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11. 发明申请

WO2006043895A1 A METHOD OF ANTIBODY PURIFICATION 审中-公开
标题翻译：一种抗体纯化方法
公开(公告)号：WO2006043895A1
公开(公告)日：2006-04-27
申请号：PCT/SE2005/001591
申请日：2005-10-21
申请人： GE HEALTHCARE BIO-SCIENCES AB , ENGSTRAND, Carina , FORSS, Annika , GLAD, Gunnar , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc
发明人： ENGSTRAND, Carina , FORSS, Annika , GLAD, Gunnar , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc
IPC分类号： B01J41/04 , B01D15/32 , B01D15/36 , C07K16/00
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface
摘要翻译：本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不期望的化合物，同时抗体在液体中保持游离，其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。本发明还涉及一种填充有上述多模式分离基质的色谱柱和一种具有吸附在其表面上的多模式基团的过滤器

12. 发明申请

WO2009145722A1 SEPARATION METHOD UTILIZING POLYALLYLAMINE LIGANDS 审中-公开
标题翻译：利用聚氨酯配体的分离方法
公开(公告)号：WO2009145722A1
公开(公告)日：2009-12-03
申请号：PCT/SE2009/050622
申请日：2009-05-29
申请人： GE HEALTHCARE BIO-SCIENCES AB , JOHANSSON, Bo-Lennart , KARLSSON, Ulrika , MALOISEL, Jean-Luc
发明人： JOHANSSON, Bo-Lennart , KARLSSON, Ulrika , MALOISEL, Jean-Luc
IPC分类号： B01D15/36 , B01J20/26 , B01J41/14 , G01N30/34 , B01J47/00
CPC分类号： B01D15/363 , B01J20/3242 , B01J20/327 , B01J41/20 , G01N30/96
摘要： The present invention relates to a methodfor removing at least one negatively charged substance from an aqueous liquid by contacting the liquid with a separation matrix comprising a plurality of polyallylamine ligands, comprising binding said negatively charged substance to said ligands under conditions where the ionic strength of the aqueous liquid applied to the chromatographyresin ≥0.25 M NaCl.
摘要翻译：本发明涉及通过使液体与包含多个聚烯丙基胺配体的分离基质接触来从含水液体中除去至少一种带负电荷的物质的方法，包括在所述配位体的离子强度水性液体施加到色谱分离蛋白= 0.25M NaCl。

13. 发明申请

WO2009131526A1 CHROMATOGRAPHY MEDIUM 审中-公开
标题翻译：色谱介质
公开(公告)号：WO2009131526A1
公开(公告)日：2009-10-29
申请号：PCT/SE2009/050406
申请日：2009-04-21
申请人： GE HEALTHCARE BIO-SCIENCES AB , BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , SÖDERMAN, Tobias, E.
发明人： BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , SÖDERMAN, Tobias, E.
IPC分类号： B01J20/285 , B01D15/34
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation mediumfor purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
摘要翻译：本发明在色谱领域内。更准确地说，它涉及一种新颖的色谱介质，即由于疏水介质的孔隙率和/或盖的孔隙度，提供了不同盖子的疏水介质，其中排除了一定尺寸的分子。本发明还涉及用于纯化不分离介质的大分子的分离介质的使用以及进入分离介质并从其中洗脱的小分子。

14. 发明申请

WO2009099375A1 METHOD FOR PRODUCTION OF SEPARATION MEDIA 审中-公开
标题翻译：分离介质的生产方法
公开(公告)号：WO2009099375A1
公开(公告)日：2009-08-13
申请号：PCT/SE2009/000063
申请日：2009-01-30
申请人： GE HEALTHCARE BIO-SCIENCES AB , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , STENHOLM, Åke
发明人： GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils , STENHOLM, Åke
IPC分类号： B01J20/285 , B01J2/14
CPC分类号： B01J20/291 , B01J20/262 , B01J20/2809 , B01J20/285 , B01J20/286 , B01J20/287 , B01J20/288 , B01J20/3212 , B01J20/3268 , B01J20/3274 , B01J20/3293 , B01J39/26 , B01J41/20 , B01J2220/52
摘要： The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75°C to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27°C, preferably between 17.5 and 24.6°C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.
摘要翻译：本发明涉及使用所谓的旋转圆盘技术生产分离介质的方法，其中珠子的孔隙度以使得所需生物分子可以与复杂样品分离的方式进行优化。该方法包括以下步骤：a）将具有350-450mPa·s粘度的4-8％多糖溶液在65-75℃下以3001-3010rpm向一个或多个纺丝盘送入以形成多糖珠; b）在捕获浴中捕获所述形成的多糖珠粒; 其中多糖珠的孔隙率通过在15和27℃之间，优选在17.5和24.6℃之间改变捕获温度来控制。该方法产生阻止大于150000g / mol的分子扩散到珠中的孔隙率。本发明还涉及通过其用于纯化生物分子，特别是单克隆抗体的方法和用途产生的分离培养基。

