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1. 发明申请

WO2006017196A1 STABLE CYANINE DYE PHOSPHORAMIDITES 审中-公开
标题翻译：稳定的氰基染料磷光体
公开(公告)号：WO2006017196A1
公开(公告)日：2006-02-16
申请号：PCT/US2005/024383
申请日：2005-07-08
申请人： BECKMAN COULTER, INC. , MICHAEL, Maged, A. , FAROOQUI, Firdous , REDDY, M.P. , LI, Hong
发明人： MICHAEL, Maged, A. , FAROOQUI, Firdous , REDDY, M.P. , LI, Hong
IPC分类号： C07H21/00 , C09B23/00 , G01N33/533 , G01N33/58 , C12Q1/68
CPC分类号： C07H21/00 , C09B23/0025 , C09B23/0066 , Y02P20/55 , Y10S530/802
摘要： This invention provides dye phosphoramidites, particularly phosphoramidites of substituted cyclic bridged cyanine and related dyes, of the general formula: In this formula, each dotted line represents carbon atoms necessary to form a fused substituted or unsubstituted aromatic ring; m is an integer from 1 to 18; Y and Z are independently selected from the group consisting of S, O, N, CH 2 and C(CH 3 ) 2 ; R 1 is an alkyl; (PAM) is phosphoramidite group; X is a negative ion; and Q is L-W, wherein L is a conjugated cyclic moiety and W is OR 2 , wherein R 2 is second alkyl. Methods of making and using the dye phosphoramidites are also provided.
摘要翻译：本发明提供了以下通式的染料亚磷酰胺，特别是取代的环状桥连花青及相关染料的亚磷酰胺，其中，虚线表示形成稠合取代或未取代的芳环所需的碳原子; m为1〜18的整数， Y和Z独立地选自S，O，N，CH

2. 发明申请

WO2004104230A1 REDUCING MICROARRAY VARIATION WITH INTERNAL REFERENCE SPOTS 审中-公开
标题翻译：减少具有内部参考点的微观变化
公开(公告)号：WO2004104230A1
公开(公告)日：2004-12-02
申请号：PCT/US2004/015209
申请日：2004-05-14
申请人： BECKMAN COULTER, INC. , REDDY, Parameswara, Meda , BRILLHART, Kurt , FAROOQUI, Firdous , KEYS, Daniel, A.
发明人： REDDY, Parameswara, Meda , BRILLHART, Kurt , FAROOQUI, Firdous , KEYS, Daniel, A.
IPC分类号： C12Q1/68
CPC分类号： C40B30/04 , C12Q1/6837 , G01N33/54373 , G01N33/54393 , C12Q2545/101
摘要： The present invention provides a system using internal reference spots to reduce microarray assay variation. A microarray device for quantifying a plurality of target ligands in a sample includes a solid support having a plurality of test areas; a plurality of different target capture ligands immobilized onto the test areas; and at least one reference capture ligand immobilized onto the test areas. The reference capture ligand captures a reference ligand not normally present in the sample.
摘要翻译：本发明提供了一种使用内部参考斑点以减少微阵列测定变化的系统。用于量化样品中多个靶配体的微阵列装置包括具有多个测试区域的固体支持物; 固定在测试区域上的多个不同靶捕获配体; 和固定在测试区域上的至少一个参考捕获配体。参考捕获配体捕获样品中通常不存在的参考配体。

3. 发明申请

WO2005118855A1 ASSAY SYSTEM USING LABELED OLIGONUCLEOTIDES 审中-公开
标题翻译：使用标记的寡核苷酸的测定系统
公开(公告)号：WO2005118855A1
公开(公告)日：2005-12-15
申请号：PCT/US2004/015982
申请日：2004-05-20
申请人： BECKMAN COULTER, INC. , REDDY, Parameswara, M. , KEYS, Daniel, A. , FAROOQUI, Firdous
发明人： REDDY, Parameswara, M. , KEYS, Daniel, A. , FAROOQUI, Firdous
IPC分类号： C12Q1/68
CPC分类号： C07H21/04 , C12Q1/6804 , C12Q1/6816 , C12Q1/6834 , C12Q2565/519 , C12Q2563/179 , C12Q2563/131
摘要： The present invention provides a useful system for assays that comprises a solid support, a plurality of capture oligonucleotides immobilized onto the solid support, and complementary oligonucleotides attached to capture ligands. A detectable label can be directly attached to the capture oligonucleotides or the complementary oligonucleotides. The labeled oligonucleotides can be detected, and used to determine the quality of the assay. A labeled detector ligand corresponding to a target ligand can also be independently detected apart from the labeled oligonucleotide.
摘要翻译：本发明提供了用于包含固体支持物，固定在固体支持物上的多个捕获寡核苷酸和与捕获配体连接的互补寡核苷酸的测定的有用系统。可检测标记可以直接连接到捕获寡核苷酸或互补寡核苷酸。可以检测标记的寡核苷酸，并用于确定测定的质量。对应于靶配体的标记的检测体配体也可以与标记的寡核苷酸分开检测。

