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1. 发明申请

WO2010144746A3 MICROORGANISMS AND METHODS FOR CARBON-EFFICIENT BIOSYNTHESIS OF MEK AND 2-BUTANOL 审中-公开
标题翻译：用于碳氢化合物和2-丁醇的高效生物合成的微生物和方法
公开(公告)号：WO2010144746A3
公开(公告)日：2011-02-24
申请号：PCT/US2010038230
申请日：2010-06-10
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , NIU WEI , BURGARD ANTHONY P
发明人： OSTERHOUT ROBIN E , NIU WEI , BURGARD ANTHONY P
IPC分类号： C12P7/26 , C12P7/16
CPC分类号： C12P7/26 , C12N15/52 , C12P7/16 , Y02E50/10
摘要： A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.
摘要翻译：非天然存在的微生物有至少一种编码MEK途径酶的外源核酸，其表达量足以产生MEK。 MEK途径包括选自乙酰乙酰辅酶A脱氢酶（双功能），乙酰乙酰辅酶A醛脱氢酶，3-氧代丁醛还原酶，3-氧代丁醇脱水酶，MEK氧化还原酶，3-氧代丁醛氨基转移酶，4-氨基丁酸 -2-酮脱氨酶，2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP氨基转移酶，2,4-二氧代戊酸脱羧酶，AKP脱氨酶，乙酰丙酸酯脱羧酶，AKP脱羧酶，谷氨酸脱氢酶，氧化丁醛氧化还原酶（胺化）和AKP氧化还原酶（胺化）。 2-丁醇途径还包括MEK还原酶。包括生产MEK或2-丁醇的方法包括在条件和足够的时间内培养这些生物体以产生MEK或2-丁醇。

2. 发明申请

WO2010141920A3 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物及相关方法
公开(公告)号：WO2010141920A3
公开(公告)日：2011-01-27
申请号：PCT/US2010037544
申请日：2010-06-04
申请人： GENOMATICA INC , VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
发明人： VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
IPC分类号： C12P7/16
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化BDO的表达。本发明还提供了使用这种微生物生产BDO的方法。

3. 发明申请

WO2011140171A3 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF BUTADIENE 审中-公开
标题翻译：二苯乙烯生物合成的微生物和方法
公开(公告)号：WO2011140171A3
公开(公告)日：2012-04-26
申请号：PCT/US2011035105
申请日：2011-05-04
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P5/02 , C12N1/20
CPC分类号： C12P5/026 , C07C11/16 , C08F136/06 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
摘要翻译：本发明提供具有丁二烯途径的非天然存在的微生物。本发明另外提供了使用这种生物体来生产丁二烯的方法。

4. 发明申请

WO2010030711A3 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物
公开(公告)号：WO2010030711A3
公开(公告)日：2010-10-07
申请号：PCT/US2009056415
申请日：2009-09-09
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN
IPC分类号： C12P7/16 , C12P1/04
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/1096 , C12N9/12 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/70 , C12N15/81 , C12Y101/01157 , C12Y402/01055
摘要： The invention provides non- naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量产生BDO的BDO途径酶的外源核酸。本发明还提供了使用这种微生物生产BDO的方法。

5. 发明申请

WO2012177619A3 MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产1,3-丁二醇的微生物及其相关方法
公开(公告)号：WO2012177619A3
公开(公告)日：2014-05-01
申请号：PCT/US2012043117
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P1/00 , C12N1/00 , C12N15/70 , C12N15/74
CPC分类号： C12P7/18 , C12N15/52
摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3 -BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3 -BDO pathway enzyme expressed in a sufficient amount to produce 1,3 -BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：本文提供了具有1,3-丁二醇（1,3-BOD）途径的非天然存在的微生物生物，并且包含至少一种编码1,3 -BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3 -BDO。在一些实施方案中，该途径包括从CO或氢中还原当量。在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

6. 发明申请

WO2009135074A2 MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 审中-公开
标题翻译：用于生产甲基丙烯酸的微生物
公开(公告)号：WO2009135074A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042426
申请日：2009-04-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00 , C12P7/40
CPC分类号： C12P7/42 , C12N9/0008 , C12N9/1096 , C12N9/88 , C12N9/90 , C12P7/40 , C12Y102/01 , C12Y206/01022 , C12Y403/01 , C12Y504/99002
摘要： The invention provides a non-naturally occurring microbial organism having a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3- hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
摘要翻译：本发明提供了具有2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径的非天然存在的微生物生物。微生物生物体含有编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径中的酶的至少一种外源核酸。本发明还提供了2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸的制备方法。该方法可以包括培养足够量的表达至少一种编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径酶的外源核酸的2-羟基异丁酸，3-羟基异丁酸或产生甲基丙烯酸的微生物，并培养在条件下和足够的时间内产生2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸。

