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1. 发明申请

WO2008085296A1 SEAFOOD SPOILAGE INDICATOR 审中-公开
标题翻译：海鲜指示器
公开(公告)号：WO2008085296A1
公开(公告)日：2008-07-17
申请号：PCT/US2007/025624
申请日：2007-12-14
申请人： AGENTASE, LLC , LEECH, Anna, M. , PALUMBO, Jessica, L. , BERBERICH, Jason, A. , LEJEUNE, Keith, E.
发明人： LEECH, Anna, M. , PALUMBO, Jessica, L. , BERBERICH, Jason, A. , LEJEUNE, Keith, E.
IPC分类号： G01N33/02 , C12M3/00 , C12Q1/28 , C12Q1/26
CPC分类号： G01N33/02
摘要： An enzyme based nondestructive sensor for the qualitative detection of spoilage in seafood is provided wherein the sensor does not alter the physical composition of the seafood specimen. The sensor comprises a sampling matrix, at least three or more enzymes in contact with the sampling matrix, and at least one indicator compound in contact with the sampling matrix. The enzymes are capable of interacting with four target chemicals comprising putrescine, cadaverine, histamine and tyramine, which are located on the surface of the seafood specimen. The indicator compound is capable of changing the color of the sampling matrix thereby indicating a qualitative visually detectable color change. A method for the nondestructive detection of the quality of a seafood specimen at any given time and for determining the remaining usable shelf life of the seafood specimen is disclosed.
摘要翻译：提供了一种用于定性检测海鲜中的腐败的基于酶的非破坏性传感器，其中传感器不改变海鲜样品的物理组成。传感器包括采样矩阵，与取样矩阵接触的至少三种或更多种酶以及与取样基质接触的至少一种指示剂化合物。这些酶能够与位于海鲜样品表面上的包括腐胺，尸胺，组胺和酪胺的四种目标化学物质相互作用。指示剂化合物能够改变采样基质的颜色，从而指示定性的视觉上可检测的颜色变化。公开了在任何给定时间无损检测海产品样品的质量并确定海产品样品的剩余可用保质期的方法。

2. 发明申请

WO2013074168A1 METHOD AND DEVICE FOR DETECTING, CLASSIFYING, AND IDENTIFYING PARTICLES, AEROSOLS OR VAPORS 审中-公开
标题翻译：用于检测，分类和鉴定颗粒，气溶胶或蒸气的方法和装置
公开(公告)号：WO2013074168A1
公开(公告)日：2013-05-23
申请号：PCT/US2012/052481
申请日：2012-08-27
申请人： AGENTASE, LLC, A SUBSIDIARY OF FLIR SYSTEMS, INC. , ERBELDINGER, Markus , SILCOTT, David B. , BERBERICH, Jason Aaron , LEJEUNE, Keith E.
发明人： ERBELDINGER, Markus , SILCOTT, David B. , BERBERICH, Jason Aaron , LEJEUNE, Keith E.
IPC分类号： C12Q1/00 , G01N21/00 , B01J10/00
CPC分类号： C12Q1/25 , G01N1/2273 , G01N15/0606 , G01N21/6428 , G01N21/766 , G01N33/0013
摘要： A method and device are disclosed for continuously detecting, classifying and identifying toxic particles, aerosols and/ or vapor in an air sample, in near real time by directing an air sample containing an optional target analyte, in the form of particles, aerosols and/or vapors, enzyme(s), and enzyme substrate(s), to a surface of a collection matrix for forming a biocatalytic reaction product of a plurality of freely mobile optical reporters, and by using a light source with optical reader to interpret the signal from the optical reporter, enabling the detection, classification and identification of toxic particles, aerosols and/ or vapor in the air sample.
摘要翻译：公开了一种方法和装置，用于通过将含有任选的目标分析物的空气样品以颗粒，气溶胶和/或气体的形式连续地检测，分类和鉴定空气样品中的有毒颗粒，气溶胶和/或蒸气，或蒸气，酶和酶底物转移到用于形成多个自由移动光学记录器的生物催化反应产物的收集基质的表面，并且通过使用具有光学读取器的光源来解释信号从光学报告员，能够检测，分类和鉴定空气样品中的有毒颗粒，气溶胶和/或蒸气。

