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    • 1. 发明授权
    • Acetolactate synthase gene promoter
    • 乙酰乳酸合酶基因启动子
    • US07238864B2
    • 2007-07-03
    • US11075808
    • 2005-03-10
    • Seiichi TokiHiroaki IchikawaHidemitsu NakamuraKiyoshi KawaiKoichiro KakuTsutomu Shimizu
    • Seiichi TokiHiroaki IchikawaHidemitsu NakamuraKiyoshi KawaiKoichiro KakuTsutomu Shimizu
    • C07H21/04C12N5/05C12N15/09A01H1/00A01H5/00
    • C12N15/8274C12N15/8222
    • The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.
    • 本发明提供诱导组织特异性表达的方法,其包括制备具有启动子的表达载体。 启动子包括以下DNA(a)至(c)中的任一种:(a)包含SEQ ID NO:1所示核苷酸序列的DNA; (b)DNA,其包含从SEQ ID NO:1的核苷酸1344至2843的核苷酸序列; 和(c)DNA,其是在严格条件下与包含与SEQ ID NO:1所示的核苷酸序列完全互补的核苷酸序列的DNA片段杂交的DNA片段,其中,(a),(b)或( c)可以控制位于其下游的编码序列的转录; 并且其中严格条件在42℃为5×SSC; 表达载体也包括位于启动子下游的编码序列。 此外,该方法包括将表达载体导入植物细胞以获得转基因植物; 其中成熟种子或根系中的启动子活性低于在成熟种子或根基以外的植物组织中的活性。 本发明还提供含有启动子的转化体; 启动子包括上述DNA(a)至(c)中的任一种。