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1. 发明申请

WO2019067973A1 ENRICHMENT OF SHORT NUCLEIC ACID FRAGMENTS IN SEQUENCING LIBRARY PREPARATION 审中-公开
公开(公告)号：WO2019067973A1
公开(公告)日：2019-04-04
申请号：PCT/US2018/053536
申请日：2018-09-28
申请人： GRAIL, INC.
发明人： JUNG, Byoungsok , ARAVANIS, Alex
IPC分类号： C12Q1/6869 , C12N15/10
CPC分类号： C12N15/1058 , C12N15/101 , C12N15/1093 , C12N15/1096 , C12Q1/6844 , C12Q1/6869 , C12Q1/6874 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/16 , C40B20/00 , C40B40/08 , C12Q2523/305 , C12Q2525/186 , C12Q2525/191 , C12Q2525/204
摘要： Methods for preparing enriched sequencing libraries from test samples that contain double-stranded deoxyribonucleic acid (dsDNA) are provided.

2. 发明申请

WO2016193282A1 ELECTROPHORESIS ASSISTED METHOD FOR PURIFYING A TARGET NUCLEIC ACID USING A DELAYED ELUTION APPROACH 审中-公开
标题翻译：电泳辅助方法，用于使用延迟的方法来净化目标核酸
公开(公告)号：WO2016193282A1
公开(公告)日：2016-12-08
申请号：PCT/EP2016/062329
申请日：2016-06-01
申请人： QIAGEN GMBH
发明人： SINGER, Thorsten , FAKIH, Sarah
IPC分类号： C12N15/09
CPC分类号： C12N15/101 , B01D15/203 , B01D15/426 , B01D57/02 , C12N15/1013 , G01N27/44704
摘要： Provided is inter alia to an electrophoresis assisted method for purifying a target nucleic acid from a nucleic acid containing sample, comprising: (a) binding the target nucleic acid to a solid phase; (b) placing the solid phase with the bound target nucleic acid into a loading chamber of a device, wherein the device comprises a passage which comprises the loading chamber, optionally a liquid permeable separation matrix adjacent to the loading chamber, and a liquid permeable collection matrix and wherein the solid phase with the bound target nucleic acid is present in the loading chamber in a liquid medium comprising at least one water-miscible organic solvent and wherein the target nucleic acid remains bound to the solid phase in said liquid medium; (c) generating an electric field between a cathode and an anode and using a running solution that conducts the electric current, wherein the running solution dilutes the liquid medium comprised in the loading chamber resulting in elution of the bound target nucleic acid, and wherein the eluted target nucleic acid migrates according to its charge in the electric field and is retained by the collection matrix; (d) collecting the purified target nucleic acid. The method is particularly suitable for isolating RNA. The liquid medium delays elution of the RNA from the solid phase, thereby preventing a degradation of the RNA by e.g. RNases.
摘要翻译：本发明尤其涉及从含有核酸的样品中纯化靶核酸的电泳辅助方法，其包括：（a）将靶核酸与固相结合; （b）将固相与结合的靶核酸置于装置的装载室中，其中所述装置包括通道，该通道包括装载室，任选地与装载室相邻的液体可渗透分离基质，以及液体可渗透收集物并且其中具有结合的靶核酸的固相在装载室中存在于包含至少一种与水可混溶的有机溶剂的液体介质中，并且其中所述靶核酸保持与所述液体介质中的固相结合; （c）在阴极和阳极之间产生电场并使用传导电流的运行溶液，其中运行的溶液稀释装载室中包含的液体介质，导致结合的靶核酸的洗脱，并且其中洗脱的靶核酸根据其在电场中的电荷迁移并被收集矩阵保留; （d）收集纯化的靶核酸。该方法特别适用于分离RNA。液体介质延迟了RNA从固相的洗脱，从而防止了RNA的降解。核糖核酸。