15. 发明申请

WO2008121042A1 PURIFICATION OF IMMUNOGLOBULINS 审中-公开
标题翻译：免疫球蛋白的纯化
公开(公告)号：WO2008121042A1
公开(公告)日：2008-10-09
申请号：PCT/SE2008/000212
申请日：2008-03-20
申请人： GE HEALTHCARE BIO-SCIENCES AB , MALOISEL, Jean-Luc , JOHANSSON, Bo-Lennart
发明人： MALOISEL, Jean-Luc , JOHANSSON, Bo-Lennart
IPC分类号： B01J20/289 , B01D15/32
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
摘要翻译：本发明涉及由已经固定有配体的多孔或无孔载体组成的分离基质，其中所述配体包含至少一个脂族磺酰胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白如IgG，Fab片段，含有免疫球蛋白的融合蛋白等的方法的本发明。

16. 发明申请

WO2004048569A1 ISOLATION OF ANTISENSE OLIGONUCLEOTIDES 审中-公开
标题翻译：分离反义寡核苷酸
公开(公告)号：WO2004048569A1
公开(公告)日：2004-06-10
申请号：PCT/SE2003/001784
申请日：2003-11-17
申请人： AMERSHAM BIOSCIENCES AB , ERIKSSON, Kjell , JOHANSSON, Bo-Lennart
发明人： ERIKSSON, Kjell , JOHANSSON, Bo-Lennart
IPC分类号： C12N15/10
CPC分类号： B01D15/3828 , B01J45/00 , C07H1/06 , C07H1/08
摘要： The present invention relates to a method of isolating fully thioated single stranded antisense oligonucleotides from a biological solution, which method comprises the steps of contacting the biological solution with an immobilised metal ion adsorption chromatography (IMAC) resin to adsorb the antisense oligonucleotides to said resin and subsequently contacting the resin with an eluent under conditions that provide desorption of the antisense oligonucleotides from said resin, wherein the fully thioated antisense oligonucleotides are separated from incorrectly thioated antisense oligonucleotides in said solution. The invention also relates to the use of an immobilised metal ion adsorption chromatography (IMAC) resin for isolation of fully thioated single stranded antisense oligonucleotides from a biological solution.
摘要翻译：本发明涉及从生物溶液中分离完全硫酸化的单链反义寡核苷酸的方法，该方法包括使生物溶液与固定金属离子吸附色谱（IMAC）树脂接触以将反义寡核苷酸吸附到所述树脂和随后在从所述树脂提供反义寡核苷酸解吸的条件下将树脂与洗脱液接触，其中完全硫代反义寡核苷酸与所述溶液中的错误地硫代反义寡核苷酸分离。本发明还涉及固定化金属离子吸附色谱（IMAC）树脂从生物溶液中分离完全硫代单链反义寡核苷酸的用途。

