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1. 发明申请

WO2016044271A2 METHODS AND COMPOSITIONS TO INCREASE SOMATIC CELL NUCLEAR TRANSFER (SCNT) EFFICIENCY BY REMOVING HISTONE H3-LYSINE TRIMETHYLATION 审中-公开
标题翻译：通过去除HISTONE H3-LYSINE TRIMETHYLATION的方法和组合物来提高自身细胞核转运（SCNT）的效率
公开(公告)号：WO2016044271A2
公开(公告)日：2016-03-24
申请号：PCT/US2015050178
申请日：2015-09-15
申请人： CHILDRENS MEDICAL CENTER
发明人： ZHANG YI , MATOBA SHOGO
IPC分类号： A01K67/027 , C12N15/85
CPC分类号： C12N15/877 , A01K67/0273 , A01K2227/105 , C12N5/0604 , C12N5/0606 , C12N15/1137 , C12N2310/14 , C12N2517/04 , C12Y201/01043
摘要： The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.
摘要翻译：本发明提供了提高体细胞核转移（SCNT）的效率以及由此产生核转移ESC（ntESC）和转基因细胞和/或非人动物的方法和组合物。更具体地说，本发明涉及组合物H3-赖氨酸9（H3K9me3）在供体体细胞核遗传物质中的重编程抗性区（RRR）中三甲基化的发现，从而阻止有效的体细胞核重编程或SCNT。本发明提供了通过抑制组蛋白甲基转移酶Suv39h1和/或Suv39h2，通过脱甲基酶Kdm4家族的外源或过表达和/或抑制H3K9me3的甲基化来提高SCNT功效的方法中减少H3K9me3的方法和组合物。

2. 发明申请

WO2011132990A2 TRANSGENIC CLONED CANINE OVEREXPRESSING PEPCK, AND PRODUCTION METHOD THEREOF 审中-公开
标题翻译：转基因克隆重组棉花生产方法及其生产方法
公开(公告)号：WO2011132990A2
公开(公告)日：2011-10-27
申请号：PCT/KR2011002944
申请日：2011-04-22
申请人： HWANG WOO-SUK
发明人： HWANG WOO-SUK
IPC分类号： C12N5/075 , A01K67/027 , C12N5/16 , C12N15/873
CPC分类号： C12N15/8509 , A01K67/0273 , A01K67/0275 , A01K2217/052 , A01K2227/10 , A01K2267/035 , A01K2267/0362
摘要： The present invention relates to a transgenic cloned canine overexpressing phosphoenol pyruvate carboxykinase (PEPCK), and a production method thereof. More specifically, the present invention relates to a nuclear transferred egg of a canine formed by transferring the nucleus of a somatic cell derived from a canine transformed with a PEPCK gene to an enucleated egg, a production method thereof, a transgenic cloned canine overexpressing PEPCK produced by transferring the nuclear transferred egg into the oviduct of a surrogate mother, and a production method thereof. The transgenic cloned canine overexpressing PEPCK gene according to the present invention, as an animal model of diabetes mellitus, can be used for investigating the genetic cause of type 2 diabetes mellitus and developing a diagnostic reagent and a therapeutic agent.
摘要翻译：本发明涉及转基因克隆犬过表达磷酸烯醇丙酮酸羧激酶（PEPCK）及其制备方法。更具体地，本发明涉及通过将衍生自用PEPCK基因转化的犬的体细胞的细胞核转移到去核卵而形成的犬的核转移卵，其生产方法，产生过表达PEPCK的转基因克隆犬通过将核转移的卵转移到替代母亲的输卵管中，及其制造方法。作为糖尿病的动物模型的本发明的过表达PEPCK基因的转基因克隆犬可用于研究2型糖尿病的遗传原因并开发诊断试剂和治疗剂。

