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1. 发明申请

WO2019051253A1 MODIFIED DNA POLYMERASES 审中-公开
公开(公告)号：WO2019051253A1
公开(公告)日：2019-03-14
申请号：PCT/US2018/049993
申请日：2018-09-07
申请人： SIGMA-ALDRICH CO. LLC , MERCK PATENT GMBH
发明人： BAIGA, Thomas , BURLEY, Michael Anderson , SMITH, Alexander
IPC分类号： C12N15/54 , C12N9/12
CPC分类号： C12N9/1252 , C07H21/00 , C12N15/1068 , C12N15/1096 , C12N15/52 , C12P19/34 , C12Q1/6806 , C12Q1/6844 , C12Q2521/101 , C12Q2525/117 , C12Y207/07001
摘要： Modified X family DNA polymerases engineered to be capable of incorporating 3'-O-blocked nucleotide 5'-triphosphates during template-independent polynucleotide synthesis, and methods for synthesizing polynucleotides using said modified X family DNA polymerases.

2. 发明申请

WO2018229173A1 COMPOSITIONS AND METHODS FOR IMPROVING THE THERMAL STABILITY OF NUCLEIC ACID AMPLIFICATION REAGENTS 审中-公开
公开(公告)号：WO2018229173A1
公开(公告)日：2018-12-20
申请号：PCT/EP2018/065753
申请日：2018-06-14
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ROCHE MOLECULAR SYSTEMS, INC.
发明人： GUPTA, Amar
IPC分类号： C12Q1/686
CPC分类号： C12Q1/6853 , C12N15/1096 , C12N2310/344 , C12Q1/686 , C12Q2521/101 , C12Q2525/117 , C12Q2563/107
摘要： The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises nucleoside polyphosphates having four or more phosphates. The present invention also provides for methods for using the nucleoside polyphosphates having four or more phosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

3. 发明申请

WO2017048982A1 OLIGOPHOSPHATE DARK QUENCHERS AND USES 审中-公开
标题翻译：低磷酸盐低密度猝灭剂和用途
公开(公告)号：WO2017048982A1
公开(公告)日：2017-03-23
申请号：PCT/US2016/051976
申请日：2016-09-15
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： DEMPCY, Robert , WONG, Albert , JOHANSSON, Hans , COOK, Ronald, M.
IPC分类号： C12Q1/68 , C07F9/24
CPC分类号： C07F9/65583 , C07F9/6561 , C12Q1/68 , C12Q1/6818 , C12Q2525/117 , C12Q2565/1015
摘要： The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.
摘要翻译：本发明提供了包含这些猝灭剂的激发态能量猝灭剂，探针和其它共轭物及其使用方法。

4. 发明申请

WO2017040477A1 COMPOSITION AND METHODS FOR DETECTING ADENOSINE MODIFICATIONS 审中-公开
标题翻译：用于检测腺苷修饰的组合物和方法
公开(公告)号：WO2017040477A1
公开(公告)日：2017-03-09
申请号：PCT/US2016/049407
申请日：2016-08-30
申请人： THE UNIVERSITY OF CHICAGO
发明人： HE, Chuan , CHEN, Kai , DAI, Qing
IPC分类号： A61K38/48 , C12N9/78
CPC分类号： C12N9/78 , C12Q1/34 , C12Q1/6806 , C12Q1/6827 , C12Q1/6869 , G01N2333/978 , C12Q2521/539 , C12Q2525/117
摘要： The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence is adenosine.
摘要翻译：本公开涉及用于检测靶RNA分子中修饰的腺苷的方法，组合物和试剂盒。方面涉及用于检测目标核糖核酸（RNA）中修饰的腺苷的方法，包括使靶RNA与腺苷脱氨酶（腺苷脱氨酶，RNA特异性）接触以产生具有脱氨基腺苷的靶RNA，并用脱氨酸测序靶RNA 腺苷; 其中当所述核苷酸序列是腺苷时检测到修饰的腺苷。

