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1. 发明申请

WO2009135214A3 RNA POLYPHOSPHATASE COMPOSITIONS, KITS, AND USES THEREOF 审中-公开
标题翻译： RNA多磷酸酯组合物，胶囊及其用途
公开(公告)号：WO2009135214A3
公开(公告)日：2010-03-11
申请号：PCT/US2009042729
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD
IPC分类号： C12N9/14 , C12Q1/42 , C12Q1/68
CPC分类号： C12Q1/42 , C12N9/14 , C12P19/34 , Y10T436/143333
摘要： The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.
摘要翻译：本发明涉及本领域先前未描述的RNA 5'多磷酸酶的发现，所述酶的发现方法，所述酶的组合物，所述酶的制备方法以及使用所述酶进行生物医学研究的各种方法和试剂盒，用于人和非人诊断，用于生产治疗产品和其他应用。具体地，一些实施方案提供了使用RNA多磷酸酶用于使用生物样品或来自体外封端反应的样品分离，纯化，生产和测定加帽RNA的组合物，试剂盒和方法，其中所述样品还含有未被封端的RNA 。其他实施方案提供组合物，试剂盒和方法，其中RNA多磷酸酶包含用于分析物特异性测定的信号放大酶。

2. 发明申请

WO2009135212A2 SELECTIVE 5' LIGATION TAGGING OF RNA 审中-公开
标题翻译：选择性5'RNA结合标记
公开(公告)号：WO2009135212A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042723
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY
IPC分类号： C12N15/10 , C07H21/02 , C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/6855 , C12Q2525/207 , C12Q2525/125 , C12Q2521/525
摘要： The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5' monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5' ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5' ends. The 5'tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.
摘要翻译：本发明提供了使用RNA 5'多磷酸酶，RNA 5'单磷酸酶，加帽酶，脱帽酶，核酸焦磷酸酶和RNA连接酶以及其他酶的新型组合物，试剂盒和方法，用于选择性5'连接标记所需类别的 RNA分子就其5'端的特定化学部分而言不同。 5'标签的RNA分子可用于各种用途的标记的第一站cDNA，双链cDNA和有义或反义RNA的合成。

3. 发明申请

WO2004048596A3 METHODS FOR USING PRIMERS THAT ENCODE ONE STRAND OF A DOUBLE-STRANDED PROMOTER 审中-公开
标题翻译：使用一个双向拉伸推进器的一个条纹的方法
公开(公告)号：WO2004048596A3
公开(公告)日：2004-08-26
申请号：PCT/US0337454
申请日：2003-11-21
申请人： EPICT TECHNOLOGIES , DAHL GARY A , JENDRISAK JEROME J
发明人： DAHL GARY A , JENDRISAK JEROME J
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6853 , C12Q2531/119 , C12Q2521/301 , C12Q2525/125
摘要： The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining circular single-stranded DNA transcription substrates using a promoter primer that encodes one strand of a double-stranded promoter. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, or making RNAi.
摘要翻译：本发明提供使用RNA聚合酶制备对应于靶序列的转录产物的方法，组合物和试剂盒，通过使用编码一条双链启动子的启动子引物获得环状单链DNA转录底物。本发明对于研究，诊断和治疗应用具有广泛的适用性，例如制备对应于mRNA的cDNA，制备有义或反义探针，检测基因或生物体特异性序列或制备RNAi。

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