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1. 发明申请

WO99001688A1 THERMAL MICROVALVES 审中-公开
标题翻译：热微波
公开(公告)号：WO99001688A1
公开(公告)日：1999-01-14
申请号：PCT/US1998/013819
申请日：1998-07-02
IPC分类号： B81B3/00 , B01F5/04 , B01F13/00 , B01J19/00 , B01L3/00 , B01L7/00 , B81C1/00 , C12M1/34 , C12Q1/68 , F15B13/00 , F15B13/08 , F15C5/00 , F16K31/02 , F16K31/64 , F16K99/00 , G01N37/00 , H01L27/14
CPC分类号： B01F13/0071 , B01F5/0085 , B01F5/0403 , B01F13/0072 , B01F13/0084 , B01F13/0093 , B01F15/0232 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00828 , B01J2219/00831 , B01J2219/00837 , B01J2219/00853 , B01J2219/0086 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/00957 , B01J2219/00961 , B01J2219/0097 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L7/52 , B01L2200/0605 , B01L2300/0816 , B01L2300/1827 , B01L2400/0442 , B01L2400/0487 , B01L2400/0677 , B01L2400/0688 , B01L2400/0694 , B82Y30/00 , F15B13/0807 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0017 , F16K99/0019 , F16K99/0034 , F16K99/0044 , F16K99/0061 , F16K2099/0071 , F16K2099/0074 , F16K2099/0076 , F16K2099/008 , F16K2099/0084 , Y10S366/04 , Y10T137/1812 , Y10T436/2575
摘要： Thermal microvalves and their method of use include a fusible material (40) positioned in a microchannel (44). A heater (42) melts the material (40) which is then forced by air pressure (46) into a second channel (48) where it cools and solidifies, blocking flow. Alternatively, the melted and solidified material (40) can hold a diaphragm of a diaphragm valve in place, eliminating the need for continued applied force on the diaphragm.
摘要翻译：热微型阀及其使用方法包括位于微通道（44）中的可熔材料（40）。加热器（42）熔化材料（40），然后将材料（40）通过空气压力（46）压入第二通道（48），在第二通道（48）中冷却和固化，阻止流动。或者，熔融和固化的材料（40）可以将隔膜阀的隔膜保持就位，从而不需要在隔膜上继续施加力。

2. 发明申请

WO2008032276A2 METHODS AND DEVICES FOR SAMPLING FLOWABLE MATERIALS 审中-公开
标题翻译：用于采样可流动材料的方法和设备
公开(公告)号：WO2008032276A2
公开(公告)日：2008-03-20
申请号：PCT/IB2007/053678
申请日：2007-09-12
申请人： INSTITUT CURIE , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , VIOVY, Jean-Louis , CHABERT, Max
发明人： VIOVY, Jean-Louis , CHABERT, Max
IPC分类号： B01L3/00 , G01N35/10
CPC分类号： G01N35/1009 , B01F13/0072 , B01L3/021 , B01L3/0293 , B01L3/502715 , B01L3/502769 , B01L3/502784 , B01L7/525 , B01L2200/027 , B01L2200/0673 , B01L2300/0867 , B01L2400/0406 , B01L2400/0487 , G01N35/08 , G01N2035/00277 , Y10T137/0318 , Y10T137/85938 , Y10T436/25 , Y10T436/2575
摘要： The present invention concern a method for sampling and mixing different flowable material materials from a plurality of wells in arbitrary order.
摘要翻译：本发明涉及一种以任意顺序从多个井取样和混合不同可流动材料的方法。

3. 发明申请

WO0194635A9 INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS 审中-公开
标题翻译：集成有源通量微流体器件和方法
公开(公告)号：WO0194635A9
公开(公告)日：2002-08-08
申请号：PCT/US0118400
申请日：2001-06-05
申请人： CALIFORNIA INST OF TECHN , QUAKE STEPHEN R , CHOU HOU-PU
发明人： QUAKE STEPHEN R , CHOU HOU-PU
IPC分类号： G01N21/64 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L99/00 , B81B1/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N21/05 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , B01J19/00
CPC分类号： B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
摘要翻译：本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其他分子的微制造装置。本发明的装置和方法可以用于通过检测分子（例如分子数量），例如多核苷酸（例如DNA）或蛋白质（例如抗体），通过测量可检测信号来同时诊断多种疾病报道子与杂交的多核苷酸或抗原/抗体复合物相关。在根据本发明的微制造装置中，检测分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在与来自与结合分子相关的光学可检测（例如荧光）报道分子的杂交信号相关。这些杂交信号可以通过任何合适的手段检测，例如光学检测，并且可以例如作为特定基因存在的表示存储在计算机中。可以将杂交探针固定在形成一部分或暴露于形成闭合环路的装置的一个或多个通道的基底上，以使样品循环以主动接触互补探针。根据本发明的通用芯片不仅可以用DNA制造，而且可以用其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子制造。

