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1. 发明申请

WO2017100792A1 SYSTEMS, METHODS AND COMPOSITIONS FOR ENHANCING THE SPECIFICITY OF NUCLEIC ACID HYBRIDIZATION 审中-公开
标题翻译：用于增强核酸杂交特异性的系统，方法和组合物
公开(公告)号：WO2017100792A1
公开(公告)日：2017-06-15
申请号：PCT/US2016/066227
申请日：2016-12-12
申请人： OKURA, Michael D.
发明人： OKURA, Michael D. , OKURA, Michael
IPC分类号： G01N33/48 , G01N33/50 , C12Q1/68 , C40B30/04 , C40B60/12
CPC分类号： C12Q1/6837 , C12Q1/6832 , C12Q2523/31
摘要： Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.
摘要翻译：根据本发明的物质的系统，方法和组合物可用于捕获/富集，基因表达谱分析和靶向测序。提供了涉及提高核酸杂交特异性和控制由核酸杂种对揭示的解链曲线形状以优化核酸分析的系统，方法和组合物。这些系统，方法和组合物包括在类似目的的微阵列载玻片或其他类型的表面上产生带正电荷的表面或表面涂层，其将熔解曲线分析增强到允许检测或区分核酸之间序列的小变化酸结合伴侣。熔解曲线分析的准确度或分辨率足以区分完全匹配的dsDNA和dsDNA的熔解，其中序列中可能的变化最小，即一个碱基对错配。

2. 发明申请

WO2012109157A2 BIOPROBES AND METHODS OF USE THEREOF 审中-公开
标题翻译：生物学及其使用方法
公开(公告)号：WO2012109157A2
公开(公告)日：2012-08-16
申请号：PCT/US2012/024015
申请日：2012-02-06
申请人： THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO , KELLEY, Shana, O. , FANG, Zhichao , VASILYEVA, Elizaveta
发明人： KELLEY, Shana, O. , FANG, Zhichao , VASILYEVA, Elizaveta
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12N2310/3181 , C12Q1/6837 , C12Q1/6886 , G01N27/26 , G01N27/3276 , G01N27/3277 , C12Q2523/31
摘要： Disclosed are biomolecule based bioprobes that exhibit improved water solubility and mono layer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bio-probe. Further disclosed are suitable electrochemical reporter systems for use with the bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.
摘要翻译：公开了基于生物分子的生物探针，其表现出改善的水溶性和单层形成性质，其基本上很少或没有聚集，其可以明显地干扰生物探针与靶核苷酸的结合。生物探针可以与合适的报道系统结合使用以检测非常少量的生物标志物。生物探针包含能与靶核苷酸杂交的核碱基序列; 和至少一个连接到所述核碱基序列的带电官能团。还公开了生物传感器和包括生物探针的感测装置。进一步公开的是用于生物探针的合适的电化学报告体系。还包括使用这些装置和探针的方法，包括用于检测靶标生物标志物，包括癌细胞或病原体的生物标志物。

3. 发明申请

WO2011031025A3 METHOD FOR DETECTING AND QUANTIFYING A TARGET SUBSTANCE USING A BIOCHIP 审中-公开
标题翻译：用生物薄片检测和定量目标物质的方法
公开(公告)号：WO2011031025A3
公开(公告)日：2011-07-07
申请号：PCT/KR2010005785
申请日：2010-08-27
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , KIM SANG KYU , CHO HYUN MIN
发明人： CHUNG BONG HYUN , KIM SANG KYU , CHO HYUN MIN
IPC分类号： G01N33/68 , G01N33/50
CPC分类号： C12Q1/6837 , C12Q2565/113 , C12Q2563/155 , C12Q2563/137 , C12Q2523/31
摘要： The present invention relates to a method for detecting and quantifying a target substance using a biochip having a substrate onto which probe molecules are fixed, and more particularly, to a method which involves preparing a biochip having a substrate onto which probe molecules are fixed, and enabling the biochip to contact a specimen containing a target substance having electric charges, conducting a reaction between the target substance and nanoparticles having electric charges that are opposite to those of the target substance, and conducting a reaction with a metal enhancing solution such that the sizes of the nanoparticles are amplified and the target substance can be detected and quantified using the biochip which can be seen by the naked eye.
摘要翻译：本发明涉及使用具有固定有探针分子的基体的生物芯片来检测和定量目标物质的方法，更具体地涉及制备具有固定探针分子的基体的生物芯片的方法，以及使生物芯片与包含具有电荷的目标物质的样本接触，使目标物质与具有与目标物质的电荷相反的电荷的纳米粒子之间发生反应，并且与金属增强溶液进行反应，使得尺寸的纳米颗粒被扩增，并且可以使用肉眼可见的生物芯片来检测和定量目标物质。