4. 发明申请

WO2004094668A2 OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS 审中-公开
标题翻译：用于多重绑定测定的寡核苷酸对
公开(公告)号：WO2004094668A2
公开(公告)日：2004-11-04
申请号：PCT/US2004/011885
申请日：2004-04-16
申请人： BECKMAN COULTER, INC. , KEYS, Daniel, A. , REDDY, Parameswara, Meda , BODNAR, Jackie, S. , FAROOQUI, Firdous
发明人： KEYS, Daniel, A. , REDDY, Parameswara, Meda , BODNAR, Jackie, S. , FAROOQUI, Firdous
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6837 , C12Q2565/501 , C12Q2537/125
摘要： The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

5. 发明申请

WO2003035830A2 EFFICIENT SYNTHESIS OF PROTEIN-OLIGONUCLEOTIDE CONJUGATES 审中-公开
标题翻译：高效合成蛋白质寡聚核苷酸
公开(公告)号：WO2003035830A2
公开(公告)日：2003-05-01
申请号：PCT/US2002/032317
申请日：2002-10-11
申请人： BECKMAN-COULTER, INC.
发明人： FAROOQUI, Firdous , REDDY, Parameswara, M.
IPC分类号： C12N
CPC分类号： C07H21/00 , A61K47/6807
摘要： The present invention relates to an improved method for forming a protein-oligonucleotide conjugate. The method is particularly amenable for forming antibody-oligonucleotide conjugates. The invention further concerns the conjugate molecules produced using such improved methods.
摘要翻译：本发明涉及形成蛋白质 - 寡核苷酸缀合物的改进方法。该方法特别适用于形成抗体 - 寡核苷酸缀合物。本发明还涉及使用这种改进方法产生的缀合物分子。

6. 发明申请

WO2003036257A3 IMMOBILIZING BIOLOGICAL MOLECULES 审中-公开
公开(公告)号：WO2003036257A3
公开(公告)日：2003-05-01
申请号：PCT/US2002/033059
申请日：2002-10-17
申请人： BECKMAN COULTER, INC.
发明人： REDDY, M. Parameswara , FAROOQUI, Firdous
IPC分类号： G01N33/543
摘要： This invention provides a system for immobilizing biological molecules onto a solid support having an available amino group that uses two steps. In a first step, a nucleophilic substitution reaction occurs so that the available amino group displaces a first leaving group of an activating compound to form an activated support. In a second step, the activated support reacts with biological molecules resulting in a composition of the formula: (I) wherein S is a solid support, preferably X is selected from the group consisting of (i), (ii), (iii), (iv), wherein R is selected from the group consisting of alkyl, aryl, and OR 1 about 18 carbon atoms, wherein R 1 is selected from the group consisting of alkyl and aryl having no greater than about 18 carbon atoms, wherein X 1 is selected from the group consisting of NH, oxygen, and sulfur, and wherein B is a biological molecule.

7. 发明申请

WO2003036257A2 IMMOBILIZING BIOLOGICAL MOLECULES 审中-公开
标题翻译：保持生物分子
公开(公告)号：WO2003036257A2
公开(公告)日：2003-05-01
申请号：PCT/US2002/033059
申请日：2002-10-17
申请人： BECKMAN COULTER, INC.
发明人： REDDY, M. Parameswara , FAROOQUI, Firdous
IPC分类号： G01N
CPC分类号： G01N33/54353
摘要： This invention provides a system for immobilizing biological molecules onto a solid support having an available amino group that uses two steps. In a first step, a nucleophilic substitution reaction occurs so that the available amino group displaces a first leaving group of an activating compound to form an activated support. In a second step, the activated support reacts with biological molecules resulting in a composition of the formula: (I) wherein S is a solid support, preferably X is selected from the group consisting of (i), (ii), (iii), (iv), wherein R is selected from the group consisting of alkyl, aryl, and OR 1 about 18 carbon atoms, wherein R 1 is selected from the group consisting of alkyl and aryl having no greater than about 18 carbon atoms, wherein X 1 is selected from the group consisting of NH, oxygen, and sulfur, and wherein B is a biological molecule.
摘要翻译：本发明提供了一种用于将生物分子固定在具有使用两个步骤的可用氨基的固体支持物上的系统。在第一步中，发生亲核取代反应，使得可用的氨基取代活化化合物的第一离去基团以形成活化的载体。在第二步中，活化的载体与生物分子反应，产生下式的组合物：（I）其中S是固体支持物，优选X选自（i），（ii），（iii），（iv）其中R选自烷基，芳基和OR 18约18个碳原子，其中R 1选自不大于约18碳的烷基和芳基原子，其中X 1选自NH，氧和硫，并且其中B是生物分子。

8. 发明申请

WO2003035830A3 EFFICIENT SYNTHESIS OF PROTEIN-OLIGONUCLEOTIDE CONJUGATES 审中-公开
公开(公告)号：WO2003035830A3
公开(公告)日：2003-05-01
申请号：PCT/US2002/032317
申请日：2002-10-11
申请人： BECKMAN-COULTER, INC.
发明人： FAROOQUI, Firdous , REDDY, Parameswara, M.
IPC分类号： C12Q1/68
摘要： The present invention relates to an improved method for forming a protein-oligonucleotide conjugate. The method is particularly amenable for forming antibody-oligonucleotide conjugates. The invention further concerns the conjugate molecules produced using such improved methods.

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