专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2019079458A1 DETECTION DEVICE AND METHOD 审中-公开
公开(公告)号：WO2019079458A1
公开(公告)日：2019-04-25
申请号：PCT/US2018/056297
申请日：2018-10-17
申请人： AFFYMETRIX, INC.
发明人： KAMNEVA, Olga , GOLLUB, Jeremy , VARMA, Ram
IPC分类号： G16B25/10 , G16B40/20
CPC分类号： G16B40/00 , G16B25/00
摘要： A system and method utilizes multi-sample batch controls for high throughput copy number calling in a small number of fixed regions where copy number changes are expected. The system and method utilize intermediate copy numbers applied to regions mapped with density-based clustering and prior knowledge to make final copy number calls for components.

2. 发明申请

WO2018223057A8 ARRAY-BASED METHODS FOR ANALYSING MIXED SAMPLES USING DIFFERENT ALLELE-SPECIFIC LABELS, IN PARTICULAR FOR DETECTION OF FETAL ANEUPLOIDIES 审中-公开
公开(公告)号：WO2018223057A8
公开(公告)日：2018-12-06
申请号：PCT/US2018/035688
申请日：2018-06-01
申请人： AFFYMETRIX, INC.
发明人： SCHMIDT, Jeanette , MIZRAHI MAN, Orna , LI, Jiang
IPC分类号： C12Q1/6827 , C12Q1/6837
摘要： Provided includes methods and systems useful in array-based analysis of mixed nucleic acid populations, including for genotyping and copy number analysis of the various subpopulations of the mixed nucleic acid population. Also provided includes methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken from an organism.

3. 发明申请

WO2018223055A1 ARRAY-BASED METHODS FOR ANALYSING MIXED SAMPLES USING DIFFERENTLY LABELLED ALLELE-SPECIFIC PROBES 审中-公开
公开(公告)号：WO2018223055A1
公开(公告)日：2018-12-06
申请号：PCT/US2018/035686
申请日：2018-06-01
申请人： AFFYMETRIX, INC.
发明人： SAPOLSKY, Ronald , SHAPERO, Michael , SCHMIDT, Jeanette , FUNG, Eric , MIZRAHI MAN, Orna , LI, Jiang , CHADHA, Monica , SHUKLA, Anju
IPC分类号： C12Q1/6827 , C12Q1/6837
摘要： This disclosure provides methods and systems useful in array-based analysis of mixed nucleic acid populations, including for multiplex genotyping of a mixed nucleic acid sample and for detecting differences in copy number of a target polynucleotide and/or a target chromosome (e.g., microdeletions, duplications and aneuploidies). The disclosure also provides methods and systems useful in the diagnosis of genetic abnormalities in a mixed nucleic acid population taken non-invasively from an organism, such as a sample of blood, plasma, serum, urine stool or saliva. The disclosed methods and systems find use in multiple applications, including prenatal testing and cancer diagnostics. The disclosure is based on the hybridisation of amplified fragments from the sample, e.g. a maternal sample, which may employ molecular inversion probes MIP to an oligonucleotidfe array and the detection of the alleles based on different signals from the different alleles of the SNP. The disclosure also discloses how the determination of the allele ratio may be used in the determination of fetal and maternal CNVs, e.g. aneuploidies.

4. 发明申请

WO2017044993A2 NUCLEIC ACID ANALYSIS BY JOINING BARCODED POLYNUCLEOTIDE PROBES 审中-公开
标题翻译：核糖核酸分析通过加入骨架多核苷酸探针
公开(公告)号：WO2017044993A2
公开(公告)日：2017-03-16
申请号：PCT/US2016/060991
申请日：2016-11-08
申请人： AFFYMETRIX, INC.
发明人： KOSHINSKY, Heather , CURRY, John D. , O'CALLAHAN, Robert , MCCOY, Adam , FITZPATRICK, Daniel , DICKINSON, Philip H. , SCHWEITZER, Anthony C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q2525/155 , C12Q2525/191
摘要： Disclosed are compositions, methods and kits for determining the presence, absence, amount, copy number, or other characteristics of one or more polynucleotide sequences in two or more samples and use thereof in genotyping, evaluation of copy number variation, expression analysis, determination of splice variants and fusion genes, and other genetic analyses.
摘要翻译：公开了用于确定两个或更多个样品中一个或多个多核苷酸序列的存在，不存在，数量，拷贝数或其他特征的组合物，方法和试剂盒，并且在基因分型，拷贝数变异评估，表达分析，剪接变体和融合基因，以及其他遗传分析。

