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1. 发明申请

WO1997004126A1 METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES BY TRANSLOCATION USING CHIMERIC PRIMERS 审中-公开
标题翻译：通过使用CHIMERIC PRIMERS进行转录来放大核酸序列的方法
公开(公告)号：WO1997004126A1
公开(公告)日：1997-02-06
申请号：PCT/FR1996001166
申请日：1996-07-24
申请人： BIO MERIEUX , CLEUZIAT, Philippe , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6844 , C12Q2537/113 , C12Q2527/101 , C12Q2521/313 , C12Q2531/119 , C12Q2525/121
摘要： A target nucleic acid sequence (12) may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer (A1, A2) that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridising with a 3'-terminal segment of the target and a DNA-type segment capable of hybridising with a segment adjacent to said 3'-terminal segment of the target, and a DNA- or RNA-type primer B1 capable of hybridising with said 3'-terminal segment of the target. A cyclic amplification is achieved that may be implemented isothermally on the basis of either a DNA or an RNA target even when the terminals are not defined.
摘要翻译：可以通过使用包含5'至3'方向的嵌合引物（A1，A2）在包含DNA聚合酶，链转位和RNAse H活性的酶系统存在下扩增靶核酸序列（12）能够与靶标的3'末端区段杂交的RNA型区段和能够与靶标的3'-末端区段相邻的区段杂交的DNA型区段，以及DNA型或RNA型区段能够与目标的所述3'-末端区段杂交的引物B1。实现了循环扩增，其可以基于DNA或RNA靶标等温地实现，即使在末端未定义时也是如此。

2. 发明申请

WO1997045202A1 SUPERPARAMAGNETIC MONODISPERSED PARTICLES 审中-公开
标题翻译：超级粒子单体颗粒
公开(公告)号：WO1997045202A1
公开(公告)日：1997-12-04
申请号：PCT/FR1997000912
申请日：1997-05-23
申请人： BIO MERIEUX , ELAISSARI, Abdelhamid , PICHOT, Christian , MANDRAND, Bernard , SAUZEDDE, Florence
发明人： BIO MERIEUX
IPC分类号： B03C01/01
CPC分类号： H01F1/37 , B03C1/01 , G01N33/5375 , G01N33/5434 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , H01F1/44 , Y10S436/806 , Y10S436/824 , Y10T428/2982 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998 , Y10T436/24
摘要： The invention discloses superparamagnetic monodispersed particles, their method of preparation and their uses. These particles comprise: a core of a first polymer, an internal layer of a second polymer coating the core, and in which is distributed a magnetic material, and an external layer of a third polymer coating the magnetic layer, and capable of interacting with at least one biological molecule, at least the second polymer being heat sensitive and having a predetermined lower critical solubility temperature (LCST) of 15 to 65 DEG C.
摘要翻译：本发明公开了超顺磁单分散颗粒，其制备方法及其用途。这些颗粒包括：第一聚合物的芯，涂覆芯的第二聚合物的内层，其中分布有磁性材料，以及涂覆磁性层的第三聚合物的外层，并且能够与至少一个生物分子，至少第二聚合物是热敏感的并具有15至65℃的预定的较低临界溶解温度（LCST）。

