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1. 发明申请

WO2013163263A3 METHODS FOR SORTING NUCLEIC ACIDS AND MULTIPLEXED PREPARATIVE IN VITRO CLONING 审中-公开
标题翻译：分离核酸和复制准备在体外克隆中的方法
公开(公告)号：WO2013163263A3
公开(公告)日：2014-03-06
申请号：PCT/US2013037921
申请日：2013-04-24
申请人： GEN9 INC , JACOBSON JOSEPH , GOLDBERG MARTIN J , KUNG LI-YUN A , SCHINDLER DANIEL , HUDSON MICHAEL E
发明人： JACOBSON JOSEPH , GOLDBERG MARTIN J , KUNG LI-YUN A , SCHINDLER DANIEL , HUDSON MICHAEL E
IPC分类号： C12Q1/68 , C12N15/10 , C12P19/34
CPC分类号： C12N15/1065 , C12N15/1034 , C12N15/1093
摘要： Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.
摘要翻译：方法和组合物涉及使用高通量测序分选和克隆高保真度核酸。具体地，具有所需预定序列的核酸分子可以从包含多个具有正确和不正确序列的核酸的库中分选。

2. 发明申请

WO2013163263A2 METHODS FOR SORTING NUCLEIC ACIDS AND MULTIPLEXED PREPARATIVE IN VITRO CLONING 审中-公开
标题翻译：分离核酸和复制准备在体外克隆中的方法
公开(公告)号：WO2013163263A2
公开(公告)日：2013-10-31
申请号：PCT/US2013/037921
申请日：2013-04-24
申请人： GEN9, INC. , JACOBSON, Joseph , GOLDBERG, Martin J. , KUNG, Li-Yun A. , SCHINDLER, Daniel , HUDSON, Michael E.
发明人： JACOBSON, Joseph , GOLDBERG, Martin J. , KUNG, Li-Yun A. , SCHINDLER, Daniel , HUDSON, Michael E.
IPC分类号： C12N15/10
CPC分类号： C12N15/1065 , C12N15/1034 , C12N15/1093
摘要： Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.
摘要翻译：方法和组合物涉及使用高通量测序分选和克隆高保真度核酸。具体地，具有所需预定序列的核酸分子可以从包含多个具有正确和不正确序列的核酸的库中分选。

3. 发明申请

WO2007081664A3 USE OF ACID SCAVENGERS FOR THE SYNTHESIS OF STANDARD LENGTH AND LONG-MER NUCLEIC ACID ARRAYS 审中-公开
标题翻译：酸性清除剂在合成标准长度和长链核酸阵列中的应用
公开(公告)号：WO2007081664A3
公开(公告)日：2007-11-22
申请号：PCT/US2006062669
申请日：2006-12-28
申请人： AFFYMETRIX INC , KUIMELIS ROBERT G , MCGALL GLENN H , GOLDBERG MARTIN J , XU GUANGYU
发明人： KUIMELIS ROBERT G , MCGALL GLENN H , GOLDBERG MARTIN J , XU GUANGYU
IPC分类号： B01J19/00 , C07H21/00 , C40B50/14
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagent are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：采用可用可活化去保护试剂裂解的保护基团来实现各种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。在本发明的优选实施方案中，可活化的脱保护试剂是光酸产生剂，保护基团是核酸的DMT和氨基酸的tBOC。本发明具有广泛的应用范围，特别适用于聚合物的固相组合合成。

