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1. 发明申请

WO0189788A2 PATTERNING OF SURFACES UTILIZING MICROFLUIDIC STAMPS INCLUDING THREE-DIMENSIONALLY ARRAYED CHANNEL NETWORKS 审中-公开
标题翻译：使用包括三维阵列通道网络在内的微流感标记的表面图案
公开(公告)号：WO0189788A2
公开(公告)日：2001-11-29
申请号：PCT/US0117246
申请日：2001-05-25
申请人： HARVARD COLLEGE , CHILDRENS MEDICAL CENTER , CHIU DANIEL T , JEON NOO LI , HUANG SUI , KANE RAVI , CHOI INSUNG S , INGBER DONALD E , WHITESIDES GEORGE M , ANDERSON JANELLE R
发明人： CHIU DANIEL T , JEON NOO-LI , HUANG SUI , KANE RAVI , CHOI INSUNG S , INGBER DONALD E , WHITESIDES GEORGE M , ANDERSON JANELLE R
IPC分类号： B01F13/00 , B01L3/00 , B01L3/02 , B29C31/04 , B29C33/42 , B29C39/10 , B81B1/00 , B81C1/00 , C40B40/10 , C40B60/14 , G01N33/50 , B29C
CPC分类号： C12N11/00 , B01F13/0059 , B01J2219/00382 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B01J2219/00725 , B01J2219/00743 , B01L3/0258 , B01L3/0262 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0874 , B01L2300/0887 , B29C31/04 , B29C33/424 , B29C39/10 , B33Y80/00 , B81B1/00 , B81C99/009 , B82Y5/00 , B82Y30/00 , C40B40/10 , C40B60/14 , G01N33/5005 , Y10S530/81 , Y10S530/811 , Y10S530/812 , Y10S530/815
摘要： The present invention describes improved microfluidic systems and procedures for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The methods for fabrication the systems disclosed can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks can be fabricated via replica molding processes utilizing mold masters including surfaces having topological features formed by photolithography. The present invention also involves microfluidic systems and methods for fabricating complex patterns of materials, such as biological materials and cells, on surfaces utilizing the microfluidic systems. Specifically, the invention provides microfluidic surface patterning systems and methods for fabricating complex, discontinuous patterns on surfaces that can incorporate or deposit multiple materials onto the surfaces. The present invention also provides improved microfluidic stamps or applicators for microcontact surface patterning, which are able to pattern onto a surface arbitrary two-dimensional patterns, and which are able to pattern multiple substances onto a surface without the need for multiple steps of registration or stamping during patterning and without the need to selectively "ink" different regions of the stamp with different materials.
摘要翻译：本发明描述了改进的微流体系统和用于制造改进的微流体系统的程序，其包含一个或多个水平的微流体通道。用于制造所公开的系统的方法可以为拓扑复杂和改进的微流体系统提供方便的途径。微流体系统可以包括其中的流体流动路径的三维排列的网络，其中包括在交叉点处交叉的网络上或下方的通道，而没有物理交叉点。微流体网络可以通过使用模具主体的复制模制工艺制造，其中包括具有通过光刻形成的拓扑特征的表面。本发明还涉及在利用微流体系统的表面上制造诸如生物材料和细胞的复杂图案的微流体系统和方法。具体地，本发明提供了微流体表面图案化系统和用于在表面上制造复杂的不连续图案的方法，所述图案可以将多种材料结合或沉积到表面上。本发明还提供了用于微接触表面图案化的改进的微流体标记或涂布器，其能够将图案图案化成任意的二维图案，并且能够将多种物质图案化到表面上，而不需要多个对准或冲压的步骤在图案化期间，并且不需要使用不同材料选择性地“印刷”印模的不同区域。