4. 发明申请

WO2008032276A3 METHODS AND DEVICES FOR SAMPLING FLOWABLE MATERIALS 审中-公开
标题翻译：用于采样流动材料的方法和装置
公开(公告)号：WO2008032276A3
公开(公告)日：2008-08-21
申请号：PCT/IB2007053678
申请日：2007-09-12
申请人： INST CURIE , CENTRE NAT RECH SCIENT , VIOVY JEAN-LOUIS , CHABERT MAX
发明人： VIOVY JEAN-LOUIS , CHABERT MAX
IPC分类号： B01L3/00 , G01N35/10
CPC分类号： G01N35/1009 , B01F13/0072 , B01L3/021 , B01L3/0293 , B01L3/502715 , B01L3/502769 , B01L3/502784 , B01L7/525 , B01L2200/027 , B01L2200/0673 , B01L2300/0867 , B01L2400/0406 , B01L2400/0487 , G01N35/08 , G01N2035/00277 , Y10T137/0318 , Y10T137/85938 , Y10T436/25 , Y10T436/2575
摘要： The present invention concern a method for sampling and mixing different flowable material materials from a plurality of wells in arbitrary order.
摘要翻译：本发明涉及从多个孔以任意顺序取样和混合不同的可流动材料材料的方法。

5. 发明申请

WO2005111629A1 MICROANALYSIS APPARATUS WITH CONSTANT PRESSURE PUMP SYSTEM 审中-公开
标题翻译：具有恒压泵系统的微观分析装置
公开(公告)号：WO2005111629A1
公开(公告)日：2005-11-24
申请号：PCT/DK2005/000321
申请日：2005-05-13
申请人： DANFOSS A/S , GRAVESEN, Peter
发明人： GRAVESEN, Peter
IPC分类号： G01N35/08
CPC分类号： G01N35/085 , A61B5/14532 , B01F13/0072 , B01F13/0093
摘要： Micro-analysis system for analysing species with a fluid medium, said system containing sensing means for collecting species from the medium, said sensing means having an inlet and an outlet, analysing means comprising a channel, said channel defining at least one part for mixing, at least one part for reacting and at least one part for measuring detecting arrangement for determining the concentration of species at said part for measuring, a first fluid reservoir holding carrier fluid and at least one second fluid reservoir holding reagent fluid, connecting means, comprising first connecting means for fluid connection between the first fluid reservoir and the analysing means, second connecting means for fluid connection between the second fluid reservoir and the inlet of the sensing means and third connecting means for fluid connection between the outlet of the sensing means and the analysing means, said first connecting means comprising at least one first flow restricting means and said second connecting means comprising at least one second flow restricting means characterised by said micro-analysis system further comprising storage means containing said first fluid reservoir and said second fluid reservoir, said storage means being in downstream flow connection with means for pressurizing said fluid reservoirs, said storage means and said pressurizing means being separated from said analysing means.
摘要翻译：用于用流体介质分析物种的微分析系统，所述系统包含用于从介质收集物质的感测装置，所述感测装置具有入口和出口，包括通道的分析装置，所述通道限定至少一个用于混合的部分，用于反应的至少一个部分和用于测量用于测量所述部分处的物质浓度的检测装置的至少一个部分，第一流体储存器保持载体流体和至少一个第二流体储存器保持试剂流体，连接装置，包括第一用于在第一流体储存器和分析装置之间流体连接的连接装置，用于在第二流体储存器和感测装置的入口之间流体连接的第二连接装置和用于感测装置的出口和分析装置之间的流体连接的第三连接装置装置，所述第一连接装置包括至少一个第一流动限制装置和sa id第二连接装置，包括至少一个第二流动限制装置，其特征在于所述微分析系统还包括容纳所述第一流体储存器和所述第二流体储存器的存储装置，所述存储装置与用于对所述流体储存器加压的装置处于下游流动连接，所述存储装置和所述加压装置与所述分析装置分离。