3. 发明申请

WO2016083793A1 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2016083793A1
公开(公告)日：2016-06-02
申请号：PCT/GB2015/053573
申请日：2015-11-24
申请人： GENEVILLAGE KFT , HART, Deborah Mary
发明人： JENEY, Csaba
IPC分类号： C12N15/10 , G01N33/68
CPC分类号： G01N33/6845 , C12N15/10 , C12N15/101 , C12N15/1037 , C12Q1/6834 , C12Q1/6853 , C12Q1/686 , C12Q1/6872 , G01N30/02 , G01N33/68 , G01N2458/10 , G01N2500/04
摘要： The present invention relates generally methods and kits for detecting binding interactions, in particular protein-protein interactions, and particularly to high throughput methods for labelling, analysing, detecting and measuring protein-protein interactions.
摘要翻译：本发明一般涉及用于检测结合相互作用，特别是蛋白质 - 蛋白质相互作用的方法和试剂盒，特别涉及用于标记，分析，检测和测量蛋白质 - 蛋白质相互作用的高通量方法。

4. 发明申请

WO2015106146A3 SIMULTANEOUS EXTRACTION AND SEPARATION OF RNA AND DNA FROM SINGLE CELLS USING ELECTROPHORETIC TECHNIQUES 审中-公开
标题翻译：使用电泳技术从单细胞中同时提取和分离RNA和DNA
公开(公告)号：WO2015106146A3
公开(公告)日：2016-04-07
申请号：PCT/US2015010884
申请日：2015-01-09
申请人： UNIV LELAND STANFORD JUNIOR
发明人： SANTIAGO JUAN G , SHINTAKU HIROFUMI
IPC分类号： B01D57/02
CPC分类号： A61B1/00009 , A61B1/0005 , A61B1/05 , A61B1/0676 , C12N15/101 , C12Q1/6806 , G01N27/44791 , G06T7/0016 , G06T7/254 , G06T2207/10068 , G06T2207/30032 , C12Q2565/125 , C12Q2565/629
摘要： Devices and methods for preparing RNA and DNA from single cells are disclosed. In particular, the invention relates to a method of simultaneously extracting RNA and DNA from single cells and separating the nucleic acids electrophoretically. An electric field is used to lyse a single target cell, such that the plasma membrane is selectively disrupted without lysing the nuclear membrane. Cytoplasmic RNA is separated from the nucleus by performing isotachophoresis (ITP) in the presence of a sieving matrix that preferentially reduces the mobility of the nucleus. During ITP, the cytoplasmic RNA accumulates at an ITP interface between leading and trailing electrolytes and can later be extracted free of nuclear DNA. The method can be performed in a microfluidic device that fully automates all steps of the process.
摘要翻译：公开了从单细胞制备RNA和DNA的装置和方法。特别地，本发明涉及从单个细胞同时提取RNA和DNA并且电泳分离核酸的方法。电场用于裂解单个靶细胞，使得质膜选择性地破裂而不裂解核膜。在筛选基质的存在下，通过进行等速电泳（ITP），从细胞质中分离细胞质RNA，其优先降低细胞核的迁移率。在ITP期间，细胞质RNA在前导电解质和尾部电解质之间的ITP界面处积累，并且可以随后被提取而不含核DNA。该方法可以在完全自动化该过程的所有步骤的微流体装置中进行。

5. 发明申请

WO2013154943A9 PLASMA TREATMENT FOR DNA BINDING 审中-公开
标题翻译：用于DNA结合的等离子体处理
公开(公告)号：WO2013154943A9
公开(公告)日：2015-01-08
申请号：PCT/US2013035477
申请日：2013-04-05
申请人： APDN BVI INC
发明人： BERRADA ABDELKRIM , LIANG MINGHWA BENJAMIN , HAYWARD JAMES ARTHUR , JUNG LAWRENCE
IPC分类号： G01N1/28 , H05H1/00
CPC分类号： C12N15/101 , B01J19/08 , B01J19/088 , B01J2219/0879 , B01J2219/0894 , B01J2219/24 , C12N15/1006 , C12Q1/6806 , G01N27/447 , G01N27/44791
摘要： The invention provides a composition including DNA bonded to a plasma-treated surface, the plasma can be any suitable plasma, such as an argon plasma, a compressed air plasma, a flame-based plasma or a vacuum plasma. Surfaces treatable by the methods of the invention include ceramic, metal, fabric and organic polymer surfaces. The DNA can be any DNA, such as a marker DNA, which can be linear or circular, single-stranded or double stranded and from about 25 bases to about 10,000 bases in length. Also provided is a method of binding DNA to a surface, including the steps of exposing the surface to a plasma to produce a plasma-treated surface; and applying DNA to the plasma-treated surface to produce surface bound DNA on the treated surface. A system for binding DNA to a surface is also disclosed, the system includes a plasma generator adapted to treating a surface with a plasma to produce a plasma-treated surface; and an applicator containing DNA adapted to applying DNA to the plasma-treated surface to produce surface bound DNA on the plasma-treated surface.
摘要翻译：本发明提供了包含结合到等离子体处理表面的DNA的组合物，等离子体可以是任何合适的等离子体，例如氩等离子体，压缩空气等离子体，基于火焰的等离子体或真空等离子体。可通过本发明的方法处理的表面包括陶瓷，金属，织物和有机聚合物表面。 DNA可以是任何DNA，例如标记DNA，其可以是线性或环状，单链或双链，并且长度为约25碱基至约10,000碱基。还提供了将DNA结合到表面的方法，包括将表面暴露于等离子体以产生等离子体处理的表面的步骤; 并将DNA施加到等离子体处理的表面上以在被处理的表面上产生表面结合的DNA。还公开了将DNA结合到表面的系统，该系统包括适于用等离子体处理表面以产生等离子体处理的表面的等离子体发生器; 以及包含适于将DNA施加到等离子体处理的表面以在等离子体处理的表面上产生表面结合的DNA的DNA的施加器。

