专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007081664A3 USE OF ACID SCAVENGERS FOR THE SYNTHESIS OF STANDARD LENGTH AND LONG-MER NUCLEIC ACID ARRAYS 审中-公开
标题翻译：酸性清除剂在合成标准长度和长链核酸阵列中的应用
公开(公告)号：WO2007081664A3
公开(公告)日：2007-11-22
申请号：PCT/US2006062669
申请日：2006-12-28
申请人： AFFYMETRIX INC , KUIMELIS ROBERT G , MCGALL GLENN H , GOLDBERG MARTIN J , XU GUANGYU
发明人： KUIMELIS ROBERT G , MCGALL GLENN H , GOLDBERG MARTIN J , XU GUANGYU
IPC分类号： B01J19/00 , C07H21/00 , C40B50/14
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagent are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：采用可用可活化去保护试剂裂解的保护基团来实现各种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。在本发明的优选实施方案中，可活化的脱保护试剂是光酸产生剂，保护基团是核酸的DMT和氨基酸的tBOC。本发明具有广泛的应用范围，特别适用于聚合物的固相组合合成。

2. 发明申请

WO0006771A2 NUCLEIC ACID LABELING COMPOUNDS 审中-公开
标题翻译：核酸标签化合物
公开(公告)号：WO0006771A2
公开(公告)日：2000-02-10
申请号：PCT/US9912390
申请日：1999-07-20
申请人： AFFYMETRIX INC , MCGALL GLENN H , BARONE ANTHONY D
发明人： MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： G01N33/50 , C07F9/6558 , C07F9/6561 , C07H19/04 , C07H19/052 , C07H19/06 , C07H19/10 , C07H19/12 , C07H19/23 , C07H21/00 , C07H21/04 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N21/78 , G01N33/53 , G01N33/566
CPC分类号： C07H19/052 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/12 , C07H21/00
摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.
摘要翻译：公开了含有杂环衍生物的核酸标记化合物。通过将杂环衍生物与环状基团（例如呋喃核糖衍生物）缩合来合成含杂环衍生物的化合物。标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

3. 发明申请

WO1998020967A1 CHEMICAL AMPLIFICATION FOR THE SYNTHESIS OF PATTERNED ARRAYS 审中-公开
标题翻译：用于合成图案阵列的化学放大
公开(公告)号：WO1998020967A1
公开(公告)日：1998-05-22
申请号：PCT/US1997021209
申请日：1997-11-13
申请人： AFFYMETRIX, INC. , BEECHER, Jody, E. , GOLDBERG, Martin, J. , MCGALL, Glenn, H.
发明人： AFFYMETRIX, INC.
IPC分类号： B01J19/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00427 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , C07B61/00 , C07B2200/11 , C07H21/00 , C07H21/04 , C07K1/04 , C07K1/045 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B60/14 , Y02P20/55
摘要： Radiation-activated catalyst (RACs), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RACs produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least one product that removes protecting groups from synthesis intermediates. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：使用辐射活化催化剂（RAC），自催化反应和保护基团来实现多种聚合物图案阵列的高灵敏度，高分辨率的辐射导向组合合成。当照射时，RAC产生可与增强剂反应的催化剂，例如参与自催化反应的那些。自催化反应产生至少一种从合成中间体中除去保护基的产物。本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。

4. 发明申请

WO0220150A3 PHOTOCLEAVABLE PROTECTING GROUPS 审中-公开
标题翻译：光电保护组
公开(公告)号：WO0220150A3
公开(公告)日：2003-03-13
申请号：PCT/IB0101650
申请日：2001-09-11
申请人： AFFYMETRIX INC , MCGALL GLENN H , BARONE ANTHONY D
发明人： MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K1/04 , C07K1/06 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C07K17/06
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K1/04 , C07K1/047 , C07K1/062 , C07K1/063 , C07K1/064 , C07K1/065 , C07K1/066 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , Y02P20/55
摘要： Novel compounds are provided which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. The compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting groups has the general formula (Y), wherein: (Y) is a chemical structure as shown the Figure. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
摘要翻译：提供了新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。化合物通常是光不稳定的，并且包含可以通过光解除去以揭示反应性基团的保护基团。保护基具有通式（Y），其中：（Y）是如图所示的化学结构。还提供了使用上述保护的化合物从成分分子在载体上形成多种化合物的方法，每种化合物占据支持体的单独的预定区域。