5. 发明申请

WO2015120273A1 DIFFERENTIAL DIAGNOSIS OF HEPATIC NEOPLASMS 审中-公开
标题翻译： HEPATIC NEOPLASMS的差异诊断
公开(公告)号：WO2015120273A1
公开(公告)日：2015-08-13
申请号：PCT/US2015/014820
申请日：2015-02-06
申请人： THE GENERAL HOSPITAL CORPORATION , AFFYMETRIX, INC.
发明人： DESHPANDE, Vikram , GANDHI, Manoj , NGUYEN, Quan , MA, Yunqing , TING, David Tsai , RIVERA, Miguel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6809 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16
摘要： Methods for making a differential diagnosis of hepatic neoplasms, e.g., tumors, and for identifying metastatic tumors of hepatic origin, based on detection of levels of albumin mRNA. The methods can also be used to select treatments or guide treatment decisions.
摘要翻译：基于检测白蛋白mRNA的水平，进行肝肿瘤鉴别诊断的方法，例如肿瘤，以及鉴定肝源性转移性肿瘤。这些方法也可用于选择治疗或指导治疗决定。

6. 发明申请

WO2007081664A2 USE OF ACID SCAVENGERS FOR THE SYNTHESIS OF STANDARD LENGTH AND LONG-MER NUCLEIC ACID ARRAYS 审中-公开
标题翻译：用于标准长度和长度核酸阵列合成的酸性螯合剂的使用
公开(公告)号：WO2007081664A2
公开(公告)日：2007-07-19
申请号：PCT/US2006/062669
申请日：2006-12-28
申请人： AFFYMETRIX, INC. , KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
发明人： KUIMELIS, Robert, G. , MCGALL, Glenn, H. , GOLDBERG, Martin, J. , XU, Guangyu
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagent are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译：可以用可活化的脱保护试剂切割的保护基团用于实现多种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。在本发明的优选实施方案中，可活化的去保护试剂是光酸产生剂，保护基是核酸的DMT和氨基酸的tBOC。本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。

7. 发明申请

WO2004007684A3 SYNTHETIC TAG GENES 审中-公开
标题翻译：合成标签基因
公开(公告)号：WO2004007684A3
公开(公告)日：2005-10-20
申请号：PCT/US0321990
申请日：2003-07-14
申请人： AFFYMETRIX INC , CHRISTIANS FREDERICK C
发明人： CHRISTIANS FREDERICK C
IPC分类号： C07H21/00 , C07H21/04 , C12N20060101 , C12N1/00 , C12N9/22 , C12N15/11 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6837 , C12Q2545/101 , C12Q2525/179
摘要： In one aspect of the invention, a method to construct a synthetic "gene" composed of linked synthetic Tag gene sequences is provided. In one embodiment, the genes, about 500 to 4000 base pairs long, are made by annealing and extending overlapping 60mer oligonucleotides followed by cloning into a plasmid vector. Both poly(A)-tailed sense (Tag) RNA and antisense (Tag Probe) RNA can be produced from the clones by in-vitro transcription. In another embodiment, the genes can be used as exogenous spikes for any sample. In another aspect of the invention, these synthetic gene spikes can serve as normalization controls in gene expression monitoring experiments and can also be used to assess system specificity, sensitivity, and dynamic range. These synthetic Tag genes are thus useful in assay development, in product development and validation, and for quality control.
摘要翻译：在本发明的一个方面，提供了构建由连接的合成Tag基因序列组成的合成“基因”的方法。在一个实施方案中，约500至4000个碱基对长的基因通过退火并延伸重叠的60mer寡核苷酸，然后克隆到质粒载体中制备。聚（A） - 尾（RNA）和反义（Tag Probe）RNA都可以通过体外转录从克隆产生。在另一个实施方案中，所述基因可用作任何样品的外源尖峰。在本发明的另一方面，这些合成基因尖峰可用作基因表达监测实验中的归一化对照，也可用于评估系统特异性，灵敏度和动态范围。因此，这些合成的Tag基因可用于测定开发，产品开发和验证以及质量控制。

