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1. 发明申请

WO2023044100A1 MULTITRAN SCENIC PIGS COMPRISING TEN GENETIC MODIFICATIONS FOR XENOTRANSPLANTATION 审中-公开
公开(公告)号：WO2023044100A1
公开(公告)日：2023-03-23
申请号：PCT/US2022/043967
申请日：2022-09-19
申请人： REVIVICOR, INC.
发明人： KOKKINAKI, Maria , KURAVI, Kasinath V.
IPC分类号： A01K67/027 , C12N15/877 , C07K14/705 , C07K14/745 , C12N15/85 , C12N15/90
摘要： The present invention provides transgenic animals (e.g., transgenic porcine animals), organs, tissues, and cells derived from the transgenic animals that are particularly useful for xenotransplantation therapies. In particular, the present invention provides transgenic porcine animals, as well as organs, tissues and cells derived from the transgenic porcine animals, which lack any expression of a functional alpha 1,3 galactosyltransferase (GTKO) gene and comprise at least six transgenes under the control of at least three promoters within a single multi-gene expression vector, and further comprise at least four additional genetic modifications. Also provided are methods of making the transgenic animals (e.g., transgenic porcine animals), and methods of using the transgenic animals, organs, tissues, and cells derived from the transgenic animals for xenotransplantation therapies and treating a disease or condition.

2. 发明申请

WO2019195738A1 COMPOSITIONS AND METHODS FOR SOMATIC CELL REPROGRAMMING AND MODULATING IMPRINTING 审中-公开
公开(公告)号：WO2019195738A1
公开(公告)日：2019-10-10
申请号：PCT/US2019/026074
申请日：2019-04-05
申请人： CHILDREN'S MEDICAL CENTER CORPORATION
发明人： ZHANG, Yi
IPC分类号： A01K67/027 , C12N9/02 , C12N15/113 , C12N15/85 , C12N15/873 , C12N15/877
摘要： The invention provides methods for improving cloning efficiency and modulating an imprinting control region. In particular embodiments, the invention provides methods for activating a repressed allele within an imprinting control region, thereby treating an imprinting associated disorder. In other embodiments, the invention provides methods for improving somatic cell nuclear transfer efficiency that involve Kdm4d overexpression is a Xist knockout donor cell.

3. 发明申请

WO2019154437A1 CRISPR/Cas9载体组合及其在基因敲除中的应用审中-公开
公开(公告)号：WO2019154437A1
公开(公告)日：2019-08-15
申请号：PCT/CN2019/078060
申请日：2019-03-14
申请人：南京医科大学
发明人：戴一凡 , 杨海元 , 王盈
IPC分类号： C12N15/113 , C12N15/63 , C12N15/90 , C12N15/66 , C12N15/877 , A01K67/027
摘要：提供了SgRNA组合，包括特异性靶向GGTA1基因的SgRNA、特异性靶向CMAH基因的SgRNA和特异性靶向β4GalNT2基因的SgRNA；还提供了CRISPR/Cas9载体组合，包括GGTA1-CRISPR/Cas9载体、CMAH-CRISPR/Cas9载体和β4GalNT2-CRISPR/Cas9载体；还提供了CRISPR/Cas9载体组合在敲除GGTA1基因、CMAH基因和β4GalNT2基因中的应用。通过设计特异性靶向的SgRNA序列，三个基因敲除效率分别为56％、63％和41％，敲除与免疫排斥反应有关的三个基因，得到三基因敲除猪，并取得其心脏瓣膜。

4. 发明申请

WO2018086583A1 GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERIC LAG-3 审中-公开
标题翻译：人类或嵌合LAG-3基因修饰的非人类动物
公开(公告)号：WO2018086583A1
公开(公告)日：2018-05-17
申请号：PCT/CN2017/110435
申请日：2017-11-10
申请人： BEIJING BIOCYTOGEN CO., LTD
发明人： SHEN, Yuelei , GUO, Yanan , GUO, Chaoshe , BAI, Yang , YAO, Jiawei , ZHAO, Lei , HUANG, Rui
IPC分类号： A01K67/027 , C12N15/877 , C12N15/90 , C07K14/47 , C07K14/705 , A61K49/16
CPC分类号： A61K49/0008 , A01K67/0278 , A01K2207/15 , A01K2217/072 , A01K2217/15 , A01K2227/105 , A01K2267/03 , A61K2039/505 , A61K2039/507 , C07K14/70503 , C07K16/2803 , C07K16/2818 , C07K2317/24 , C07K2317/76 , C12N15/1138 , C12N15/8509 , C12N15/90 , C12N2310/20
摘要： Provided is a genetically modified non-human animal that expresses a human or chimeric (e.g., humanized) Lymphocyte Activation Gene 3 (LAG-3), and a method of determining effectiveness of an anti-LAG-3 antibody for the treatment of cancer or a tumor by administering the anti-LAG-3 antibody to the animal.
摘要翻译：提供了表达人或嵌合（例如，人源化）淋巴细胞活化基因3（LAG-3）的经遗传修饰的非人动物，以及测定抗LAG- 3抗体用于通过向动物施用抗LAG-3抗体来治疗癌症或肿瘤。

