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1. 发明申请

WO2011140171A3 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF BUTADIENE 审中-公开
标题翻译：二苯乙烯生物合成的微生物和方法
公开(公告)号：WO2011140171A3
公开(公告)日：2012-04-26
申请号：PCT/US2011035105
申请日：2011-05-04
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P5/02 , C12N1/20
CPC分类号： C12P5/026 , C07C11/16 , C08F136/06 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
摘要翻译：本发明提供具有丁二烯途径的非天然存在的微生物。本发明另外提供了使用这种生物体来生产丁二烯的方法。

2. 发明申请

WO2012177619A3 MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产1,3-丁二醇的微生物及其相关方法
公开(公告)号：WO2012177619A3
公开(公告)日：2014-05-01
申请号：PCT/US2012043117
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P1/00 , C12N1/00 , C12N15/70 , C12N15/74
CPC分类号： C12P7/18 , C12N15/52
摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3 -BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3 -BDO pathway enzyme expressed in a sufficient amount to produce 1,3 -BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：本文提供了具有1,3-丁二醇（1,3-BOD）途径的非天然存在的微生物生物，并且包含至少一种编码1,3 -BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3 -BDO。在一些实施方案中，该途径包括从CO或氢中还原当量。在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

3. 发明申请

WO2012177601A3 MICROORGANISMS FOR PRODUCING ISOBUTANOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产ISOBUTANOL的微生物及其相关方法
公开(公告)号：WO2012177601A3
公开(公告)日：2014-05-08
申请号：PCT/US2012043091
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C07C29/00 , C12N1/00 , C12N1/20 , C12P7/16
CPC分类号： C12P7/16 , C12N15/52 , Y02E50/10
摘要： Provided herein is a non-naturally occurring microbial organism having an isopropanol pathway and comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. In certain embodiments, the pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. Also provided herein is anon-naturally occurring microbial organism having an n-butanol pathway and comprising at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism having n-butanol or isobutanol pathways are also provided herein. In certain embodiments, isobutanol pathways utilizing reverse TCA and/or reducing equivalents from CO and/or hydrogen are used to enhance product yields. The organisms provided herein can be cultured to produce isopropanol, n-butanol, or isobutanol.
摘要翻译：本文提供了具有异丙醇途径并且包含至少一种编码异丙醇途径酶的外源核酸的非天然存在的微生物生物。在某些实施方案中，该途径包括选自4-羟基丁酰-CoA脱水酶，巴豆酸酶，3-羟基丁酰辅酶A脱氢酶，乙酰乙酰辅酶A合成酶，乙酰辅酶A：乙酰乙酸酯-CoA转移酶，乙酰乙酰辅酶A 水解酶，乙酰乙酸脱羧酶和丙酮还原酶。本文还提供了具有正丁醇途径的非天然存在的微生物生物，并且包含至少一种编码正丁醇途径酶的外源核酸。本文还提供了其它具有正丁醇或异丁醇途径的非天然存在的微生物。在某些实施方案中，使用利用反向TCA和/或来自CO和/或氢的还原当量的异丁醇途径来提高产物产率。本文提供的生物体可以培养以产生异丙醇，正丁醇或异丁醇。

4. 发明申请

WO2012135789A3 MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO 审中-公开
标题翻译：生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法
公开(公告)号：WO2012135789A3
公开(公告)日：2014-05-01
申请号：PCT/US2012031733
申请日：2012-03-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00
CPC分类号： C12P7/40 , C12P7/42 , C12P7/62 , C12P19/32
摘要： The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
摘要翻译：本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

5. 发明申请

WO2012177599A3 MICROORGANISMS FOR PRODUCING N-PROPANOL 1, 3-PROPANEDIOL, 1,2-PROPANEDIOL OR GLYCEROL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产N-丙醇1,3-丙二醇，1,2-丙二醇或甘油的微生物及其相关方法
公开(公告)号：WO2012177599A3
公开(公告)日：2013-02-21
申请号：PCT/US2012043086
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/04 , C12N1/21
CPC分类号： C12P7/18 , C12N9/0008 , C12N9/0067 , C12P7/04 , C12P7/20
摘要： Provided herein is a non-naturally occurring microbial organism having a reductive TCA or Wood-Ljungdahl pathway and comprising at least one exogenous nucleic acid encoding said pathway enzymes expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. Also provided herein is a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, and can include culturing this organism for a sufficient period of time to produce a product.
摘要翻译：本文提供了具有还原性TCA或Wood-Ljungdahl途径的非天然存在的微生物生物体，并且包含至少一种编码所述途径酶的外源核酸，其以足够的量表达以增强通过乙酰辅酶A的碳通量。本文还提供了在一氧化碳或氢气存在下增强还原当量的可用性的方法，并且可以包括在足够的时间内培养该生物体以产生产物。

