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1. 发明申请

WO2014104880A1 ECONOMICAL PROCESS FOR THE ISOLATION OF FUNCTIONAL PROTEIN FROM PLANTS 审中-公开
标题翻译：从植物中分离功能蛋白的经济过程
公开(公告)号：WO2014104880A1
公开(公告)日：2014-07-03
申请号：PCT/NL2013/050943
申请日：2013-12-20
申请人： NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO
发明人： DE JONG, Govardus, Adrianus, Hubertus , GEERDINK, Peter , BUSSMANN, Paulus, Josephus, Theodorus , HYLKEMA, Nienke
IPC分类号： A23L1/30 , A23J1/00
CPC分类号： A23J1/006 , A23J1/007 , A23J3/14 , A23J3/22 , A23L29/10 , A23L33/105 , A23L33/185 , A23P30/40 , A23V2002/00 , B01D11/00 , B01D15/08 , B01D61/145 , B01D61/147 , C12N9/88 , C12Y401/01039 , A23V2250/548 , A23V2300/10 , A23V2300/16 , A23V2300/30 , A23V2300/34
摘要： The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.
摘要翻译：本发明涉及从植物材料获得蛋白质的方法，其中该方法包括以下步骤：i）在还原剂存在下机械破坏植物细胞以获得植物汁; ii）处理植物汁引起叶绿体膜的聚集，iii）通过沉淀和/或微过滤除去聚集的叶绿体膜，iv）使植物汁超滤，和v）使可溶性植物蛋白浓缩物经受疏水柱吸附以除去残留的叶绿素，酚类化合物和脱落在单列通道中。本发明还涉及基于该方法的用于植物蛋白分离的装置和系统。分离的蛋白质可以以批量级和大规模经济地获得。此外，本发明涉及通过本发明的方法获得的蛋白质，包含其的食品及其用途。

2. 发明申请

WO1998004146A1 FROZEN FOOD WITH ANTIFREEZE PEPTIDES 审中-公开
标题翻译：冷冻食品与抗冻肽
公开(公告)号：WO1998004146A1
公开(公告)日：1998-02-05
申请号：PCT/EP1997003635
申请日：1997-07-04
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： UNILEVER N.V. , UNILEVER PLC , FENN, Richard, Anthony , NEEDHAM, David , SMALLWOOD, Keith
IPC分类号： A23G09/02
CPC分类号： A23G9/38 , A23G9/42 , A23G9/48 , A23G9/52 , A23G2200/10 , A23J1/007 , A23L3/37 , C07K14/415 , C12N15/8273
摘要： A process for the production of a frozen food product comprising AFP, wherein the conditions are chosen such that the ice-crystals in the product have an aspect ratio of from 1.9 to 3.
摘要翻译：一种制备包含AFP的冷冻食品的方法，其中选择条件使得产品中的冰晶具有1.9至3的纵横比。

3. 发明申请

WO1982003158A1 IMPROVED COAGULATION METHOD FOR PREPARING LEAF PROTEIN CONCENTRATES(LPC)FROM PLANT FOLIAGE 审中-公开
标题翻译：用于从植物油中制备叶绿素浓度（LPC）的改进的凝聚方法
公开(公告)号：WO1982003158A1
公开(公告)日：1982-09-30
申请号：PCT/US1982000262
申请日：1982-03-04
申请人： INT HARVESTER CO
发明人： INT HARVESTER CO , DEJONG DONALD W
IPC分类号： A23J01/14
CPC分类号： A23J1/007 , Y10S426/807
摘要： L'adjonction d'un reactif multifonctionnel de reticulation, tel qu'un glutaraldehyde, a du jus vert tout en abaissant le pH jusqu'a une valeur comprise dans la gamme de 3,0 a 5,0, par exemple par l'adjonction de bisulphate de potassium, produit une precipitation sensiblement complete des proteines solubles a partir du jus vert extrait des tissus des plantes feuillues et coagule simultanement la proteine en suspension. Le reactif multifonctionnel de reticulation peut etre en general tout compose possedant une structure chimique comprise dans la formule generale suivante: (FORMULE) ou X est un ion divalent tel que de l'oxygene, du soufre ou du NH; R est un hydrogene ou un radical alkylique a chaine courte possedant de 1 a 5 atomes de carbone; n est un nombre entier compris dans la gamme de 0 a 10.