3. 发明申请

WO1995003426A2 METHOD FOR NUCLEIC ACID AMPLIFICATION BY TRANSCRIPTION USING DISPLACEMENT, AND REAGENTS AND KIT THEREFOR 审中-公开
标题翻译：通过使用位移转录进行核酸扩增的方法及其试剂和试剂盒
公开(公告)号：WO1995003426A2
公开(公告)日：1995-02-02
申请号：PCT/FR1994000935
申请日：1994-07-26
申请人： BIO MERIEUX , CLEUZIAT, Philippe , GUILLOU-BONNICI, Françoise , MALLET, François , LEVASSEUR, Pierre
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6853 , C12Q1/6844 , C12Q1/6846 , C12Q2531/119 , C12Q2521/107 , C12Q2521/101 , C12Q2537/1373
摘要： A method for amplifying a target nucleic acid sequence by providing a polynucleotide such as VII including the sequence to be amplified as well as an RNA polymerase promoter sequence, and contacting said polynucleotide, in the presence of a system having RNA polymerase activity, RNA-dependent DNA polymerase activity, and DNA-dependent DNA polymerase activity, and capable of strand displacement, with a set of primers includinf a primer such as A capable of hybridising with a segment complementary to a portion of the sequence to be amplified, wherein A includes an upstream RNA polymerase promoter sequence then an arbitrary sequence, a primer such as D contains said RNA polymerase promoter sequence, and a primer such as C contains said arbitrary sequence. The elongation product of D is displaced by the elongation product of C, and the elongation product of D is thus obtained in the form of single-stranded VIII. Hybridisation of A on VIII followed by elongation forms double-stranded product X which can undergo transcription, with amplification, to give single-stranded RNA VII bis. An analogous system of 3 primers B, E and F similary produces single-stranded RNA VII from VII bis. Hence the amplification method, which can operate isothermally, is cyclic.
摘要翻译：通过提供包括待扩增的序列的多核苷酸，以及RNA聚合酶启动子序列，并且在具有RNA聚合酶活性的系统存在下使所述多核苷酸与RNA聚合酶活性相接触来扩增靶核酸序列的方法 DNA聚合酶活性和DNA依赖性DNA聚合酶活性，并且能够使用一组引物，包括能够与与待扩增的序列的一部分互补的区段杂交的引物如A的引物，其中A包括上游RNA聚合酶启动子序列然后是任意序列，引物如D含有所述RNA聚合酶启动子序列，引物如C包含所述任意序列。 D的伸长率由C的伸长率移位，因此以单链VIII的形式得到D的伸长率。 A对VIII的杂交，然后延伸形成双链产物X，其可经历转录，扩增，得到单链RNA VII bis。 3个引物B，E和F的类似系统同样产生VII bis的单链RNA VII。因此，可以等温操作的扩增方法是循环的。

4. 发明申请

WO1997046318A1 SINGLE-USE ANALYSIS CARD COMPRISING A LIQUID FLOW CHANNEL 审中-公开
标题翻译：包含液体流通道的单次使用分析卡
公开(公告)号：WO1997046318A1
公开(公告)日：1997-12-11
申请号：PCT/FR1997001020
申请日：1997-06-09
申请人： BIO MERIEUX , COLIN, Bruno , JARAVEL, Cécile , CLEUZIAT, Philippe
发明人： BIO MERIEUX
IPC分类号： B01L03/00
CPC分类号： B01L3/502
摘要： The invention features an analysing device (1) comprising a body (2) in which are arranged or provided: an intake aperture (3) for a starting liquid sample, a liquid flow circuit (5) comprising at least one operating cell (6) for a processed liquid sample, obtained from all or part of the original sample, communicating with the said intake aperture (3), the said flow circuit defining, in at least two dimensions of the card, one determined geometric line, such that any alteration in the card orientation in a three-dimensional reference frame, causes the liquid to flow under gravity only, from one part of the said circuit to another,for instance from one side or another of the operating cell, (6) characterised in that, the flow circuit (5) is continuous, and looped on itself between the said aperture (3) and the said operating cell (6).
摘要翻译：本发明的特征在于一种分析装置（1），包括其中布置或提供的主体（2）：用于起始液体样品的进气孔（3），包括至少一个操作室（6）的液体流动回路（5）对于从全部或部分原始样品获得的经处理的液体样品，与所述进气孔（3）连通，所述流动回路在卡的至少两个维度上限定一个确定的几何线，使得任何改变在三维参考框架中的卡片取向中，使得液体仅在重力下从所述电路的一部分流向另一部分，例如从操作单元的一侧或另一侧流出，其特征在于，流动回路（5）是连续的，并且环绕在所述孔（3）和所述操作室（6）之间。

