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1. 发明申请

WO1997045202A1 SUPERPARAMAGNETIC MONODISPERSED PARTICLES 审中-公开
标题翻译：超级粒子单体颗粒
公开(公告)号：WO1997045202A1
公开(公告)日：1997-12-04
申请号：PCT/FR1997000912
申请日：1997-05-23
申请人： BIO MERIEUX , ELAISSARI, Abdelhamid , PICHOT, Christian , MANDRAND, Bernard , SAUZEDDE, Florence
发明人： BIO MERIEUX
IPC分类号： B03C01/01
CPC分类号： H01F1/37 , B03C1/01 , G01N33/5375 , G01N33/5434 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , H01F1/44 , Y10S436/806 , Y10S436/824 , Y10T428/2982 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998 , Y10T436/24
摘要： The invention discloses superparamagnetic monodispersed particles, their method of preparation and their uses. These particles comprise: a core of a first polymer, an internal layer of a second polymer coating the core, and in which is distributed a magnetic material, and an external layer of a third polymer coating the magnetic layer, and capable of interacting with at least one biological molecule, at least the second polymer being heat sensitive and having a predetermined lower critical solubility temperature (LCST) of 15 to 65 DEG C.
摘要翻译：本发明公开了超顺磁单分散颗粒，其制备方法及其用途。这些颗粒包括：第一聚合物的芯，涂覆芯的第二聚合物的内层，其中分布有磁性材料，以及涂覆磁性层的第三聚合物的外层，并且能够与至少一个生物分子，至少第二聚合物是热敏感的并具有15至65℃的预定的较低临界溶解温度（LCST）。

2. 发明申请

WO1997034909A1 NUCLEIC ACID ISOLATION 审中-公开
标题翻译：核酸分离
公开(公告)号：WO1997034909A1
公开(公告)日：1997-09-25
申请号：PCT/FR1997000496
申请日：1997-03-20
申请人： BIO MERIEUX , CROS, Philippe , ELAISSARI, Abdelhamid , MABILAT, Claude , PICHOT, Christian , RODRIGUE, Marc , SANTORO, Lise
发明人： BIO MERIEUX
IPC分类号： C07H01/08
CPC分类号： C12N15/101 , C12N15/1006 , C12Q1/6806
摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) providing an adsorption reagent that includes a sol consisting of an aqueous continuous phase and a dispersed particulate substrate phase including a functionalised particulate polymer prepared by polymerising (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45 DEG C; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10 M is achieved.
摘要翻译：公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。该方法包括一种吸附试剂制备步骤（a），该步骤（a）提供一种吸附试剂，该吸附试剂包括由含水连续相组成的溶胶和分散的颗粒基质相，所述分散的颗粒底物相包括通过聚合制备的官能化颗粒聚合物（1）第一水溶性丙烯酰胺或丙烯酰胺衍生单体，（2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解温度（LCST）; 接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。

3. 发明申请

WO1997035031A1 NUCLEOTIDE SEQUENCE DETECTION WITH SIGNAL AMPLIFICATION 审中-公开
标题翻译：核苷酸序列检测与信号放大
公开(公告)号：WO1997035031A1
公开(公告)日：1997-09-25
申请号：PCT/FR1997000483
申请日：1997-03-19
申请人： BIO MERIEUX , DELAIR, Thierry , ELAISSARI, Abdelhamid , CHARLES, Marie-Hélène , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6834 , C12Q1/682
摘要： A kit for detecting a nucleotide sequence of interest with signal amplification, containing a labelled nucleotide probe and a reagent essentially including a suspension of particles on which at least one series of oligonucleotide units is immobilised. Each of the series of oligonucleotide units, which are all identical, includes, at least, a nucleotide sequence hybridizable with said sequence of interest and a nucleotide sequence hybridizable with said probe. Said reagent contains more than 10 such oligonucleotide units per particle. The kit is particularly useful for diagnosing genetic diseases or determining pathogens such as bacteria, viruses, fungi or parasites.

4. 发明申请

WO1995008000A2 REAGENT AND METHOD FOR THE DETECTION OF A NUCLEOTIDE SEQUENCE WITH SIGNAL AMPLIFICATION 审中-公开
标题翻译：用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：WO1995008000A2
公开(公告)日：1995-03-23
申请号：PCT/FR1994001084
申请日：1994-09-15
申请人： BIO MERIEUX , MANDRAND, Bernard , CROS, Philippe , DELAIR, Thierry , CHARLES, Marie-Hélène , EROUT, Marie-Noëlle , PICHOT, Christian
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Kit for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The kit contains, in suitable containers, a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with said probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefore lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译：用于检测感兴趣的核苷酸序列的试剂盒，其包含用示踪剂标记的至少一个核苷酸探针。试剂盒在合适的容器中含有基本上包含具有侧向取代基的线性骨架共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中至少一部分单元第一类型具有包含核苷酸单元的侧向取代基，这种侧向取代基不存在于其它类型的单元上。所述核苷酸单元中的每一个都相同，包含至少一个能够与所述感兴趣的序列杂交的核苷酸序列和能够与所述探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元，摩尔当量，每摩尔聚合物。这样的试剂能够获得信号放大，因此降低灵敏度阈值。特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