8. 发明申请

WO2004044700A3 METHODS, COMPOSITIONS AND COMPUTER SOFTWARE PRODUCTS FOR INTERROGATING SEQUENCE VARIATIONS IN FUNCTIONAL GENOMIC REGIONS 审中-公开
公开(公告)号：WO2004044700A3
公开(公告)日：2004-05-27
申请号：PCT/US2003/035819
申请日：2003-11-12
申请人： AFFYMETRIX, INC. , GINGERAS, Thomas, R.
发明人： GINGERAS, Thomas, R.
IPC分类号： C12Q1/68
摘要： In one aspect of the invention, methods, compositions and computer software products are provided to detect genetic variations in functional regions of a genome. In some embodiments, oligonucleotide tiling probe arrays are used to detect transcribed regions, transcription factor binding regions, etc. Genetic variations in these regions can determined with, e.g., a whole genome sampling assay.

9. 发明申请

WO2004044225A2 METHODS FOR IDENTIFYING DNA COPY NUMBER CHANGES 审中-公开
标题翻译：鉴定DNA复制数变化的方法
公开(公告)号：WO2004044225A2
公开(公告)日：2004-05-27
申请号：PCT/US2003/036018
申请日：2003-11-12
申请人： AFFYMETRIX, INC.
发明人： HUANG, Jing , SHAPERO, Michael, H. , JONES, Keith
IPC分类号： C12Q
CPC分类号： G06F19/20 , C12Q1/6827 , C12Q1/6837 , G06F19/18 , C12Q2545/101
摘要： Methods of identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer.
摘要翻译：公开了鉴定基因组DNA拷贝数变化的方法。公开了鉴定纯合缺失和遗传扩增的方法。还公开了一种设计用于检测多个不同序列的存在或不存在的探针阵列。探针被设计为与预测存在于复杂度降低的样品中的序列杂交。与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。该方法可用于诊断癌症。

10. 发明申请

WO2004007751A2 NUCLEIC ACID LABELING METHODS 审中-公开
标题翻译：核酸标记方法
公开(公告)号：WO2004007751A2
公开(公告)日：2004-01-22
申请号：PCT/US0322035
申请日：2003-07-14
申请人： AFFYMETRIX INC , COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
发明人： COLE KYLE B , TRUONG VIVI , MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/68 , C12Q
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20 , C12Q1/6816 , C12Q2565/501 , C12Q2563/107 , C12Q2521/501
摘要： In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3'-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3'-AppN-3'-linker-biotin is used as donor molecule. In another aspect of the invention, a method is provided for analyzing a nucleic acid population on a nucleic acid microarray comprising providing a nucleic acid population or converting the nucleic acid population into nucleic acid fragments; ligating the nucleic acid population or fragments to a labeled nucleic acid molecule to form labeled nucleic acid population or fragments using a ligase; hybridizing the labeled nucleic acid population or fragments to an array of nucleic acid probes, and determining hybridization signals of the probes as an indication of levels of the nucleic acids in the nucleic acid population.
摘要翻译：在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化的RNA）的方法。在一个实施方案中，T4 RNA连接酶用于将3'-生物素化的AMP或CMP供体附着至RNA受体分子。在另一个实施方案中，使用焦磷酸分子3'-AppN-3'-连接体 - 生物素作为供体分子。在本发明的另一方面，提供了用于分析核酸微阵列上的核酸群体的方法，其包括提供核酸群体或将核酸群体转换成核酸片段; 使用连接酶将核酸群或片段连接至标记的核酸分子以形成标记的核酸群或片段; 将标记的核酸群体或片段与核酸探针阵列杂交，并确定探针的杂交信号，作为核酸群体中核酸水平的指示。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式