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1. 发明申请

WO2005038001A3 IMPROVED TRANSGENESIS BY SPERM-MEDIATED GENE TRANSFER 审中-公开
标题翻译：通过精准介导的基因转移改善转基因
公开(公告)号：WO2005038001A3
公开(公告)日：2005-09-15
申请号：PCT/US2004033954
申请日：2004-10-13
申请人： THERAPEUTIC HUMAN POLYCLONALS , BUELOW ROLAND
发明人： BUELOW ROLAND
IPC分类号： A01K67/00 , A01K67/027 , C07H21/04 , C07K14/705 , C12N20060101 , C12N15/00 , C12N15/85 , C12N15/86 , C12N15/877 , C12N15/90 , C12N15/87
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C07K14/70503 , C12N15/8773 , C12N15/907 , C12N2800/204
摘要： The invention relates to improved methods to integrate heterologous DNA into the genome of non-human animals. The transgenic constructs contain a site recognized by a site specific recombinase which can be used to catalyze the insertion of the transgene into the animal's genome. The transgenic constructs and site specific recombinase are introduced into oocytes using sperm. Fertilized oocytes are maintained under conditions that allow recombinase-mediated integration of the transgene into the genome of the non-human animal and development to term. Transgenic animals with transgenes integrated by a recombinase express transgenes at higher levels compared to transgenic animals with randomly integrated transgenes.
摘要翻译：本发明涉及将异源DNA整合到非人动物基因组中的改进方法。转基因构建体含有被位点特异性重组酶识别的位点，该位点可用于催化转基因插入动物基因组。使用精子将转基因构建体和位点特异性重组酶导入卵母细胞。将受精的卵母细胞维持在允许重组酶介导的转基因整合入非人动物基因组并且发育至足月的条件下。与具有随机整合的转基因的转基因动物相比，具有由重组酶整合的转基因的转基因动物以更高的水平表达转基因。

2. 发明申请

WO01083731A1 METHODS AND COMPOSITIONS FOR PRODUCING DONOR CELLS 审中-公开
标题翻译：用于生产细胞的方法和组合物
公开(公告)号：WO01083731A1
公开(公告)日：2001-11-08
申请号：PCT/US2001/013669
申请日：2001-04-27
IPC分类号： A61K35/12 , C12N15/873 , C12N15/877 , C12N15/00 , C12N15/63 , C12N15/85 , C12N15/87
CPC分类号： C12N15/873 , A61K35/12 , C12N15/877 , C12N15/8773 , C12N2517/04
摘要： Methods and compositions are provided for producing at least one donor cell that has a genome identical to that of a host. In the subject methods, an embryo is first produced by combining a nucleus obtained from a cell of said host with a recipient cell, e.g., an enucleated oocyte. The resultant embryo is then matured under conditions sufficient to produce a non-sentient organism that includes the donor cell. Finally, the donor cell is harvested from the non-sentient organism for subsequent use. Also provided are kits for use in practicing the subject invention. The subject methods find use in a variety of different applications, including tissue and organ transplant applications.
摘要翻译：提供了用于产生至少一个具有与宿主基因组相同的基因组的供体细胞的方法和组合物。在本发明方法中，首先通过将从所述宿主的细胞获得的细胞核与受体细胞（例如去核卵母细胞）组合来产生胚胎。然后在足以产生包括供体细胞的非感兴趣生物体的条件下，使得到的胚胎成熟。最后，从非有意识的生物体收获供体细胞以供随后使用。还提供了用于实践本发明的试剂盒。主题方法可用于各种不同的应用，包括组织和器官移植应用。