5. 发明申请

WO1997004126A1 METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES BY TRANSLOCATION USING CHIMERIC PRIMERS 审中-公开
标题翻译：通过使用CHIMERIC PRIMERS进行转录来放大核酸序列的方法
公开(公告)号：WO1997004126A1
公开(公告)日：1997-02-06
申请号：PCT/FR1996001166
申请日：1996-07-24
申请人： BIO MERIEUX , CLEUZIAT, Philippe , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6844 , C12Q2537/113 , C12Q2527/101 , C12Q2521/313 , C12Q2531/119 , C12Q2525/121
摘要： A target nucleic acid sequence (12) may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer (A1, A2) that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridising with a 3'-terminal segment of the target and a DNA-type segment capable of hybridising with a segment adjacent to said 3'-terminal segment of the target, and a DNA- or RNA-type primer B1 capable of hybridising with said 3'-terminal segment of the target. A cyclic amplification is achieved that may be implemented isothermally on the basis of either a DNA or an RNA target even when the terminals are not defined.
摘要翻译：可以通过使用包含5'至3'方向的嵌合引物（A1，A2）在包含DNA聚合酶，链转位和RNAse H活性的酶系统存在下扩增靶核酸序列（12）能够与靶标的3'末端区段杂交的RNA型区段和能够与靶标的3'-末端区段相邻的区段杂交的DNA型区段，以及DNA型或RNA型区段能够与目标的所述3'-末端区段杂交的引物B1。实现了循环扩增，其可以基于DNA或RNA靶标等温地实现，即使在末端未定义时也是如此。

6. 发明申请

WO1995021859A1 CYTOTOXIC FACTOR ASSOCIATED WITH DISSEMINATED SCLEROSIS, ITS DETECTION AND QUANTIFICATION 审中-公开
标题翻译：与感染性SCLEROSIS相关的细胞因子，其检测和定量
公开(公告)号：WO1995021859A1
公开(公告)日：1995-08-17
申请号：PCT/FR1995000178
申请日：1995-02-15
申请人： BIO MERIEUX , PERRON, Hervé , DOBRANSKY, Tomas , RIEGER, François , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C07K14/47
CPC分类号： C07K14/4713 , A61K38/00 , G01N33/5091 , Y10S424/16 , Y10S435/964 , Y10S436/827 , Y10S436/828
摘要： Gliotoxic factor, in isolated or purified condition, characterized in that it has a toxic activity on animal or human astrocytic cells resulting in a cytomorphological disorganization of their network of intermediary filaments and/or a degradation of the proteins of said intermediary filaments and/or cell death particularly, by apoptosis.
摘要翻译：在分离或纯化条件下的胶质毒性因子，其特征在于其对动物或人星形细胞细胞具有毒性活性，导致其中间丝的网络的细胞形态学紊乱和/或所述中间丝和/或细胞的蛋白质的降解死亡特别是凋亡。

7. 发明申请

WO1993002213A1 PROBE SYSTEM FOR PERFORMING HLA DR TYPING AND TYPING METHOD USING SAID PROBES 审中-公开
标题翻译：用于执行HLA DR类型的探测系统和使用所述探测器的类型方法
公开(公告)号：WO1993002213A1
公开(公告)日：1993-02-04
申请号：PCT/FR1992000702
申请日：1992-07-17
申请人： BIO MERIEUX , ALLIBERT, Patrice, André , GROS, Philippe , MACH, Bernard, François , MANDRAND, Bernard, Fabien , TIERCY, Jean-Marie
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6881 , C12Q2600/156
摘要： A set of nucleotide probes for determining HLA DR types and/or related types by means of commonly used typing techniques using oligonucleotides, wherein said set of probes includes at least the following nucleotide probes (the underligned portion represents the optimal sequence) (I). Said probes may be labelled, covalently coupled to a ligand which facilitates their attachment to a solid support, or attached to a solid support. The use of the probes in a HLA DR typing method, e.g. as capture probes according to the sandwich technique, is also described.