7. 发明申请

WO2012177601A3 MICROORGANISMS FOR PRODUCING ISOBUTANOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产ISOBUTANOL的微生物及其相关方法
公开(公告)号：WO2012177601A3
公开(公告)日：2014-05-08
申请号：PCT/US2012043091
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C07C29/00 , C12N1/00 , C12N1/20 , C12P7/16
CPC分类号： C12P7/16 , C12N15/52 , Y02E50/10
摘要： Provided herein is a non-naturally occurring microbial organism having an isopropanol pathway and comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. In certain embodiments, the pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. Also provided herein is anon-naturally occurring microbial organism having an n-butanol pathway and comprising at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism having n-butanol or isobutanol pathways are also provided herein. In certain embodiments, isobutanol pathways utilizing reverse TCA and/or reducing equivalents from CO and/or hydrogen are used to enhance product yields. The organisms provided herein can be cultured to produce isopropanol, n-butanol, or isobutanol.
摘要翻译：本文提供了具有异丙醇途径并且包含至少一种编码异丙醇途径酶的外源核酸的非天然存在的微生物生物。在某些实施方案中，该途径包括选自4-羟基丁酰-CoA脱水酶，巴豆酸酶，3-羟基丁酰辅酶A脱氢酶，乙酰乙酰辅酶A合成酶，乙酰辅酶A：乙酰乙酸酯-CoA转移酶，乙酰乙酰辅酶A 水解酶，乙酰乙酸脱羧酶和丙酮还原酶。本文还提供了具有正丁醇途径的非天然存在的微生物生物，并且包含至少一种编码正丁醇途径酶的外源核酸。本文还提供了其它具有正丁醇或异丁醇途径的非天然存在的微生物。在某些实施方案中，使用利用反向TCA和/或来自CO和/或氢的还原当量的异丁醇途径来提高产物产率。本文提供的生物体可以培养以产生异丙醇，正丁醇或异丁醇。

8. 发明申请

WO2012135789A3 MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO 审中-公开
标题翻译：生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法
公开(公告)号：WO2012135789A3
公开(公告)日：2014-05-01
申请号：PCT/US2012031733
申请日：2012-03-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00
CPC分类号： C12P7/40 , C12P7/42 , C12P7/62 , C12P19/32
摘要： The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
摘要翻译：本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

9. 发明申请

WO2013028519A4 MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS 审中-公开
标题翻译：用于生产2,4-二戊二酸，二丁烯，丙烯，1,3-丁二醇和相关醇的微生物和方法
公开(公告)号：WO2013028519A4
公开(公告)日：2013-05-10
申请号：PCT/US2012051347
申请日：2012-08-17
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , BURGARD ANTHONY P , BURK MARK J
发明人： OSTERHOUT ROBIN E , BURGARD ANTHONY P , BURK MARK J
IPC分类号： C12P7/18
CPC分类号： C12P7/16 , C12N15/70 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/40 , Y02E50/10
摘要： The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non- naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.
摘要翻译：本发明提供非天然存在的微生物，其含有2,4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径，其包含至少一种编码丁二烯途径酶的外源核酸，足量生产2,4-戊二烯酸，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇。本发明另外提供了使用这种微生物生产2,4-二戊二酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇的方法，通过培养含有2 4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径在条件和足够的时间内产生2,4-戊二烯酸，丁二烯，丙烯， 1,3-丁二醇，巴豆醇或3-丁烯-1-醇。

10. 发明申请

WO2012177599A3 MICROORGANISMS FOR PRODUCING N-PROPANOL 1, 3-PROPANEDIOL, 1,2-PROPANEDIOL OR GLYCEROL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产N-丙醇1,3-丙二醇，1,2-丙二醇或甘油的微生物及其相关方法
公开(公告)号：WO2012177599A3
公开(公告)日：2013-02-21
申请号：PCT/US2012043086
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/04 , C12N1/21
CPC分类号： C12P7/18 , C12N9/0008 , C12N9/0067 , C12P7/04 , C12P7/20
摘要： Provided herein is a non-naturally occurring microbial organism having a reductive TCA or Wood-Ljungdahl pathway and comprising at least one exogenous nucleic acid encoding said pathway enzymes expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. Also provided herein is a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, and can include culturing this organism for a sufficient period of time to produce a product.
摘要翻译：本文提供了具有还原性TCA或Wood-Ljungdahl途径的非天然存在的微生物生物体，并且包含至少一种编码所述途径酶的外源核酸，其以足够的量表达以增强通过乙酰辅酶A的碳通量。本文还提供了在一氧化碳或氢气存在下增强还原当量的可用性的方法，并且可以包括在足够的时间内培养该生物体以产生产物。

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