2. 发明申请

WO0189788A9 PATTERNING OF SURFACES UTILIZING MICROFLUIDIC STAMPS INCLUDING THREE-DIMENSIONALLY ARRAYED CHANNEL NETWORKS 审中-公开
标题翻译：使用包括三维阵列通道网络在内的微流感标记的表面图案
公开(公告)号：WO0189788A9
公开(公告)日：2002-12-12
申请号：PCT/US0117246
申请日：2001-05-25
申请人： HARVARD COLLEGE , CHILDRENS MEDICAL CENTER , CHIU DANIEL T , JEON NOO-LI , HUANG SUI , KANE RAVI , CHOI INSUNG S , INGBER DONALD E , WHITESIDES GEORGE M , ANDERSON JANELLE R
发明人： CHIU DANIEL T , JEON NOO-LI , HUANG SUI , KANE RAVI , CHOI INSUNG S , INGBER DONALD E , WHITESIDES GEORGE M , ANDERSON JANELLE R
IPC分类号： B01F13/00 , B01L3/00 , B01L3/02 , B29C31/04 , B29C33/42 , B29C39/10 , B81B1/00 , B81C1/00 , C40B40/10 , C40B60/14 , G01N33/50 , B01J4/00 , B29C39/02
CPC分类号： C12N11/00 , B01F13/0059 , B01J2219/00382 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B01J2219/00725 , B01J2219/00743 , B01L3/0258 , B01L3/0262 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0874 , B01L2300/0887 , B29C31/04 , B29C33/424 , B29C39/10 , B33Y80/00 , B81B1/00 , B81C99/009 , B82Y5/00 , B82Y30/00 , C40B40/10 , C40B60/14 , G01N33/5005 , Y10S530/81 , Y10S530/811 , Y10S530/812 , Y10S530/815
摘要： The present invention describes improved microfluidic systems and procedures for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The methods for fabrication the systems disclosed can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks can be fabricated via replica molding processes utilizing mold masters including surfaces having topological features formed by photolithography. The present invention also involves microfluidic systems and methods for fabricating complex patterns of materials, such as biological materials and cells, on surfaces utilizing the microfluidic systems. Specifically, the invention provides microfluidic surface patterning systems and methods for fabricating complex, discontinuous patterns on surfaces that can incorporate or deposit multiple materials onto the surfaces. The present invention also provides improved microfluidic stamps or applicators for microcontact surface patterning, which are able to pattern onto a surface arbitrary two-dimensional patterns, and which are able to pattern multiple substances onto a surface without the need for multiple steps of registration or stamping during patterning and without the need to selectively "ink" different regions of the stamp with different materials.
摘要翻译：本发明描述了改进的微流体系统和用于制造改进的微流体系统的程序，其包含一个或多个水平的微流体通道。用于制造所公开的系统的方法可以提供拓扑复杂和改进的微流体系统的方便的途径。微流体系统可以包括其中的流体流动路径的三维排列的网络，其中包括在交叉点处交叉的网络上或下方的通道，而没有物理交叉点。微流体网络可以通过使用模具主体的复制模制工艺制造，其中包括具有通过光刻形成的拓扑特征的表面。本发明还涉及在利用微流体系统的表面上制造诸如生物材料和细胞的复杂图案的微流体系统和方法。具体地，本发明提供了微流体表面图案化系统和用于在表面上制造复杂的不连续图案的方法，所述图案可以将多种材料结合或沉积到表面上。本发明还提供了用于微接触表面图案化的改进的微流体标记或涂布器，其能够将图案图案化成任意的二维图案，并且能够将多种物质图案化到表面上，而不需要多个对准或冲压的步骤在图案化期间，并且不需要使用不同材料选择性地“印刷”印模的不同区域。

3. 发明申请

WO0222264A8 METHOD AND APPARATUS FOR GRADIENT GENERATION 审中-公开
标题翻译：梯度产生的方法和装置
公开(公告)号：WO0222264A8
公开(公告)日：2002-07-18
申请号：PCT/US0142195
申请日：2001-09-18
申请人： HARVARD COLLEGE , JEON NOO LI , DERTINGER STEPHAN K W , CHIU DANIEL T , CHOI INSUNG S , WHITESIDES GEORGE M
发明人： JEON NOO LI , DERTINGER STEPHAN K W , CHIU DANIEL T , CHOI INSUNG S , WHITESIDES GEORGE M
IPC分类号： G01N33/53 , B01D3/00 , B01F5/06 , B01F13/00 , B01F15/04 , B01J4/00 , B01J19/00 , B01J19/24 , B01L20060101 , B01L1/00 , B01L3/00 , B65B3/00 , B65B3/04 , C12M1/34 , C23G1/00 , G01F11/28 , G01N1/28 , G01N1/38 , G01N33/543 , G01N37/00 , B01F4
CPC分类号： B01F5/0601 , B01F5/064 , B01F13/0059 , B01F15/0404 , B01J4/001 , B01J19/0093 , B01J2219/0086 , B01J2219/00889 , B01L3/5027 , G01N1/2813 , G01N1/38

