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1. 发明申请

WO2012061444A3 SYSTEM FOR FORMING EMULSIONS 审中-公开
公开(公告)号：WO2012061444A3
公开(公告)日：2012-05-10
申请号：PCT/US2011/058857
申请日：2011-11-01
申请人： HIDDESSEN, Amy L. , MASQUELIER, Donald A. , NESS, Kevin D. , HINDSON, Benjamin J. , MAKAREWICZ, Anthony J., Jr. , STEENBLOCK, Erin R.
发明人： HIDDESSEN, Amy L. , MASQUELIER, Donald A. , NESS, Kevin D. , HINDSON, Benjamin J. , MAKAREWICZ, Anthony J., Jr. , STEENBLOCK, Erin R.
IPC分类号： B01F15/00
摘要： System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.

2. 发明申请

WO2014145582A3 SILICONE SURFACTANTS FOR EMULSION ASSAYS 审中-公开
公开(公告)号：WO2014145582A3
公开(公告)日：2014-09-18
申请号：PCT/US2014/030374
申请日：2014-03-17
申请人： BIO-RAD LABORATORIES, INC. , CHIA, Erin, R. , HIDDESSEN, Amy, L. , HINDSON, Benjamin, J. , LOWE, Adam , HAN, Chunxiao
发明人： CHIA, Erin, R. , HIDDESSEN, Amy, L. , HINDSON, Benjamin, J. , LOWE, Adam , HAN, Chunxiao
IPC分类号： C08L83/06
摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

3. 发明申请

WO2013192351A1 STABILIZED DROPLETS FOR CALIBRATION AND TESTING 审中-公开
标题翻译：用于校准和测试的稳定液滴
公开(公告)号：WO2013192351A1
公开(公告)日：2013-12-27
申请号：PCT/US2013/046654
申请日：2013-06-19
申请人： BIO-RAD LABORATORIES, INC. , HIDDESSEN, Amy , STEENBLOCK, Erin
发明人： HIDDESSEN, Amy , STEENBLOCK, Erin
IPC分类号： C12Q1/00 , G01N33/48 , C12Q1/68 , C12M1/38
CPC分类号： C12Q1/6816 , B01F5/0085 , B01F5/0647 , B01F9/00 , B01F11/00 , B01F13/0062 , B01F13/0072 , B01F2215/0037 , B01L3/502784 , B01L3/52 , B01L2200/148 , B01L2200/185 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , C12Q1/6806 , G01N1/38 , G01N21/6428 , G01N21/645 , G01N2001/2893 , G01N2021/6439 , G01N2201/127 , C12Q2563/159
摘要： Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.
摘要翻译：本文提供了液滴混合物组合物和用于形成液滴混合物的系统和方法。该系统可以包括两个或更多个液滴产生单元。每个单元可以包括至少一个第一输入井，第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。组合的液滴种群可以混合，加热和收集用于多种用途，例如用作仪器测试和分析的校准标准。

4. 发明申请

WO2014039912A1 COMPOSITIONS, SYSTEMS AND METHODS FOR DROPLET FORMATION, SPACING AND DETECTION 审中-公开
标题翻译：组合物，系统和方法，用于形成，间隔和检测
公开(公告)号：WO2014039912A1
公开(公告)日：2014-03-13
申请号：PCT/US2013/058631
申请日：2013-09-06
申请人： BIO-RAD LABORATORIES, INC. , STEENBLOCK, Erin , HIDDESSEN, Amy, L. , HINDSON, Ben , NESS, Kevin
发明人： STEENBLOCK, Erin , HIDDESSEN, Amy, L. , HINDSON, Ben , NESS, Kevin
IPC分类号： C12Q1/00 , G01N35/08 , G01N21/64
CPC分类号： C12Q1/686 , B01F3/0807 , B01F13/0062 , B01L3/502784 , B01L7/52 , B01L2200/061 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2400/0487 , G01N15/1404 , G01N21/64 , G01N2015/1486 , G01N2021/6441
摘要： The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids.
摘要翻译：本公开提供用于形成，间隔和/或检测液滴的测定和装置。液滴可以是由两种或更多种不混溶流体组成的乳液。乳液可以是双重乳液，例如存在于连续水相中的油包水油滴。当油包水滴与一种或多种含水流体物流接触时，可以形成双重乳液。本公开还提供了可以添加到流体中的各种添加剂。