5. 发明申请

WO1995021859A1 CYTOTOXIC FACTOR ASSOCIATED WITH DISSEMINATED SCLEROSIS, ITS DETECTION AND QUANTIFICATION 审中-公开
标题翻译：与感染性SCLEROSIS相关的细胞因子，其检测和定量
公开(公告)号：WO1995021859A1
公开(公告)日：1995-08-17
申请号：PCT/FR1995000178
申请日：1995-02-15
申请人： BIO MERIEUX , PERRON, Hervé , DOBRANSKY, Tomas , RIEGER, François , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C07K14/47
CPC分类号： C07K14/4713 , A61K38/00 , G01N33/5091 , Y10S424/16 , Y10S435/964 , Y10S436/827 , Y10S436/828
摘要： Gliotoxic factor, in isolated or purified condition, characterized in that it has a toxic activity on animal or human astrocytic cells resulting in a cytomorphological disorganization of their network of intermediary filaments and/or a degradation of the proteins of said intermediary filaments and/or cell death particularly, by apoptosis.
摘要翻译：在分离或纯化条件下的胶质毒性因子，其特征在于其对动物或人星形细胞细胞具有毒性活性，导致其中间丝的网络的细胞形态学紊乱和/或所述中间丝和/或细胞的蛋白质的降解死亡特别是凋亡。

6. 发明申请

WO1993002213A1 PROBE SYSTEM FOR PERFORMING HLA DR TYPING AND TYPING METHOD USING SAID PROBES 审中-公开
标题翻译：用于执行HLA DR类型的探测系统和使用所述探测器的类型方法
公开(公告)号：WO1993002213A1
公开(公告)日：1993-02-04
申请号：PCT/FR1992000702
申请日：1992-07-17
申请人： BIO MERIEUX , ALLIBERT, Patrice, André , GROS, Philippe , MACH, Bernard, François , MANDRAND, Bernard, Fabien , TIERCY, Jean-Marie
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6881 , C12Q2600/156
摘要： A set of nucleotide probes for determining HLA DR types and/or related types by means of commonly used typing techniques using oligonucleotides, wherein said set of probes includes at least the following nucleotide probes (the underligned portion represents the optimal sequence) (I). Said probes may be labelled, covalently coupled to a ligand which facilitates their attachment to a solid support, or attached to a solid support. The use of the probes in a HLA DR typing method, e.g. as capture probes according to the sandwich technique, is also described.

7. 发明申请

WO1998011439A1 METHOD FOR DETECTING AND/OR QUANTIFYING A GLIOTOXIC FACTOR 审中-公开
标题翻译：检测和/或定量GLIOTOXIC因子的方法
公开(公告)号：WO1998011439A1
公开(公告)日：1998-03-19
申请号：PCT/FR1997001620
申请日：1997-09-12
申请人： BIO MERIEUX , MALCUS-VOCANSON, Carine , PERRON, Hervé , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： G01N33/564
CPC分类号： G01N33/564 , G01N33/5014 , G01N33/6896
摘要： The invention proposes a method for detecting and/or quantifying, in a biological sample a cytotoxic factor, in particular a gliotoxic factor, with respect to adherent target cells, in particular macroglial cells, the toxicity of which causes the death by apoptosis of said cells. The method consists in providing an initial fraction of said sample, optionally enriched with said toxic factor by previous treatment, incubating said initial toxic factor with a reference culture medium comprising adherent target cells, and detecting and/or quantifying the adherent target cells killed by apoptosis, by flux cytometry, at least one direct or indirect characteristic associated with the apoptotic adherent cells of the whole or part of the incubated medium, which, if it is present and/or is quantified, qualifies the sample as positive, i.e. as containing said toxic factor. The initial biological sample is preferably a urine specimen.
摘要翻译：本发明提出了一种用于在生物样品中检测和/或定量细胞毒性因子，特别是胶质毒性因子的方法，涉及粘附靶细胞，特别是大胶质细胞，其毒性通过所述细胞的凋亡导致死亡。该方法包括提供所述样品的初始级分，任选地通过先前的处理富集所述毒性因子，将所述初始毒性因子与包含贴壁靶细胞的参照培养基孵育，并检测和/或定量由细胞凋亡杀死的贴壁靶细胞通过流式细胞术，与整个或部分培养的培养基的凋亡贴壁细胞相关的至少一种直接或间接特征，如果存在和/或定量，则将样品鉴定为阳性，即含有所述毒性因素。初始生物样品优选为尿样。