5. 发明申请

WO2000006771A3 NUCLEIC ACID LABELING COMPOUNDS 审中-公开
公开(公告)号：WO2000006771A3
公开(公告)日：2000-02-10
申请号：PCT/US1999/012390
申请日：1999-07-20
申请人： AFFYMETRIX, INC. , MCGALL, Glenn, H. , BARONE, Anthony, D.
发明人： MCGALL, Glenn, H. , BARONE, Anthony, D.
IPC分类号： C07H19/04
摘要： Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

6. 发明申请

WO1997027317A1 NUCLEIC ACID ANALYSIS TECHNIQUES 审中-公开
标题翻译：核酸分析技术
公开(公告)号：WO1997027317A1
公开(公告)日：1997-07-31
申请号：PCT/US1997001603
申请日：1997-01-22
申请人： AFFYMETRIX, INC. , LOCKHART, David, J. , CHEE, Mark , GUNDERSON, Kevin , LAI, Chaoqiang , WODICKA, Lisa , CRONIN, Maureen, T. , LEE, Danny , TRAN, Huu, M. , MATSUZAKI, Hajime , McGALL, Glenn, H. , BARONE, Anthony, D.
发明人： AFFYMETRIX, INC.
IPC分类号： C12Q01/00
CPC分类号： C12Q1/6809 , C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6816 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , C12Q2565/501 , C12Q2521/501
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater then 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译：本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。该方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。本发明还提供了一种终止标记核酸的方法。在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。

7. 发明申请

WO2007081664A2 USE OF ACID SCAVENGERS FOR THE SYNTHESIS OF STANDARD LENGTH AND LONG-MER NUCLEIC ACID ARRAYS 审中-公开
标题翻译：用于标准长度和长度核酸阵列合成的酸性螯合剂的使用
公开(公告)号：WO2007081664A2
公开(公告)日：2007-07-19
申请号：PCT/US2006/062669
申请日：2006-12-28
申请人： AFFYMETRIX, INC. , KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
发明人： KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagent are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：可以用可活化的脱保护试剂切割的保护基团用于实现多种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。在本发明的优选实施方案中，可活化的去保护试剂是光酸产生剂，保护基是核酸的DMT和氨基酸的tBOC。本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。

8. 发明申请

WO2004007751A2 NUCLEIC ACID LABELING METHODS 审中-公开
标题翻译：核酸标记方法
公开(公告)号：WO2004007751A2
公开(公告)日：2004-01-22
申请号：PCT/US0322035
申请日：2003-07-14
申请人： AFFYMETRIX INC , COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
发明人： COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68 , C12Q
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20 , C12Q1/6816 , C12Q2565/501 , C12Q2563/107 , C12Q2521/501
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3'-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3'-AppN-3'-linker-biotin is used as donor molecule. In another aspect of the invention, a method is provided for analyzing a nucleic acid population on a nucleic acid microarray comprising providing a nucleic acid population or converting the nucleic acid population into nucleic acid fragments; ligating the nucleic acid population or fragments to a labeled nucleic acid molecule to form labeled nucleic acid population or fragments using a ligase; hybridizing the labeled nucleic acid population or fragments to an array of nucleic acid probes, and determining hybridization signals of the probes as an indication of levels of the nucleic acids in the nucleic acid population.
摘要翻译：在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化的RNA）的方法。在一个实施方案中，T4 RNA连接酶用于将3'-生物素化的AMP或CMP供体附着至RNA受体分子。在另一个实施方案中，使用焦磷酸分子3'-AppN-3'-连接体 - 生物素作为供体分子。在本发明的另一方面，提供了用于分析核酸微阵列上的核酸群体的方法，其包括提供核酸群体或将核酸群体转换成核酸片段; 使用连接酶将核酸群或片段连接至标记的核酸分子以形成标记的核酸群或片段; 将标记的核酸群体或片段与核酸探针阵列杂交，并确定探针的杂交信号，作为核酸群体中核酸水平的指示。

