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1. 发明申请

WO1998011439A1 METHOD FOR DETECTING AND/OR QUANTIFYING A GLIOTOXIC FACTOR 审中-公开
标题翻译：检测和/或定量GLIOTOXIC因子的方法
公开(公告)号：WO1998011439A1
公开(公告)日：1998-03-19
申请号：PCT/FR1997001620
申请日：1997-09-12
申请人： BIO MERIEUX , MALCUS-VOCANSON, Carine , PERRON, Hervé , MANDRAND, Bernard
发明人： BIO MERIEUX
IPC分类号： G01N33/564
CPC分类号： G01N33/564 , G01N33/5014 , G01N33/6896
摘要： The invention proposes a method for detecting and/or quantifying, in a biological sample a cytotoxic factor, in particular a gliotoxic factor, with respect to adherent target cells, in particular macroglial cells, the toxicity of which causes the death by apoptosis of said cells. The method consists in providing an initial fraction of said sample, optionally enriched with said toxic factor by previous treatment, incubating said initial toxic factor with a reference culture medium comprising adherent target cells, and detecting and/or quantifying the adherent target cells killed by apoptosis, by flux cytometry, at least one direct or indirect characteristic associated with the apoptotic adherent cells of the whole or part of the incubated medium, which, if it is present and/or is quantified, qualifies the sample as positive, i.e. as containing said toxic factor. The initial biological sample is preferably a urine specimen.
摘要翻译：本发明提出了一种用于在生物样品中检测和/或定量细胞毒性因子，特别是胶质毒性因子的方法，涉及粘附靶细胞，特别是大胶质细胞，其毒性通过所述细胞的凋亡导致死亡。该方法包括提供所述样品的初始级分，任选地通过先前的处理富集所述毒性因子，将所述初始毒性因子与包含贴壁靶细胞的参照培养基孵育，并检测和/或定量由细胞凋亡杀死的贴壁靶细胞通过流式细胞术，与整个或部分培养的培养基的凋亡贴壁细胞相关的至少一种直接或间接特征，如果存在和/或定量，则将样品鉴定为阳性，即含有所述毒性因素。初始生物样品优选为尿样。

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