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1. 发明申请

WO2009145722A1 SEPARATION METHOD UTILIZING POLYALLYLAMINE LIGANDS 审中-公开
标题翻译：利用聚氨酯配体的分离方法
公开(公告)号：WO2009145722A1
公开(公告)日：2009-12-03
申请号：PCT/SE2009/050622
申请日：2009-05-29
申请人： GE HEALTHCARE BIO-SCIENCES AB , JOHANSSON, Bo-Lennart , KARLSSON, Ulrika , MALOISEL, Jean-Luc
发明人： JOHANSSON, Bo-Lennart , KARLSSON, Ulrika , MALOISEL, Jean-Luc
IPC分类号： B01D15/36 , B01J20/26 , B01J41/14 , G01N30/34 , B01J47/00
CPC分类号： B01D15/363 , B01J20/3242 , B01J20/327 , B01J41/20 , G01N30/96
摘要： The present invention relates to a methodfor removing at least one negatively charged substance from an aqueous liquid by contacting the liquid with a separation matrix comprising a plurality of polyallylamine ligands, comprising binding said negatively charged substance to said ligands under conditions where the ionic strength of the aqueous liquid applied to the chromatographyresin ≥0.25 M NaCl.
摘要翻译：本发明涉及通过使液体与包含多个聚烯丙基胺配体的分离基质接触来从含水液体中除去至少一种带负电荷的物质的方法，包括在所述配位体的离子强度水性液体施加到色谱分离蛋白= 0.25M NaCl。

2. 发明申请

WO2005059116A1 METHODS FOR CLONAL DERIVATION OF HUMAN BLASTOCYST-DERIVED STEM CELL LINES 审中-公开
标题翻译：人造血干细胞移植细胞系的克隆方法
公开(公告)号：WO2005059116A1
公开(公告)日：2005-06-30
申请号：PCT/EP2004/014016
申请日：2004-12-08
申请人： CELLARTIS AB , LINDAHL, Anders , KARLSSON, Ulrika
发明人： LINDAHL, Anders , KARLSSON, Ulrika
IPC分类号： C12N5/02
CPC分类号： C12N5/0606 , C12N2501/115 , C12N2502/13 , C12N2509/00
摘要： Human blastocyst-derived stem cells (hBS) or hBS derived cells, such as, e.g., cells of endodermal, mesodermal, and ectodermal origin, are pluripotent cells with widespread potentials within the areas of e.g. therapeutic treatment, human developmental biology, and drug discovery processes. The present invention relates to a method for clonal derivation of human blastocyst-derived stem cells (hBS) or hBS derived cells. According to the present invention hBS cell colonies or hBS derived cell colonies are subjected to non-enzymatic treatment for dissociation of the cell colonies to one or more single cells, which are then separately cultivated in a serum based medium and/or serum based conditioned medium to obtain one or more cell clones capable of forming colonies.
摘要翻译：人胚泡来源的干细胞（hBS）或hBS衍生的细胞，例如内胚层，中胚层和外胚层来源的细胞，是具有广泛潜力的多能细胞。治疗性治疗，人类发育生物学和药物发现过程。本发明涉及一种用于人源胚泡干细胞（hBS）或hBS衍生细胞克隆衍生的方法。根据本发明，将hBS细胞集落或hBS衍生的细胞集落进行非酶处理以将细胞集落解离成一个或多个单细胞，然后将其单独培养在基于血清的培养基和/或血清基条件培养基以获得能够形成菌落的一个或多个细胞克隆。

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