4. 发明申请

WO0189787A9 MICROFLUIDIC SYSTEMS INCLUDING THREE-DIMENSIONALLY ARRAYED CHANNEL NETWORKS 审中-公开
标题翻译：微型系统，包括三维阵列通道网络
公开(公告)号：WO0189787A9
公开(公告)日：2003-02-06
申请号：PCT/US0116973
申请日：2001-05-25
申请人： HARVARD COLLEGE , ANDERSON JANELLE R , CHIU DANIEL T , JACKMAN REBECCA J , CHERNIAVSKAYA OKSANA , MCDONALD COOPER , WHITESIDES GEORGE M
发明人： ANDERSON JANELLE R , CHIU DANIEL T , JACKMAN REBECCA J , CHERNIAVSKAYA OKSANA , MCDONALD COOPER , WHITESIDES GEORGE M
IPC分类号： G01N37/00 , B01F13/00 , B01L3/00 , B01L3/02 , B29C31/04 , B29C33/40 , B29C33/42 , B29C39/02 , B29C39/24 , B29C65/00 , B81B1/00 , B81C1/00 , B82B3/00 , C12M1/34 , B01J4/00
CPC分类号： B01L3/0258 , B01F13/0059 , B01L3/0262 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0874 , B01L2300/0887 , B29C31/04 , B29C33/405 , B29C33/424 , B29C39/02 , B29C39/24 , B29C66/7373 , B33Y80/00 , B81B2201/058 , B81C1/00119 , B81C2201/034 , B81C2201/036 , B82Y30/00 , Y10T137/2191 , Y10T137/2224 , Y10T436/2575
摘要： The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography. The microfluidic networks of the invention are, in some cases, comprised of a single replica molded layer, and, in other cases, are comprised of two, three, or more replica molded layers that have been assembled to form the overall microfluidic network structure. The present invention also describes various novel applications for using the microfluidic network structures provided by the invention.
摘要翻译：本发明在某些实施方案中提供了改进的微流体系统和用于制造包含一个或多个微流体通道水平的改进的微流体系统的方法。本发明的方法可以提供拓扑复杂和改进的微流体系统的方便的途径。根据本发明提供的微流体系统可以包括其中流体流动路径的三维排列的网络，其中包括跨过网络的其它通道的通道，而在交叉点处没有物理交叉点。本发明的微流体网络可以通过本发明提供的复制模制工艺，利用模具主体，其包括具有通过光刻形成的拓扑特征的表面。在一些情况下，本发明的微流体网络由单个复制模制层组成，并且在其它情况下由两个，三个或更多个复制模制层组成，以形成整个微流体网络结构。本发明还描述了使用本发明提供的微流体网络结构的各种新颖应用。