4. 发明申请

WO2019078722A1 COACERVATE-BASED DETECTION OF A COMPOUND OF INTEREST 审中-公开
公开(公告)号：WO2019078722A1
公开(公告)日：2019-04-25
申请号：PCT/NL2018/050690
申请日：2018-10-19
申请人： TECHNISCHE UNIVERSITEIT DELFT
发明人： REESE, Louis , SPOELSTRA, Willem Kasper , BROUNS, Stan Johan Jozef
IPC分类号： C12Q1/6816
CPC分类号： C12Q1/6816 , C12Q2521/319 , C12Q2523/31 , C12Q2563/113 , C12Q2563/119 , C12Q2565/113
摘要： The present invention relates to the field of biotechnology, more specifically to the field of molecular diagnostics; to a method for coacervate-based detection of the presence of a compound of interest in a sample, wherein upon recognition of the compound of interest by the target recognition moiety, coacervate phase separation is either resolved or induced, wherein the extent of phase separation is a measure for the presence of the compound of interest.

5. 发明申请

WO2017100027A1 METHOD OF TRANSLOCATING NUCLEIC ACIDS THROUGH NANOPORES 审中-公开
公开(公告)号：WO2017100027A1
公开(公告)日：2017-06-15
申请号：PCT/US2016/063877
申请日：2016-11-28
申请人： QUANTAPORE, INC.
发明人： HONG, Tao , GUEGLER, Karl , SIMONS, Jan F.
IPC分类号： C12M1/34 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q2523/31 , C12Q2525/161 , C12Q2565/631
摘要： The invention provides methods for analyzing polynucleotides using nanopores that allow passage of single stranded polynucleotides but not double stranded polynucleotides. In accordance with some embodiments, a double-stranded product is produced that comprises a labeled strand with a single stranded tail or overhang. The double stranded product is exposed to one or more nanopores in the presence of an electric field across the one or more nanopores such that the single stranded tail may be captured and the labeled strand translocated by unzipping from the double stranded product. The ionic composition of the reaction mixture and electric field strength are selected so that nucleotides translocate a nanopore at a rate of less than 1000 nucleotides per second.

6. 发明申请

WO2013016419A1 DEVICES AND METHODS FOR IMMOBILIZING NUCLEIC ACIDS 审中-公开
标题翻译：用于固定核酸的装置和方法
公开(公告)号：WO2013016419A1
公开(公告)日：2013-01-31
申请号：PCT/US2012/048136
申请日：2012-07-25
申请人： OPGEN, INC. , JIANG, Wenlong
发明人： JIANG, Wenlong
IPC分类号： G01N33/48 , G01N21/00
CPC分类号： C12Q1/6806 , B01L3/502761 , B01L2200/0663 , B01L2400/0427 , C12Q1/6825 , C12Q1/689 , C12Q2523/31 , G01N27/447
摘要： Devices and methods for immobilizing nucleic acids on a substrate include a voltage source, and a substrate coupled to the voltage source, in which hydrophobicity of the substrate changes in response to an applied electric field, and a surface of the substrate is coated with a substance that retains nucleic acids.
摘要翻译：用于将核酸固定在基底上的装置和方法包括电压源和耦合到电压源的衬底，其中衬底的疏水性响应于施加的电场而改变，并且衬底的表面涂覆有物质保留核酸。

7. 发明申请

WO2017210748A1 NUCLEIC ACID COLLECTION DEVICE AND METHOD 审中-公开
标题翻译：核酸收集装置和方法
公开(公告)号：WO2017210748A1
公开(公告)日：2017-12-14
申请号：PCT/AU2017/050577
申请日：2017-06-09
申请人： FLINDERS UNIVERSITY OF SOUTH AUSTRALIA
发明人： WALKER, Alexander, William , TEMPLETON, Jennifer , LINACRE, Adrian, Matthew, Thornton
IPC分类号： C12M1/30 , C12Q1/68 , A61F13/38 , B01L3/00
CPC分类号： B01L3/5029 , C12M1/30 , C12Q1/6806 , C12Q2523/31
摘要： The present invention relates to devices for the collection of nucleic acid samples and methods for their use. Certain embodiments of the present disclosure provide a device for collecting a nucleic acid sample from an environment, the device comprising a collection head comprising a positively charged collection surface, wherein the collection surface comprises a saturation volume of between 1 ul and 100 ul, and a handle connected to the collection head.
摘要翻译：本发明涉及用于收集核酸样品的装置及其使用方法。本公开的某些实施方案提供了用于从环境收集核酸样品的装置，所述装置包括具有带正电的收集表面的收集头，其中收集表面包含1μl至100μl的饱和体积和把手连接到收集头。