6. 发明申请

WO2014152027A1 MANUFACTURING METHODS FOR PRODUCTION OF RNA TRANSCRIPTS 审中-公开
标题翻译：生产RNA转录物的制造方法
公开(公告)号：WO2014152027A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/026835
申请日：2014-03-13
申请人： MODERNA THERAPEUTICS, INC.
发明人： BANCEL, Stephane , ISSA, William, Joseph , AUNINS, John, Grant , CHAKRABORTY, Tirtha
IPC分类号： C12P19/34 , C12P19/30 , C12P19/26
CPC分类号： C12P19/34 , C12N15/101 , C12Q1/6865 , C12Q2525/173 , C12Q2565/629
摘要： Described are methods for production of RNA transcripts using a non-amplified, linearized DNA tempate in an in vitro transcription reaction. Enzymatic 5 ' capping and oligo dT purification can also be included in the methods.
摘要翻译：描述了在体外转录反应中使用非扩增的线性化DNA温和物质生产RNA转录物的方法。酶法5'封端和寡聚dT纯化也可以包括在这些方法中。

7. 发明申请

WO2014072366A1 METHOD FOR LYSING A FIXED BIOLOGICAL SAMPLE 审中-公开
标题翻译：用于固定固定生物样品的方法
公开(公告)号：WO2014072366A1
公开(公告)日：2014-05-15
申请号：PCT/EP2013/073204
申请日：2013-11-07
申请人： QIAGEN GMBH
发明人： GROSSHAUSER, Gerd , HIMMELREICH, Ralf
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101 , C12N15/1013
摘要： The present invention provides a method for lysing a fixed biological sample wherein the obtained lysate is suitable for being directly used in a nucleic acid analysis method, comprising: a) contacting the fixed biological sample with an aqueous lysis composition thereby providing a lysis mixture; b) heating the lysis mixture at ≥ 85° C to provide a lysate; wherein inhibitors of the subsequent nucleic acid analysis method are depleted by i) contacting the fixed biological sample in step a) with at least one compound which prevents or reduces the inhibition of the subsequent nucleic acid analysis method and/or ii) binding inhibitors to a solid support having an anionic surface. The method is rapid, efficient, does not require the use of chaotropic salts and does not require a prior purification of the nucleic acids prior to performing the analytical method. A portion of the obtained lysate can be used directly e.g. in an amplification reaction.
摘要翻译：本发明提供了一种用于裂解固定的生物样品的方法，其中所获得的裂解物适于直接用于核酸分析方法，其包括：a）使固定的生物样品与含水裂解组合物接触，从而提供裂解混合物; b）在≥85℃加热裂解混合物以提供裂解物; 其中随后的核酸分析方法的抑制剂通过以下步骤来消耗：i）使步骤a）中的固定生物样品与至少一种预防或减少后续核酸分析方法的抑制的化合物和/或ii）将抑制剂结合至具有阴离子表面的固体支持物。该方法快速，有效，不需要使用离液盐，并且在进行分析方法之前不需要先前纯化核酸。所获得的裂解物的一部分可以直接使用。在扩增反应中。

