7/3022G03C7/3041G03C7/39284G03C7/407
    • A method of forming colored images is disclosed. A silver halide color photographic photosensitive material which contains at least one yellow coupler represented by formula (I) in a yellow color forming silver halide emulsion layer which is established on a support is exposed using a scanning exposure system in which the exposure time per picture element is shorter than 10.sup.-4 seconds and then subjected to color development processing: ##STR1## wherein A represents ##STR2## X represents an organic group which is required to form, along with the nitrogen atom, a nitrogen containing heterocyclic ring, Y represents an aromatic group or a heterocyclic group, and Z represents a group which is eliminated on reaction of the coupler represented by formula (I) with an oxidation product of a developing agent. The method provides high quality hard copy both cheaply and quickly by means of a scanning exposure using high density light such as lasers for example. It also provides a method of forming colored images which is improved in respect of the variation in photographic performance with respect to fluctuations in development processing.
    • 公开了形成彩色图像的方法。 在建立在支持体上的黄色形成卤化银乳剂层中含有至少一个由式(I)表示的黄色成色剂的卤化银彩色照相感光材料使用扫描曝光系统曝光,其中每个图像元素的曝光时间 短于10-4秒,然后进行显色处理:其中A表示X表示与氮原子一起形成含氮杂环所需的有机基团,其中A表示< Y表示芳基或杂环基,Z表示通过式(I)表示的成色剂与显影剂的氧化物反应而除去的基团。 该方法通过使用诸如激光器的高密度光的扫描曝光来廉价且快速地提供高质量的硬拷贝。 它还提供了一种形成彩色图像的方法,该方法相对于显影处理的波动而改进了照相性能的变化。
    • 6. 发明授权
    • Silver halide photographic material and color image forming process
    • 卤化银照相材料和彩色成像工艺
    • US5580708A
    • 1996-12-03
    • US303734
    • 1994-09-09
    • Kiyoshi KawaiMario Aoki
    • Kiyoshi KawaiMario Aoki
    • G03C1/79G03C1/83G03C1/46
    • G03C1/832G03C1/79
    • A silver halide photographic material is disclosed, which has on a reflective support at least one light-sensitive layer containing silver halide emulsion grains, wherein said reflective support has at least one waterproof resin coated layer which contains at least 2 g/m.sup.2 of a white pigment in waterproof resin coated layer at the light-sensitive layer coated side and further at least one light-sensitive layer contains at least one compound represented by following general formula (I) in a molecular dispersion state of a monomolecule or a dimer; ##STR1## wherein R.sub.1 to R.sub.4 each represent a hydrogen atom or a substituent, the sum total of the atomic weights of at least one of (R.sub.1 +R.sub.3) and (R.sub.2 +R.sub.4) being not more than 160; n represents 0, 1, or 2; and M represents a hydrogen atom or an alkali metal.
    • 公开了一种卤化银照相材料,其在反射支撑件上具有至少一个含有卤化银乳剂颗粒的感光层,其中所述反射性支撑体具有至少一层防水树脂涂层,其含有至少2g / m 2的白色 在感光层包覆侧的防水树脂涂层中的颜料以及至少一个感光层在分子分子状态或二聚体中含有至少一种由以下通式(I)表示的化合物;氢 原子或取代基,(R1 + R3)和(R2 + R4)中的至少一个的原子量的总和不大于160; n表示0,1或2; M表示氢原子或碱金属。
    • 8. 发明授权
    • Process for producing olefin polymers
    • 生产烯烃聚合物的方法
    • US5463000A
    • 1995-10-31
    • US966850
    • 1992-10-27
    • Yoshihiro MiyoshiHiroyuki ShiraishiKiyoshi KawaiHideaki Koga
    • Yoshihiro MiyoshiHiroyuki ShiraishiKiyoshi KawaiHideaki Koga
    • C08F2/34C08F4/60C08F4/658C08F10/00C08F4/656
    • C08F10/00Y10S526/901Y10S526/904
    • A process for producing an olefin polymer which Comprises homopolymerizing or copolymerizing an olefin in a gas phase by using a catalyst system comprising the following components A) and B):A) a solid catalyst component prepared by immobilizing a catalyst component comprising at least titanium, magnesium, halogen and an electron-donative compound on a porous polymer material, said solid catalyst component satisfying the following conditions (a), (b) and (c):(a) ethylene is preliminarily polymerized thereon in an amount of 50-200 g per gram of the component previously immobilized on the porous polymer material.(b) the preliminary polymerization of ethylene is carried out at most at a rate of 3 g/g-immobilized component-hour at least until the quantity of the preliminarily polymerized ethylene reaches 5 g/g-immobilized component, and(c) the preliminarily polymerized ethylene polymer has an intrinsic viscosity [.eta.] of 0.5 dl/g to 2.5 dl/g as measured in tetralin at 135.degree. C.; andB) an organoaluminum compound.Thus, an olefin polymer small in the extent of adhesion to polymerization reactor, small in the number of fish-eyes, low in the content of fine powder, high in bulk density and good in fluidity could be produced.