3. 发明申请

WO2008036061A3 ENZYMES AND FORMULATIONS FOR BROAD-SPECIFICITY DECONTAMINATION OF CHEMICAL AND BIOLOGICAL WARFARE AGENTS 审中-公开
公开(公告)号：WO2008036061A3
公开(公告)日：2008-03-27
申请号：PCT/US2006/013031
申请日：2006-04-06
申请人： VERENIUM CORPORATION , AGENTASE, LLC , LIFE SCIENCE RESEARCH ISRAEL LTD. , ROBERTSON, Dan, E. , RICHARDSON, Toby , KUSTEDJO, Karen , AMITAI, Gabriel , LEJEUNE, Keith , BERBERICH, Jason , CHAPLIN, Jennifer, Ann , SINCLAIR, Jessica
发明人： ROBERTSON, Dan, E. , RICHARDSON, Toby , KUSTEDJO, Karen , AMITAI, Gabriel , LEJEUNE, Keith , BERBERICH, Jason , CHAPLIN, Jennifer, Ann , SINCLAIR, Jessica
IPC分类号： C12Q1/68
摘要： The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad-spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.

4. 发明申请

WO2006042001A2 STABLE THREE ENZYME CREATININE BIOSENSOR 审中-公开
标题翻译：稳定的三种酶肌酸生物传感器
公开(公告)号：WO2006042001A2
公开(公告)日：2006-04-20
申请号：PCT/US2005/035959
申请日：2005-10-05
申请人： BAYER HEALTHCARE LLC , BERBERICH, Jason , BODEN, Mark, W. , CHAN, Andy, D., C. , RUSSELL, Alan
发明人： BERBERICH, Jason , BODEN, Mark, W. , CHAN, Andy, D., C. , RUSSELL, Alan
CPC分类号： C12Q1/005
摘要： The invention provides methods for preparing a stable, multiple-use three enzyme biosensor for the amperometric determination of creatinine in biological liquids that has a useful lifetime that extends significantly beyond that of presently available amperometric biosensors. The biosensor prepared by the methods of the invention encompasses a plurality of immobilized enzymes that are applied to the biosensor as an enzyme-polymer composition. The enzymes, which can include creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, are immobilized into the enzyme-polymer composition simultaneously as well as applied to the biosensor simultaneously. Prior to being immobilized, the enzymes can be chemically modified by attaching one or more polyethylene glycol (PEG) chains per enzyme monomer. The polymer component can be provided by a polyurethane membrane. The invention also provides a method of preparing a biosensor that limits the diffusion of silver ions emanating the reference electrode, thereby preventing, contact between the silver ions and the enzymes. Related methods of preparing an enzyme-polymer composition for incorporation into a multiple use three enzyme biosensor for the amperometric determination of creatinine in biological liquids also are provided. The invention also provides multiple-use biosensors and enzyme-polymer compositions prepared by the methods disclosed.
摘要翻译：本发明提供了用于制备用于安培测定生物液体中的肌酸酐的稳定的多次使用的三酶生物传感器的方法，所述生物传感器的使用寿命明显超过目前可用的安培计生物传感器。通过本发明的方法制备的生物传感器包含多种作为酶 - 聚合物组合物应用于生物传感器的固定化酶。可以包括肌酸酐酰胺水解酶，肌酸脒基水解酶和肌氨酸氧化酶的酶同时固定在酶 - 聚合物组合物中，并同时应用于生物传感器。在被固定之前，酶可以通过每个酶单体连接一个或多个聚乙二醇（PEG）链来进行化学修饰。聚合物组分可以由聚氨酯膜提供。本发明还提供一种制备生物传感器的方法，该生物传感器限制发射参比电极的银离子的扩散，从而防止银离子与酶之间的接触。还提供了制备用于并入多用途三酶生物传感器的酶 - 聚合物组合物的相关方法，用于安培测定生物液体中的肌酸酐。本发明还提供通过所公开的方法制备的多用途生物传感器和酶 - 聚合物组合物。