9. 发明申请

WO2004001506A2 ANTIREFLECTIVE COATINGS FOR HIGH-RESOLUTION PHOTOLITHOGRAPHIC SYNTHESIS OF DNA ARRAY 审中-公开
标题翻译： DNA阵列高分辨光谱合成的抗反射涂层
公开(公告)号：WO2004001506A2
公开(公告)日：2003-12-31
申请号：PCT/US2003/019586
申请日：2003-06-20
申请人： AFFYMETRIX, INC. , TRULSON, Mark , MCGALL, Glenn H. , SYWE, Bei-Shen , KAJISA, Lisa T. , TRUONG, Dana
发明人： TRULSON, Mark , MCGALL, Glenn H. , SYWE, Bei-Shen , KAJISA, Lisa T. , TRUONG, Dana
IPC分类号： G03F
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00711 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , G03F7/091 , C12Q2545/101
摘要： The present inventions provides an array of polymers and method of forming arrays of polymers by providing a substrate having a first layer including one or more dielectric coatings on a solid support and a second layer including a plurality of polymers disposed on the first layer. The invention also provides methods for forming an array of polymers on a substrate using light-directed synthesis by providing a substrate having a first layer including one or more dielectric coating on a solid support; derivatizing the first layer by contacting the first layer with a silanation reagent, and a second layer disposed on said first layer wherein the second layer includes functional groups protected with a photolabile protecting group.
摘要翻译：本发明提供了一种聚合物阵列和通过提供一种具有包含在固体载体上的一个或多个电介质涂层的第一层的基底和包含设置在第一层上的多个聚合物的第二层的基底来形成聚合物阵列的方法。本发明还提供了使用光导合成在衬底上形成聚合物阵列的方法，该衬底具有在固体支持体上具有一个或多个介电涂层的第一层; 通过使第一层与硅烷化试剂接触来衍生第一层，以及设置在所述第一层上的第二层，其中第二层包括用光不稳定保护基团保护的官能团。

10. 发明申请

WO2003087166A2 PHOTOACTIVATABLE SILANE COMPOUNDS AND METHODS FOR THEIR SYNTHESIS AND USE 审中-公开
标题翻译：可光化的硅烷化合物及其合成和使用方法
公开(公告)号：WO2003087166A2
公开(公告)日：2003-10-23
申请号：PCT/US2003/010528
申请日：2003-04-07
申请人： AFFYMETRIX, INC. , LI, Handong , MCGALL, Glenn, H.
发明人： LI, Handong , MCGALL, Glenn, H.
IPC分类号： C08F
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00378 , B01J2219/00608 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C40B50/18 , C40B60/14 , G03F7/165 , Y10T428/31663
摘要： In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.
摘要翻译：在本发明的一个方面，提供了可光活化的硅烷化合物及其合成和使用的方法。在一个实施方案中，合成的可光活化硅烷化合物由下式表示：PG-LS-SN，其中PG是可光活化基团，LS是连接基和间隔基，SN是硅烷基。硅烷使可光活化的硅烷化合物共价结合到诸如二氧化硅的底物表面。可光活化基团形成疏水层，其可以与一层亲水性官能聚合物光化学交联。在另一个实施方案中，公开了合成疏水层的底物和亲水性功能聚合物层之后的玻璃表面的方法。然后使用光刻和生物聚合物合成将一系列生物聚合物如核酸和肽共价连接到基底上。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式