4. 发明申请

WO1998020967A1 CHEMICAL AMPLIFICATION FOR THE SYNTHESIS OF PATTERNED ARRAYS 审中-公开
标题翻译：用于合成图案阵列的化学放大
公开(公告)号：WO1998020967A1
公开(公告)日：1998-05-22
申请号：PCT/US1997021209
申请日：1997-11-13
申请人： AFFYMETRIX, INC. , BEECHER, Jody, E. , GOLDBERG, Martin, J. , MCGALL, Glenn, H.
发明人： AFFYMETRIX, INC.
IPC分类号： B01J19/00
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00427 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , C07B61/00 , C07B2200/11 , C07H21/00 , C07H21/04 , C07K1/04 , C07K1/045 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B60/14 , Y02P20/55
摘要： Radiation-activated catalyst (RACs), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RACs produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least one product that removes protecting groups from synthesis intermediates. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：使用辐射活化催化剂（RAC），自催化反应和保护基团来实现多种聚合物图案阵列的高灵敏度，高分辨率的辐射导向组合合成。当照射时，RAC产生可与增强剂反应的催化剂，例如参与自催化反应的那些。自催化反应产生至少一种从合成中间体中除去保护基的产物。本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。

5. 发明申请

WO2007081664A2 USE OF ACID SCAVENGERS FOR THE SYNTHESIS OF STANDARD LENGTH AND LONG-MER NUCLEIC ACID ARRAYS 审中-公开
标题翻译：用于标准长度和长度核酸阵列合成的酸性螯合剂的使用
公开(公告)号：WO2007081664A2
公开(公告)日：2007-07-19
申请号：PCT/US2006/062669
申请日：2006-12-28
申请人： AFFYMETRIX, INC. , KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
发明人： KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagent are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：可以用可活化的脱保护试剂切割的保护基团用于实现多种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。在本发明的优选实施方案中，可活化的去保护试剂是光酸产生剂，保护基是核酸的DMT和氨基酸的tBOC。本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。

6. 发明申请

WO0018962A9 METHODS AND COMPOSITIONS FOR AMPLIFYING DETECTABLE SIGNALS IN SPECIFIC BINDING ASSAYS 审中-公开
标题翻译：用于在特定结合测定中扩增可检测信号的方法和组合物
公开(公告)号：WO0018962A9
公开(公告)日：2000-08-24
申请号：PCT/US9922584
申请日：1999-09-28
申请人： AFFYMETRIX INC , GOLDBERG MARTIN J , YELAGALAWADI GOVINDA RAO S , TANIMOTO EUGENE YUJI , TRAN HUU MINH , DONG HELIN , LOCKHART DAVID J , RYDER THOMAS B , WARRINGTON JANET A , BEECHER JODY
发明人： GOLDBERG MARTIN J , YELAGALAWADI GOVINDA RAO S , TANIMOTO EUGENE YUJI , TRAN HUU MINH , DONG HELIN , LOCKHART DAVID J , RYDER THOMAS B , WARRINGTON JANET A , BEECHER JODY
IPC分类号： C12N15/09 , C12Q1/68 , G01N37/00 , G01N33/58
CPC分类号： C12Q1/682 , C12Q1/6804 , Y10S436/807 , C12Q2565/501 , C12Q2563/131 , C12Q2527/125 , C12Q2563/107 , C12Q2563/125 , C12Q2563/143 , C12Q2537/125
摘要： Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hybridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of large number of nucleic acids.
摘要翻译：提供了使用特异性结合测定法检测样品中的靶分子的方法和化合物。具体而言，提供了用于检测样品中的核酸靶标的方法。在一个实施方案中，所述方法包括使包含靶核酸序列的核酸靶与包含探针核酸序列的核酸探针杂交，其中所述靶包含结合配体。使杂交的靶与包含能够结合结合配体的多个位点的受体接触以将受体与结合配体复合，并使受体与包含多个结合配体的扩增试剂接触以将扩增试剂复合成受体。然后检测复合的扩增试剂的存在，例如通过检测例如受体或扩增试剂上的可检测标记（例如荧光标记）的存在。任选地，使包含多个结合配体的扩增试剂与标记的受体分子接触，从而将多个标记的受体分子与扩增试剂复合，并检测与扩增试剂复合的标记的受体分子。这允许可检测信号被增强和放大。在一个实施方案中，结合配体是生物素，受体是链霉抗生物素蛋白，扩增试剂是抗体或DNA基质。在另一个实施方案中，固定在表面上的不同核酸探针的阵列，每个核酸探针在表面上都具有限定的序列和位置，可以用于测定中，从而允许筛选和检测大量核酸的结合。

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