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    • 3. 发明申请
    • METHOD OF MODULATING FAT TASTE PERCEPTION
    • 调节脂肪变性的方法
    • WO2017030862A1
    • 2017-02-23
    • PCT/US2016/046302
    • 2016-08-10
    • MONELL CHEMICAL SENSES CENTER
    • BACHMANOV, Alexander, A.MATSUMOTO, IchiroOHMOTO, Makoto
    • C07K14/705A23L1/305G01N33/53C12N5/10C12N15/12
    • C07K14/705C12N2510/00G01N33/566G01N2333/726
    • Methods for identifying a compound that mimics or modulates fat taste perception employ screening recombinant cell or cell line-based assays that monitor the effect of a test compound on the function, activity or pathway stimulation of a nucleic acid sequence encoding GPR113, optionally co-expressed with Gna14, with one or more T1R receptors, or with one or more additional G proteins, or with chimeric molecules containing at least a portion of Gna14. A test compound that mimics or modulates the effect of dietary fat causes a change in the physical or functional characteristic of the contacted cells or cell lines in comparison to a control cell. Other cell-free assays that measure the ability of a test compound to disrupt or displace the binding of the GPR113 protein with the Gna14 protein and optionally other proteins, e.g., TIRs, are also useful. Reagents and devices employing such recombinant cells are also disclosed.
    • 用于鉴定模拟或调节脂肪感觉的化合物的方法采用筛选重组细胞或基于细胞系的测定法,其监测测试化合物对编码GPR113的核酸序列的功能,活性或途径刺激的影响,任选共表达 Gna14与一种或多种T1R受体或一种或多种另外的G蛋白,或含有至少一部分Gna14的嵌合分子。 与对照细胞相比,模拟或调节膳食脂肪的作用的测试化合物导致接触的细胞或细胞系的物理或功能特征的变化。 测量测试化合物破坏或置换GPR113蛋白与Gna14蛋白和任选的其它蛋白(例如TIR)的结合的能力的其它无细胞测定也是有用的。 还公开了使用这种重组细胞的试剂和装置。
    • 9. 发明申请
    • PREPARATION OF SOY PROTEIN PRODUCTS (
    • 大豆蛋白产品的制备(“S810”)
    • WO2016029314A1
    • 2016-03-03
    • PCT/CA2015/050819
    • 2015-08-27
    • BURCON NUTRASCIENCE (MB) CORP.
    • SEGALL, Kevin I.SCHWEIZER, MartinGREEN, Brent E.
    • A23J3/16A23J1/14A23L1/211A23L1/305
    • A23J1/14A23L2/66A23L33/185
    • The present invention is directed to soy protein products, very low in, or free of, beany flavour notes and useful for the fortification of food and beverage products and prepared without the use of salt in the process. The soy protein products of the present invention are obtained by extracting soy protein source with water to form an aqueous soy protein solution, at least partially separating the aqueous soy protein solution from residual soy protein source, adjusting the pH of the aqueous soy protein solution to a pH of about 1.5 to about 3.6 to solubilize at least a portion of the protein and form an acidified soy protein solution then separating the acidified soy protein solution from the acid insoluble solid material. The acidified soy protein solution may be dried following optional concentration and diafiltration to form a soy protein product, which may be an isolate. The acid insoluble solid material may be washed with acidified water and then dried to form another soy protein product. These products may be dried at the acidic pH at which they were prepared or may be adjusted in pH before drying.
    • 本发明涉及非常低或不含任何风味香料的大豆蛋白产品,并且可用于强化食品和饮料产品并且在该过程中不使用盐制备。 本发明的大豆蛋白产品是通过用水提取大豆蛋白源而形成大豆蛋白水溶液,通过将大豆蛋白水溶液与残留的大豆蛋白源至少部分分离,将大豆蛋白水溶液的pH调节至 约1.5至约3.6的pH以溶解蛋白质的至少一部分并形成酸化的大豆蛋白溶液,然后将酸化的大豆蛋白溶液与酸不溶性固体材料分离。 酸化的大豆蛋白溶液可以在任选的浓度和渗滤之后干燥以形成大豆蛋白产物,其可以是分离物。 酸不溶性固体物质可用酸化水洗涤,然后干燥以形成另一种大豆蛋白产品。 这些产品可以在其制备的酸性pH下干燥,或者可以在干燥前调节pH。