专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1993000355A1 PROTEIN SEQUENCING 审中-公开
标题翻译：蛋白质序列
公开(公告)号：WO1993000355A1
公开(公告)日：1993-01-07
申请号：PCT/US1992005174
申请日：1992-06-12
申请人： PORTON INSTRUMENTS, INC.
发明人： PORTON INSTRUMENTS, INC. , FARNSWORTH, Vincent
IPC分类号： C07K01/12
CPC分类号： G01N33/6824 , C07K1/128 , Y10S530/802
摘要： A method is described for the generation of phenylthiocarbamyl. (PTC) amino acids from phenylthiohydantoin (PTH) or anilinothiozolinone (ATZ) amino acids. The method involves a base-catalyzed ring opening of the PTH or ATZ in the presence of a reducing agent. The method affords an alternative to the established aqueous acid conversion reaction of the Edman degradation in which ATZ and PTC amino acids are converted to the PTH amino acid. A further method is described for the generation of reactive ATZ amino acids from PTH amino acids. These methods facilitate the analysis of protein at low molar amounts by allowing the synthesis of amino acid derivatives which can be analyzed in quantities which are much lower than those required for conventional PTH amino acid analysis.
摘要翻译：描述了用于产生苯基硫代氨基甲酰基的方法。（PTC）氨基酸，苯硫基乙内酰脲（PTH）或苯硫基硫唑酮（ATZ）氨基酸。该方法涉及在还原剂存在下PTH或ATZ的碱催化开环。该方法提供了其中ATZ和PTC氨基酸转化为PTH氨基酸的Edman降解的已建立的含水酸转化反应的替代方案。描述了从PTH氨基酸产生反应性ATZ氨基酸的另一种方法。这些方法通过允许合成可以以比常规PTH氨基酸分析所需量低得多的量分析的氨基酸衍生物来促进低摩尔量的蛋白质的分析。

2. 发明申请

WO1980000351A1 ANALYTICAL FLUOROGENIC SUBSTRATES FOR PROTEOLYTIC ENZYMES 审中-公开
标题翻译：分解性荧光基质用于蛋白酶
公开(公告)号：WO1980000351A1
公开(公告)日：1980-03-06
申请号：PCT/US1979000421
申请日：1979-06-15
申请人： AMERICAN HOSPITAL SUPPLY CORP
发明人： AMERICAN HOSPITAL SUPPLY CORP , MITCHELL G , SMITH R , HUDSON P , POCHRON S , HUSEBY R , GARGIULO R
IPC分类号： C12Q01/36
CPC分类号： C07K5/0823 , C07K5/0804 , C07K5/0812 , C07K5/0819 , C07K5/1013 , C12Q1/37 , C12Q1/56 , C12Q2337/10 , C12Q2337/30 , G01N2333/968 , G01N2333/974 , G01N2400/40 , Y10S530/802 , Y10S930/28
摘要： Fluorogenic substrates for proteolytic enzymes having the formula: (FORMULA) or acid salts thereof wherein: R1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxyserine; R2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid; R3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane; R4 is methyl, 4-aminobutane, or 3-quanidylpropane; R5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound. The enzymes, when reacting with the substrate, remove the fluorogenic group R5 producing an increase in its fluorescence. The increase of the fluorescence is an indication of the activity of the enzyme present. Specific enzymes thusly detectable include plasmin, thrombin, the factors Xa, XIa, & XIIa, kallikrein, trypsin, elastase, urokinase, and cathepsin B1.

