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1. 发明申请

WO2017066485A1 DEVICES AND METHODS FOR EXTRACTION, SEPARATION AND THERMOCYCLING 审中-公开
标题翻译：用于提取，分离和热循环的装置和方法
公开(公告)号：WO2017066485A1
公开(公告)日：2017-04-20
申请号：PCT/US2016/056906
申请日：2016-10-13
申请人： LANDERS, James P. , DUVALL, Jacquelyn , LE ROUX, Delphine , ROOT, Brian , MILLS, Daniel , NELSON, Daniel A. , TSUEI, An-Chi , THOMPSON, Brandon L. , LI, Jingyi , BIRCH, Christopher
发明人： LANDERS, James P. , DUVALL, Jacquelyn , LE ROUX, Delphine , ROOT, Brian , MILLS, Daniel , NELSON, Daniel A. , TSUEI, An-Chi , THOMPSON, Brandon L. , LI, Jingyi , BIRCH, Christopher
IPC分类号： B01L7/00
CPC分类号： B01L3/50273 , B01L3/502753 , B01L7/52 , B01L2200/0621 , B01L2200/0647 , B01L2200/0684 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0803 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/0424 , G01N27/447
摘要： A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.
摘要翻译：在具有多个腔室和通道的微流体装置中提取，扩增和分离核酸的方法可包括a）将具有核酸的细胞引入微流体装置的第一腔室并使细胞在第一个室中进行裂解细胞的条件。该方法可以进一步包括：b）使第一室受到离心力，由此允许具有核酸的裂解物或其一部分通过微流体装置中的第一通道分配到第二室。该方法还可以包括c）将裂解物或其部分与用于扩增核酸的试剂组合，由此提供第二混合物。该方法还可以包括：d）使第二室受到离心力，从而使气体从第二混合物排出。

2. 发明申请

WO2014025500A3 THERMOSTABLE ENZYME-BASED EXTRACTIONS ON AN INTEGRATED MICROFLUIDIC CHIP FOR BIOLOGICAL ANALYSIS 审中-公开
公开(公告)号：WO2014025500A3
公开(公告)日：2014-02-13
申请号：PCT/US2013/050628
申请日：2013-07-16
申请人： MICROLAB HORIZON LLC , LANDERS, James, P. , KINNON, Paul , ROOT, Brian , SAUL, James, D.
发明人： LANDERS, James, P. , KINNON, Paul , ROOT, Brian , SAUL, James, D.
IPC分类号： C12Q1/68
摘要： liquid extraction methods are used to recover nucleic acid samples in a fully-automated microfiuidic chip tor biological analysis. A sample is introduced to a rnicrofiuidic chip through a sample acceptor that can be isolated from the surroundings, but. fluidly coupled to a first domain containing a pre-loaded liquid extraction solution. The liquid extraction solution uses one or more thermostable proteinases to extract DNA from the biological sample by submerging an introduced sample and returning with an extracted DNA solution to the first domain, A fraction of the resultant extracted DNA solution is directed through a side channel toward the second domain, where it mixes with PGR reagents and a fraction of pre-PCR solution is thermally cycled in a PGR reaction chamber. The amplified DMA solution is then mixed with a separations solution for separation by capillary electrophoresis and detection by a DNA Analyzer, After the first extraction step, the sample solution is diluted at every processing step.

3. 发明申请

WO2014025500A2 THERMOSTABLE ENZYME-BASED EXTRACTIONS ON AN INTEGRATED MICROFLUIDIC CHIP FOR BIOLOGICAL ANALYSIS 审中-公开
标题翻译：用于生物分析的一体化微流控芯片的基于酶的基于酶的提取
公开(公告)号：WO2014025500A2
公开(公告)日：2014-02-13
申请号：PCT/US2013050628
申请日：2013-07-16
申请人： MICROLAB HORIZON LLC , LANDERS JAMES P , KINNON PAUL , ROOT BRIAN , SAUL JAMES D
发明人： LANDERS JAMES P , KINNON PAUL , ROOT BRIAN , SAUL JAMES D
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/0415 , C12Q2565/629
摘要： liquid extraction methods are used to recover nucleic acid samples in a fully-automated microfiuidic chip tor biological analysis. A sample is introduced to a rnicrofiuidic chip through a sample acceptor that can be isolated from the surroundings, but. fluidly coupled to a first domain containing a pre-loaded liquid extraction solution. The liquid extraction solution uses one or more thermostable proteinases to extract DNA from the biological sample by submerging an introduced sample and returning with an extracted DNA solution to the first domain, A fraction of the resultant extracted DNA solution is directed through a side channel toward the second domain, where it mixes with PGR reagents and a fraction of pre-PCR solution is thermally cycled in a PGR reaction chamber. The amplified DMA solution is then mixed with a separations solution for separation by capillary electrophoresis and detection by a DNA Analyzer, After the first extraction step, the sample solution is diluted at every processing step.
摘要翻译：液体提取方法用于在全自动微流控芯片中生物分析中回收核酸样品。样品通过可以与周围环境隔离的样品受体引入微流控芯片，但是。流体耦合到含有预加载液体提取溶液的第一结构域。液体提取溶液使用一种或多种热稳定蛋白酶，通过浸入引入的样品并将提取的DNA溶液返回到第一结构域，从生物样品中提取DNA。所得提取的DNA溶液的一部分通过侧向通道朝向第二结构域，其与PGR试剂混合，并将一部分预PCR溶液在PGR反应室中热循环。然后将扩增的DMA溶液与分离溶液混合以通过毛细管电泳分离，并通过DNA分析仪进行检测。在第一提取步骤之后，在每个处理步骤中稀释样品溶液。