8. 发明申请

WO1995021256A1 MSRV1 VIRUS AND MSRV2 PATHOGENIC AND/OR INFECTIOUS AGENT ASSOCIATED WITH MULTIPLE SCLEROSIS, NUCLEIC ACID COMPONENTS AND APPLICATIONS OF SAME 审中-公开
标题翻译： MSRV1病毒和MSRV2致病和/或感染代谢相关的多发性糖尿病，核酸成分及其应用
公开(公告)号：WO1995021256A1
公开(公告)日：1995-08-10
申请号：PCT/FR1995000142
申请日：1995-02-06
申请人： BIO MERIEUX , PERRON, Hervé , MALLET, François , MANDRAND, Bernard , BEDIN, Frédéric , BESEME, Frédéric
发明人： BIO MERIEUX
IPC分类号： C12N15/48
CPC分类号： C07K14/005 , A61K38/00 , C12N7/00 , C12N2740/10021 , C12N2740/10022 , Y10S435/912
摘要： Composition comprising two pathogenic and/or infectious agents associated with multiple sclerosis, namely, a first agent comprising a human virus with reverse transcriptase activity, and related to a family of endogenous retroviral elements, or a variant thereof, and a second agent, or a variant thereof, wherein the two said pathogenic and/or infectious agents originate from the same viral strain selected from the strains respectively designated as POL-2, registered on 22.07.1992 with ECACC under accession number V92072202, and MS7PG, registered on 08.01.93 with ECACC under accession number V93010816, or from their variant strains.
摘要翻译：包含与多发性硬化症相关的两种致病和/或感染因子的组合物，即包含具有逆转录酶活性的人病毒并与内源性逆转录病毒元件家族或其变体相关的第一药剂和第二药剂，或其中所述两种病原体和/或感染因子源自选自2003年22.07.1992号登记号为V92072202的ECACC分别指定为POL-2的菌株和08.01.93登记的MS7PG的相同病毒株 ECACC保藏号为V93010816，或其变体菌株。

9. 发明申请

WO1991019812A1 METHOD FOR DETECTING A NUCLEOTIDE SEQUENCE BY SANDWICH HYBRIDIZATION 审中-公开
标题翻译：通过三聚体杂化检测核苷酸序列的方法
公开(公告)号：WO1991019812A1
公开(公告)日：1991-12-26
申请号：PCT/FR1991000468
申请日：1991-06-11
申请人： BIO MERIEUX , CROS, Philippe , ALLIBERT, Patrice , MALLET, François , MABILAT, Claude , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6883 , C12Q1/6813 , C12Q1/6834 , C12Q1/6886 , C12Q1/689 , C12Q1/703 , C12Q1/708
摘要： A method for detecting a single-stranded nucleotide sequence in a sample which is known or thought to contain it, using sandwich hybridization comprising the step of incubating the sample with a capture probe which is passively fixed to a solid support and with a detector probe which is labelled with a non-radioactive marker. The capture and detector probes can hybridize with two non-overlapping regions respectively of the target nucleotide sequence being sought. The incubation step is followed by a washing step for removing reagents which have not been fixed by hybridization. The method is characterized in that the capture probe contains 9-30 nucleotides and is fixed to said solid support which is made of a material based on a hydrophobic compound. The method can be used particularly for diagnosing infectious or genetic diseases and for cell typing.

10. 发明申请

WO1997035031A1 NUCLEOTIDE SEQUENCE DETECTION WITH SIGNAL AMPLIFICATION 审中-公开
标题翻译：核苷酸序列检测与信号放大
公开(公告)号：WO1997035031A1
公开(公告)日：1997-09-25
申请号：PCT/FR1997000483
申请日：1997-03-19
申请人： BIO MERIEUX , DELAIR, Thierry , ELAISSARI, Abdelhamid , CHARLES, Marie-Hélène , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6834 , C12Q1/682
摘要： A kit for detecting a nucleotide sequence of interest with signal amplification, containing a labelled nucleotide probe and a reagent essentially including a suspension of particles on which at least one series of oligonucleotide units is immobilised. Each of the series of oligonucleotide units, which are all identical, includes, at least, a nucleotide sequence hybridizable with said sequence of interest and a nucleotide sequence hybridizable with said probe. Said reagent contains more than 10 such oligonucleotide units per particle. The kit is particularly useful for diagnosing genetic diseases or determining pathogens such as bacteria, viruses, fungi or parasites.

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