5. 发明申请

WO2016033002A1 SYSTEM AND METHOD OF ENHANCING REPRODUCTION IN AVIAN 审中-公开
标题翻译：增强AVIAN生殖系统与方法
公开(公告)号：WO2016033002A1
公开(公告)日：2016-03-03
申请号：PCT/US2015/046641
申请日：2015-08-25
申请人： GRAJCAR, Zdenko , LILLY, John , PAYNE, Kevin
发明人： GRAJCAR, Zdenko , LILLY, John , PAYNE, Kevin
IPC分类号： C12N15/877
CPC分类号： A01K45/007 , A01K31/22 , A01K67/02 , A61D19/02 , F21V3/00 , F21V29/77 , F21V29/87 , F21V31/005 , F21Y2115/10 , H05B33/0845 , H05B33/0857 , H05B37/0272 , H05B37/0281
摘要： Methods and systems of promoting fertilization of eggs of an avian. While inseminating a plurality of avian eggs a spectrum substantially concentrated within a narrow range of wavelength irradiates the plurality of inseminated avian eggs to increase the motility of the sperm to increase the probability of conception of avian from the inseminated eggs.
摘要翻译：促进禽类卵子受精的方法和系统。虽然对多个禽蛋进行授精，但基本上集中在窄波长范围内的光谱照射多个受精的禽卵，以增加精子的运动性，从而增加受精卵的鸟类受孕概率。

6. 发明申请

WO2015162170A1 IMPROVED METHOD FOR RECONSTRUCTING A NON-HUMAN ANIMAL EMBRYO 审中-公开
标题翻译：改进非人类动物胚胎的改进方法
公开(公告)号：WO2015162170A1
公开(公告)日：2015-10-29
申请号：PCT/EP2015/058701
申请日：2015-04-22
申请人： LOI, Pasqualino , IUSO, Domenico , CZERNIK, Marta , ZACCHINI, Federica , PTAK, Grazyna , KHOCHBIN, Saadi , FIDANZA, Antonella
发明人： LOI, Pasqualino , IUSO, Domenico , CZERNIK, Marta , ZACCHINI, Federica , PTAK, Grazyna , KHOCHBIN, Saadi , FIDANZA, Antonella
IPC分类号： C12N15/873 , C12N15/877 , A01K67/027
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/00 , A01K2227/103 , C12N15/877 , C12N15/8773
摘要： The present invention refers to a method for reconstructing a non-human animal embryo, to a method for generating a non-human animal and to a non-human animal or non-human animal reconstructed embryo obtainable by the methods according to the invention. The method of somatic cell nuclear transfer (SCNT) comprises a step of transiently expressing or inducing the uptake of a protein involved in male sperm maturation, such as protamine or transition protein, in the somatic donor cell before nuclear transfer.
摘要翻译：本发明涉及一种用于重建非人动物胚胎的方法，用于产生非人动物的方法和通过本发明的方法获得的非人动物或非人动物重建胚胎的方法。体细胞核转移（SCNT）的方法包括在核转移之前在体细胞供体细胞中瞬时表达或诱导涉及雄性精子成熟的蛋白质（例如鱼精蛋白或转换蛋白）的步骤。

7. 发明申请

WO2012071762A1 制备抗蓝耳病转基因猪的方法审中-公开
公开(公告)号：WO2012071762A1
公开(公告)日：2012-06-07
申请号：PCT/CN2011/000274
申请日：2011-02-22
申请人：北京济福霖生物技术有限公司 , 鲍永华 , 郭永臣 , 李秋燕 , 汤波 , 李宁
发明人：鲍永华 , 郭永臣 , 李秋燕 , 汤波 , 李宁
IPC分类号： C12N5/10 , C12N15/877 , C12N15/113 , C12N15/40 , A01K67/027 , C12R1/93
CPC分类号： A01K67/0275 , A01K2217/00 , A01K2227/108 , A01K2267/02 , C12N15/1131 , C12N2310/14