4. 发明申请

WO2018232008A1 RUBISCO PROTEIN FIBERS 审中-公开
公开(公告)号：WO2018232008A1
公开(公告)日：2018-12-20
申请号：PCT/US2018/037367
申请日：2018-06-13
申请人： R.J. REYNOLDS TOBACCO COMPANY
发明人： SEBASTIAN, Andries Don , MUA, John Paul , MCCLANAHAN, David Neil , JACKSON, Thaddeus J. , ENI ENI, Sammy
IPC分类号： A24B15/12 , A24B15/16 , A24B15/24 , A23J1/00 , A23J3/14 , A23J3/26 , A23J3/34
CPC分类号： C12N9/88 , A23J1/007 , A23J3/14 , A23J3/26 , A23J3/34 , A24B15/12 , A24B15/16 , A24B15/24 , A24B15/241 , C12Y401/01039
摘要： Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C, filtering the heated combination through an about 20μm filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

5. 发明申请

WO2014195975A3 PLANT FRACTIONS HAVING ANTI-PATHOGENESIS PROPERTIES. 审中-公开
标题翻译：具有抗病原性的植物部分。
公开(公告)号：WO2014195975A3
公开(公告)日：2015-02-19
申请号：PCT/IN2014000377
申请日：2014-06-03
申请人： SAVANGIKAR CHITRA VASANT , SAVANGIKA VASANT ANANTRAO
发明人： SAVANGIKAR CHITRA VASANT , SAVANGIKA VASANT ANANTRAO
IPC分类号： A61K36/00 , A61K36/42 , A61K36/48 , A61K36/73 , C07K14/10 , C07K14/415
CPC分类号： A61K38/168 , A23J1/006 , A23J1/007 , A23L2/02 , A23L33/185 , A61K36/48 , A61K45/06
摘要： This invention comprises a composition comprising heat stable plant juice soluble plant proteins and a method of using the same for anti-pathogenesis effect. The said composition is standardized for a parameter of a property of the composition that is relatable to anti-pathogenesis property or to the content of the protein molecules having molecular weight of 26 kilo Daltons (kD) or less. The said anti-pathogenesis effect comprises an anti-microbial effect on living beings or as an Adiponectin agonist effect in mammals. The Adiponectin agonist effect in mammals comprises reducing the risk of one or more of a disorder selected from Type II diabetes, hyperlipidemia, atherosclerosis, hypertension, heart disease and obesity. The anti¬ microbial effect comprises reducing the risk of or treating the microbial infections in plants and animals, with or without synergistic interaction with other antimicrobial agents. This invention also comprises a plant material composition not containing, heat stable proteins; wherein the composition is made by pulping the plant material, removing juice from the solid residue, heating juice to remove coagulable protein and non-protein matter as a coagulum, removing the coagulum and thereafter removing the heat stable proteins from the resulting composition, optionally treating the same to get a further composition comprises either non-protein hydrophilic plant juice solubles or non-protein lipophilic plant juice solubles or both together in same composition.
摘要翻译：本发明包含含有热稳定植物汁可溶性植物蛋白的组合物及使用其的抗病理作用的方法。所述组合物针对与抗病理特性相关的组合物性质的参数或与分子量为26千道尔顿（kD）或更低的蛋白质分子的含量相关的参数进行标准化。所述抗发病作用包括对生物体的抗微生物作用或在哺乳动物中作为脂联素激动剂作用。哺乳动物中的脂联素激动剂作用包括降低选自II型糖尿病，高脂血症，动脉粥样硬化，高血压，心脏病和肥胖的一种或多种病症的风险。抗微生物作用包括降低或治疗植物和动物中微生物感染的风险，有或没有与其他抗微生物剂的协同相互作用。本发明还包含不含热稳定蛋白质的植物材料组合物; 其中所述组合物通过制浆植物材料，从固体残余物除去汁液，加热汁液以除去凝结物中的可凝结蛋白质和非蛋白质物质，除去凝结物，然后从所得组合物中除去热稳定蛋白质，任选地处理获得进一步组合物的组合物包含相同组合物中的非蛋白质亲水植物汁液可溶物或非蛋白质亲脂植物汁液可溶物或两者。