5. 发明申请

WO0189787A3 MICROFLUIDIC SYSTEMS INCLUDING THREE-DIMENSIONALLY ARRAYED CHANNEL NETWORKS 审中-公开
标题翻译：微型系统，包括三维阵列通道网络
公开(公告)号：WO0189787A3
公开(公告)日：2002-05-30
申请号：PCT/US0116973
申请日：2001-05-25
申请人： HARVARD COLLEGE , ANDERSON JANELLE R , CHIU DANIEL T , JACKMAN REBECCA J , CHERNIAVSKAYA OKSANA , MCDONALD COOPER , WHITESIDES GEORGE M
发明人： ANDERSON JANELLE R , CHIU DANIEL T , JACKMAN REBECCA J , CHERNIAVSKAYA OKSANA , MCDONALD COOPER , WHITESIDES GEORGE M
IPC分类号： G01N37/00 , B01F13/00 , B01L3/00 , B01L3/02 , B29C31/04 , B29C33/40 , B29C33/42 , B29C39/02 , B29C39/24 , B29C65/00 , B81B1/00 , B81C1/00 , B82B3/00 , C12M1/34 , B01J4/00
CPC分类号： B01L3/0258 , B01F13/0059 , B01L3/0262 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0874 , B01L2300/0887 , B29C31/04 , B29C33/405 , B29C33/424 , B29C39/02 , B29C39/24 , B29C66/7373 , B33Y80/00 , B81B2201/058 , B81C1/00119 , B81C2201/034 , B81C2201/036 , B82Y30/00 , Y10T137/2191 , Y10T137/2224 , Y10T436/2575
摘要： The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography. The microfluidic networks of the invention are, in some cases, comprised of a single replica molded layer, and, in other cases, are comprised of two, three, or more replica molded layers that have been assembled to form the overall microfluidic network structure. The present invention also describes various novel applications for using the microfluidic network structures provided by the invention.
摘要翻译：在某些实施方案中，本发明提供了改进的微流体系统和用于制备包含一个或多个微流体通道水平的改进的微流体系统的方法。本发明的方法可以提供拓扑复杂和改进的微流体系统的方便的途径。根据本发明提供的微流体系统可以包括其中流体流动路径的三维排列的网络，其中包括跨过网络的其它通道的通道，而在交叉点处没有物理交叉点。本发明的微流体网络可以通过本发明提供的复制模制工艺，利用模具主体，其包括具有通过光刻形成的拓扑特征的表面。在一些情况下，本发明的微流体网络由单个复制模制层组成，并且在其它情况下由两个，三个或更多个复制模制层组成，以形成整个微流体网络结构。本发明还描述了使用本发明提供的微流体网络结构的各种新颖应用。

6. 发明申请

WO2012100198A3 METHODS AND SYSTEMS FOR PERFORMING DIGITAL MEASUREMENTS 审中-公开
标题翻译：执行数字测量的方法和系统
公开(公告)号：WO2012100198A3
公开(公告)日：2012-10-04
申请号：PCT/US2012022081
申请日：2012-01-20
申请人： UNIV WASHINGTON CT COMMERCIALI , CHIU DANIEL T , FUJIMOTO BRYANT S , GANSEN ALEXANDER R , YEN GLORIA S , LORENZ ROBERT M
发明人： CHIU DANIEL T , FUJIMOTO BRYANT S , GANSEN ALEXANDER R , YEN GLORIA S , LORENZ ROBERT M
IPC分类号： G01F22/00 , C12M1/34 , C12Q1/68 , G01N33/68
CPC分类号： C12Q1/6851 , C12Q1/6806 , C12Q2527/146 , C12Q2531/113 , C12Q2545/114
摘要： Methods, devices and systems for performing digital measurements are provided.
摘要翻译：提供了用于执行数字测量的方法，设备和系统。

7. 发明申请

WO2012054525A3 CHROMOPHORIC POLYMER DOTS 审中-公开
标题翻译：发色聚合物部分
公开(公告)号：WO2012054525A3
公开(公告)日：2012-08-16
申请号：PCT/US2011056768
申请日：2011-10-18
申请人： UNIV WASHINGTON CT COMMERCIALIZATION , CHIU DANIEL T , WU CHANGFENG , ZHANG XUANJUN , YU JIANGBO , YE FANGMAO
发明人： CHIU DANIEL T , WU CHANGFENG , ZHANG XUANJUN , YU JIANGBO , YE FANGMAO
IPC分类号： B82B1/00 , B82B3/00 , B82Y40/00
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , B82Y40/00 , C08G61/126 , C08G2261/12 , C08G2261/1426 , C08G2261/228 , C08G2261/3142 , C08G2261/3246 , C08G2261/522 , C08G2261/54 , C08G2261/72 , C08G2261/94 , C08J3/11 , C08J2327/22 , C09K11/025 , C09K11/06 , C09K2211/1425 , H01L51/0039
摘要： The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.
摘要翻译：除其他方面外，本发明提供了稳定的发色纳米粒子。在某些实施方案中，本文提供的发色纳米粒子用预定数量的官能团合理官能化。在某些实施方案中，本文提供的稳定的发色纳米粒子用低密度的官能团进行修饰。在其他实施方案中，本文提供的发色纳米粒子与一个或多个分子缀合。本文还提供了用于制造合理官能化的发色纳米粒子的方法。