8. 发明申请

WO1998011439A1 METHOD FOR DETECTING AND/OR QUANTIFYING A GLIOTOXIC FACTOR 审中-公开
标题翻译：检测和/或定量GLIOTOXIC因子的方法
公开(公告)号：WO1998011439A1
公开(公告)日：1998-03-19
申请号：PCT/FR1997001620
申请日：1997-09-12
申请人： BIO MERIEUX , MALCUS-VOCANSON, Carine , PERRON, Hervé , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： G01N33/564
CPC分类号： G01N33/564 , G01N33/5014 , G01N33/6896
摘要： The invention proposes a method for detecting and/or quantifying, in a biological sample a cytotoxic factor, in particular a gliotoxic factor, with respect to adherent target cells, in particular macroglial cells, the toxicity of which causes the death by apoptosis of said cells. The method consists in providing an initial fraction of said sample, optionally enriched with said toxic factor by previous treatment, incubating said initial toxic factor with a reference culture medium comprising adherent target cells, and detecting and/or quantifying the adherent target cells killed by apoptosis, by flux cytometry, at least one direct or indirect characteristic associated with the apoptotic adherent cells of the whole or part of the incubated medium, which, if it is present and/or is quantified, qualifies the sample as positive, i.e. as containing said toxic factor. The initial biological sample is preferably a urine specimen.
摘要翻译：本发明提出了一种用于在生物样品中检测和/或定量细胞毒性因子，特别是胶质毒性因子的方法，涉及粘附靶细胞，特别是大胶质细胞，其毒性通过所述细胞的凋亡导致死亡。该方法包括提供所述样品的初始级分，任选地通过先前的处理富集所述毒性因子，将所述初始毒性因子与包含贴壁靶细胞的参照培养基孵育，并检测和/或定量由细胞凋亡杀死的贴壁靶细胞通过流式细胞术，与整个或部分培养的培养基的凋亡贴壁细胞相关的至少一种直接或间接特征，如果存在和/或定量，则将样品鉴定为阳性，即含有所述毒性因素。初始生物样品优选为尿样。

9. 发明申请

WO1995021256A1 MSRV1 VIRUS AND MSRV2 PATHOGENIC AND/OR INFECTIOUS AGENT ASSOCIATED WITH MULTIPLE SCLEROSIS, NUCLEIC ACID COMPONENTS AND APPLICATIONS OF SAME 审中-公开
标题翻译： MSRV1病毒和MSRV2致病和/或感染代谢相关的多发性糖尿病，核酸成分及其应用
公开(公告)号：WO1995021256A1
公开(公告)日：1995-08-10
申请号：PCT/FR1995000142
申请日：1995-02-06
申请人： BIO MERIEUX , PERRON, Hervé , MALLET, François , MANDRAND, Bernard , BEDIN, Frédéric , BESEME, Frédéric
发明人： BIO MERIEUX
IPC分类号： C12N15/48
CPC分类号： C07K14/005 , A61K38/00 , C12N7/00 , C12N2740/10021 , C12N2740/10022 , Y10S435/912
摘要： Composition comprising two pathogenic and/or infectious agents associated with multiple sclerosis, namely, a first agent comprising a human virus with reverse transcriptase activity, and related to a family of endogenous retroviral elements, or a variant thereof, and a second agent, or a variant thereof, wherein the two said pathogenic and/or infectious agents originate from the same viral strain selected from the strains respectively designated as POL-2, registered on 22.07.1992 with ECACC under accession number V92072202, and MS7PG, registered on 08.01.93 with ECACC under accession number V93010816, or from their variant strains.
摘要翻译：包含与多发性硬化症相关的两种致病和/或感染因子的组合物，即包含具有逆转录酶活性的人病毒并与内源性逆转录病毒元件家族或其变体相关的第一药剂和第二药剂，或其中所述两种病原体和/或感染因子源自选自2003年22.07.1992号登记号为V92072202的ECACC分别指定为POL-2的菌株和08.01.93登记的MS7PG的相同病毒株 ECACC保藏号为V93010816，或其变体菌株。

10. 发明申请

WO1991019812A1 METHOD FOR DETECTING A NUCLEOTIDE SEQUENCE BY SANDWICH HYBRIDIZATION 审中-公开
标题翻译：通过三聚体杂化检测核苷酸序列的方法
公开(公告)号：WO1991019812A1
公开(公告)日：1991-12-26
申请号：PCT/FR1991000468
申请日：1991-06-11
申请人： BIO MERIEUX , CROS, Philippe , ALLIBERT, Patrice , MALLET, François , MABILAT, Claude , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6883 , C12Q1/6813 , C12Q1/6834 , C12Q1/6886 , C12Q1/689 , C12Q1/703 , C12Q1/708
摘要： A method for detecting a single-stranded nucleotide sequence in a sample which is known or thought to contain it, using sandwich hybridization comprising the step of incubating the sample with a capture probe which is passively fixed to a solid support and with a detector probe which is labelled with a non-radioactive marker. The capture and detector probes can hybridize with two non-overlapping regions respectively of the target nucleotide sequence being sought. The incubation step is followed by a washing step for removing reagents which have not been fixed by hybridization. The method is characterized in that the capture probe contains 9-30 nucleotides and is fixed to said solid support which is made of a material based on a hydrophobic compound. The method can be used particularly for diagnosing infectious or genetic diseases and for cell typing.

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