6. 发明申请

WO0194635A3 INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS 审中-公开
标题翻译：集成的主动流量微流体装置和方法
公开(公告)号：WO0194635A3
公开(公告)日：2002-10-31
申请号：PCT/US0118400
申请日：2001-06-05
申请人： CALIFORNIA INST OF TECHN , QUAKE STEPHEN R , CHOU HOU-PU
发明人： QUAKE STEPHEN R , CHOU HOU-PU
IPC分类号： G01N21/64 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L99/00 , B81B1/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N21/05 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/58 , G01N37/00 , B01F5/10 , B01J19/00
CPC分类号： B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
摘要翻译：本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病。与杂交多核苷酸或抗原/抗体复合物相关的报道。在根据本发明的微制造装置中，分子（即多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。杂交探针可以固定在形成一部分或暴露于形成闭环的装置的通道或通道的基底上，用于循环样品以主动接触互补探针。根据本发明的通用芯片不仅可以用DNA而且可以与其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子一起制备。

7. 发明申请

WO2014141861A1 マイクロ流路装置及びこれに関する方法审中-公开
标题翻译： MICROCHANNEL设备和方法
公开(公告)号：WO2014141861A1
公开(公告)日：2014-09-18
申请号：PCT/JP2014/054299
申请日：2014-02-24
申请人：国立大学法人筑波大学
发明人：福田　淳二 , 鈴木　博章 , 山岸　安奈 , 榎本　詢子 , 横川　雅俊
IPC分类号： C12Q1/02 , C12M1/00 , C12M3/00 , C12Q3/00
CPC分类号： B01L3/502784 , B01F3/0803 , B01F5/0085 , B01F13/0061 , B01F13/0069 , B01F13/0071 , B01F13/0072 , B01L3/0293 , B01L3/5027 , B01L3/502792 , B01L2200/0605 , B01L2300/088 , C12M23/16 , G01N1/38
摘要：　微量液体の効果的な操作を実現するマイクロ流路装置及びこれに関する方法を提供する。本発明に係るマイクロ流路装置（１）は、上流液体プラグ及び気体を流すための上流流路部（１０）と、下流液体プラグ及び気体を流すための下流流路部（２０）と、前記上流流路部の下流端部と前記下流流路部の上流端部との間に設けられ主液体プラグを保持するための液体保持部（３０）と、前記上流流路部の前記下流端部から前記下流流路部の前記上流端部まで前記液体保持部を迂回するように設けられ、前記液体保持部が前記主液体プラグを保持した状態で気体を流すための気体バイパス流路部（４０）と、を備えている。
摘要翻译：提供实现微量液体的有效操作的微通道装置及其方法。该微通道装置（1）设置有：上游液塞和用于气体通过的上游通道单元（10）; 下游液塞和用于气体通过的下游通道单元（20）; 液体保持单元，用于保持设置在上游通道单元的下游端和下游通道单元的上游端之间的主液塞; 以及气体旁路通道单元（40），其在所述液体保持单元保持所述主液塞的状态下通过气体，所述气体旁通通道单元设置成绕所述液体保持单元从所述上游通道单元的下游端到所述上游下游通道单元的末端。

8. 发明申请

WO2013192351A1 STABILIZED DROPLETS FOR CALIBRATION AND TESTING 审中-公开
标题翻译：用于校准和测试的稳定液滴
公开(公告)号：WO2013192351A1
公开(公告)日：2013-12-27
申请号：PCT/US2013/046654
申请日：2013-06-19
申请人： BIO-RAD LABORATORIES, INC. , HIDDESSEN, Amy , STEENBLOCK, Erin
发明人： HIDDESSEN, Amy , STEENBLOCK, Erin
IPC分类号： C12Q1/00 , G01N33/48 , C12Q1/68 , C12M1/38
CPC分类号： C12Q1/6816 , B01F5/0085 , B01F5/0647 , B01F9/00 , B01F11/00 , B01F13/0062 , B01F13/0072 , B01F2215/0037 , B01L3/502784 , B01L3/52 , B01L2200/148 , B01L2200/185 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , C12Q1/6806 , G01N1/38 , G01N21/6428 , G01N21/645 , G01N2001/2893 , G01N2021/6439 , G01N2201/127 , C12Q2563/159
摘要： Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.
摘要翻译：本文提供了液滴混合物组合物和用于形成液滴混合物的系统和方法。该系统可以包括两个或更多个液滴产生单元。每个单元可以包括至少一个第一输入井，第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。组合的液滴种群可以混合，加热和收集用于多种用途，例如用作仪器测试和分析的校准标准。

