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1. 发明申请

WO2008106678A1 DROPLET ACTUATOR STRUCTURES 审中-公开
标题翻译： DROPLET执行机构结构
公开(公告)号：WO2008106678A1
公开(公告)日：2008-09-04
申请号：PCT/US2008/055648
申请日：2008-03-03
申请人： ADVANCED LIQUID LOGIC, INC. , PAMULA, Vamsee, K. , POLLACK, Michael, G. , SRINIVASAN, Vijay , PAIK, Phil
发明人： PAMULA, Vamsee, K. , POLLACK, Michael, G. , SRINIVASAN, Vijay , PAIK, Phil
IPC分类号： B41J2/045
CPC分类号： B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
摘要： Approaches to configuring and wiring electrodes in a droplet actuator are provided. Droplet actuators employing the designs of the invention are useful for conducting a variety of droplet operations. In one set of embodiments, the droplet actuator of the invention includes various single-layer wiring configurations for mitigating the constraints and drawbacks that are associated with single-layer designs, such as wireability constraints, limited mechanisms for performing droplet operations, electrostatic interference from wires, and any combinations thereof. In another set of embodiments, the droplet actuator of the invention includes a reference electrode that is situated on one substrate that is separated by a gap from a second substrate and one or more control electrodes that are situated on the second substrate. The control electrodes may be placed such that the second substrate is interposed between the control electrodes and the first substrate.
摘要翻译：提供了在液滴致动器中配置和布线电极的方法。采用本发明设计的液滴致动器可用于进行各种液滴操作。在一组实施例中，本发明的液滴致动器包括用于减轻与单层设计相关联的约束和缺点的各种单层布线配置，例如线性约束，用于执行液滴操作的有限机构，来自线的静电干扰，及其任何组合。在另一组实施例中，本发明的液滴致动器包括参考电极，该参考电极位于一个衬底上，其间隔开第二衬底的间隙和位于第二衬底上的一个或多个控制电极。控制电极可以被放置成使得第二衬底插入在控制电极和第一衬底之间。

2. 发明申请

WO2008051310A2 DROPLET MANIPULATION SYSTEMS 审中-公开
标题翻译： DROPLET操作系统
公开(公告)号：WO2008051310A2
公开(公告)日：2008-05-02
申请号：PCT/US2007/011298
申请日：2007-05-09
申请人： ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY , PAIK, Philip, Y. , BRAFFORD, Keith, R. , STURMER, Ryan, A. , SMITH, Gregory, F. , POLLACK, Michael, G. , PAMULA, Vamsee, K. , WEST, Richard, M. , SRINIVASAN, Vijay
发明人： PAIK, Philip, Y. , BRAFFORD, Keith, R. , STURMER, Ryan, A. , SMITH, Gregory, F. , POLLACK, Michael, G. , PAMULA, Vamsee, K. , WEST, Richard, M. , SRINIVASAN, Vijay
IPC分类号： B01D57/00
CPC分类号： B01F13/0076 , B01F13/0071 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0673 , B01L2200/14 , B01L2200/143 , B01L2200/16 , B01L2300/027 , B01L2300/0819 , B01L2300/089 , B01L2300/1827 , B01L2400/0421 , B01L2400/0427 , B01L2400/0448 , G01N33/49 , G01N2035/1034
摘要： The present invention relates to systems and methods for controlling droplet movements on a droplet microactuator and a droplet microactuator device, system and method for processing and/or analyzing samples. According to one aspect, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, and a display device displaying a user interface electronically coupled to the controller, wherein the system is programmed and configured to permit a user to effect a droplet manipulation by interacting with the user interface. According to another aspect, a sample analyzer is provided and includes an analyzer unit comprising electronic or optical receiving means, a cartridge comprising self-contained droplet handling capabilities, and a means for coupling the cartridge to the analyzer unit which aligns electronic and/or optical outputs from the cartridge with electronic or optical receiving means on the analyzer unit.
摘要翻译：本发明涉及用于控制液滴微致动器上的液滴运动的系统和方法以及液滴微致动器装置，用于处理和/或分析样品的系统和方法。根据一个方面，提供了一种系统，其包括控制器，电子耦合到控制器的液滴微致动器以及显示电子耦合到控制器的用户界面的显示设备，其中系统被编程和配置为允许用户实现通过与用户界面交互来进行液滴操作。根据另一方面，提供一种样品分析器，其包括分析器单元，其包括电子或光学接收装置，包括独立液滴处理能力的盒，以及用于将盒耦合到分析器单元的装置，其将电子和/或光学对准在分析器单元上从带有电子或光学接收装置的墨盒输出。