3. 发明申请

WO2015162170A1 IMPROVED METHOD FOR RECONSTRUCTING A NON-HUMAN ANIMAL EMBRYO 审中-公开
标题翻译：改进非人类动物胚胎的改进方法
公开(公告)号：WO2015162170A1
公开(公告)日：2015-10-29
申请号：PCT/EP2015/058701
申请日：2015-04-22
申请人： LOI, Pasqualino , IUSO, Domenico , CZERNIK, Marta , ZACCHINI, Federica , PTAK, Grazyna , KHOCHBIN, Saadi , FIDANZA, Antonella
发明人： LOI, Pasqualino , IUSO, Domenico , CZERNIK, Marta , ZACCHINI, Federica , PTAK, Grazyna , KHOCHBIN, Saadi , FIDANZA, Antonella
IPC分类号： C12N15/873 , C12N15/877 , A01K67/027
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/00 , A01K2227/103 , C12N15/877 , C12N15/8773
摘要： The present invention refers to a method for reconstructing a non-human animal embryo, to a method for generating a non-human animal and to a non-human animal or non-human animal reconstructed embryo obtainable by the methods according to the invention. The method of somatic cell nuclear transfer (SCNT) comprises a step of transiently expressing or inducing the uptake of a protein involved in male sperm maturation, such as protamine or transition protein, in the somatic donor cell before nuclear transfer.
摘要翻译：本发明涉及一种用于重建非人动物胚胎的方法，用于产生非人动物的方法和通过本发明的方法获得的非人动物或非人动物重建胚胎的方法。体细胞核转移（SCNT）的方法包括在核转移之前在体细胞供体细胞中瞬时表达或诱导涉及雄性精子成熟的蛋白质（例如鱼精蛋白或转换蛋白）的步骤。

4. 发明申请

WO2003097812A2 TRANSGENIC UNGULATES CAPABLE OF HUMAN ANTIBODY PRODUCTION 审中-公开
标题翻译：人类抗体生产的转基因生物
公开(公告)号：WO2003097812A2
公开(公告)日：2003-11-27
申请号：PCT/US2003/015937
申请日：2003-05-19
申请人： HEMATECH, LLC , KIRIN BEER KABUSHIKI KAISHA , ROBL, James, M. , COLLAS, Philippe , SULLIVAN, Eddie , KASINATHAN, P. , GOLDSBY, Richard, A. , KUROIWA, Yoshimi , TOMIZUKA, Kazuma , ISHIDA, Isao
发明人： ROBL, James, M. , COLLAS, Philippe , SULLIVAN, Eddie , KASINATHAN, P. , GOLDSBY, Richard, A. , KUROIWA, Yoshimi , TOMIZUKA, Kazuma , ISHIDA, Isao
IPC分类号： C12N
CPC分类号： A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2227/101 , A01K2267/01 , C12N15/8509 , C12N15/8773
摘要： The invention features novel methods for the production of large quantities of xenogenous antibodies, such as human antibodies. Preferably, this result is effected by inactivation of IgM heavy chain expression and, optionally, by inactivation of Ig light chain expression, and by the further introduction of an artificial chromosome which results in the expression of xenogenous antibodies (e.g., non-bovine antibodies), preferably human antibodies.
摘要翻译：本发明的特征在于产生大量异种抗体（例如人抗体）的新方法。优选地，该结果通过IgM重链表达的失活和任选地通过Ig轻链表达的失活以及进一步引入导致异源抗体（例如非牛抗体）的表达的人造染色体来实现，，优选人抗体。

5. 发明申请

WO2005038001A2 IMPROVED TRANSGENESIS BY SPERM-MEDIATED GENE TRANSFER 审中-公开
标题翻译：通过培养的基因转移改进转化
公开(公告)号：WO2005038001A2
公开(公告)日：2005-04-28
申请号：PCT/US2004/033954
申请日：2004-10-13
申请人： THERAPEUTIC HUMAN POLYCLONALS, INC. , BUELOW, Roland
发明人： BUELOW, Roland
IPC分类号： C12N
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C07K14/70503 , C12N15/8773 , C12N15/907 , C12N2800/204
摘要： The invention relates to improved methods to integrate heterologous DNA into the genome of non-human animals. The transgenic constructs contain a site recognized by a site specific recombinase which can be used to catalyze the insertion of the transgene into the animal's genome. The transgenic constructs and site specific recombinase are introduced into oocytes using sperm. Fertilized oocytes are maintained under conditions that allow recombinase-mediated integration of the transgene into the genome of the non-human animal and development to term. Transgenic animals with transgenes integrated by a recombinase express transgenes at higher levels compared to transgenic animals with randomly integrated transgenes.
摘要翻译：本发明涉及将异源DNA整合到非人动物基因组中的改进方法。转基因构建体含有由位点特异性重组酶识别的位点，其可用于催化将转基因插入到动物基因组中。使用精子将转基因构建体和位点特异性重组酶导入卵母细胞。受精的卵母细胞保持在允许重组酶介导的转基因整合到非人类动物的基因组和发育期的条件下。与具有随机整合的转基因的转基因动物相比，通过重组酶整合的转基因的转基因动物以更高的水平表达转基因。

