基本信息:
- 专利标题： DEVICES AND METHODS FOR EXTRACTION, SEPARATION AND THERMOCYCLING
- 专利标题（中）：用于提取，分离和热循环的装置和方法
- 申请号：PCT/US2016/056906 申请日：2016-10-13
- 公开(公告)号：WO2017066485A1 公开(公告)日：2017-04-20
- 发明人： LANDERS, James P. , DUVALL, Jacquelyn , LE ROUX, Delphine , ROOT, Brian , MILLS, Daniel , NELSON, Daniel A. , TSUEI, An-Chi , THOMPSON, Brandon L. , LI, Jingyi , BIRCH, Christopher
- 申请人： LANDERS, James P. , DUVALL, Jacquelyn , LE ROUX, Delphine , ROOT, Brian , MILLS, Daniel , NELSON, Daniel A. , TSUEI, An-Chi , THOMPSON, Brandon L. , LI, Jingyi , BIRCH, Christopher
- 申请人地址： 633 Nettle Court Charlottesville, Virginia 22903 US
- 专利权人： LANDERS, James P.,DUVALL, Jacquelyn,LE ROUX, Delphine,ROOT, Brian,MILLS, Daniel,NELSON, Daniel A.,TSUEI, An-Chi,THOMPSON, Brandon L.,LI, Jingyi,BIRCH, Christopher
- 当前专利权人： LANDERS, James P.,DUVALL, Jacquelyn,LE ROUX, Delphine,ROOT, Brian,MILLS, Daniel,NELSON, Daniel A.,TSUEI, An-Chi,THOMPSON, Brandon L.,LI, Jingyi,BIRCH, Christopher
- 当前专利权人地址： 633 Nettle Court Charlottesville, Virginia 22903 US
- 代理机构： SCHEER, Bradley W., Reg. No. 47,059 et al.
- 优先权： US62/241,043 20151013; US62/241,049 20151013; US62/241,055 20151013
- 主分类号： B01L7/00
- IPC分类号： B01L7/00
摘要：
A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.
摘要（中）：
在具有多个腔室和通道的微流体装置中提取，扩增和分离核酸的方法可包括a）将具有核酸的细胞引入微流体装置的第一腔室并使细胞 在第一个室中进行裂解细胞的条件。 该方法可以进一步包括：b）使第一室受到离心力，由此允许具有核酸的裂解物或其一部分通过微流体装置中的第一通道分配到第二室。 该方法还可以包括c）将裂解物或其部分与用于扩增核酸的试剂组合，由此提供第二混合物。 该方法还可以包括：d）使第二室受到离心力，从而使气体从第二混合物排出。
IPC结构图谱:
| B | 作业；运输 |
| --B01 | 一般的物理或化学的方法或装置 |
| ----B01L | 通用化学或物理实验室设备 |
| ------B01L7/00 | 加热或冷却设备 |