5. 发明申请

WO2014039587A1 SYSTEMS AND METHODS FOR STABILIZING DROPLETS 审中-公开
标题翻译：用于稳定滴液的系统和方法
公开(公告)号：WO2014039587A1
公开(公告)日：2014-03-13
申请号：PCT/US2013/058103
申请日：2013-09-04
申请人： BIO-RAD LABORATORIES, INC. , HIDDESSEN, Amy, L. , STEENBLOCK, Erin
发明人： HIDDESSEN, Amy, L. , STEENBLOCK, Erin
IPC分类号： G01N33/48 , C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6806 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2400/0487
摘要： This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir.
摘要翻译：本公开提供了用于样品制备和/或分析的系统，装置和方法，其包括具有与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道的液滴发生器。第一通道和第二通道可以在从样品储存器接收样品和来自载体流体储存器的载体流体的交叉点处相遇，并且产生沿着液滴通道流动到液滴储存器的一个或多个液滴。与交叉点热连通的能量施加构件，液滴通道，样品储存器和/或载体流体储存器的至少一部分可以向一个或多个液滴的单个液滴（例如在交叉点处）提供能量，并且 /或当液滴沿着液滴通道移动到液滴储存器时。

6. 发明申请

WO2012061442A1 ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS 审中-公开
标题翻译：分散在DROPLETS中的残留的基因组DNA
公开(公告)号：WO2012061442A1
公开(公告)日：2012-05-10
申请号：PCT/US2011/058854
申请日：2011-11-01
申请人： NESS, Kevin, D. , HIDDESSEN, Amy, L. , WYATT, Paul, W.
发明人： NESS, Kevin, D. , HIDDESSEN, Amy, L. , WYATT, Paul, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2527/146 , C12Q2563/159
摘要： Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof.
摘要翻译：分析基因组DNA的方法可以获得包括靶标的基因组DNA。基因组DNA可能会被片段化以产生片段化的DNA。碎片DNA可以通过液滴发生器产生含有片段DNA的水滴。可以对液滴进行测定以确定靶标的水平。在一些实施方案中，液滴可以含有至少约5纳克每微升浓度的基因组DNA，所述液滴可以以每秒至少约50个液滴的液滴产生频率产生，所述液滴可以具有每滴小于约10纳升，可以以大于约50纳升/秒的流速或其任何组合产生液滴。

7. 发明申请

WO2014145582A2 SILICONE SURFACTANTS FOR EMULSION ASSAYS 审中-公开
标题翻译：用于乳化剂测定的硅氧烷表面活性剂
公开(公告)号：WO2014145582A2
公开(公告)日：2014-09-18
申请号：PCT/US2014030374
申请日：2014-03-17
申请人： BIO RAD LABORATORIES , CHIA ERIN R , HIDDESSEN AMY L , HINDSON BENJAMIN J , LOWE ADAM , HAN CHUNXIAO
发明人： CHIA ERIN R , HIDDESSEN AMY L , HINDSON BENJAMIN J , LOWE ADAM , HAN CHUNXIAO
IPC分类号： C12P7/64
CPC分类号： C07F7/184 , C08G77/46 , C08G77/50 , C08L83/12 , C08L83/14 , G01N33/5436
摘要： System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.
摘要翻译：用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。分析物的测定可以用液滴进行。在一些情况下，可以从液滴中检测信号，并且可以基于信号来确定分析物的特征，例如分析物水平或活性。