3. 发明申请

WO2007120241A2 DROPLET-BASED BIOCHEMISTRY 审中-公开
标题翻译：基于DROPLET的生物化学
公开(公告)号：WO2007120241A2
公开(公告)日：2007-10-25
申请号：PCT/US2006/047486
申请日：2006-12-11
申请人： ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY , POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay , PAIK, Philip, Y. , ECKHARDT, Allen, E. , FAIR, Richard, B.
发明人： POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay , PAIK, Philip, Y. , ECKHARDT, Allen, E. , FAIR, Richard, B.
IPC分类号： B01D57/00
CPC分类号： B01L3/502792 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/6869 , G01N33/49 , C12Q2565/629 , C12Q2565/301
摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using discrete droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting biochemical reactions, such as the reagents described for use in nucleic acid amplification protocols, affinity-based assay protocols, sequencing protocols, and protocols for analyses of biological fluids.
摘要翻译：液滴微致动器技术领域本发明涉及液滴微致动器以及使用液滴微致动器用于使用离散液滴执行各种协议的系统，设备和方法。本发明包括具有装载有用于进行生物化学反应的试剂的一个或多个输入储存器的液滴微致动器或液滴微致动器系统，例如用于核酸扩增方案的所述试剂，基于亲和性的测定方案，测序方案和用于分析的方案的生物液体。

4. 发明申请

WO2007123908A2 DROPLET-BASED MULTIWELL OPERATIONS 审中-公开
标题翻译：基于DROPLET的多媒体操作
公开(公告)号：WO2007123908A2
公开(公告)日：2007-11-01
申请号：PCT/US2007/009379
申请日：2007-04-18
申请人： ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY , POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay
发明人： POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay
IPC分类号： C12M3/00
CPC分类号： B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
摘要： The present invention relates to droplet-based multiwell operations. One aspect of the present invention relates to droplet-based protein crystallization including embodiments related to a screening chip, an optimization chip, and a method of testing conditions for growing a crystal. Another aspect of the present invention relates to a droplet-based multiwell plate including embodiments related to a substrate including one or more input ports, a regular array of processing wells, and a network of droplet transport pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Yet another aspect of the present invention relates to a droplet-microactuator stamping platform including embodiments related to a stamping device comprising a droplet microactuator and a system regarding the same.
摘要翻译：本发明涉及基于液滴的多井操作。本发明的一个方面涉及基于液滴的蛋白质结晶，其包括与筛选芯片相关的实施方案，优化芯片以及测试用于生长晶体的条件的方法。本发明的另一方面涉及一种基于液滴的多孔板，其包括与包括一个或多个输入端口的基板相关的实施例，处理孔的规则阵列，以及从每一个提供直接或间接液滴传输的液滴传输路径网络的输入端口连接到一个或多个处理孔中的每一个。本发明的另一方面涉及一种液滴微致动器冲压平台，其包括与包括液滴微致动器的冲压装置有关的实施例以及与其相关的系统。