8. 发明申请

WO2012062753A9 METHOD AND DEVICE FOR ISOLATING AND PURIFYING DOUBLE-STRANDED NUCLEIC ACIDS 审中-公开
标题翻译：分离和清洗方法和装置的双链核酸
公开(公告)号：WO2012062753A9
公开(公告)日：2013-06-27
申请号：PCT/EP2011069644
申请日：2011-11-08
申请人： QIAGEN GMBH , SINGER THORSTEN , WEDLER HOLGER
发明人： SINGER THORSTEN , WEDLER HOLGER
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/101 , B01D15/34 , B01D15/3828 , C07H1/08
摘要： The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.
摘要翻译：本发明是用于双链核酸的分离和/或纯化的色谱装置中，来自这样的核酸与单链核酸，寡核苷酸，单核苷酸，盐和/或其它杂质的混合物，以及于色谱分离和/的方法优选双链DNA 或其清洁和试剂盒为此。

9. 发明申请

WO2013083260A1 RECOVERY OF A BIOMOLECULE COMPRISING AQUEOUS PHASE FROM A MULTIPHASIC MIXTURE 审中-公开
标题翻译：从多元混合物中回收包含水相的生物分子
公开(公告)号：WO2013083260A1
公开(公告)日：2013-06-13
申请号：PCT/EP2012/004987
申请日：2012-12-04
申请人： QIAGEN GMBH , HUCKLENBROICH, Jörg
发明人： HUCKLENBROICH, Jörg , NARZ, Franz
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1017 , C12N15/1003 , C12N15/1006 , C12N15/101 , C12Q1/6806 , C12Q2521/537 , C12Q2527/125 , C12Q2565/137
摘要： The present invention relates to a method for recovering an aqueous phase comprising biomolecules dissolved therein from a multiphasic mixture, comprising at least said aqueous phase and a further liquid phase which is immiscible with said aqueous phase wherein said further liquid phase comprises at least one hydrocarbon compound. The invention further relates to the use of a hydrophilic filtering material, a device comprising such a filtering material or a kit comprising said device for recovering an aqueous phase comprising biomolecules dissolved therein from a mixture of said aqueous phase and at least one hydrocarbon compound comprising further liquid phase which is immiscible with said aqueous phase.
摘要翻译：本发明涉及一种从多相混合物中回收其中溶解于其中的生物分子的水相的方法，所述多相混合物至少包含所述水相和与所述水相不混溶的另一液相，其中所述另外的液相包含至少一种烃化合物。本发明还涉及亲水过滤材料的使用，包含这种过滤材料的装置或包含所述装置的试剂盒，该试剂盒包括用于从所述水相和至少一种烃化合物的混合物中回收包含其中溶解于其中的生物分子的水相，与所述水相不混溶的液相。

10. 发明申请

WO2012116945A3 LIQUID PHASE SEPARATION OF PLASMID DNA ISOFORMS AND TOPOISOMERS 审中-公开
标题翻译： PLASMID DNA ISOFORMS和TOPOISOMERS的液相分离
公开(公告)号：WO2012116945A3
公开(公告)日：2012-12-13
申请号：PCT/EP2012053241
申请日：2012-02-27
申请人： BOEHRINGER INGELHEIM RCV GMBH , LAEMMERHOFER MICHAEL , LINDNER WOLFGANG , MAHUT MAREK
发明人： LAEMMERHOFER MICHAEL , LINDNER WOLFGANG , MAHUT MAREK
IPC分类号： B01D15/38 , C12N15/10
CPC分类号： B01D15/363 , B01J20/286 , B01J20/3263 , B01J41/20 , B01J2220/82 , C12N15/101
摘要： The invention relates to the use of material comprising a carbamoyl-decorated anion-exchanger ligandwherein a cationic group (C), and a hydrogen donor group (N-H), are connected by a spacer with a length between 3 to 5 atoms, immobilized onto or embedded into a heterogeneous matrix, for the separation of isoforms, topoisomers, oligomeric and multimeric forms of plasmid DNA (p DNA).
摘要翻译：本发明涉及包含在阳离子基团（C）和氢供体基团（NH）中的氨基甲酰基修饰的阴离子交换配体的材料的使用，其通过长度在3至5个原子之间的间隔物连接，固定在或嵌入到异质基质中，用于分离同种型，拓扑异构体，寡聚和多聚体形式的质粒DNA（p DNA）。

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