3. 发明申请

WO98055870A1 DETECTION OF BIOLOGICALLY ACTIVE MOLECULES BY USE OF PRE-ACTIVATED CELL-BASED BIOSENSORS IN LIQUID-BASED SEPARATION SYSTEMS 审中-公开
标题翻译：通过在基于液体的分离系统中使用基于预激活细胞的生物传感器来检测生物活性分子
公开(公告)号：WO98055870A1
公开(公告)日：1998-12-10
申请号：PCT/SE1998/001058
申请日：1998-06-03
IPC分类号： G01N27/447 , G01N33/543 , G01N33/566 , G01N33/68 , G01N33/567 , C12M1/40 , C12Q1/02 , G01N33/487
CPC分类号： G01N33/5438 , G01N27/447 , G01N33/566 , G01N33/6803 , G01N2333/70571 , Y10S530/802
摘要： The present invention relates to a method for detection of receptor antagonists comprising the following steps: (I) a sample containing the receptor antagonist is fractionated by use of a liquid-based separation means, preferably capillary electrophoresis; (II) a fraction containing the receptor antagonist is fed directly to a biosensor (9), preferably a living cell, activated by a receptor agonist and, as a result of this activation, is generating a measurable response, said agonist being fed to the biosensor through the liquid-based separation means together with the antagonist; and (III) the change of the response resulting from deactivation of the receptor agonist-activated biosensor by the receptor antagonist or modulator is measured, preferably by means of a patch clamp electrode (8). It is further possible to resensitise the biosensor desensitised as above by use of interlocked pulsed superfusion of the biosensor. The present invention also relates to an apparatus usable for practising the above mentioned method.
摘要翻译：本发明涉及一种用于检测受体拮抗剂的方法，包括以下步骤：（I）含有受体拮抗剂的样品通过使用液体分离方法，优选毛细管电泳分级分离; （II）将含有受体拮抗剂的级分直接供给到由受体激动剂活化的生物传感器（9），优选活细胞，并且由于该活化，产生可测量的反应，所述激动剂被供给至通过液体分离装置与拮抗剂一起生物传感器; 和（III）优选通过膜片钳电极（8）测量由受体拮抗剂或调节剂由受体激动剂活化的生物传感器失活引起的反应的变化。还可以通过使用生物传感器的互锁的脉冲灌注对如上所述的生物传感器进行再敏感。本发明还涉及可用于实施上述方法的装置。

4. 发明申请

WO1989000291A1 FLUORESCEIN-CONJUGATED PROTEINS WITH ENHANCED FLUORESCENCE 审中-公开
标题翻译：具有增强荧光的荧光素结合蛋白
公开(公告)号：WO1989000291A1
公开(公告)日：1989-01-12
申请号：PCT/US1988002240
申请日：1988-07-01
申请人： SCLAVO INC., WEST COAST
发明人： SCLAVO INC., WEST COAST , RONALD, Robert, C. , NGUYEN, Phuc, Huu , ROWLEY, Gerald, L.
IPC分类号： G01N33/53
CPC分类号： C07D311/82 , C09B11/08 , C09B49/126 , G01N33/533 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10S530/802 , Y10S530/866
摘要： A method for conducting assays for an analyte, usually in a biological sample, utilizes reagents which contain biological ligands linked to fluorescein or a derivative of fluorescein through a linkage to the fluorescein nucleus selected from acetamido and thioacetamido. In these reagents, the fluorescence efficiency is improved over that obtained in the commonly used FITC-labeled reagents.

5. 发明申请

WO1982000641A1 PEPTIDE SUBSTRATES FOR DETERMINATION OF PROTEASE ACTIVITY 审中-公开
标题翻译：用于确定蛋白酶活性的肽基质
公开(公告)号：WO1982000641A1
公开(公告)日：1982-03-04
申请号：PCT/SE1981000235
申请日：1981-08-24
申请人： KABIVITRUM AB
发明人： KABIVITRUM AB , AURELL L , SIMONSSON L , CLAESON K , ARIELLY S
IPC分类号： C07C103/52
CPC分类号： C12Q1/37 , C07D237/32 , C07K5/08 , C12Q2337/00 , Y10S530/802 , Y10S930/28
摘要： Nouveaux derives peptidiques qui sont des derives acyles de luminol(5-amino-2,3-dihydro-1,4-phthalazinedione) ou le reste acyle est un acide amine ou une sequence d'acide amine avec 2 a 4 residus d'acides amines couples avec une liaison amide, et ou le groupe (Alpha)-amino est soit libre soit acyle, leurs procedes de preparation et un procede de diagnostic en laboratoire de proteases utilisant les derives peptidiques. Les derives sont destines a la determination quantitative de proteases ou l'activite des proteases par liberation de luminol ou d'isoluminol qui a leur etat libre peuvent etre amenes a s'illuminer de maniere intense, mais perdent considerablement en luminescence lorsqu'ils ont une liaison amide sur une sequence peptidique.