17. 发明申请

WO2011136721A1 METHOD FOR PRODUCTION OF CHROMATOGRAPHY MEDIA 审中-公开
标题翻译：生产色谱介质的方法
公开(公告)号：WO2011136721A1
公开(公告)日：2011-11-03
申请号：PCT/SE2011/050481
申请日：2011-04-20
申请人： GE HEALTHCARE BIO-SCIENCES AB , BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
发明人： BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
IPC分类号： B01J20/285 , B01D15/18 , G01N30/36
CPC分类号： B01J39/165 , B01J20/28004 , B01J20/286 , B01J20/3242 , B01J20/3293 , B01J39/17 , B01J47/018
摘要： The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalisation as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D 50V for large particles/ D 50V for small particles] >1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beds/Total volume beads] is in the range 0.05 –0.9.
摘要翻译：本发明涉及产生具有改进的压力 - 流动性质的色谱介质的方法和方法。本发明更紧密地涉及双峰粒度分布和层官能化用作改变色谱介质压力流动性质的手段。本发明涉及一种用于制备具有改进的压力 - 流动性质的色谱介质的方法，包括将具有较小珠粒/颗粒的包含内核和外部功能化壳/盖的大珠粒/颗粒混合，其中颗粒尺寸大颗粒和小珠：[D50V为大颗粒/ D50V为小颗粒]> 1.2，其中柱中大小珠的体积比为[大床/总体积珠的总体积]在0.05 -0.9。

18. 发明申请

WO2009014481A1 SEPARATION MATRIX 审中-公开
标题翻译：分离矩阵
公开(公告)号：WO2009014481A1
公开(公告)日：2009-01-29
申请号：PCT/SE2008/000461
申请日：2008-07-22
申请人： GE HEALTHCARE BIO-SCIENCES AB , AXÉN, Andreas , BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , NORRMAN, Nils
发明人： AXÉN, Andreas , BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , NORRMAN, Nils
IPC分类号： B01J20/289
CPC分类号： B01J20/289 , B01D15/362 , B01D15/363 , B01J20/3274 , B01J20/328
摘要： The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.
摘要翻译：本发明涉及一种分离基质，其包括载体; 联接到所述支撑件的外部的延伸器; 和与所述增量剂偶联的配体，其中与所述增量剂偶联的载体的部分构成小于分离基质体积的50％。本发明还包括制备这种分离基质的方法，以及其中使用分离介质的方法。

19. 发明申请

WO2012074468A1 BUFFERING COMPOSITIONS ENCLOSED IN A SIZE EXCLUSION MATRIX 审中-公开
标题翻译：缓冲组合物在一个尺寸排除矩阵中
公开(公告)号：WO2012074468A1
公开(公告)日：2012-06-07
申请号：PCT/SE2011/051439
申请日：2011-11-29
申请人： GE HEALTHCARE BIO-SCIENCES AB , BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
发明人： BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
IPC分类号： B01J20/285 , B01J20/32
CPC分类号： G01N1/28 , B01J20/28009 , B01J20/286 , B01J20/3208 , B01J20/3217 , B01J20/327 , B01J20/3274 , B01J20/3285 , B01J20/3293 , Y10T436/2525
摘要： The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment. The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of
摘要翻译：本发明涉及一种缓冲组合物，更接近地包含当在例如水性环境中施用时提供pH稳定化的多孔基质封闭的缓冲剂的组合物。组合物包含封闭在第一多孔基质中的缓冲剂，其不透性对应于球状蛋白和肽的分数范围<10000g / mol，优选<5000g / mol，更优选<700g / mol。

20. 发明申请

WO2012005664A1 CHROMATOGRAPHY METHOD AND MEDIA USED THEREFORE 审中-公开
标题翻译：使用的色谱方法和介质
公开(公告)号：WO2012005664A1
公开(公告)日：2012-01-12
申请号：PCT/SE2011/050852
申请日：2011-06-28
申请人： GE HEALTHCARE BIO-SCIENCES AB , BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
发明人： BERGSTRÖM, Jan , JOHANSSON, Bo-Lennart
IPC分类号： B01J20/285 , B01D15/36 , B01J39/26 , B01J41/20 , C07K1/18
CPC分类号： B01D15/361 , C07K1/18
摘要： The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.
摘要翻译：本发明涉及一种用于在包含具有内多孔芯和外壳的壳珠的介质上运行离子交换色谱的方法，其中内核具有其电荷随pH变化并且壳被带有带电离子交换的配体配体，该方法包括以下步骤：a）在第一pH下将样品分子吸附在壳配体上; b）通过添加能够增加其与核配体相同符号/类型的电荷的缓冲物质，在第二pH下引起内核配体的排出，同时使离子从内部核心配体，从而使离子强度的增加从壳体配体取代样品分子即引起洗脱。

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