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1. 发明申请

WO1990013013A1 METHOD AND APPARATUS FOR SCREENING CELLS OR FORMED BODIES WITH POPULATIONS EXPRESSING SELECTED CHARACTERISTICS UTILIZING AT LEAST ONE SENSING PARAMETER 审中-公开
标题翻译：用于筛选具有使用至少一个传感参数的选择特征的人群的细胞或形成体的方法和装置
公开(公告)号：WO1990013013A1
公开(公告)日：1990-11-01
申请号：PCT/US1990001880
申请日：1990-04-06
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , RUSSELL, Thomas , HAJEK, Constance, Mary , RODRIGUEZ, Carlos, M. , COULTER, Wallace, H.
IPC分类号： G01N01/10
CPC分类号： G01N1/28 , G01N15/10 , G01N33/54326 , G01N33/56972 , G01N2015/1062 , G01N2015/1081 , G01N2015/1486 , G01N2015/149
摘要： A method and apparatus (500) for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell (WBC) population and at least one subset thereof of a whole blood sample (502) or portion thereof. A volume of a biological medium containing the WBC's is prepared and at least one reactant (506) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. A multipart blood cell analysis (512) is obtained with a single sensing parameter by depleting at least one WBC subset population. The percentage of a desired WBC population subset or the overlapping of WBC subset populations also can be obtained by subtracting one or more obscuring WBC subset populations.
摘要翻译：一种用于自动和快速地，检索，计数和/或分析至少一个选定的细胞群或成体体（例如白细胞（WBC）群体）及其全血的至少一个子集的方法和装置（500）样品（502）或其部分。制备含有WBC的生物培养基的体积，并将至少一种特定的或优先选择的至少一些选定的生物细胞的反应物（506）引入其中并在短时间内快速混合。通过消耗至少一个WBC子群体，通过单个感测参数获得多部分血细胞分析（512）。通过减去一个或多个模糊的WBC子集群，可以获得期望的WBC群体子集的百分比或WBC子群体的重叠。

2. 发明申请

WO1988007199A1 AUTOMATED ANALYZER AND METHOD FOR SCREENING CELLS OR FORMED BODIES FOR ENUMERATION OF POPULATIONS EXPRESSING SELECTED CHARACTERISTICS 审中-公开
标题翻译：自动分析仪和筛选细胞或形成体系的方法，用于表达选择特征的人群
公开(公告)号：WO1988007199A1
公开(公告)日：1988-09-22
申请号：PCT/US1988000960
申请日：1988-03-11
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , KORTRIGHT, Kenneth, H. , COULTER, Wallace, H. , RODRIGUEZ, Carlos , RUSSELL, Thomas , PAUL, Ronald
IPC分类号： G01N33/53
CPC分类号： G01N33/56972 , G01N15/12 , G01N15/14 , G01N33/5094 , G01N33/5302 , G01N33/54326 , G01N35/0098 , G01N2015/1037 , G01N2015/1062 , G01N2015/1081
摘要： A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample (60) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (66, 74, 144, 132) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse (66) or a monoclonal antibody bound to microspheres (132, 144), which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
摘要翻译：一种用于自动并快速地检索，计数和/或分析全血样品（60）或其部分的至少一个所选择的白细胞群体和/或其子集的方法和装置。制备一定体积的含有白细胞的生物培养基，至少将一些选定的生物细胞特异性或优先选择的至少一种反应物（66,74,144,132）引入其中并在短时间内快速混合。可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析以获得所需的白细胞群体分析。生物样品可以是全血样品，反应物可以包括裂解物（66）或与微球结合的单克隆抗体（132,144），其将结合特定的细胞或裂解和微球的组合与抗体结合。微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。