5. 发明申请

WO2016172701A2 OLIGONUCLEOTIDE-BASED PROBES FOR DETECTION OF CIRCULATING TUMOR CELL NUCLEASES 审中-公开
标题翻译：用于检测循环肿瘤细胞核的基于寡核苷酸的探针
公开(公告)号：WO2016172701A2
公开(公告)日：2016-10-27
申请号：PCT/US2016/029201
申请日：2016-04-25
申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION , GIANGRANDE, Paloma, H. , MCNAMARA, James, O. , DICKEY, David, D. , OZER, Howard , KRUSPE, Sven
发明人： GIANGRANDE, Paloma, H. , MCNAMARA, James, O. , DICKEY, David, D. , OZER, Howard , KRUSPE, Sven
IPC分类号： C12Q1/68 , C09B56/00
CPC分类号： C12Q1/68 , C12Q1/6816 , C12Q1/6886 , C12Q2525/117 , C12Q2525/125
摘要： The present invention relates to a rapid detection of circulating tumor cell (CTC)-associated nuclease activity with chemically modified nuclease substrate probes and compositions useful in detection assays.
摘要翻译：本发明涉及利用化学修饰的核酸酶底物探针和用于检测测定的组合物快速检测循环肿瘤细胞（CTC）相关核酸酶活性。

6. 发明申请

WO2016153999A1 MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开
标题翻译：改良的核苷酸及其用途
公开(公告)号：WO2016153999A1
公开(公告)日：2016-09-29
申请号：PCT/US2016/023139
申请日：2016-03-18
申请人： LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES GMBH
发明人： HINZ, Wolfgang , MENCHEN, Steven , GRAHAM, Ronald , VANDER HORN, Peter , HUBBELL, Earl , WOEHLER, Christian
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q2525/117 , C12Q2565/30
摘要： In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.
摘要翻译：在一些实施方案中，本公开一般涉及涉及在模板依赖性中使用经修饰的核苷酸（包括终止子核苷酸和/或标记的核苷酸）的核酸分析的方法以及相关的系统，组合物，试剂盒和装置核苷酸掺入反应。在一些实施方案中，核酸分析可以在单个反应位点或反应位点阵列中的多个反应位点进行。任选地，阵列包含多个具有约1-100百万，约100-250百万，或约200-500百万，或约500-900万个或更多个反应位点的反应位点。任选地，每个反应位点与作为离子敏感FET（isFET）或化学敏感FET（chemFET）传感器的一个或多个传感器接触，可操作地耦合或电容耦合。任选地，反应位点彼此流体连通。

7. 发明申请

WO2016083354A1 DETECTING SINGLE NUCLEOTIDE POLYMORPHISM USING OVERLAPPING HYDROLYSIS PROBES 审中-公开
标题翻译：使用重叠水解探针检测单核多核苷酸多态性
公开(公告)号：WO2016083354A1
公开(公告)日：2016-06-02
申请号：PCT/EP2015/077466
申请日：2015-11-24
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ROCHE MOLECULAR SYSTEMS, INC.
发明人： MEHTA, Rochak
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6818 , C12Q1/6827 , C12Q1/6848 , C12Q1/6851 , C12Q1/686 , C12Q2525/117 , C12Q2525/301 , C12Q2535/131 , C12Q2537/1373 , C12Q2537/161 , C12Q2561/101
摘要： Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping SNP specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3' end, and a detecting step. Furthermore, the double stranded SNP specific hydrolysis probes along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.
摘要翻译：描述了用于快速检测样品中目标核酸中SNP存在或不存在的方法。所述方法可以包括进行放大步骤，利用具有两个重叠SNP特异性水解探针序列的双链探针的杂交步骤，其中探针序列之一可以包括朝向3'端的发夹结构，以及检测步骤。此外，提供双链SNP特异性水解探针以及试剂盒，其被设计用于检测靶核酸中的SNP。

