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1. 发明申请

WO2010141920A3 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物及相关方法
公开(公告)号：WO2010141920A3
公开(公告)日：2011-01-27
申请号：PCT/US2010037544
申请日：2010-06-04
申请人： GENOMATICA INC , VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
发明人： VAN DIEN STEPHEN J , BURGARD ANTHONY P , HASELBECK ROBERT , PUJOL-BAXLEY CATHERINE J , NIU WEI , TRAWICK JOHN D , YIM HARRY , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN
IPC分类号： C12P7/16
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化BDO的表达。本发明还提供了使用这种微生物生产BDO的方法。

2. 发明申请

WO2011140171A3 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF BUTADIENE 审中-公开
标题翻译：二苯乙烯生物合成的微生物和方法
公开(公告)号：WO2011140171A3
公开(公告)日：2012-04-26
申请号：PCT/US2011035105
申请日：2011-05-04
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , SUN JUN , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P5/02 , C12N1/20
CPC分类号： C12P5/026 , C07C11/16 , C08F136/06 , C12N15/52
摘要： The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
摘要翻译：本发明提供具有丁二烯途径的非天然存在的微生物。本发明另外提供了使用这种生物体来生产丁二烯的方法。

3. 发明申请

WO2010030711A3 MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL 审中-公开
标题翻译：用于生产1,4-丁二醇的微生物
公开(公告)号：WO2010030711A3
公开(公告)日：2010-10-07
申请号：PCT/US2009056415
申请日：2009-09-09
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN
IPC分类号： C12P7/16 , C12P1/04
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/1096 , C12N9/12 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/70 , C12N15/81 , C12Y101/01157 , C12Y402/01055
摘要： The invention provides non- naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
摘要翻译：本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量产生BDO的BDO途径酶的外源核酸。本发明还提供了使用这种微生物生产BDO的方法。

4. 发明申请

WO2012177619A3 MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产1,3-丁二醇的微生物及其相关方法
公开(公告)号：WO2012177619A3
公开(公告)日：2014-05-01
申请号：PCT/US2012043117
申请日：2012-06-19
申请人： GENOMATICA INC , BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P1/00 , C12N1/00 , C12N15/70 , C12N15/74
CPC分类号： C12P7/18 , C12N15/52
摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3 -BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3 -BDO pathway enzyme expressed in a sufficient amount to produce 1,3 -BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：本文提供了具有1,3-丁二醇（1,3-BOD）途径的非天然存在的微生物生物，并且包含至少一种编码1,3 -BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3 -BDO。在一些实施方案中，该途径包括从CO或氢中还原当量。在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

5. 发明申请

WO2012135789A3 MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO 审中-公开
标题翻译：生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法
公开(公告)号：WO2012135789A3
公开(公告)日：2014-05-01
申请号：PCT/US2012031733
申请日：2012-03-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , SUN JUN , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00
CPC分类号： C12P7/40 , C12P7/42 , C12P7/62 , C12P19/32
摘要： The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
摘要翻译：本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

6. 发明申请

WO2009135074A2 MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 审中-公开
标题翻译：用于生产甲基丙烯酸的微生物
公开(公告)号：WO2009135074A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042426
申请日：2009-04-30
申请人： GENOMATICA INC , BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURK MARK J , BURGARD ANTHONY P , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P7/52 , C12N1/00 , C12P7/40
CPC分类号： C12P7/42 , C12N9/0008 , C12N9/1096 , C12N9/88 , C12N9/90 , C12P7/40 , C12Y102/01 , C12Y206/01022 , C12Y403/01 , C12Y504/99002
摘要： The invention provides a non-naturally occurring microbial organism having a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3- hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
摘要翻译：本发明提供了具有2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径的非天然存在的微生物生物。微生物生物体含有编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径中的酶的至少一种外源核酸。本发明还提供了2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸的制备方法。该方法可以包括培养足够量的表达至少一种编码2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸途径酶的外源核酸的2-羟基异丁酸，3-羟基异丁酸或产生甲基丙烯酸的微生物，并培养在条件下和足够的时间内产生2-羟基异丁酸，3-羟基异丁酸或甲基丙烯酸。