8. 发明申请

WO2011057295A3 FUNCTIONALIZED CHROMOPHORIC POLYMER DOTS AND BIOCONJUGATES THEREOF 审中-公开
标题翻译：功能化的发色聚合物块及其生物复合材料
公开(公告)号：WO2011057295A3
公开(公告)日：2011-09-22
申请号：PCT/US2010056079
申请日：2010-11-09
申请人： UNIV WASHINGTON CT COMMERCIALIZATION , UNIV CLEMSON , CHIU DANIEL T , WU CHANGFENG , MCNEILL JASON , YU JIANGBO
发明人： CHIU DANIEL T , WU CHANGFENG , MCNEILL JASON , YU JIANGBO
IPC分类号： C09K11/02 , C09K11/06 , G01N21/64 , G01N21/76 , G03C1/00
CPC分类号： G01N33/587 , C09K11/06 , G01N33/582
摘要： The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
摘要翻译：除其它方面外，本发明提供了包含疏水核心和亲水性帽的官能化发色聚合物点及其生物缀合物。还提供了用于制备官能化发色聚合物点的改进方法。还公开了用于体内成像和分子标记的方法。

9. 发明申请

WO2010019388A3 METHOD AND APPARATUS FOR THE DISCRETIZATION AND MANIPULATION OF SAMPLE VOLUMES 审中-公开
标题翻译：用于样品体积的分离和操作的方法和设备
公开(公告)号：WO2010019388A3
公开(公告)日：2010-05-14
申请号：PCT/US2009052299
申请日：2009-07-30
申请人： UNIV WASHINGTON , CHIU DANIEL T , COHEN DAWN E , JEFFRIES GAVIN D M
发明人： CHIU DANIEL T , COHEN DAWN E , JEFFRIES GAVIN D M
IPC分类号： G01N33/48 , G01N35/00
CPC分类号： B01L3/502 , B01L3/502784 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , B01L2300/0877 , B01L2300/0883 , B01L2300/165 , B01L2300/1827 , B01L2300/1861 , B01L2400/0439 , B01L2400/0633 , G01N35/08 , G01N35/10 , G01N2035/00356 , G01N2035/1032 , G01N2035/1034
摘要： Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The isolation of the discretized volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements. The minimal fluid interconnects and simple flow geometry makes the device easy to use and implement, economical to fabricate and operate, and robust in its operations.
摘要翻译：本发明的实施例涉及用于对样本体积进行离散化和操纵的方法和装置，其简单，稳健且通用。它是一种流体装置，它通过利用流体力，界面张力，通道几何形状以及形成的液滴和/或离散体积的最终稳定性之间的相互作用来分隔样品。这些划分的体积允许分离样品并划分为局部阵列，随后可以进行操作和分析。离散化体积的分离以及设备的固有可移植性使得我们的发明多用于许多领域，包括但不限于PCR，数字PCR，用于诊断和预测的生物测定，癌症诊断和预后，高通量筛选，单分子和单细胞反应或测定，研究结晶和其他统计过程，蛋白质结晶，药物筛选，环境测试以及与生物医学分析和测量的各种分析检测技术的耦合。最小的流体互连和简单的流动几何形状使得该设备易于使用和实施，制造和操作经济并且在其操作中稳健。

10. 发明申请

WO2004034436A3 METHOD FOR INTERFACING MACROSCALE COMPONENTS TO MICROSCALE DEVICES 审中-公开
标题翻译：用于将微孔组件接合到微结构设备的方法
公开(公告)号：WO2004034436A3
公开(公告)日：2005-03-31
申请号：PCT/US0332151
申请日：2003-10-08
申请人： CELLECTRICON AB , KARLSSON MATTIAS , ORWAR OWE , CHIU DANIEL T
发明人： KARLSSON MATTIAS , ORWAR OWE , CHIU DANIEL T
IPC分类号： B01J19/00 , B01L3/00 , B01L9/00 , F15C1/06 , C09J7/02
CPC分类号： B01J19/0093 , B01J2219/0081 , B01J2219/00891 , B01J2219/0093 , B01J2219/00952 , B01L3/5025 , B01L3/502715 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2300/0829 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487
摘要： The invention provides integrated systems comprising macroscale devices interfaced with microscale devices and methods for making these systems.
摘要翻译：本发明提供了包括与微型装置接口的宏观装置和用于制造这些系统的方法的集成系统。

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