5. 发明申请

WO2007120240A2 DROPLET-BASED PYROSEQUENCING 审中-公开
标题翻译：基于DROPLET的PYROSEQUENCING
公开(公告)号：WO2007120240A2
公开(公告)日：2007-10-25
申请号：PCT/US2006/047481
申请日：2006-12-11
申请人： ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY , THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , POLLACK, Michael, G. , PAMULA, Vamsee, K. , ALLEN, Dwayne, J. , FAIR, Richard, B. , GRIFFIN, Peter
发明人： POLLACK, Michael, G. , PAMULA, Vamsee, K. , ALLEN, Dwayne, J. , FAIR, Richard, B. , GRIFFIN, Peter
IPC分类号： B01D57/00
CPC分类号： B01L3/502792 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/6869 , G01N33/49 , C12Q2565/629 , C12Q2565/301
摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.
摘要翻译：液滴微致动器技术领域本发明涉及液滴微致动器以及采用液滴微致动器来使用液滴执行各种协议的系统，装置和方法。本发明包括液滴微致动器或液滴微致动器系统，其具有一个或多个输入储存器，其中装载有用于进行测序方案的试剂，例如用于进行焦磷酸测序方案的试剂。本发明还包括液滴微致动器或液滴微致动器系统，其具有装载有用于进行焦磷酸测序方案的样品的一个或多个输入储存器。

6. 发明申请

WO2011106314A2 METHOD OF MAKING NUCLEIC ACID LIBRARIES 审中-公开
标题翻译：制备核酸图谱的方法
公开(公告)号：WO2011106314A2
公开(公告)日：2011-09-01
申请号：PCT/US2011/025711
申请日：2011-02-22
申请人： ADVANCED LIQUID LOGIC, INC. , POLLACK, Michael, G. , ECKHARDT, Allen, E. , SUDARSAN, Arjun , SRINIVASAN, Vijay , ROUSE, Jeremy , YI, Uichong , TROTTA, Nicholas , DHOPESHWARKAR, Rahul , THWAR, Prasanna , SMITH, Gregory, F. , NORTON, Scott , LINNARTZ, Peter
发明人： POLLACK, Michael, G. , ECKHARDT, Allen, E. , SUDARSAN, Arjun , SRINIVASAN, Vijay , ROUSE, Jeremy , YI, Uichong , TROTTA, Nicholas , DHOPESHWARKAR, Rahul , THWAR, Prasanna , SMITH, Gregory, F. , NORTON, Scott , LINNARTZ, Peter
IPC分类号： C12N15/10 , C40B50/08 , C40B40/06 , C07H21/00 , C12Q1/68
CPC分类号： C12N15/1075 , B01J19/0046 , C12N15/1006 , C40B40/08 , C40B50/06
摘要： A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.
摘要翻译：制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。

7. 发明申请

WO2004029585A1 METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES 审中-公开
标题翻译：通过电镀技术控制滴定液的方法和装置
公开(公告)号：WO2004029585A1
公开(公告)日：2004-04-08
申请号：PCT/US2003/012753
申请日：2003-04-24
申请人： DUKE UNIVERSITY , PAMULA, Vamsee, K. , POLLACK, Michael, G. , PAIK, Philip, Y. , REN, Hong , FAIR, Richard, B.
发明人： PAMULA, Vamsee, K. , POLLACK, Michael, G. , PAIK, Philip, Y. , REN, Hong , FAIR, Richard, B.
IPC分类号： G01N1/00
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： In a method for sampling a continuous liquid flow, the liquid flow is supplied to a surface along an input flow path. The liquid flow is sampled by forming a sample droplet from a portion of the liquid flow. The sample droplet is moved along an analysis flow path to a processing area of the surface, where the sample droplet is processed. Discrete sample droplets are formed and moved using an electrowetting technique. A binary mixing apparatus and method are also provided. The apparatus comprises an array of electrodes, an electronic controller communicating with the electrodes, a sample droplet supply area communicating with the array, and an additive droplet supply area communicating with the array. The electronic controller alternately energizes and de-energizes selected electrodes to carry out droplet-to-droplet binary mixing operations to obtain one or more droplets having a target mixing ratio.
摘要翻译：在对连续液体流进行取样的方法中，液体流被输送到沿输入流路的表面。通过从液体流的一部分形成样品液滴来取样液体流。样品液滴沿着分析流动路径移动到表面的处理区域，在该处理区域处理样品液滴。使用电润湿技术形成和移动离散样品液滴。还提供了二元混合装置和方法。该装置包括电极阵列，与电极连通的电子控制器，与该阵列连通的样品液滴供应区域以及与该阵列连通的附加液滴供应区域。电子控制器交替地激励和去激活所选择的电极以进行液滴到液滴的二进制混合操作以获得具有目标混合比的一个或多个液滴。