8. 发明申请

WO2012061444A2 SYSTEM FOR FORMING EMULSIONS 审中-公开
标题翻译：用于形成乳剂的系统
公开(公告)号：WO2012061444A2
公开(公告)日：2012-05-10
申请号：PCT/US2011058857
申请日：2011-11-01
申请人： HIDDESSEN AMY L , MASQUELIER DONALD A , NESS KEVIN D , HINDSON BENJAMIN J , MAKAREWICZ ANTHONY J JR , STEENBLOCK ERIN R
发明人： HIDDESSEN AMY L , MASQUELIER DONALD A , NESS KEVIN D , HINDSON BENJAMIN J , MAKAREWICZ ANTHONY J JR , STEENBLOCK ERIN R
IPC分类号： F17D1/16 , F03B11/02
CPC分类号： B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F13/1022 , B01L3/502761 , B01L2200/0605 , B01L2200/0673 , B01L2300/0867 , B01L2400/0487 , G01N1/38 , G01N2035/1046 , Y10T137/0396 , Y10T137/8593
摘要： System, including methods, apparatus, and kits, for forming emulsions. The system may include an instrument and a microfluidic chip received by the instrument. The instrument may apply pressure to prospective emulsion phases held by the chip, to drive formation and collection of emulsions in the chip. In some embodiments, the instrument may stop applying pressure to the chip when a change in pressure meeting a predefined condition is detected by the instrument. The change may indicate that an endpoint of droplet generation has been reached.
摘要翻译：系统，包括用于形成乳液的方法，设备和试剂盒。该系统可以包括由仪器接收的仪器和微流体芯片。该仪器可以对由芯片保持的预期乳剂阶段施加压力，以驱动芯片中乳剂的形成和收集。在一些实施例中，当仪器检测到满足预定条件的压力变化时，仪器可以停止对芯片施加压力。改变可能表明已经达到了液滴产生的终点。

9. 发明申请

WO2011120020A1 DROPLET TRANSPORT SYSTEM FOR DETECTION 审中-公开
标题翻译：用于检测的DROPLET运输系统
公开(公告)号：WO2011120020A1
公开(公告)日：2011-09-29
申请号：PCT/US2011/030097
申请日：2011-03-25
申请人： QUANTALIFE, INC. , NESS, Kevin, D. , HINDSON, Benjamin, J. , MAKAREWICZ, Anthony, J. , HIDDESSEN, Amy, L.
发明人： NESS, Kevin, D. , HINDSON, Benjamin, J. , MAKAREWICZ, Anthony, J. , HIDDESSEN, Amy, L.
IPC分类号： C12Q1/68 , B01D57/00
CPC分类号： B01L3/021 , B01L2200/0673 , B01L2400/0478 , B01L2400/0622
摘要： System, including methods and apparatus, for transporting droplets from a tip to an examination site for detection.
摘要翻译：用于将液滴从尖端输送到检查部位以进行检测的系统，包括方法和装置。

10. 发明申请

WO2011109546A1 EMULSION CHEMISTRY AND ASSAYS FOR ENCAPSULATED DROPLETS 审中-公开
标题翻译：乳化剂化学和测定用于封堵的药物
公开(公告)号：WO2011109546A1
公开(公告)日：2011-09-09
申请号：PCT/US2011/026901
申请日：2011-03-02
申请人： QUANTALIFE, INC. , HIDDESSEN, Amy, L. , HINDSON, Benjamin, J.
发明人： HIDDESSEN, Amy, L. , HINDSON, Benjamin, J.
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： C12Q1/6848 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , C12Q2527/125 , C12Q2563/159
摘要： System, including methods, apparatus, compositions, and kits, for making and using stabilized emulsions and for assays with an emulsion including capsules. In an exemplary method, an aqueous phase may be provided. The aqueous phase may include a sample and an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of the aqueous phase disposed in a nonaqueous continuous phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules. Assay data related to the sample may be collected from the capsules.
摘要翻译：用于制备和使用稳定化乳剂的方法，装置，组合物和试剂盒以及用包含胶囊的乳液测定的系统。在一个示例性方法中，可以提供水相。水相可以包括样品和有效浓度的一种或多种皮肤形成蛋白质。可以形成乳液。乳液可以包括设置在非水连续相中的水相的液滴。可以加热乳液以在每个液滴和连续相之间产生界面皮肤，以将液滴转化成胶囊。可以从胶囊中收集与样品相关的测定数据。

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