3. 发明申请

WO1988006918A1 METHOD AND APPARATUS FOR RAPID MIXING OF SMALL VOLUMES FOR ENHANCING BIOLOGICAL REACTIONS 审中-公开
标题翻译：用于增强生物反应的小容量快速混合的方法和装置
公开(公告)号：WO1988006918A1
公开(公告)日：1988-09-22
申请号：PCT/US1988000969
申请日：1988-03-11
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , COULTER, Wallace, H. , HOLLINGER, John, David , RUSSELL, Thomas , RODRIGUEZ, Carlos , PAUL, Ronald , KORTRIGHT, Kenneth, H.
IPC分类号： B01L03/00
CPC分类号： G01N33/5005 , G01N33/56966 , G01N33/56972
摘要： A method and apparatus (10, 24, 46, 76, 90, 102) for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.
摘要翻译：一种用于加速至少一种确定性生物反应的方法和装置（10,24,46,76,90,102），包括增加由其制备的测定精度。该反应涉及选择的活体生物细胞，它们以小的样品体积制备，并且与具有抗体特异性至少与结合于其上的特定细胞的微球快速混合。微球可以是磁性的，并且结合的细胞可以被磁性去除以分析剩余的血细胞群体。微球可以顺序或同时引入。

4. 发明申请

WO1988003267A1 PARTICLE ANALYZER FOR MEASURING THE RESISTANCE AND REACTANCE OF A PARTICLE 审中-公开
标题翻译：用于测量颗粒的电阻和反应的颗粒分析仪
公开(公告)号：WO1988003267A1
公开(公告)日：1988-05-05
申请号：PCT/US1987002744
申请日：1987-10-20
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , COULTER, Wallace, H. , RODRIGUEZ, Carlos, M.
IPC分类号： G01N27/00
CPC分类号： G01N15/1227 , G01N2015/129
摘要： A circuit (10) provides data needed to measure the electrical opacity of a particle (20), such as a blood cell passing through a Coulter type transducer (12). The circuit (10) includes a current source (22) for providing a conventional d.c. current through the sensing aperture (16) of the transducer (12), and an oscillator (24) for providing a high frequency current through the aperture (16). The oscillator (24) includes an active device (32) and a resonant circuit (34), and the aperture (16) is coupled in parallel with the resonant circuit (34). As a particle (20) passes through the aperture (16), the resistance of the aperture (16) increases, which increases the Q of the oscillator (24), whereby the oscillator (24) output signal (Fig. 3) is amplitude modulated in accordance with the increased Q. Multiple oscillators (F1, F2 ... FN) of different frequencies can be coupled in parallel with the transducer (12) through a coupling cirucit (54, 77 and 79), which connects each oscillator (F1, F2 ... FN) through a low impedance path and isolates each oscillater (F1, F2 ... FN) from signals having frequencies of the other oscillators.
摘要翻译：电路（10）提供测量粒子（20）的电不透性所需的数据，例如通过库尔特型换能器（12）的血细胞。电路（10）包括用于提供常规直流电流的电流源（22）。通过传感器（12）的检测孔（16）的电流以及用于提供高频电流通过孔（16）的振荡器（24）。振荡器（24）包括有源器件（32）和谐振电路（34），并且孔（16）与谐振电路（34）并联耦合。当颗粒（20）穿过孔（16）时，孔径（16）的电阻增加，这增加了振荡器（24）的Q，由此振荡器（24）的输出信号（图3）是振幅根据增加的Q进行调制。不同频率的多个振荡器（F1，F2 ... FN）可以通过连接每个振荡器（54,77和79）的耦合电路（54,77和79）与换能器（12）并联耦合， F1，F2 ... FN）通过低阻抗路径隔离每个振荡器（F1，F2 ... FN）与具有其他振荡器频率的信号。

5. 发明申请

WO1988001234A1 AUTOMATIC PRINTING APPARATUS UTILIZING TURNAROUND DOCUMENT 审中-公开
标题翻译：自动打印设备利用文件
公开(公告)号：WO1988001234A1
公开(公告)日：1988-02-25
申请号：PCT/US1987002058
申请日：1987-08-19
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , GARCIA DE OSUNA, Ignacio, B. , PARKER, Bernard , WINTER, Warren, C. , SIOREK, Terry, Lee , ZORRILLA, Rafael, E. , DOTY, Edward, Neal , MATSUDA, Hari
IPC分类号： B41J03/50
CPC分类号： G06K17/00 , B41J3/50 , G06K2017/0041 , G06K2017/0077 , G06K2017/009
摘要： Automatic printing apparatus (10) wherein an individual sheet item (26), initially bearing machine and human readable data, is utilized initially to identify and accumulate individual original data and thereafter is reemployed as a final repository for additional data from an external source of data. The original data is compared with the additional data to determine correspondence or lack thereof. Correspondence of the data produces a control output (276) which causes the apparatus (10) to print the additional data on the item (26) and automatically to select an accept item receiving receptacle (22). Lack of correspondence between the original data and the additional data produces a control output automatically to select a reject item receiving receptacle (24).