5. 发明申请

WO2008076153A3 ENZYME CONTAINING LIQUID AND DELIVERY SYSTEM FOR DETECTION OF ANALYTES ON SURFACES 审中-公开
公开(公告)号：WO2008076153A3
公开(公告)日：2008-06-26
申请号：PCT/US2007/017563
申请日：2007-08-07
申请人： AGENTASE, LLC , LEJEUNE, Keith, E. , BACIOR, Jennifer, A. , BERBERICH, Jason, A. , ERBELDINGER, Markus , LELE, Bhalchandra, Shripad
发明人： LEJEUNE, Keith, E. , BACIOR, Jennifer, A. , BERBERICH, Jason, A. , ERBELDINGER, Markus , LELE, Bhalchandra, Shripad
IPC分类号： C12Q1/34 , C12Q1/68 , C12M3/00
摘要： An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.

6. 发明申请

WO2008076153A2 ENZYME CONTAINING LIQUID AND DELIVERY SYSTEM FOR DETECTION OF ANALYTES ON SURFACES 审中-公开
标题翻译：用于检测表面分析物的含酶液体和输送系统
公开(公告)号：WO2008076153A2
公开(公告)日：2008-06-26
申请号：PCT/US2007017563
申请日：2007-08-07
申请人： AGENTASE LLC , LEJEUNE KEITH E , BACIOR JENNIFER A , BERBERICH JASON A , ERBELDINGER MARKUS , LELE BHALCHANDRA SHRIPAD
发明人： LEJEUNE KEITH E , BACIOR JENNIFER A , BERBERICH JASON A , ERBELDINGER MARKUS , LELE BHALCHANDRA SHRIPAD
IPC分类号： C12M3/00
CPC分类号： C12Q1/28 , C12Q1/34 , C12Q1/37 , C12Q1/46
摘要： An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.
摘要翻译：一种含水酶的传感器，其包含一种或多种酶和至少一种能够指示至少一种酶与至少一种目标分析物的反应的指示剂化合物，其中酶和指示剂能够被递送，优选通过喷射到具有目标分析物的表面，而不对表面进行取样。提供了一种基于水性酶的传感器，其能够检测作为分析物的酶特异性底物。该基于水性酶的传感器还包含一种或多种酶特异性底物，其中传感器检测酶抑制。基于水性酶的传感器任选地包含一种或多种光散射添加剂，粘合剂聚合物，蛋白稳定剂，蛋白质稳定糖，表面活性剂和溶剂（s）及其组合。提供了一种分配系统和一种在不对表面进行取样的情况下在表面上检测目标化学物质的方法。

7. 发明申请

WO2008036061A2 ENZYMES AND FORMULATIONS FOR BROAD-SPECIFICITY DECONTAMINATION OF CHEMICAL AND BIOLOGICAL WARFARE AGENTS 审中-公开
标题翻译：化学和生物保护剂广泛消除的酶和制剂
公开(公告)号：WO2008036061A2
公开(公告)日：2008-03-27
申请号：PCT/US2006013031
申请日：2006-04-06
申请人： VERENIUM CORP , AGENTASE LLC , LIFE SCIENCE RES ISRAEL LTD , ROBERTSON DAN E , RICHARDSON TOBY , KUSTEDJO KAREN , AMITAI GABRIEL , LEJEUNE KEITH , BERBERICH JASON , CHAPLIN JENNIFER ANN , SINCLAIR JESSICA
发明人： ROBERTSON DAN E , RICHARDSON TOBY , KUSTEDJO KAREN , AMITAI GABRIEL , LEJEUNE KEITH , BERBERICH JASON , CHAPLIN JENNIFER ANN , SINCLAIR JESSICA
IPC分类号： A62D3/02 , A62D101/04
CPC分类号： A62D3/02 , A62D2101/02 , C12N9/00 , C12N9/16
摘要： The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad-spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.
摘要翻译：本发明提供用于酶的活性去污的新型酶和方法，例如神经毒素解毒。一方面，本发明提供了具有非血红素氯过氧化物酶（nhCPOs）活性的酶以及木质素的漂白和降解方法。本发明提供多肽，核酸，感染性载体，转导或感染的细胞或植物和/或转基因植物及其使用方法，例如提供自我保护，抗农药和/或除草剂抗性细胞或植物，其中在一个方面，本发明的多肽起到水解PS或PF键并使乙酰胆碱酯酶或丁酰胆碱酯酶解毒的作用。一方面，本发明提供了提供广谱，试剂特异性氧化和水解活性的酶混合物或组合物，以及用于氧化或水解V-剂，G-剂和H-剂并产生氧化剂的次卤酸盐例如用于杀死生物制剂（包括炭疽孢子）的二氧化氯和反应性基团。