6. 发明申请

WO1987000196A1 PROTECTION OF PROTEINS AND THE LIKE 审中-公开
标题翻译：保护蛋白质等
公开(公告)号：WO1987000196A1
公开(公告)日：1987-01-15
申请号：PCT/GB1986000396
申请日：1986-07-09
申请人： QUADRANT BIORESOURCES LIMITED , ROSER, Bruce, Joseph
发明人： QUADRANT BIORESOURCES LIMITED
IPC分类号： C12N09/96
CPC分类号： A61K39/00 , A61K35/16 , A61K47/26 , C07K1/00 , C07K16/00 , C12N9/96 , G01N33/50 , G01N33/531 , Y10S530/802 , Y10S530/83
摘要： PCT No. PCT/GB86/00396 Sec. 371 Date May 7, 1987 Sec. 102(e) Date May 7, 1987 PCT Filed Jul. 9, 1986 PCT Pub. No. WO87/00196 PCT Pub. Date Jan. 15, 1987.Sensitive proteins and other macromolecules, such as enzymes, antibodies, antigens, serum complement, fluorescent proteins, vaccine components, polysaccharides such as agarose etc, can be preserved by drying at ambient temperature and at atmospheric pressure in the presence of trehalose. A porous matrix impregnated with trehalose is provided as a receiver for a blood or other liquid sample to be dried.

7. 发明申请

WO1986007061A1 WATER SOLUBLE XANTHYLIUM DERIVATIVE SUBSTRATES 审中-公开
标题翻译：水溶性XANTHYLIUM衍生基板
公开(公告)号：WO1986007061A1
公开(公告)日：1986-12-04
申请号：PCT/US1986001136
申请日：1986-05-27
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , MITCHELL, Gary, A. , JAFFE, Gerald, E. , SOLORZANO, Marilyn, M.
IPC分类号： C07K01/02
CPC分类号： C07K5/0821 , C07K5/0806 , C12Q1/37 , C12Q1/56 , C12Q2337/40 , G01N2333/8128 , G01N2333/974 , G01N2400/40 , Y10S530/802 , Y10S930/28
摘要： Water soluble xanthylium derivative substrates of rhodamine (110) and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation.

8. 发明申请

WO1986001604A1 LABELLED AND LABELLABLE REAGENTS FOR FLUOROMETRIC ASSAYS 审中-公开
标题翻译：用于荧光测定的标签和可溶性试剂
公开(公告)号：WO1986001604A1
公开(公告)日：1986-03-13
申请号：PCT/GB1985000377
申请日：1985-08-22
申请人： EKINS, Roger, Philip , DAKUBU, Salifu
发明人： EKINS, Roger, Philip
IPC分类号： G01N33/533
CPC分类号： G01N33/533 , G01N33/582 , Y10S436/805 , Y10S436/808 , Y10S530/802
摘要： Labellable reagents for fluorometric assays comprise a cyclic condensation product of a beta -diketone, an aldehyde and an NH2 -bearing macromolecule, for example an antigen or antibody or a substance having an active group to which an antibody or antigen is linked. The reagents can be chelated to lanthanide metal ions such as Eu (III) and Tb (III) to form fluorescing complexes which can be used as labelled reagents for fluorometric assay of organic substances, for example antigens, antibodies and other substances occurring in body fluids.
摘要翻译：用于荧光测定的可溶性试剂包括β-二酮，醛和NH 2担载大分子的环状缩合产物，例如抗原或抗体或具有抗体或抗原所连接的活性基团的物质。这些试剂可以螯合镧系元素金属离子如Eu（III）和Tb（III）以形成荧光复合物，其可以用作有机物质的荧光测定的标记试剂，例如体液中发生的抗原，抗体和其它物质。