    • 一种制备烯烃聚合物的方法,其包括通过使用包含以下组分的催化剂体系在气相中均聚或共聚烯烃A)和B):A)固体催化剂组分,其通过将至少包含钛, 镁,卤素和电子给体化合物,所述固体催化剂组分满足以下条件(a),(b)和(c):(a)乙烯在其上预先聚合的量为50-200 g每克以前固定在多孔聚合物材料上的组分。 (b)乙烯的预聚合至多以3g / g固定成分时间的速度进行至少直到预先聚合的乙烯的量达到5g / g固定成分为止,(c) 预聚合的乙烯聚合物在135℃下在四氢化萘中测得的特性粘度η为0.5dl / g至2.5dl / g。 和B)有机铝化合物。 因此,可以产生在聚合反应器附着程度小,鱼眼数量小,细粉含量低,体积密度高,流动性好的烯烃聚合物。
    • 10. 发明授权
    • Full color recording materials and a method of forming colored images
    • 全色记录材料和形成彩色图像的方法
    • US5185236A
    • 1993-02-09
    • US729910
    • 1991-07-15
    • Keisuke ShibaKiyoshi KawaiMasaki OkazakiYoshiharu Okino
    • Keisuke ShibaKiyoshi KawaiMasaki OkazakiYoshiharu Okino
    • G03C1/035G03C1/12G03C1/28G03C5/16G03C7/30
    • G03C1/035G03C1/28G03C5/164G03C7/30G03C1/12G03C2001/03511G03C2001/03517G03C2001/03535Y10S430/145
    • A full color recording material which has, on a support, at least three silver halide photosensitive emulsion layers which have different color sensitivities and which contain a yellow coupler, magenta coupler and cyan coupler, respectively, and in which at least two of these layers are selectively spectrally sensitized to match semiconductor laser light beams of wavelengths greater than 670 nm, wherein said at least three silver halide photosensitive layers which have different color sensitivities each contains silver chlorobromide grains with a layer average silver chloride content of at least 96 mol %, and said silver chlorobromide grains have a silver bromide local phase of which the silver bromide content is higher than that of the surroundings and a method for forming color images wherein the recording material is imagewise exposed while being transported at a feed rate which matches the scanning rate with semiconductor light beams, and substantially continuously to the exposing, the material is subjected to a color development process wherein the time for color development using a color development solution is not more than 60 seconds, and the time for whole color development process including color development, breach-fixing, washing and/or stabilizing is not more than 180 seconds.
    • 一种全色记录材料,其在载体上具有分别具有不同颜色敏感度并且分别含有黄色成色剂,品红色成色剂和青色成色剂的至少三种卤化银感光乳剂层,并且其中至少两层是 选择性地光谱增感以匹配波长大于670nm的半导体激光束,其中具有不同颜色敏感度的所述至少三个卤化银感光层各自含有平均氯化银含量为至少96mol%的氯化银银粒子,以及 所述氯化溴银颗粒具有溴化银局部相,其溴化银含量高于周围环境的溴化银含量,以及形成彩色图像的方法,其中记录材料在以与扫描速率匹配的进料速率传输时成像曝光, 半导体光束,并且基本上连续地暴露于曝光, 对该材料进行显色处理,其中使用显色溶液的显色时间不超过60秒,并且包括显色,破裂固定,洗涤和/或稳定的整个显色过程的时间不是 超过180秒。