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1. 发明申请

WO1995015377A1 PLANT ARABINOGALACTAN PROTEIN (AGP) GENES 审中-公开
标题翻译：植物阿拉伯糖苷蛋白（AGP）基因
公开(公告)号：WO1995015377A1
公开(公告)日：1995-06-08
申请号：PCT/AU1994000744
申请日：1994-12-01
申请人： ALBRIGHT & WILSON (AUSTRALIA) LIMITED
发明人： ALBRIGHT & WILSON (AUSTRALIA) LIMITED , CHEN, Chao-Guang , MAU, Shaio-Lim , DU, He , GANE, Alison, M. , BACIC, Antony , CLARKE, Adrienne, E.
IPC分类号： C12N15/10
CPC分类号： C07K14/415 , A23J1/007 , A61K38/00 , C12N15/8241
摘要： This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia and Pyrus communis. Amino acid sequences of isolated AGP peptide molecules are presented. Isolated AGP molecules were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP molecules were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.
摘要翻译：本发明提供植物阿拉伯半乳聚糖蛋白（AGP）及其基因。从Nicotiana alata，Nicotiana plumbaginafolia和Pyrus communis分离AGP。提出了分离的AGP肽分子的氨基酸序列。使用分离的AGP分子来合成寡核苷酸探针以制备用于PCR的寡核苷酸引物或制备RNA探针以筛选N.alata，N.plumbaginafolia和P. communis的cDNA文库。分离编码分离的AGP分子的氨基酸序列的cDNA克隆。本发明首次呈现源自相应AGP基因的完整AGP氨基酸序列。本发明还提供了可用于获得编码AGP肽的AGP基因的方法，所述AGP肽包含特异性分离的富含脯氨酸（富含OAST）（OAST）序列或特异性分离的不存在羟脯氨酸的序列。

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