4. 发明申请

WO2013126714A2 MICROFLUIDIC CARTRIDGE 审中-公开
公开(公告)号：WO2013126714A2
公开(公告)日：2013-08-29
申请号：PCT/US2013/027341
申请日：2013-02-22
申请人： EGAN, Michael , ROOT, Brian , SCOTT, Orion, N. , SOUTH, Douglas, J. , BIENVENUE, Joan , KINNON, Paul , LANDERS, James , SAUL, David , TSUEI, An-Chi , HAYES, Jason , SPRINGER, Matthew , SOLOMON, Matthew , VAN RUIJVEN, Peter
发明人： EGAN, Michael , ROOT, Brian , SCOTT, Orion, N. , SOUTH, Douglas, J. , BIENVENUE, Joan , KINNON, Paul , LANDERS, James , SAUL, David , TSUEI, An-Chi , HAYES, Jason , SPRINGER, Matthew , SOLOMON, Matthew , VAN RUIJVEN, Peter
IPC分类号： B01L3/00
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502784 , B01L3/5029 , B01L7/52 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/0689 , B01L2200/10 , B01L2200/148 , B01L2200/16 , B01L2300/044 , B01L2300/0654 , B01L2300/0663 , B01L2300/0672 , B01L2300/0816 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/14 , B01L2300/161 , B01L2300/18 , B01L2300/1872 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , B01L2400/088 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , G01N21/75
摘要： A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.
摘要翻译：微流体盒可以包括至少一个核酸分析部分。每个核酸分析部分可以包括被配置用于微升体积或更小的流体网络，在流体网络的开始处的样本输入，流体网络中的多个排气口和流体通道，其被配置为实现在流体网络内的流体动力学运动流体网络，流体网络中的提取混合物容器，流体网络中的混合室，流体网络中的放大室和流体网络中的分离通道。核酸分析仪能够使用微流体盒进行核酸分析。核酸分析方法可以使用微流体筒进行。

5. 发明申请

WO2013126714A3 MICROFLUIDIC CARTRIDGE 审中-公开
标题翻译：微型墨盒
公开(公告)号：WO2013126714A3
公开(公告)日：2013-11-14
申请号：PCT/US2013027341
申请日：2013-02-22
申请人： EGAN MICHAEL , ROOT BRIAN , SCOTT ORION N , SOUTH DOUGLAS J , BIENVENUE JOAN , KINNON PAUL , LANDERS JAMES , SAUL DAVID , TSUEI AN-CHI , HAYES JASON , SPRINGER MATTHEW , SOLOMON MATTHEW , VAN RUIJVEN PETER
发明人： EGAN MICHAEL , ROOT BRIAN , SCOTT ORION N , SOUTH DOUGLAS J , BIENVENUE JOAN , KINNON PAUL , LANDERS JAMES , SAUL DAVID , TSUEI AN-CHI , HAYES JASON , SPRINGER MATTHEW , SOLOMON MATTHEW , VAN RUIJVEN PETER
IPC分类号： B01L3/00
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/502784 , B01L3/5029 , B01L7/52 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/0689 , B01L2200/10 , B01L2200/148 , B01L2200/16 , B01L2300/044 , B01L2300/0654 , B01L2300/0663 , B01L2300/0672 , B01L2300/0816 , B01L2300/0832 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/14 , B01L2300/161 , B01L2300/18 , B01L2300/1872 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , B01L2400/088 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , G01N21/75
摘要： A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.
摘要翻译：微流控盒可以包括至少一个核酸分析部分。每个核酸分析部分可以包括被配置用于微升体积或更小体积的流体网络，流体网络开始处的样品输入，流体网络中的多个通气端口和流体通道，其被配置为实现流体网络内的流体动力学运动流体网络中的提取混合物储存器，流体网络中的混合室，流体网络中的扩散室和流体网络中的分离通道。核酸分析仪能够使用微流控盒进行核酸分析。可以使用微流控盒进行核酸分析方法。