9. 发明申请

WO2006009838A3 MYCOBACTERIUM TUBERCULOSIS EPITOPES AND METHODS OF USE THEREOF 审中-公开
标题翻译：结核分枝杆菌表位及其使用方法
公开(公告)号：WO2006009838A3
公开(公告)日：2006-04-20
申请号：PCT/US2005021451
申请日：2005-06-17
申请人： BECKMAN COULTER INC , MAEURER MARKUS
发明人： MAEURER MARKUS
IPC分类号： A61K38/04 , A61K38/00
CPC分类号： G01N33/56977 , C07K14/35 , G01N33/5695 , G01N2469/20 , G01N2800/52 , Y10S530/802 , Y10S530/806 , Y10S530/825
摘要： The invention provides novel Class I HLA-A2 and Class II HLA-DR4-restricted epitopes and methods for their use in detecting T-cells in peripheral blood specific for infection or latency of mycobacterial infection, including M tuberculosis and M leprae as others.. For example, methods for diagnosing the presence of infection or exposure by M. tuberculosis utilize multimers of HLA monomers or modified monomers having a bound HLA-binding peptide to perform high throughput screening of patient PBLs. The methods can be used for monitoring the success of anti mycobacterial treatment in patients and to screen. vaccines and drugs for effectiveness in treating or preventing exposure, infection and latency of mycobacteria in humans.
摘要翻译：本发明提供了新的I类HLA-A2和II类HLA-DR4限制性表位以及它们用于检测对感染或潜伏分枝杆菌感染（包括结核分枝杆菌和麻风杆菌等）特异性的外周血中的T细胞的方法。例如，用于诊断结核分枝杆菌感染或暴露的存在的方法利用HLA单体的多聚体或具有结合的HLA结合肽的修饰单体来进行患者PBL的高通量筛选。该方法可用于监测患者中抗分枝杆菌治疗的成功并筛查。疫苗和药物，用于治疗或预防人类中分枝杆菌的接触，感染和潜伏期。

10. 发明申请

WO2006017196A1 STABLE CYANINE DYE PHOSPHORAMIDITES 审中-公开
标题翻译：稳定的氰基染料磷光体
公开(公告)号：WO2006017196A1
公开(公告)日：2006-02-16
申请号：PCT/US2005/024383
申请日：2005-07-08
申请人： BECKMAN COULTER, INC. , MICHAEL, Maged, A. , FAROOQUI, Firdous , REDDY, M.P. , LI, Hong
发明人： MICHAEL, Maged, A. , FAROOQUI, Firdous , REDDY, M.P. , LI, Hong
IPC分类号： C07H21/00 , C09B23/00 , G01N33/533 , G01N33/58 , C12Q1/68
CPC分类号： C07H21/00 , C09B23/0025 , C09B23/0066 , Y02P20/55 , Y10S530/802
摘要： This invention provides dye phosphoramidites, particularly phosphoramidites of substituted cyclic bridged cyanine and related dyes, of the general formula: In this formula, each dotted line represents carbon atoms necessary to form a fused substituted or unsubstituted aromatic ring; m is an integer from 1 to 18; Y and Z are independently selected from the group consisting of S, O, N, CH 2 and C(CH 3 ) 2 ; R 1 is an alkyl; (PAM) is phosphoramidite group; X is a negative ion; and Q is L-W, wherein L is a conjugated cyclic moiety and W is OR 2 , wherein R 2 is second alkyl. Methods of making and using the dye phosphoramidites are also provided.
摘要翻译：本发明提供了以下通式的染料亚磷酰胺，特别是取代的环状桥连花青及相关染料的亚磷酰胺，其中，虚线表示形成稠合取代或未取代的芳环所需的碳原子; m为1〜18的整数， Y和Z独立地选自S，O，N，CH

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式