6. 发明申请

WO2012177983A4 MICROORGANISMS FOR PRODUCING ETHYLENE GLYCOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产乙二醇的微生物及其相关方法
公开(公告)号：WO2012177983A4
公开(公告)日：2013-06-20
申请号：PCT/US2012043714
申请日：2012-06-22
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms having an ethylene glycol pathway. The invention additionally provides methods of using such organisms to produce ethylene glycol. The invention provides non-naturally occurring microbial organisms containing ethylene glycol patways comprising at least one exogenous nucleic acid encoding an ethylene glycol pathway enzyme expressed in a sufficient amount to produce ethylene glycol. The invention additionally provides methods of using such microbial organisms to produce ethylene glycol, by culturing a non-naturally occurring microbial organism containing ethylene glycol pathways as described herein under conditions and for a sufficient period of time to produce ethylene glycol.
摘要翻译：本发明提供了具有乙二醇途径的非天然存在的微生物。本发明另外提供了使用这些生物体生产乙二醇的方法。本发明提供了包含至少一种编码乙二醇途径酶的外源核酸的含有乙二醇途径酶的非天然存在的微生物体，所述外源核酸以足以产生乙二醇的量表达。本发明另外提供了通过培养如本文所述的含有乙二醇途径的非天然存在的微生物有机体，在一定条件下培养足够的时间以产生乙二醇，从而使用这些微生物生产乙二醇的方法。

7. 发明申请

WO2012177983A3 MICROORGANISMS FOR PRODUCING ETHYLENE GLYCOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产乙烯甘油的微生物及其相关方法
公开(公告)号：WO2012177983A3
公开(公告)日：2013-05-16
申请号：PCT/US2012043714
申请日：2012-06-22
申请人： GENOMATICA INC , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms having an ethylene glycol pathway. The invention additionally provides methods of using such organisms to produce ethylene glycol. The invention provides non-naturally occurring microbial organisms containing ethylene glycol patways comprising at least one exogenous nucleic acid encoding an ethylene glycol pathway enzyme expressed in a sufficient amount to produce ethylene glycol. The invention additionally provides methods of using such microbial organisms to produce ethylene glycol, by culturing a non-naturally occurring microbial organism containing ethylene glycol pathways as described herein under conditions and for a sufficient period of time to produce ethylene glycol.
摘要翻译：本发明提供了具有乙二醇途径的非天然存在的微生物。本发明另外提供了使用这些生物体来生产乙二醇的方法。本发明提供含有乙二醇培养物的非天然存在的微生物，所述微生物包含至少一种编码乙二醇途径酶的外源核酸，所述外源核酸以足够的量表达以产生乙二醇。本发明还提供了使用这种微生物生产乙二醇的方法，通过在条件和足够的时间内培养含有本文所述的乙二醇途径的非天然存在的微生物生物来生产乙二醇。

8. 发明申请

WO2009135074A2 MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 审中-公开
标题翻译：用于生产甲基丙烯酸的微生物
公开(公告)号：WO2009135074A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042426
申请日：2009-04-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00 , C12P7/40
CPC分类号： C12P7/42 , C12N9/0008 , C12N9/1096 , C12N9/88 , C12N9/90 , C12P7/40 , C12Y102/01 , C12Y206/01022 , C12Y403/01 , C12Y504/99002
摘要： The invention provides a non-naturally occurring microbial organism having a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3- hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
摘要翻译：本发明提供了具有2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径的非天然存在的微生物生物。微生物生物体含有编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径中的酶的至少一种外源核酸。本发明还提供了2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸的制备方法。该方法可以包括培养足够量的表达至少一种编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径酶的外源核酸的2-羟基异丁酸，3-羟基异丁酸或产生甲基丙烯酸的微生物，并培养在条件下和足够的时间内产生2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸。

9. 发明申请

WO2012018624A2 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE 审中-公开
标题翻译：微生物和芳香化合物，2,4-十五碳烯酸酯和1,3-丁二烯的生物合成方法
公开(公告)号：WO2012018624A2
公开(公告)日：2012-02-09
申请号：PCT/US2011045364
申请日：2011-07-26
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , BURGARD ANTHONY P , PHARKYA PRITI , BURK MARK J
发明人： OSTERHOUT ROBIN E , BURGARD ANTHONY P , PHARKYA PRITI , BURK MARK J
IPC分类号： C12P5/00 , C12P5/02 , C12P7/40
CPC分类号： C12P7/62 , C12N15/52 , C12P5/005 , C12P5/026 , C12P7/16 , C12P7/40 , C12P9/00 , Y02E50/10
摘要： The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-lol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4- oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4- pentadienoate, 3-butene-lol or 1,3-butadiene.
摘要翻译：本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯的非天然存在的微生物，2,4-戊二烯酸酯，3-丁烯醇或1,3-丁二烯途径。本发明还提供了使用这些生物体生产甲苯，苯，对甲苯甲酸，对苯二甲酸，（2-羟基-3-甲基-4-氧代丁氧基）膦酸，（2-羟基-4-氧代丁氧基）膦酸，苯甲酸，苯乙烯，2,4-戊二烯酸酯，3-丁烯醇或1,3-丁二烯。

10. 发明申请

WO2010127319A3 ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开
标题翻译：生产1,3-丁二醇的有机体
公开(公告)号：WO2010127319A3
公开(公告)日：2010-12-23
申请号：PCT/US2010033300
申请日：2010-04-30
申请人： GENOMATICA INC , BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P7/16 , C12N1/20
CPC分类号： C12P7/18 , C07C29/80 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/01 , C12Y101/01157 , C12Y102/01 , C12Y203/01 , C12Y206/01 , C12Y401/01 , C12Y402/01 , C12Y403/01
摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：非天然存在的微生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。该途径包括选自2-氨基-4-酮戊酸（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基丁基乙酰辅酶A还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

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