7. 发明申请

WO2012018624A2 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE 审中-公开
标题翻译：微生物和芳香化合物，2,4-十五碳烯酸酯和1,3-丁二烯的生物合成方法
公开(公告)号：WO2012018624A2
公开(公告)日：2012-02-09
申请号：PCT/US2011045364
申请日：2011-07-26
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , BURGARD ANTHONY P , PHARKYA PRITI , BURK MARK J
发明人： OSTERHOUT ROBIN E , BURGARD ANTHONY P , PHARKYA PRITI , BURK MARK J
IPC分类号： C12P5/00 , C12P5/02 , C12P7/40
CPC分类号： C12P7/62 , C12N15/52 , C12P5/005 , C12P5/026 , C12P7/16 , C12P7/40 , C12P9/00 , Y02E50/10
摘要： The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-lol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4- oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4- pentadienoate, 3-butene-lol or 1,3-butadiene.
摘要翻译：本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯的非天然存在的微生物，2,4-戊二烯酸酯，3-丁烯醇或1,3-丁二烯途径。本发明还提供了使用这些生物体生产甲苯，苯，对甲苯甲酸，对苯二甲酸，（2-羟基-3-甲基-4-氧代丁氧基）膦酸，（2-羟基-4-氧代丁氧基）膦酸，苯甲酸，苯乙烯，2,4-戊二烯酸酯，3-丁烯醇或1,3-丁二烯。

8. 发明申请

WO2010127319A3 ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开
标题翻译：生产1,3-丁二醇的有机体
公开(公告)号：WO2010127319A3
公开(公告)日：2010-12-23
申请号：PCT/US2010033300
申请日：2010-04-30
申请人： GENOMATICA INC , BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , PHARKYA PRITI
发明人： BURGARD ANTHONY P , BURK MARK J , OSTERHOUT ROBIN E , PHARKYA PRITI
IPC分类号： C12P7/16 , C12N1/20
CPC分类号： C12P7/18 , C07C29/80 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/01 , C12Y101/01157 , C12Y102/01 , C12Y203/01 , C12Y206/01 , C12Y401/01 , C12Y402/01 , C12Y403/01
摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：非天然存在的微生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。该途径包括选自2-氨基-4-酮戊酸（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基丁基乙酰辅酶A还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

9. 发明申请

WO2013028519A4 MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS 审中-公开
标题翻译：用于生产2,4-二戊二酸，二丁烯，丙烯，1,3-丁二醇和相关醇的微生物和方法
公开(公告)号：WO2013028519A4
公开(公告)日：2013-05-10
申请号：PCT/US2012051347
申请日：2012-08-17
申请人： GENOMATICA INC , OSTERHOUT ROBIN E , BURGARD ANTHONY P , BURK MARK J
发明人： OSTERHOUT ROBIN E , BURGARD ANTHONY P , BURK MARK J
IPC分类号： C12P7/18
CPC分类号： C12P7/16 , C12N15/70 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/40 , Y02E50/10
摘要： The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non- naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.
摘要翻译：本发明提供非天然存在的微生物，其含有2,4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径，其包含至少一种编码丁二烯途径酶的外源核酸，足量生产2,4-戊二烯酸，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇。本发明另外提供了使用这种微生物生产2,4-二戊二酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇的方法，通过培养含有2 4-戊二烯酸酯，丁二烯，丙烯，1,3-丁二醇，巴豆醇或3-丁烯-1-醇途径在条件和足够的时间内产生2,4-戊二烯酸，丁二烯，丙烯， 1,3-丁二醇，巴豆醇或3-丁烯-1-醇。

10. 发明申请

WO2008115840A3 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
标题翻译： 1,4-丁二醇及其前体的生物合成的组合物和方法
公开(公告)号：WO2008115840A3
公开(公告)日：2009-01-29
申请号：PCT/US2008057168
申请日：2008-03-14
申请人： GENOMATICA INC , BURK MARK J , VAN DIEN STEPHEN J , BURGARD ANTHONY , NIU WEI
发明人： BURK MARK J , VAN DIEN STEPHEN J , BURGARD ANTHONY , NIU WEI
IPC分类号： C12P1/00
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or a-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, a-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4- butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a A- hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or a-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric A- hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, a-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.
摘要翻译：本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为以足够的量表示产生1,4-丁二醇（BDO）。另外提供了生产4-HB的方法。该方法包括培养具有4-羟基丁酸（4-HB）生物合成途径的非天然存在的微生物生物，其包含至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，辅酶A依赖性琥珀酸半醛脱氢酶或 - 酮戊二酸脱羧酶在充分的厌氧条件下持续足够的时间以产生单体的4-羟基丁酸（4-HB）。还提供了用于生产BDO的方法。该方法包括培养非天然存在的微生物生物催化剂，其包含具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4- 羟基丁酸脱氢酶，琥珀酰-CoA合成酶，CoA依赖性琥珀酸脱氢酶，4-羟基丁酸酯：CoA转移酶，4-羟基丁酸酯激酶，磷酸转移羟基丁酸酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，时间来生产1,4-丁二醇（BDO）。 4-HB和/或BDO产物可以分泌到培养基中。

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