8. 发明申请

WO2016001314A1 RATIONALLY DESIGNED 2'-(H-1,2,3-TRIAZOYL)-METHOXY OLIGONUCLEOTIDE PROBES FOR FLUORESENCE-BASED BIOANALYSIS 审中-公开
标题翻译：用于基于荧光的生物分析的方法设计的2' - （H-1,2,3-三唑基） - 甲基寡核苷酸探针
公开(公告)号：WO2016001314A1
公开(公告)日：2016-01-07
申请号：PCT/EP2015/064999
申请日：2015-07-01
申请人： SYDDANSK UNIVERSITET
发明人： ASTAKHOVA, Irina Kira
IPC分类号： C07H21/00 , C07H21/02 , C12Q1/68
CPC分类号： C07H21/00 , C07H21/02 , C12Q1/6816 , C12Q2525/117 , C12Q2563/107
摘要： The invention provides rationally designed 2'-(H-1,2,3-triazoyl)-methoxy oligonucleotides for fluorescence-based bioanalysis. They can be readily synthesised via "click chemistry" on the corresponding oligonucleotides comprising 2'-O-alkylnyl nucleotides.
摘要翻译：本发明提供合理设计的用于基于荧光的生物分析的2' - （H-1,2,3-三唑酰） - 甲氧基寡核苷酸。它们可以通过“点击化学”在包含2'-O-烷基核苷酸的相应寡核苷酸上容易地合成。

9. 发明申请

WO2015153510A1 MODIFIED CYTOSINE POLYNUCLEOTIDE OLIGOMERS AND METHODS 审中-公开
标题翻译：改性的胞质多核苷酸寡聚体和方法
公开(公告)号：WO2015153510A1
公开(公告)日：2015-10-08
申请号：PCT/US2015/023428
申请日：2015-03-30
申请人： CEPHEID
发明人： GALL, Alexander A. , LOKHOV, Sergey G. , PODYMINOGIN, Mikhail A. , VIAZOVKINA, Ekaterina V. , LUND, Kevin Patrick
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C07H19/10 , C07H19/06 , C07K5/06078 , C12N15/11 , C12N15/111 , C12N2310/311 , C12N2310/312 , C12N2310/3125 , C12N2310/314 , C12N2310/315 , C12N2310/3181 , C12N2310/32 , C12N2310/3231 , C12N2310/334 , C12N2310/3513 , C12N2320/52 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2525/117 , C12Q2525/107 , C12Q2525/113
摘要： Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
摘要翻译：公开了修饰的胞嘧啶碱基，其在多核苷酸杂交复合物中提供对鸟嘌呤碱基的增强的碱基配对亲和力。还公开了包含这种修饰的胞嘧啶碱基的多核苷酸寡聚体，多核苷酸杂交复合物。还公开了各种使用方法。例如，在一些实施方案中，本文公开的修饰的多核苷酸寡聚体可用作核酸扩增和/或检测的引物和探针。

10. 发明申请

WO2015153496A1 MODIFIED THYMINE POLYNUCLEOTIDE OLIGOMERS AND METHODS 审中-公开
标题翻译：改性的多胺多聚体低聚物和方法
公开(公告)号：WO2015153496A1
公开(公告)日：2015-10-08
申请号：PCT/US2015/023403
申请日：2015-03-30
申请人： CEPHEID
发明人： GALL, Alexander A. , LOKHOV, Sergey G. , PODYMINOGIN, Mikhail , VIAZOVKINA, Ekaterina V. , LUND, Kevin Patrick
IPC分类号： C07H19/06 , C07H21/00 , C12Q1/68
CPC分类号： C07H19/06 , C07F9/65744 , C07H19/073 , C07H19/10 , C07H21/00 , C07H21/04 , C07K14/003 , C12Q1/6813 , C12Q1/6832 , C12Q2525/117
摘要： Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
摘要翻译：公开了修饰的胸腺嘧啶碱基，其在多核苷酸杂交复合物中对腺嘌呤或2,6-二氨基嘌呤碱基提供增强的碱基配对亲和力。还公开了包含这种修饰的胸腺嘧啶碱基的多核苷酸寡聚体，多核苷酸杂交复合物。还公开了各种使用方法。例如，在一些实施方案中，本文公开的修饰的多核苷酸寡聚体可用作核酸扩增和/或检测的引物和探针。

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