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2. 发明申请

WO2014025500A2 THERMOSTABLE ENZYME-BASED EXTRACTIONS ON AN INTEGRATED MICROFLUIDIC CHIP FOR BIOLOGICAL ANALYSIS 审中-公开
标题翻译：用于生物分析的一体化微流控芯片的基于酶的基于酶的提取
公开(公告)号：WO2014025500A2
公开(公告)日：2014-02-13
申请号：PCT/US2013050628
申请日：2013-07-16
申请人： MICROLAB HORIZON LLC , LANDERS JAMES P , KINNON PAUL , ROOT BRIAN , SAUL JAMES D
发明人： LANDERS JAMES P , KINNON PAUL , ROOT BRIAN , SAUL JAMES D
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/0415 , C12Q2565/629
摘要： liquid extraction methods are used to recover nucleic acid samples in a fully-automated microfiuidic chip tor biological analysis. A sample is introduced to a rnicrofiuidic chip through a sample acceptor that can be isolated from the surroundings, but. fluidly coupled to a first domain containing a pre-loaded liquid extraction solution. The liquid extraction solution uses one or more thermostable proteinases to extract DNA from the biological sample by submerging an introduced sample and returning with an extracted DNA solution to the first domain, A fraction of the resultant extracted DNA solution is directed through a side channel toward the second domain, where it mixes with PGR reagents and a fraction of pre-PCR solution is thermally cycled in a PGR reaction chamber. The amplified DMA solution is then mixed with a separations solution for separation by capillary electrophoresis and detection by a DNA Analyzer, After the first extraction step, the sample solution is diluted at every processing step.
摘要翻译：液体提取方法用于在全自动微流控芯片中生物分析中回收核酸样品。样品通过可以与周围环境隔离的样品受体引入微流控芯片，但是。流体耦合到含有预加载液体提取溶液的第一结构域。液体提取溶液使用一种或多种热稳定蛋白酶，通过浸入引入的样品并将提取的DNA溶液返回到第一结构域，从生物样品中提取DNA。所得提取的DNA溶液的一部分通过侧向通道朝向第二结构域，其与PGR试剂混合，并将一部分预PCR溶液在PGR反应室中热循环。然后将扩增的DMA溶液与分离溶液混合以通过毛细管电泳分离，并通过DNA分析仪进行检测。在第一提取步骤之后，在每个处理步骤中稀释样品溶液。

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