6. 发明申请

WO1984004969A1 REAGENT FOR COMBINED DILUTING AND LYSING WHOLE BLOOD 审中-公开
标题翻译：联合给药和全身麻醉的试剂
公开(公告)号：WO1984004969A1
公开(公告)日：1984-12-20
申请号：PCT/US1984000768
申请日：1984-05-18
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , LARSEN, Fred, L.
IPC分类号： G01N33/48
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/101666 , Y10T436/105831 , Y10T436/107497 , Y10T436/108331
摘要： An improved reagent provides combined diluting and lysing of whole blood for use in instruments performing electronic blood cell counting and hemoglobin determination. The reagent prevents platelet aggregation and improves the accuracy of the white blood cell count. The reagent comprises a quaternary ammonium detergent and a salt of an anion selected from the group consisting of sulfate, carbonate, formate, and acetate.

7. 发明申请

WO1990007259A2 METHOD AND APPARATUS FOR SCREENING CELLS OR FORMED BODIES WITH POPULATIONS EXPRESSING SELECTED CHARACTERISTICS 审中-公开
标题翻译：用于筛选表达选定特征的人群的细胞或形成体的方法和装置
公开(公告)号：WO1990007259A2
公开(公告)日：1990-07-12
申请号：PCT/US1989005548
申请日：1989-12-13
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , KORTRIGHT, Kenneth, H. , COULTER, Wallace, H. , RODRIGUEZ, Carlos , RUSSELL, Thomas , PAUL, Ronald
IPC分类号： G01N33/53 , A61K49/00 , C12N5/02 , G01N15/00 , G01N15/10 , G01N15/12 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/577
CPC分类号： G01N15/12 , G01N33/56972 , G01N2015/008 , G01N2015/1062 , G01N2015/1081
摘要： A method and apparatus (320, 340, 360) for automatically and rapidly retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample (322, 342) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (326, 346) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed (384) for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed (332, 352, 416, 422) in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
摘要翻译：一种用于自动和快速地检索，计数和/或分析至少一个选定的细胞群体或成体体（例如白细胞群体及其全血的至少一个子集）的方法和装置（320,340,360）样品（322,342）或其部分。制备一定体积的含有白细胞的生物培养基，并将至少一种至少特定或优先选择的一些选择的生物细胞的反应物（326,346）引入其中并在短时间内快速混合（384）。可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析（332,352,416,422）以获得所需的白细胞群体和子集分析。生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特异性细胞结合，或与结合抗体的溶血和微球体的组合。微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。

8. 发明申请

WO1988007198A1 MULTI-PART DIFFERENTIAL ANALYZING APPARATUS UTILIZING LIGHT SCATTER TECHNIQUES 审中-公开
标题翻译：多分辨率分析仪利用光散射技术
公开(公告)号：WO1988007198A1
公开(公告)日：1988-09-22
申请号：PCT/US1988000753
申请日：1988-03-14
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , RODRIGUEZ, Carlos, M. , COULTER, Wallace, H.
IPC分类号： G01N33/48
CPC分类号： G01N15/1459 , G01N1/38 , G01N21/53 , G01N2015/008 , G01N2015/1037 , G01N2015/1062 , G01N2015/129 , G01N2015/1413 , G01N2015/1477 , G01N2021/4711
摘要： A particle analyzing method and apparatus for white blood cell (leukocyte) differentiation by optical and electronic measurements, without the use of dyes, on a flowing stream of leukocytes. A hydrodynamically focused stream of the leukocytes, in native condition, is passed into and through a beam (20) of radiant energy in a flow cell (10), whereby the beam is uniquely scattered by different ones of the subpopulations of the leukocytes. The scattered light impinges upon light collection means (24) disposed adjacent the flow cell. For eosinophil detection, the scattered light is collected at angular positions from about 10 DEG to 70 DEG relative to the axis (26) of the beam (20). Other of the leukocyte subpopulations are differentiated relative to the eosinophils and each other by optical and/or electronic measurements, especially including the Coulter Principle of particle analysis.
摘要翻译：用于通过光学和电子测量的白细胞（白细胞）分化的粒子分析方法和装置，不使用染料，在白细胞的流动流上。在天然状态下，白细胞的流体动力学聚焦的流进入并通过流动池（10）中的辐射能束（20），由此该束被白细胞的不同亚群独特地散射。散射的光照射在邻近流动池设置的光收集装置（24）上。对于嗜酸性粒细胞检测，散射光在相对于光束（20）的轴线（26）约10°至70°的角位置收集。白细胞亚群中的其他白细胞相对于嗜酸性粒细胞和通过光学和/或电子测量彼此分化，特别是包括库尔特粒子分析原理。