6. 发明申请

WO2004024227A3 SYSTEMS OF SPERM CELL PRESERVATION AND PROCESSING 审中-公开
标题翻译：电池保存和处理系统
公开(公告)号：WO2004024227A3
公开(公告)日：2004-11-04
申请号：PCT/US0330814
申请日：2003-09-15
申请人： XY INC , MAXWELL WILLIAM MAXWELL CHISHO , HOLLINSHEAD FIONA KATE , O'BRIEN JUSTINE KELLIE , EVANS GARETH
发明人： MAXWELL WILLIAM MAXWELL CHISHO , HOLLINSHEAD FIONA KATE , O'BRIEN JUSTINE KELLIE , EVANS GARETH
IPC分类号： A01K67/027 , A01N1/00 , A01N1/02 , A61N20060101 , C12N5/00 , C12N5/076 , C12N15/00 , C12N15/873 , C12N15/877
CPC分类号： C12N5/0604 , A01K2227/101 , A01K2227/103 , A61B17/43 , C12N5/061 , C12N15/873 , C12N15/8771 , C12N15/8773 , C12N2517/10
摘要： The preservation of sperm cells, and other processing of sperm cells, are disclosed. The present invention is directed in some embodiments to sperm cell preservation, as sperm cell preservation may further relate to artificial insemination, in vitro fertilization, super-ovulation, mammal production, sperm samples, and sperm cell processing, generally. The present invention is also directed in some embodiments to sperm cell processing features, such as collecting, handling, sorting, storage, transportation, use, fertilization, or insemination techniques. Sperm cell preservation in accordance with the present invention may address maintenance or enhancement of sperm quality, and in some embodiments one or more sperm cell characteristics, such as viability, motility, and functionality. Fertilization rates may also be maintained or enhanced. An embodiment may comprise obtaining sperm cells (10). The sperm cells may be cryoperserved (20) and thawed (30). Processing (40) may be performed on the sperm cells and cryopreservation of the sperm cells (50).

7. 发明申请

WO2007010287A1 BIOLOGICAL MATERIALS AND USES THEREOF 审中-公开
标题翻译：生物材料及其用途
公开(公告)号：WO2007010287A1
公开(公告)日：2007-01-25
申请号：PCT/GB2006/002754
申请日：2006-07-21
申请人： THE UNIVERSITY OF NOTTINGHAM , JOHNSON, Andrew , CAMPBELL, Keith , ALBERIO, Ramiro
发明人： JOHNSON, Andrew , CAMPBELL, Keith , ALBERIO, Ramiro
IPC分类号： C12N5/06 , A61K35/56 , C12N5/08
CPC分类号： C12N5/16 , A61K35/60 , A61K35/65 , C07K14/4702 , C12N5/0696 , C12N15/8773 , C12N2501/605 , C12N2502/04 , C12N2506/00 , C12N2517/10
摘要： A method of producing a reprogrammed cell or reprogrammed cell nucleus, comprising exposing a differentiated cell, or the nucleus of a differentiated cell to a cell or cell extract thereof derived from an oocyte, egg, ovary or early embryo of a cold blooded vertebrate, wherein the cold blooded vertebrate has one or more of the following properties: (i) a primitive vertebrate body plan including laterally projecting ribs and/or spinal projections; (ii) germ cells which do not contain germ plasm; and/or (iii) the oocyte, egg, ovary or early embryo cell or cell from which the cell extract is derived, expresses a highly conserved form of Oct-4 and/or nanog. There is also provided uses of the reprogrammed cells.
摘要翻译：一种生产重编程细胞或重编程细胞核的方法，包括将分化细胞或分化细胞的核暴露于衍生自冷血脊椎动物的卵母细胞，卵，卵巢或早期胚胎的细胞或细胞提取物中，其中冷血脊椎动物具有以下一个或多个性质：（i）包括横向突出的肋和/或脊柱突起的原始脊椎动物体平面图; （ii）不含生殖细胞的生殖细胞; 和/或（iii）来自细胞提取物的卵母细胞，卵，卵巢或早期胚胎细胞或细胞，表达高度保守形式的Oct-4和/或纳米。还提供了重编程细胞的用途。