3. 发明申请

WO2009111086A1 TRANSGENIC NON-HUMAN MAMMALS WITH KAPPA LIGHT CHAIN OF XENOGENOUS IMMUNOGLOBULIN 审中-公开
标题翻译：具有KAPPA轻链免疫球蛋白轻链的转基因非人类哺乳动物
公开(公告)号：WO2009111086A1
公开(公告)日：2009-09-11
申请号：PCT/US2009/001501
申请日：2009-03-09
申请人： KIRIN PHARMA KABUSHIKI KAISHA , KUROIWA, Yoshimi , TOMIZUKA, Kazuma , ROBL, James, M.
发明人： KUROIWA, Yoshimi , TOMIZUKA, Kazuma , ROBL, James, M.
IPC分类号： C12P21/00
CPC分类号： C07K16/00 , A01K67/0273 , A01K67/0278 , A01K2217/052 , A01K2217/15 , A01K2227/101 , A01K2267/01 , C07K16/1278 , C07K16/18 , C07K2317/21 , C07K2317/41 , C07K2317/50 , C12N15/8509 , C12N2800/30
摘要： Genetically modified non-human mammals (e.g., bovines, porcines, and other ungulates) and cells containing a nucleic acid encoding all or part of a xenogenous kappa immunoglobulin gene capable of undergoing rearrangement and expressing a xenogenous kappa immunoglobulin are described. Antibodies produced from these non-human mammals and cells are described. Methods of making these mammals and cells are presented. In addition, methods of producing xenogenous (e.g., human) antibodies from the genetically modified non-human mammals and cells are described.
摘要翻译：描述了遗传修饰的非人哺乳动物（例如牛，猪和其他有蹄类动物）和含有编码全部或部分能够进行重排和表达异种κ免疫球蛋白的异源κ免疫球蛋白基因的核酸的细胞。描述了从这些非人哺乳动物和细胞产生的抗体。提出制作这些哺乳动物和细胞的方法。此外，描述了从遗传修饰的非人哺乳动物和细胞产生异种（例如，人）抗体的方法。

4. 发明申请

WO2008106985A1 PIG MODEL 审中-公开
标题翻译： PIG型号
公开(公告)号：WO2008106985A1
公开(公告)日：2008-09-12
申请号：PCT/DK2008/050058
申请日：2008-03-07
申请人： AARHUS UNIVERSITET , MIKKELSEN, Jacob Giehm , MOLDT, Brian , NIELSEN, Anders Lade , BOLUND, Lars Axel , KRAGH, Peter, Michael , JAKOBSEN, Jannik Ejnar , JØRGENSEN, Arne, Lund
发明人： MIKKELSEN, Jacob Giehm , MOLDT, Brian , NIELSEN, Anders Lade , BOLUND, Lars Axel , KRAGH, Peter, Michael , JAKOBSEN, Jannik Ejnar , JØRGENSEN, Arne, Lund
IPC分类号： A01K67/027 , C12N5/10 , C12N15/85
CPC分类号： A01K67/0275 , A01K67/0273 , A01K2217/05 , A01K2227/108 , A01K2267/035 , C12N15/8509 , C12N15/8778 , C12N2800/30 , C12N2800/90 , C12N2840/203
摘要： The present invention relates to a genetically modified pig comprising at least one site for integration of at least one transgene. The invention also pertains to a porcine embryo, blastocyst, foetus, donor cell and/or cell nucleus, derived from said genetically modified pig. In another aspect, the invention relates to any genetically modified porcine blastocyst, wherein the genetically modified genome comprises at least one site for integration of at least one transgene.
摘要翻译：本发明涉及包含至少一个用于整合至少一个转基因的位点的转基因猪。本发明还涉及源自所述转基因猪的猪胚，胚泡，胎儿，供体细胞和/或细胞核。另一方面，本发明涉及任何遗传修饰的猪胚泡，其中所述经遗传修饰的基因组包含至少一个用于整合至少一个转基因的位点。