8. 发明申请

WO2017205477A1 POLYNUCLEOTIDE PURIFICATION WITH MONOLITH COLUMNS 审中-公开
标题翻译：用单体柱纯化多核苷酸
公开(公告)号：WO2017205477A1
公开(公告)日：2017-11-30
申请号：PCT/US2017/034193
申请日：2017-05-24
申请人： ALEXION PHARMACEUTICALS, INC. , DAVIS, Jared , DEBEAR, Joanna , CHENG, Christopher
发明人： DAVIS, Jared , DEBEAR, Joanna , CHENG, Christopher
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2523/31 , C12Q2565/137
摘要： Described herein are methods of purifying polynucleotides, e.g ., imRNA and oligonucleotides, e.g ., probes, primers and siRNA, using monolithic columns with immobilized ligands coupled to the monolithic column. Also described are monolithic columns for purifying polynucleotides from a sample; and methods of preparing such columns.
摘要翻译：本文描述了使用整体柱纯化多核苷酸，例如，imRNA和寡核苷酸，例如探针，引物和siRNA的方法，其中使用整体柱与整体柱偶联的固定配体。还描述了纯化来自样品的多核苷酸的整体柱; 以及制备这种色谱柱的方法。

9. 发明申请

WO2011100669A3 LABLE-FREE MULTIPLEXING BIOASSAYS USING FLUORESCENT CONJUGATED POLYMERS AND BARCODED NANOPARTICLES 审中-公开
标题翻译：使用荧光共聚聚合物和BARCODED NANOPARTICLES的无线多路复用生物多样性
公开(公告)号：WO2011100669A3
公开(公告)日：2012-01-12
申请号：PCT/US2011024730
申请日：2011-02-14
申请人： UNIV NORTH CAROLINA STATE , HE LIN , ZHENG WEIMING
发明人： HE LIN , ZHENG WEIMING
IPC分类号： C12Q1/68 , G01N33/52 , G01N33/58
CPC分类号： G01N33/54313 , C12Q1/6816 , C12Q2523/31 , C12Q2523/313 , C12Q2563/137 , C12Q2563/155 , C12Q2565/514
摘要： Label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods are disclosed. Different DNA capture probes are encoded by the different reflectivity of Au and Ag stripe patterns. The integration of fluorescent conjugated polymers as detection moieties with metallic striped nanorods for multiplexed detection of clinically important cancer marker proteins in an immunoassay format is also provided.
摘要翻译：公开了使用荧光共轭聚合物作为检测探针的无标记多重DNA测定来说明金属条纹纳米棒上的杂交。不同的DNA捕获探针由Au和Ag条纹图案的不同反射率编码。还提供了荧光共轭聚合物作为检测部分与金属条状纳米棒的整合，用于以免疫测定方式多重检测临床上重要的癌症标志物蛋白质。

10. 发明申请

WO2011031025A2 바이오칩을 이용한 표적 물질 검출 및 정량 방법 审中-公开
标题翻译：使用BIOCHIP检测和定量目标物质的方法
公开(公告)号：WO2011031025A2
公开(公告)日：2011-03-17
申请号：PCT/KR2010/005785
申请日：2010-08-27
申请人： 한국생명공학연구원 , 정봉현 , 김상규 , 조현민
发明人： 정봉현 , 김상규 , 조현민
IPC分类号： G01N33/68 , G01N33/50
CPC分类号： C12Q1/6837 , C12Q2565/113 , C12Q2563/155 , C12Q2563/137 , C12Q2523/31
摘要： 본 발명은 기판 상에 프로브 분자가 고정된 바이오칩을 이용하여, 표적 물질을 검출하는 방법 및 정량 방법에 관한 것으로서, 보다 상세하게는 기판 상에 프로브 분자가 고정된 바이오칩을 준비하고, 이를 전하를 갖는 표적 물질을 포함하는 시료와 접촉시킨 후, 상기 표적 물질과 반대 전하를 갖는 나노 입자를 반응시킨 후 금속 인핸싱 용액 (enhancing solution)을 반응시켜서 상기 나노 입자의 크기를 증폭하여 육안으로 바이오칩을 이용한 표적 물질의 검출 방법 및 정량 방법에 관한 것이다.
摘要翻译：本发明涉及一种使用具有固定有探针分子的基板的生物芯片来检测和定量目标物质的方法，更具体地涉及制备具有固定有探针分子的基板的生物芯片的方法，以及使生物芯片与包含具有电荷的目标物质的试样接触，进行目标物质与具有与靶物质相反的电荷的纳米粒子的反应，与金属增强溶液进行反应，的纳米颗粒被扩增，并且可以使用肉眼可以看到的生物芯片来检测和定量目标物质。

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