8. 发明申请

WO2005033274A3 A PROCESS FOR PRODUCING EXOGENOUS PROTEIN IN THE MILK OF TRANGENIC MAMMALS AND A PROCESS FOR PURIFYING PROTEINS THEREFROM 审中-公开
标题翻译：一种在变性乳制品中生产异
质蛋白的方法及其净化蛋白质的方法
公开(公告)号：WO2005033274A3
公开(公告)日：2005-07-14
申请号：PCT/US2004031819
申请日：2004-09-29
申请人： STERRENBELD BIOTECHNOLOGIE NORTH AMERICA INC , MELO CARLOS ALBERTO , BARANAO LINO , CARBONNETTO CESAR
发明人： MELO CARLOS ALBERTO , BARANAO LINO , CARBONNETTO CESAR
IPC分类号： A01K67/027 , C07K1/00 , C07K14/00 , C07K14/61 , C07K16/00 , C07K17/00 , C12N20060101 , C12N15/00 , C12N15/85 , C12N15/877 , C12P21/00
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , C07K14/61 , C12N15/8509 , C12N15/8771 , C12N2830/008
摘要： The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.
摘要翻译：本发明涉及产生感兴趣的蛋白质的方法，包括制备在其乳中产生所述蛋白质的非人转基因哺乳动物，从非人转基因哺乳动物获得所述乳，并从所述牛奶中纯化所述目的蛋白质。产生转基因牛动物，其能够在乳腺中产生人生长激素。该方法包括在表达载体中方便地克隆编码hGH基因和β酪蛋白启动子的遗传构建体。它还包括将转染过程转化为胎牛体细胞，通常是成纤维细胞，以及将核转移到去核牛卵母细胞中，从而产生转基因胚胎。该方法还包括产生用于进一步扩增转基因畜群的转基因胚胎的其它方法，例如转基因雌性牛的亚克隆，转基因奶牛的超排卵及其来自非转基因或转基因雄性牛的精液授精，以及非转基因奶牛的超排卵及其来自转基因雄性牛的精液的授精随后，转基因胚胎产生大量在其乳中产生人生长激素的转基因牛，其中激素被完全纯化并分析以完成所有制造纯生物制药产品的要求。

9. 发明申请

WO2004105477A3 METHOD FOR CLONING IN THE RAT BY NUCLEAR TRANSFER 审中-公开
标题翻译：通过核转运克隆大鼠的方法
公开(公告)号：WO2004105477A3
公开(公告)日：2005-02-03
申请号：PCT/FR2004001275
申请日：2004-05-24
申请人： AGRONOMIQUE INST NAT RECH , COZZI JEAN , ZHOU QI , RENARD JEAN-PAUL
发明人： COZZI JEAN , ZHOU QI , RENARD JEAN-PAUL
IPC分类号： C12N5/075 , C12N15/06 , C12N15/877 , A01K67/027 , C12N5/06
CPC分类号： C12N15/8775 , A01K2217/05 , C12N5/0609 , C12N2501/70
摘要： The invention relates to a method for cloning in the rat by nuclear transfer. The invention further relates to the rats obtained thus, in the foetal or adult state, as well as use thereof for the production of molecules of interest or as study models.
摘要翻译：本发明涉及通过核转移在大鼠中克隆的方法。本发明还涉及以胎儿或成年状态获得的大鼠以及其用于生产感兴趣分子或作为研究模型的用途。

10. 发明申请

WO03005810A9 CLONED NON-HUMAN MAMMALS FROM CONTACT INHIBITED DONOR CELLS 审中-公开
标题翻译：从接触抑制的细胞中克隆非人类哺乳动物
公开(公告)号：WO03005810A9
公开(公告)日：2004-02-26
申请号：PCT/US0221680
申请日：2002-07-09
申请人： ALEXION PHARMA INC
发明人： FODOR WILLIAM , RAMPSOONDAR JAGDEECE , BONDIOLI KENNETH R
IPC分类号： C12N15/873 , C12N15/877 , G01N33/00 , A01K67/00 , C12N15/00
CPC分类号： C12N15/873 , C12N15/877
摘要： Cloned non-human mammalian nuclear transfer units, embryos and animals are provided by introducing a contact-inhibited non-cycling mammalian donor cell in the G1 state or a nucleus thereof into an enucleated oocyte from a non-human mammal of the same species as the donor to form a nuclear transfer unit, implanting the nuclear transfer unit into the uterus of a surrogate mother, permitting the nuclear transfer unit to develop into the cloned non-human mammalian embryo and allowing the embryo to come to term.
摘要翻译：克隆的非人哺乳动物核转移单位，胚胎和动物通过将G1状态或其核中的接触抑制非循环哺乳动物供体细胞引入与来自相同种类的非人哺乳动物的去核卵母细胞供体形成核转移单元，将核转移单元植入代孕母亲的子宫，允许核转移单位发育成克隆的非人哺乳动物胚胎并允许胚胎进入期限。

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