6. 发明申请

WO2009148489A1 CHAIR WITH CONTROL SYSTEM 审中-公开
标题翻译：椅子与控制系统
公开(公告)号：WO2009148489A1
公开(公告)日：2009-12-10
申请号：PCT/US2009/002780
申请日：2009-05-05
申请人： STEELCASE INC. , HOLDREDGE, Russell , NORMAN, Christopher , HEIDMANN, Kurt , ROOT, Brian, H. , DINNEWITH, Mark
发明人： HOLDREDGE, Russell , NORMAN, Christopher , HEIDMANN, Kurt , ROOT, Brian, H. , DINNEWITH, Mark
IPC分类号： A47C3/026 , A47C1/032
CPC分类号： A47C1/032
摘要： According to the present invention, a control system (12) for a chair is provided. The control system is comprised of first and second resilient blocks (24,26), first and second stabilizing members (20,22) and first and second rockers (28,30). The first stabilizing member has first and second ends and defines a first horizontal axis. The first end of the first stabilizing member extends into the first resilient block and the second end of the first stabilizing member extends into the second resilient block. The second stabilizing member is coupled to the first stabilizing member by a connector (27). The second stabilizing member has first and second ends and defines a second horizontal axis disposed in parallel to the first horizontal axis. The first end of the second stabilizing member is operably coupled to the first rocker, and the second end of the second stabilizing member is operably coupled to the second rocker.
摘要翻译：根据本发明，提供了一种用于椅子的控制系统（12）。控制系统包括第一和第二弹性块（24,26），第一和第二稳定构件（20,22）以及第一和第二摇臂（28,30）。第一稳定构件具有第一和第二端并且限定第一水平轴线。第一稳定构件的第一端延伸到第一弹性块中，并且第一稳定构件的第二端延伸到第二弹性块中。第二稳定构件通过连接器（27）联接到第一稳定构件。第二稳定构件具有第一和第二端并且限定平行于第一水平轴设置的第二水平轴线。第二稳定构件的第一端可操作地联接到第一摇臂，并且第二稳定构件的第二端可操作地联接到第二摇臂。

7. 发明申请

WO2013169915A1 SELF-COATING SIEVING COPOLYMERS AND PLASTIC DEVICES FOR NUCLEIC ACID SEPARATIONS ON SUCH DEVICES 审中-公开
标题翻译：用于在这些装置上进行核酸分离的自涂层共聚物和塑料装置
公开(公告)号：WO2013169915A1
公开(公告)日：2013-11-14
申请号：PCT/US2013/040165
申请日：2013-05-08
申请人： ROOT, Brian
发明人： ROOT, Brian
IPC分类号： G01N27/447
CPC分类号： G01N27/44747
摘要： The present invention relates to a sieve device and method of making a sieve device comprising a plastic substrate with microchannels extending through the plastic substrate, wherein the microchannels are filled with a composition comprising one or more copolymers. The present invention further relates to a method of separating a mixture of nucleic acid molecules, where the method comprises loading a sample comprising a mixture of nucleic acid molecules onto or into the microchannels of the sieve device of the invention, and separating the mixture of nucleic acid molecules in the sample under conditions effective to achieve single base pair resolution of the nucleic acid molecules.
摘要翻译：本发明涉及一种筛装置及其制造方法，所述筛装置包括具有延伸穿过所述塑料基板的微通道的塑料基板，其中所述微通道填充有包含一种或多种共聚物的组合物。本发明还涉及分离核酸分子的混合物的方法，其中所述方法包括将包含核酸分子的混合物的样品载载到本发明的筛装置的微通道上或分离入核酸的混合物样品中的酸性分子在有效实现单碱基对解离核酸分子的条件下。

8. 发明申请

WO2022060548A1 METHOD AND SYSTEM FOR SAMPLE COLLECTION, STORAGE, PREPARATION AND DETECTION 审中-公开
公开(公告)号：WO2022060548A1
公开(公告)日：2022-03-24
申请号：PCT/US2021/047636
申请日：2021-08-26
申请人： MICROGEM INTERNATIONAL PLC , SCOTT, Orion , BIRCH, Christopher , MILLS, Daniel , SAUL, David , VIGIL, David , GUY, Andrew , WADA, Stan , DE GORORDO, Betina , DODMAN, Stewart , MORAN, Tom , ROOT, Brian , LANDERS, James , YEUNG, Matthew , LI, Jingyi , KNOWLES, Stuart , DIAS, Fernando , GARDNER, Rick
发明人： SCOTT, Orion , BIRCH, Christopher , MILLS, Daniel , SAUL, David , VIGIL, David , GUY, Andrew , WADA, Stan , DE GORORDO, Betina , DODMAN, Stewart , MORAN, Tom , ROOT, Brian , LANDERS, James , YEUNG, Matthew , LI, Jingyi , KNOWLES, Stuart , DIAS, Fernando , GARDNER, Rick
IPC分类号： B01L3/00 , B01L7/00 , C12Q1/68 , C12M1/00
摘要： A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

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