6. 发明申请

WO2011078671A1 PROCESS FOR ISOLATING A DECHLOROPHYLLLIZED RUBISCO PREPARATION FROM A PLANT MATERIAL 审中-公开
标题翻译：用于从植物材料中分离出去乙烯基化的稀土制备方法
公开(公告)号：WO2011078671A1
公开(公告)日：2011-06-30
申请号：PCT/NL2010/050881
申请日：2010-12-22
申请人： NIZO FOOD RESEARCH B.V. , VAN DE VELDE, Fred , ALTING, Aart Cornelis , POUVREAU, Laurice
发明人： VAN DE VELDE, Fred , ALTING, Aart Cornelis , POUVREAU, Laurice
IPC分类号： C12N9/88
CPC分类号： A23J1/007 , A23L33/105 , C12N9/88
摘要： The invention relates a process for the isolation of RuBisCO (ribulose-1,5-bisphosphate carboxylase oxygenase) from a plant material said process comprising: a.lysing the plant material to release RuBisCO and chlorophyll from the plant cells; b.separating the lysed plant material into a liquid juice and a high solids slurry or a high solids cake, said liquid juice containing RuBisCO as well as chlorophyll; c.contacting the liquid juice with activated carbon to adsorb chlorophyll onto the activated carbon; d.separating the dechlorophyllized liquid juice from the chlorophyll-loaded activated carbon; and e.further processing the dechlorophyllized liquid juice to produce the dechlorophyllized RuBisCO preparation. This present process yields a protein preparation that is essentially free of chlorophyll and in which the bulk of the RuBisCO is undenatured. The invention further provides a particulate dechlorophyllized RuBisCO preparation that is obtained by the aforementioned process, said preparation having a weight averaged diameter of 10-1000 µm; containing at least 30 wt.% of undenatured RuBisCO and containing less than 0.1% of chlorophyll by weight of RuBisCO.
摘要翻译：本发明涉及从植物材料分离RuBisCO（核酮糖-1,5-二磷酸羧化酶加氧酶）的方法，所述方法包括：a。将植物材料从植物细胞中释放出RuBisCO和叶绿素; b将裂解的植物材料分离成液体汁和高固体浆料或高固体饼，所述液体汁含有RuBisCO以及叶绿素; c。用液体汁与活性炭接触以将叶绿素吸附到活性炭上; 将叶绿素化的液体汁与载有叶绿素的活性炭分离; 并且e进一步处理脱叶酸液体汁以产生脱叶酸RuBISCO制剂。本发明方法产生基本上不含叶绿素的蛋白质制剂，其中大部分RuBisCO未变性。本发明还提供通过上述方法得到的颗粒状脱叶酸RuBisCO制剂，所述制剂的重均粒径为10-1000μm; 含有至少30重量％的未变性RuBisCO并且含有少于0.1重量％的RuBisCO的叶绿素。

7. 发明申请

WO2005117602A1 METHOD AND SYSTEM FOR SOLUBILIZING PROTEIN 审中-公开
标题翻译：用于溶解蛋白质的方法和系统
公开(公告)号：WO2005117602A1
公开(公告)日：2005-12-15
申请号：PCT/US2005/019497
申请日：2005-06-01
申请人： THE TEXAS A & M UNIVERSITY SYSTEM , HOLTZAPPLE, Mark, T. , DAVISON, Richard , GRANDA, Cesar , NOYES, Gary, P.
发明人： HOLTZAPPLE, Mark, T. , DAVISON, Richard , GRANDA, Cesar , NOYES, Gary, P.
IPC分类号： A23J1/00
CPC分类号： A23J1/002 , A23J1/005 , A23J1/007 , A23J1/10 , A23J3/04 , A23J3/14 , A23J3/32 , A23K10/22 , A23K10/26 , A23K10/30 , A23K20/147 , A23K50/10 , A23V2002/00 , A23V2200/238
摘要： A method of solubilizing protein including adding an alkali, such as lime, and heating to produce a reaction liquid then separating solids from the reaction liquid followed by neutralizing the reaction liquid to produce a neutralized liquid. The method then includes concentrating the neutralized liquid and returning water to the reaction. The disclosure also includes systems for conducting such a method.
摘要翻译：一种溶解蛋白质的方法，包括加入碱如石灰，并加热以产生反应液体，然后从反应液中分离固体，然后中和反应液体以产生中和的液体。然后，该方法包括浓缩中和的液体并将水返回到反应中。本公开还包括用于进行这种方法的系统。