8. 发明申请

WO2006042001A3 STABLE THREE ENZYME CREATININE BIOSENSOR 审中-公开
标题翻译：稳定的三烯酸生物传感器
公开(公告)号：WO2006042001A3
公开(公告)日：2006-07-13
申请号：PCT/US2005035959
申请日：2005-10-05
申请人： BAYER HEALTHCARE LLC , BERBERICH JASON , BODEN MARK W , CHAN ANDY D C , RUSSELL ALAN
发明人： BERBERICH JASON , BODEN MARK W , CHAN ANDY D C , RUSSELL ALAN
IPC分类号： C12Q1/00 , G01N27/26 , G01N33/50
CPC分类号： C12Q1/005
摘要： The invention provides methods for preparing a stable, multiple-use three enzyme biosensor for the amperometric determination of creatinine in biological liquids that has a useful lifetime that extends significantly beyond that of presently available amperometric biosensors. The biosensor prepared by the methods of the invention encompasses a plurality of immobilized enzymes that are applied to the biosensor as an enzyme-polymer composition. The enzymes, which can include creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, are immobilized into the enzyme-polymer composition simultaneously as well as applied to the biosensor simultaneously. Prior to being immobilized, the enzymes can be chemically modified by attaching one or more polyethylene glycol (PEG) chains per enzyme monomer. The polymer component can be provided by a polyurethane membrane. The invention also provides a method of preparing a biosensor that limits the diffusion of silver ions emanating the reference electrode, thereby preventing, contact between the silver ions and the enzymes. Related methods of preparing an enzyme-polymer composition for incorporation into a multiple use three enzyme biosensor for the amperometric determination of creatinine in biological liquids also are provided. The invention also provides multiple-use biosensors and enzyme-polymer compositions prepared by the methods disclosed.
摘要翻译：本发明提供了制备稳定的多用途三酶生物传感器的方法，所述三维酶生物传感器用于生物液体中肌酸酐的电流分析测定，所述生物液体的使用寿命显着超过目前可用的电流计生物传感器的使用寿命。通过本发明的方法制备的生物传感器包括作为酶 - 聚合物组合物应用于生物传感器的多种固定化酶。可以将肌酸酐酰氨基水解酶，肌酸脒基水解酶和肌氨酸氧化酶的酶同时固定在酶 - 聚合物组合物中，同时应用于生物传感器。在固定化之前，可以通过每个酶单体连接一个或多个聚乙二醇（PEG）链来化学修饰酶。聚合物组分可以由聚氨酯膜提供。本发明还提供一种制备生物传感器的方法，其限制了散发参考电极的银离子的扩散，从而防止银离子与酶之间的接触。还提供了制备用于并入多用途三酶生物传感器中的酶 - 聚合物组合物的相关方法，用于生物液体中肌酸酐的电流分析测定。本发明还提供了通过所公开方法制备的多用途生物传感器和酶 - 聚合物组合物。

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