8. 发明申请

WO2004030820A2 METHODS AND APPARATUS FOR MANIPULATING DROPLETS BY ELECTROWETTING-BASED TECHNIQUES 审中-公开
标题翻译：通过电镀技术控制滴定液的方法和装置
公开(公告)号：WO2004030820A2
公开(公告)日：2004-04-15
申请号：PCT/US2003/012754
申请日：2003-04-24
申请人： DUKE UNIVERSITY , PAMULA, Vamsee, K. , POLLACK, Michael, G. , PAIK, Philip, Y. , REN, Hong , FAIR, Richard, B.
发明人： PAMULA, Vamsee, K. , POLLACK, Michael, G. , PAIK, Philip, Y. , REN, Hong , FAIR, Richard, B.
IPC分类号： B01L
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
摘要翻译：提供了一种用于操纵液滴的装置。该装置是单面电极设计，其中所有导电元件都包含在其上操作液滴的一个表面上。为了容纳待操作的液滴，可以与第一表面平行地设置另外的表面。通过执行基于电润湿的技术来操纵液滴，其中包含在或嵌入在第一表面中的电极被顺序地通电并以受控的方式断电。该装置能够进行多个液滴操纵过程，包括将两个液滴合并和混合在一起，将液滴分成两个或更多个液滴，通过从流动单独控制的液滴形成连续的液体流，以及液滴的迭代二进制或数字混合到获得所需的混合比。

9. 发明申请

WO2006081558A2 APPARATUSES AND METHODS FOR MANIPULATING DROPLETS ON A PRINTED CIRCUIT BOARD 审中-公开
标题翻译：在印刷电路板上操作液滴的装置和方法
公开(公告)号：WO2006081558A2
公开(公告)日：2006-08-03
申请号：PCT/US2006/003230
申请日：2006-01-30
申请人： DUKE UNIVERSITY , PAMULA, Vamsee, K. , POLLACK, Michael, G. , FAIR, Richard, B.
发明人： PAMULA, Vamsee, K. , POLLACK, Michael, G. , FAIR, Richard, B.
IPC分类号： G05D19/00
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.
摘要翻译：公开了在印刷电路板（PCB）上操纵液滴的装置和方法。通过将电位施加到PCB上限定的电极，使液滴在印刷电路板基板表面上被致动。还公开了使用焊接掩模作为液滴操作的电极绝缘体以及用于适应其它传统PCB层和用于液滴的微流体的材料的技术。

10. 发明申请

WO2014186697A2 DROPLET ACTUATOR FOR ELECTROPORATION AND TRANSFORMING CELLS 审中-公开
标题翻译：用于电镀和转化细胞的滴定致动器
公开(公告)号：WO2014186697A2
公开(公告)日：2014-11-20
申请号：PCT/US2014/038374
申请日：2014-05-16
申请人： ADVANCED LIQUID LOGIC, INC.
发明人： PUNNAMARAJU, Srikoundinya , SANDAHL, Melissa, A. , HARRINGTON, John, J. , ECKHARDT, Allen, E. , HUA, Zhishan , POLLACK, Michael, G.
IPC分类号： C12M1/42
CPC分类号： C12N15/87 , C12M33/00 , C12M35/02 , C12N13/00
摘要： The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.
摘要翻译：本发明提供了一种液滴致动器，其设计用于在液滴中对细胞进行电穿孔。本发明还提供了用于在液滴致动器上以液滴的细胞进行电穿孔的方法和系统。

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