9. 发明申请

WO2011078777A1 METHOD OF ANALYSIS WITH IMPROVED MIXING 审中-公开
标题翻译：改进混合分析方法
公开(公告)号：WO2011078777A1
公开(公告)日：2011-06-30
申请号：PCT/SE2010/051446
申请日：2010-12-21
申请人： GE HEALTHCARE BIO-SCIENCES AB , JANSSON, Östen
发明人： JANSSON, Östen
IPC分类号： G01N35/08 , B01F3/08 , B01F5/04 , G01N21/55 , G01N33/543
CPC分类号： G01N21/553 , B01F13/0072 , G01N21/05 , G01N33/54373 , G01N35/1065 , G01N35/1095
摘要： The invention is a method for characterizing an interaction in a liquid environment, between at least one species in solution and a target immobilized on a surface of a flow cell. The method comprises the following steps: (a) activating the surface of the flow cell and immobilizing the target thereon; (b) providing, in a flow of liquid, at least one of the species; (c) passing the flow of liquid comprising at least one of the species through the surface of the flow cell which contains the immobilized target; and (d) detecting a result of an interaction between the at least one species and the target using surface plasmon resonance (SPR) technique. The improvement of the method comprises in at least one of steps (a) or (b), inline mixing at least two liquid solutions to generate a mixed solution before it is passed through the surface of the flow cell.
摘要翻译：本发明是用于表征液体环境中的溶液中的至少一种与固定在流动池表面上的靶的相互作用的方法。该方法包括以下步骤：（a）活化流动池的表面并将靶固定在其上; （b）在液体流中提供至少一种物种; （c）使包含至少一种物质的液体流通过含有固定化靶的流动池的表面; 和（d）使用表面等离子体共振（SPR）技术检测至少一种物种与目标物之间的相互作用的结果。该方法的改进包括在步骤（a）或（b）中的至少一个中，将至少两种液体溶液串联混合以在混合溶液通过流动池的表面之前产生混合溶液。

10. 发明申请

WO2001094635A2 INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS 审中-公开
标题翻译：集成的主动流量微流体装置和方法
公开(公告)号：WO2001094635A2
公开(公告)日：2001-12-13
申请号：PCT/US2001/018400
申请日：2001-06-05
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , QUAKE, Stephen, R. , CHOU, Hou-Pu
发明人： QUAKE, Stephen, R. , CHOU, Hou-Pu
IPC分类号： C12Q1/68
CPC分类号： B01L3/02 , B01F5/0646 , B01F5/0647 , B01F13/0072 , B01F13/0093 , B01F15/0201 , B01F15/024 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2300/0867 , B01L2300/088 , B01L2300/0887 , B01L2300/123 , B01L2400/0418 , B01L2400/043 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , B01L2400/0694 , C12M1/02 , C12Q1/6816 , G01N33/53 , Y10T137/0318 , Y10T137/0329 , Y10T137/2218 , Y10T137/2224 , Y10T436/11 , Y10T436/145555 , Y10T436/173845 , Y10T436/2575 , C12Q2565/629
摘要： The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
摘要翻译：本发明涉及用于快速检测与特定疾病相关的DNA，蛋白质或其它分子的微制造装置。本发明的装置和方法可用于通过检测分子（例如数量的分子），例如多核苷酸（例如DNA）或蛋白质（例如抗体）来同时诊断多种疾病，通过测量可检测的与杂交多核苷酸或抗原/抗体复合物相关的报道。在根据本发明的微制造装置中，分子（即，多核苷酸，蛋白质或抗原/抗体复合物）的存在的检测与来自与结合分子相关的光学可检测（例如荧光）报告物的杂交信号相关。这些杂交信号可以通过任何合适的方式，例如光学检测，并且可以例如存储在计算机中作为特定基因的存在的表示。杂交探针可以固定在形成一部分或暴露于形成闭环的装置的通道或通道的基底上，用于循环样品以主动接触互补探针。根据本发明的通用芯片不仅可以用DNA而且可以与其他分子如RNA，蛋白质，肽核酸（PNA）和聚酰胺分子一起制备。

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