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    • 4. 发明申请
    • NEUROTOXINS EXHIBITING SHORTENED BIOLOGICAL ACTIVITY
    • 神经毒素展示生物活性
    • WO2013068476A1
    • 2013-05-16
    • PCT/EP2012/072158
    • 2012-11-08
    • MERZ PHARMA GMBH & CO. KGAASCHMIDT, MichaelFREVERT, JürgenHOFMANN, FredGROER, Gerhard
    • SCHMIDT, MichaelFREVERT, JürgenHOFMANN, FredGROER, Gerhard
    • C07K14/47C07K14/435C07K14/33C12N5/00A61K38/00
    • C12N9/52A61K38/4893C07K14/4746C12Y304/24069
    • The present invention relates to the pharmaceutical field. Specifically, it contemplates a polynucleotide encoding a neurotoxin polypeptide exhibiting a reduced duration of the biological effect in a subject, wherein said polypeptide comprises at least one E3 ligase recognition motif in the light chain, wherein said E3 ligase recognition motif is preferably abinding motif for the E3 ligase MDM2. The invention further pertains to polypeptides encoded by the polynucleotide of the invention as well as polypeptides comprising one or more amino acid substitutions. Further encompassed by the present invention are vectors and host cells comprising the said polynucleotide, polypeptides encoded thereby and antibodies specifically binding to the polypeptides. Moreover, the invention relates to medicaments comprising said polynucleotides and polypeptides, as well as specific therapeutic applications thereof. Furthermore, the present invention contemplates methods for the manufacture of the polypeptides and medicaments.
    • 本发明涉及制药领域。 具体地,其考虑了编码神经毒素多肽的多核苷酸,其在受试者中显示生物效应的持续时间减少,其中所述多肽在轻链中包含至少一个E3连接酶识别基序,其中所述E3连接酶识别基序优选为 E3连接酶MDM2。 本发明还涉及由本发明的多核苷酸编码的多肽以及包含一个或多个氨基酸取代的多肽。 本发明还包括载体和宿主细胞,其包含所述多核苷酸,由其编码的多肽和与多肽特异性结合的抗体。 此外,本发明涉及包含所述多核苷酸和多肽的药物,以及其具体的治疗应用。 此外,本发明考虑了制备多肽和药物的方法。
    • 10. 发明申请
    • PROCESS FOR PRODUCTION OF A FASTENER AND FASTENER FOR AN ELONGATED OBJECT
    • 生产紧固件的紧固件和紧固件的方法
    • WO2009130146A1
    • 2009-10-29
    • PCT/EP2009/054437
    • 2009-04-15
    • NEWFREY LLCKUMPF, MarkusSCHMIDT, MichaelROSEMANN, Frank
    • KUMPF, MarkusSCHMIDT, MichaelROSEMANN, Frank
    • B29C45/16
    • B29C45/1645B29L2031/24F16L3/221F16L3/223F16L3/237F16L55/035
    • For molding a fastener (10) for an elongated object, where the fastener (10) has a section which forms an object holder (12), and a fastening section (11), a mono- sandwich injection molding process is used, where a given quantity of a first synthetic material component and a second synthetic material component are fed into a melting and injection cylinder and then are continuously pressed, one after the other, by the cylinder into a mold cavity forming the fastener (10) in a stratification such that the first synthetic material component reaches the mold cavity before the second synthetic material component and forms a surface part (O) in the region of the object holder (12) and the second synthetic material component forms a core part (K) connected substance-lockingly with the surface part (O), which comprises the object holder (12) and the fastening section (11).
    • 为了模制用于细长物体的紧固件(10),其中紧固件(10)具有形成物体保持器(12)的部分和紧固部分(11),使用单夹层注射成型工艺,其中 将给定量的第一合成材料组分和第二合成材料组分进料到熔化和注射圆筒中,然后通过圆筒一个接一个地连续地压入形成紧固件(10)的模腔中,分层如下 所述第一合成材料成分在所述第二合成材料成分之前到达所述模腔,并且在所述物体保持件(12)的区域中形成表面部分(O),并且所述第二合成材料成分形成连接物质的核心部分(K) 与包括物体保持器(12)和紧固部分(11)的表面部分(O)锁定。