8. 发明申请

WO2004024227A2 SYSTEMS OF SPERM CELL PRESERVATION AND PROCESSING 审中-公开
标题翻译：电池保存和处理系统
公开(公告)号：WO2004024227A2
公开(公告)日：2004-03-25
申请号：PCT/US2003/030814
申请日：2003-09-15
申请人： XY, INC. , MAXWELL, William, Maxwell, Chisholm , HOLLINSHEAD, Fiona, Kate , O'BRIEN, Justine, Kellie , EVANS, Gareth
发明人： MAXWELL, William, Maxwell, Chisholm , HOLLINSHEAD, Fiona, Kate , O'BRIEN, Justine, Kellie , EVANS, Gareth
IPC分类号： A61N
CPC分类号： C12N5/0604 , A01K2227/101 , A01K2227/103 , A61B17/43 , C12N5/061 , C12N15/873 , C12N15/8771 , C12N15/8773 , C12N2517/10
摘要： The preservation of sperm cells, and other processing of sperm cells, are disclosed. The present invention is directed in some embodiments to sperm cell preservation, as sperm cell preservation may further relate to artificial insemination, in vitro fertilization, super-ovulation, mammal production, sperm samples, and sperm cell processing, generally. The present invention is also directed in some embodiments to sperm cell processing features, such as collecting, handling, sorting, storage, transportation, use, fertilization, or insemination techniques. Sperm cell preservation in accordance with the present invention may address maintenance or enhancement of sperm quality, and in some embodiments one or more sperm cell characteristics, such as viability, motility, and functionality. Fertilization rates may also be maintained or enhanced. An embodiment may comprise obtaining sperm cells (10). The sperm cells may be cryoperserved (20) and thawed (30). Processing (40) may be performed on the sperm cells and cryopreservation of the sperm cells (50).
摘要翻译：公开了精子细胞的保存和精子细胞的其他处理。本发明在一些实施方案中针对精子细胞保存，因为精子细胞保存可进一步涉及人工授精，体外受精，超排卵，哺乳动物生产，精子样品和精子细胞处理。本发明还在一些实施方案中涉及精子细胞处理特征，例如收集，处理，分选，储存，运输，使用，受精或授精技术。根据本发明的精子细胞保存可以解决精子质量的维持或增强，并且在一些实施方案中可以涉及一种或多种精子细胞特征，例如活力，运动性和功能性。受精率也可以维持或提高。一个实施方案可以包括获得精子细胞（10）。精液细胞可能被冷冻（20）并解冻（30）。可以对精子细胞进行处理（40），并对精子细胞进行冷冻保存（50）。

9. 发明申请

WO1997007669A1 QUIESCENT CELL POPULATIONS FOR NUCLEAR TRANSFER 审中-公开
标题翻译：用于核转运的细胞群体
公开(公告)号：WO1997007669A1
公开(公告)日：1997-03-06
申请号：PCT/GB1996002099
申请日：1996-08-30
申请人： ROSLIN INSTITUTE (EDINBURGH) , CAMPBELL, Keith, Henry, Stockman , WILMUT, Ian
发明人： ROSLIN INSTITUTE (EDINBURGH)
IPC分类号： A01K67/027
CPC分类号： C12N15/8771 , A61K35/54 , C12N15/8773
摘要： A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
摘要翻译：重建动物胚胎的方法涉及将细胞核从静止供体细胞转移到合适的受体细胞中。供体细胞是静止的，因为它被导致在G1处的生长和分裂周期退出并且在G0状态下停止。核转移可能通过细胞融合发生。然后，重建的胚胎可以产生一种或多种动物。本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

10. 发明申请

WO1997007668A1 UNACTIVATED OOCYTES AS CYTOPLAST RECIPIENTS FOR NUCLEAR TRANSFER 审中-公开
标题翻译：作为用于核转移的CYPOPLAST接收者的活化的OOCYTES
公开(公告)号：WO1997007668A1
公开(公告)日：1997-03-06
申请号：PCT/GB1996002098
申请日：1996-08-30
申请人： ROSLIN INSTITUTE (EDINBURGH) , CAMPBELL, Keith, Henry, Stockman , WILMUT, Ian
发明人： ROSLIN INSTITUTE (EDINBURGH)
IPC分类号： A01K67/027
CPC分类号： C12N15/8773 , A61K35/54 , C12N15/873 , C12N15/8771
摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
摘要翻译：重建动物胚胎的方法涉及将二倍体核转移到在第二次减数分裂期的中期被逮捕的卵母细胞。卵母细胞在转移时不被激活，使得供者核细胞保持暴露于受体细胞质一段时间。二倍体核可以在转移时由细胞G0或G1期的细胞捐献。随后，重建的胚胎被激活。在活化期间维持正确的倍性，例如通过在微管抑制剂例如诺考达唑的存在下孵育重构的胚胎。然后，重建的胚胎可能会产生一种或多种活的动物分娩。本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

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