5. 发明申请

WO2006036975A2 METHODS OF EMBRYO TRANSFER 审中-公开
标题翻译：胚胎转移方法
公开(公告)号：WO2006036975A2
公开(公告)日：2006-04-06
申请号：PCT/US2005/034641
申请日：2005-09-28
申请人： VIAGEN, INC. , DAVIS, Scott, K. , WALKER, Shawn , POLEJAEVA, Irina
发明人： DAVIS, Scott, K. , WALKER, Shawn , POLEJAEVA, Irina
IPC分类号： A01K67/027
CPC分类号： A01K67/0273 , A01K2227/108 , A01K2267/02 , A01K2267/025 , C12N15/8778
摘要： Methods for the efficient production of cloned porcine fetuses/piglets following the production of cloned embryos, including culture of the embryos for extended periods prior to transfer of the embryos into the uterus of the recipient. Transfer can be accomplished surgically or through less-invasive laparoscopic or non-surgical transfer.
摘要翻译：在克隆胚胎生产之后有效生产克隆的猪胎儿/仔猪的方法，包括在将胚胎转移到接受者的子宫之前将胚胎长时间培养。转移可以通过手术或通过较少侵入性的腹腔镜或非手术转移来完成。

6. 发明申请

WO0247479A3 CLONING BOVINES BY NUCLEAR TRANSPLANTATION 审中-公开
标题翻译：通过核运输克隆BOVINES
公开(公告)号：WO0247479A3
公开(公告)日：2003-09-04
申请号：PCT/US0148521
申请日：2001-12-14
申请人： TEXAS A & M UNIV SYS , WESTHUSIN MARK E , ADAMS GARRY , HUNTER DORIS , TEMPLETON JOE W , SHIN TAEYOUNG
发明人： WESTHUSIN MARK E , ADAMS GARRY , HUNTER DORIS , TEMPLETON JOE W , SHIN TAEYOUNG
IPC分类号： A01K67/027 , C07K14/47 , C12N15/85 , C12N15/873 , C12N15/877
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/05 , A01K2227/101 , A01K2267/02 , C07K14/47 , C12N15/8509 , C12N15/877
摘要： Nuclear transfer methods and techniques involving cryopreserved bovine somatic cells, nuclei, or nuclear DNA, and the products thereof, are disclosed. These methods employ an enucleated oocyte as a recipient, which is fused with a donor nuclear genome from a cryopreserved bovine somatic cell to form a couplet. In addition, the a cloned bull exhibiting disease resistance is disclosed.
摘要翻译：公开了涉及低温保存的牛体细胞，核或核DNA的核转移方法和技术及其产物。这些方法使用去核卵母细胞作为受体，其与来自冷冻保存的牛体细胞的供体核基因组融合以形成对偶。另外，公开了抗克隆的公牛抗病性。

7. 发明申请

WO02047479A2 CLONING BOVINES BY NUCLEAR TRANSPLANTATION 审中-公开
标题翻译：通过核运输克隆BOVINES
公开(公告)号：WO02047479A2
公开(公告)日：2002-06-20
申请号：PCT/US2001/048521
申请日：2001-12-14
IPC分类号： A01K67/027 , C07K14/47 , C12N15/85 , C12N15/873 , C12N15/877
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/05 , A01K2227/101 , A01K2267/02 , C07K14/47 , C12N15/8509 , C12N15/877
摘要： Nuclear transfer methods and techniques involving cryopreserved bovine somatic cells, nuclei, or nuclear DNA, and the products thereof, are disclosed. These methods employ an enucleated oocyte as a recipient, which is fused with a donor nuclear genome from a cryopreserved bovine somatic cell to form a couplet. In addition, the a cloned bull exhibiting disease resistance is disclosed.
摘要翻译：公开了涉及低温保存的牛体细胞，核或核DNA的核转移方法和技术及其产物。这些方法使用去核卵母细胞作为受体，其与来自冷冻保存的牛体细胞的供体核基因组融合以形成对偶。另外，公开了抗克隆的公牛抗病性。