8. 发明申请

WO1998004147A1 FROZEN FOOD WITH ANTIFREEZE PEPTIDES 审中-公开
标题翻译：冷冻食品与抗冻肽
公开(公告)号：WO1998004147A1
公开(公告)日：1998-02-05
申请号：PCT/EP1997003636
申请日：1997-07-04
申请人： UNILEVER N.V. , UNILEVER PLC
发明人： UNILEVER N.V. , UNILEVER PLC , FENN, Richard, Anthony , NEEDHAM, David , SMALLWOOD, Keith
IPC分类号： A23G09/02
CPC分类号： A23G9/38 , A23G9/42 , A23G9/48 , A23G9/52 , A23G2200/10 , A23J1/007 , A23L3/37 , C07K14/415 , C12N15/8273
摘要： A process for the production of a frozen food product comprising AFP, wherein the conditions are chosen such that the ice-crystals in the product have an aspect ratio of from 1.1 to 1.9.
摘要翻译：一种制备包含AFP的冷冻食品的方法，其中选择条件使得产品中的冰晶具有1.1至1.9的纵横比。

9. 发明申请

WO1995015377A1 PLANT ARABINOGALACTAN PROTEIN (AGP) GENES 审中-公开
标题翻译：植物阿拉伯糖苷蛋白（AGP）基因
公开(公告)号：WO1995015377A1
公开(公告)日：1995-06-08
申请号：PCT/AU1994000744
申请日：1994-12-01
申请人： ALBRIGHT & WILSON (AUSTRALIA) LIMITED
发明人： ALBRIGHT & WILSON (AUSTRALIA) LIMITED , CHEN, Chao-Guang , MAU, Shaio-Lim , DU, He , GANE, Alison, M. , BACIC, Antony , CLARKE, Adrienne, E.
IPC分类号： C12N15/10
CPC分类号： C07K14/415 , A23J1/007 , A61K38/00 , C12N15/8241
摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia and Pyrus communis. Amino acid sequences of isolated AGP peptide molecules are presented. Isolated AGP molecules were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP molecules were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.
摘要翻译：本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGP。提出了分离的AGP肽分子的氨基酸序列。使用分离的AGP分子来合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。分离编码分离的AGP分子的氨基酸序列的cDNA克隆。本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。

10. 发明申请

WO2014186671A3 TOBACCO-DERIVED PROTEIN COMPOSITIONS 审中-公开
标题翻译：烟草衍生的蛋白质组合物
公开(公告)号：WO2014186671A3
公开(公告)日：2015-03-26
申请号：PCT/US2014038342
申请日：2014-05-16
申请人： REYNOLDS TOBACCO CO R
发明人： GERARDI ANTHONY RICHARD , BYRD CRYSTAL DAWN HEGE , JACKSON THADDEUS JUDE , BETTS CHELSEA ALLISON , MUA JOHN-PAUL , FORD KYLE
IPC分类号： A24B15/24 , A23J1/00 , A23J3/14 , A23K1/16 , A23L1/015 , A23L1/305 , A23L2/66 , A24B15/18 , C07K14/415
CPC分类号： A23J1/007 , A23J3/14 , A23K20/147 , A23L2/66 , A23L5/23 , A23L5/273 , A23L33/185 , A23V2002/00 , A24B15/18 , A24B15/20 , A24B15/24 , A24B15/241 , C07K14/415 , C12N9/88 , C12Y401/01039
摘要： The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and/or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and/or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 µg/g; and a heavy metal content of less than about 60 µg/g.
摘要翻译：本公开描述了用于纯化富含蛋白质的提取物以提供浓缩物和分离物的方法以及将这些材料并入产品中的方法。净化方法适于从提取物中除去灰分，金属盐，生物碱，颗粒，重金属和其它杂质和/或污染物中的一种或多种，以及改变感官特征（例如，气味，颜色和/ 或味道特征）。方法通常包括渗滤，用官能化树脂处理和超临界萃取。提供浓缩物或分离物形式的蛋白质组合物，所述蛋白质组合物包括从烟草属植物植物提取的RuBisCO，F2级分蛋白质或其组合，其中所述蛋白质组合物的特征在于以下一种或多种：灰分含量小于约15重量％; 尼古丁含量小于约10μg/ g; 重金属含量小于约60μg/ g。

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