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1. 发明申请

WO2008085296A1 SEAFOOD SPOILAGE INDICATOR 审中-公开
标题翻译：海鲜指示器
公开(公告)号：WO2008085296A1
公开(公告)日：2008-07-17
申请号：PCT/US2007/025624
申请日：2007-12-14
申请人： AGENTASE, LLC , LEECH, Anna, M. , PALUMBO, Jessica, L. , BERBERICH, Jason, A. , LEJEUNE, Keith, E.
发明人： LEECH, Anna, M. , PALUMBO, Jessica, L. , BERBERICH, Jason, A. , LEJEUNE, Keith, E.
IPC分类号： G01N33/02 , C12M3/00 , C12Q1/28 , C12Q1/26
CPC分类号： G01N33/02
摘要： An enzyme based nondestructive sensor for the qualitative detection of spoilage in seafood is provided wherein the sensor does not alter the physical composition of the seafood specimen. The sensor comprises a sampling matrix, at least three or more enzymes in contact with the sampling matrix, and at least one indicator compound in contact with the sampling matrix. The enzymes are capable of interacting with four target chemicals comprising putrescine, cadaverine, histamine and tyramine, which are located on the surface of the seafood specimen. The indicator compound is capable of changing the color of the sampling matrix thereby indicating a qualitative visually detectable color change. A method for the nondestructive detection of the quality of a seafood specimen at any given time and for determining the remaining usable shelf life of the seafood specimen is disclosed.
摘要翻译：提供了一种用于定性检测海鲜中的腐败的基于酶的非破坏性传感器，其中传感器不改变海鲜样品的物理组成。传感器包括采样矩阵，与取样矩阵接触的至少三种或更多种酶以及与取样基质接触的至少一种指示剂化合物。这些酶能够与位于海鲜样品表面上的包括腐胺，尸胺，组胺和酪胺的四种目标化学物质相互作用。指示剂化合物能够改变采样基质的颜色，从而指示定性的视觉上可检测的颜色变化。公开了在任何给定时间无损检测海产品样品的质量并确定海产品样品的剩余可用保质期的方法。

2. 发明申请

WO2008076153A3 ENZYME CONTAINING LIQUID AND DELIVERY SYSTEM FOR DETECTION OF ANALYTES ON SURFACES 审中-公开
公开(公告)号：WO2008076153A3
公开(公告)日：2008-06-26
申请号：PCT/US2007/017563
申请日：2007-08-07
申请人： AGENTASE, LLC , LEJEUNE, Keith, E. , BACIOR, Jennifer, A. , BERBERICH, Jason, A. , ERBELDINGER, Markus , LELE, Bhalchandra, Shripad
发明人： LEJEUNE, Keith, E. , BACIOR, Jennifer, A. , BERBERICH, Jason, A. , ERBELDINGER, Markus , LELE, Bhalchandra, Shripad
IPC分类号： C12Q1/34 , C12Q1/68 , C12M3/00
摘要： An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.

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