8. 发明申请

WO02009510A2 SELECTION AND CLONING METHODS 审中-公开
标题翻译：选择和克隆方法
公开(公告)号：WO02009510A2
公开(公告)日：2002-02-07
申请号：PCT/US2001/041561
申请日：2001-08-01
IPC分类号： A01K67/027 , C12N15/85 , C12N15/873
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/05 , A01K2217/20 , A01K2227/101 , A01K2267/02 , C12N15/8509
摘要： Selection and cloning methods are disclosed that are effective for the selective multiplication of desired animals, for instance livestock animals. These methods can be used to expand populations of animals, and are particularly useful for duplicating animals selected based on traits that are measured after the animal is deceased. Certain embodiments include techniques useful for selecting a desirable bovine animal (e.g., desirable steer) based at least in part on a measurable carcass trait. Also described are specific cloning techniques that involve repetitive (for instance, two) cycles of cloning, such as nuclear transfer cloning. In specific embodiments, a two-step cloning system is described, in which the nuclear donor of the first cloning cycle is an adult fibroblast cell and the nuclear donor of the second cloning cycle is a fetal fibroblast harvested from a fetus that arose from the fist cloning cycle.
摘要翻译：公开了选择和克隆方法，其对于所需动物例如牲畜的选择性增殖是有效的。这些方法可用于扩大动物群体，并且特别可用于基于在动物死亡后测量的性状选择的重复动物。某些实施方案包括至少部分地基于可测量的胴体特征来选择期望的牛动物（例如期望的转向）的技术。还描述了涉及克隆的重复（例如，两个）循环的特异性克隆技术，例如核转移克隆。在具体实施方案中，描述了两步克隆系统，其中第一克隆周期的核供体是成体成纤维细胞，第二克隆周期的核供体是从第一次产生的胎儿收获的胎儿成纤维细胞克隆周期。

9. 发明申请

WO01084920A1 THE PRODUCTION OF AGRICULTURAL ANIMALS FROM EMBRYONIC STEM (ES) CELLS 审中-公开
标题翻译：从胚胎干细胞（ES）细胞生产农业动物
公开(公告)号：WO01084920A1
公开(公告)日：2001-11-15
申请号：PCT/US2001/015075
申请日：2001-05-10
IPC分类号： A01K67/027 , C12N15/873 , C12N15/877 , A01K67/00 , C12N15/00
CPC分类号： C12N15/873 , A01K67/0273 , A01K2217/05 , A01K2227/101 , C12N15/8771
摘要： This invention describes a novel method of agricultural animal production that provides animals with optimum growth characteristics in a reliable and cost-effective manner. The invention utilizes a novel combination of Nuclear Transfer (NT) and Embryonic Stem (ES) cell technologies that improves the efficiency of delivering optimized animals and facilitates the introduction of genetic modifications into farm animals.
摘要翻译：本发明描述了一种以可靠和具有成本效益的方式为动物提供最佳生长特性的新型农业动物生产方法。本发明利用核转移（NT）和胚胎干细胞（ES）细胞技术的新型组合，其提高了递送优化动物的效率，并有助于将遗传修饰引入农场动物。

10. 发明申请

WO2015081182A1 ARTIFICIAL OOCYTE ACTIVATION 审中-公开
标题翻译：人工活动
公开(公告)号：WO2015081182A1
公开(公告)日：2015-06-04
申请号：PCT/US2014/067588
申请日：2014-11-26
申请人： THE CURATORS OF THE UNIVERSITY OF MISSOURI
发明人： LEE, Kiho , PRATHER, Randall, S.
IPC分类号： C12N5/075 , C12N15/877
CPC分类号： C12N5/0609 , A01K67/0273 , A01K2227/108 , C12N15/877 , C12N15/8778 , C12N2500/14 , C12N2500/22 , C12N2501/405 , C12N2517/04 , C12N2517/10
摘要： The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn 2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca 2+ in the oocyte prior to reduction of the intracellular Zn 2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.
摘要翻译：本发明提供了通过降低卵母细胞中Zn 2+的细胞内浓度来提高未受精哺乳动物卵母细胞人工活化效率的新方法。本发明的方法可以另外包括在减少细胞内Zn 2+浓度之前增加卵母细胞中Ca 2+的细胞内浓度的前述步骤。本发明还提供了通过所公开的方法活化的未受精的卵母细胞和由所公开的方法激活的未受精的卵母细胞产生的活的哺乳动物。

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