9. 发明申请

WO1988002486A1 DEBRIS DETECTOR 审中-公开
标题翻译： DEBRIS检测器
公开(公告)号：WO1988002486A1
公开(公告)日：1988-04-07
申请号：PCT/US1987002537
申请日：1987-10-02
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , ROOS, Ermi , COULTER, Wallace, H.
IPC分类号： G01N27/00
CPC分类号： G01N15/1227 , G01N2015/1281
摘要： In a debris detector for a particle counter (10), a particle (18), or transient debris passing through the sensing orifice (16) causes a voltage pulse occurs (fig. 3A). When debris lodges in or against the orifice (16) of the particle detector (10), a d.c. voltage shift occurs. The voltage at the orifice (16) is capacitively (34) coupled to an amplifier (36) and the output of the amplifier (36) is provided to an integrator (40). The integrated voltage (Fig. 3C) is applied to a comparator (42) to provide an output signal (Fig. 3D) whenever the integrated voltage (Fig. 3C) exceeds a reference voltage (Vref). The comparator (42) output signal (Fig. 3D) is provided to a delay circuit (44, 46) having a delay related to the time required for a particle (18) to pass through the orifice (16). If the comparator (42) signal (Fig. 3D) remains after the delay time, a debris alarm signal (Fig. 3E) is provided.
摘要翻译：在用于颗粒计数器（10）的碎屑检测器中，通过感测孔（16）的颗粒（18）或瞬态碎片引起电压脉冲（图3A）。当碎屑落入或抵靠颗粒检测器（10）的孔口（16）时，发生电压偏移。孔16处的电压是电容性地耦合到放大器（36），并且放大器（36）的输出被提供给积分器（40）。每当集成电压（图3C）超过参考电压（Vref）时，集成电压（图3C）被施加到比较器（42）以提供输出信号（图3D）。将比较器（42）输出信号（图3D）提供给具有与颗粒（18）通过孔口（16）所需的时间相关的延迟的延迟电路（44,46）。如果在延迟时间之后比较器（42）信号（图3D）保留，则提供碎片报警信号（图3E）。

10. 发明申请

WO1987001607A1 APPARATUS FOR ACOUSTICALLY REMOVING PARTICLES FROM A MAGNETIC SEPARATION MATRIX 审中-公开
标题翻译：用于从磁分离矩阵中主动去除颗粒的装置
公开(公告)号：WO1987001607A1
公开(公告)日：1987-03-26
申请号：PCT/US1986001851
申请日：1986-09-09
申请人： COULTER ELECTRONICS, INC.
发明人： COULTER ELECTRONICS, INC. , GRAHAM, Marshall, Donnie
IPC分类号： B01D35/06
CPC分类号： B03C1/032
摘要： An apparatus for dislodging fragile particles, such as intact biological cells, retained by the separation matrix (30) in a flow chamber (12) of a magnetic separation system. The apparatus incorporates a piezoelectric transducer (52) which is coupled to the matrix (30) and an associated drive circuit. The system can operate in a capture phase, whereby fragile particles are selectively captured from a carrier fluid passing through the matrix (30), with those captured particles being magnetically held in place within the matrix (30). In the elutriation phase, an elutriation fluid is passed through the matrix (30) and the drive circuit excites the piezoelectric transducer (52). In response to the excitation, the transducer (52) establishes acoustic waves in the elutriation fluid passing through the matrix (30), vibrating the matrix (30) itself. The acoustic waves can be ultrasonic. The acoustic waves and matrix vibration operate to dislodge the intact cells from the matrix (30), even at relatively low elutriation flow rates.

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