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1. 发明申请

US20100151454A1 MICROFLUIDIC APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING MOLECULAR REACTIONS 审中-公开
标题翻译：用于实施分子反应的微流感设备，系统和方法
公开(公告)号：US20100151454A1
公开(公告)日：2010-06-17
申请号：US12256409
申请日：2008-10-22
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

2. 发明申请

US20090170725A1 Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
标题翻译：使用肽或核酸微图案生产碳纳米管的方法
公开(公告)号：US20090170725A1
公开(公告)日：2009-07-02
申请号：US11344713
申请日：2006-01-31
申请人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
发明人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
IPC分类号： C40B40/08 , B82B1/00
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.
摘要翻译：本文公开的方法，装置和系统涉及碳纳米管的有序阵列。在本发明的具体实施方案中，纳米管阵列通过包括将催化剂纳米颗粒140,230连接到聚合物120,210分子，将聚合物120,210分子连接到基底上的方法形成，除去聚合物120,210分子并产生碳催化剂纳米颗粒140,230上的纳米管。聚合物120,210分子可以使用自组装或分子对准技术以有序图案附着到基底上。纳米管阵列可以附着到基板的选定区域110,310。在所选择的区域110,310内，纳米管是非随机分布的。本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列和包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统的装置。在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

3. 发明授权

US07442339B2 Microfluidic apparatus, Raman spectroscopy systems, and methods for performing molecular reactions 有权
标题翻译：微流体装置，拉曼光谱系统和进行分子反应的方法
公开(公告)号：US07442339B2
公开(公告)日：2008-10-28
申请号：US10815264
申请日：2004-03-31
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
IPC分类号： G01N21/65 , G01J3/44 , G01N33/48
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

4. 发明授权

US07291466B2 Detecting molecular binding by monitoring feedback controlled cantilever deflections 有权
标题翻译：通过监测反馈控制的悬臂偏转来检测分子结合
公开(公告)号：US07291466B2
公开(公告)日：2007-11-06
申请号：US11111308
申请日：2005-04-20
申请人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
发明人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , B82Y5/00 , G01N33/54373 , G01N2800/52 , Y10S977/924 , C12Q2565/501
摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
摘要翻译：本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。将探针与分析物结合导致悬臂的偏转，由检测单元检测。可以应用平衡力将悬臂恢复到其原始位置。平衡力可以是磁性的，电的或辐射的。生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

5. 发明授权

US07005264B2 Method and apparatus for nucleic acid sequencing and identification 有权
标题翻译：用于核酸测序和鉴定的方法和装置
公开(公告)号：US07005264B2
公开(公告)日：2006-02-28
申请号：US10153125
申请日：2002-05-20
申请人： Xing Su , Andrew A. Berlin
发明人： Xing Su , Andrew A. Berlin
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N33/48721 , B01L3/5027 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2563/155 , C12Q2527/113
摘要： The methods and apparatus 100 disclosed herein are of use for sequencing and/or identifying nucleic acids 230, 310. Nucleic acids 230, 310 containing labeled nucleotides 235, 245, 315 may be synthesized and passed through nanopores 255, 310. Detectors 257, 345 operably coupled to the nanopores 255, 310 may detect the labeled nucleotides 235, 245, 315. By determining the time intervals at which labeled nucleotides 235, 245, 315 are detected, distance maps 140 for each type of labeled nucleotide 235, 245, 315 may be compiled. The distance maps 140 in turn may be used to sequence 150 and/or identify 160 the nucleic acid 230, 310. In different embodiments of the invention, luminescent nucleotides 235, 245 or nanoparticles 315 may be detected using photodetectors 257 or electrical detectors 310. Apparatus 100 and sub-devices 200, 300 of use for nucleic acid 230, 310 sequencing 150 and/or identification 160 are also disclosed herein.
摘要翻译：本文公开的方法和装置100可用于测序和/或鉴定核酸230,310。可以合成含有标记核苷酸235,245,315的核酸230,310并通过纳米孔255,310。可操作地偶联到纳米孔255,310的检测器257,345可以检测标记的核苷酸235,245,315。通过确定检测到标记核苷酸235,245,315的时间间隔，可以编译每种类型的标记核苷酸235,245,315的距离图140。距离图140依次可用于对核酸230,310进行序列150和/或识别160。在本发明的不同实施方案中，可以使用光电检测器257或电检测器310检测发光核苷酸235,245或纳米颗粒315。本文还公开了用于核酸230,310测序150和/或识别160的装置100和子装置200,300。

6. 发明授权

US07744816B2 Methods and device for biomolecule characterization 有权
标题翻译：生物分子表征的方法和装置
公开(公告)号：US07744816B2
公开(公告)日：2010-06-29
申请号：US10138157
申请日：2002-05-01
申请人： Xing Su , Andrew A. Berlin
发明人： Xing Su , Andrew A. Berlin
IPC分类号： G01N33/48
CPC分类号： G01N33/48721 , C12Q2563/179 , C12Q2565/631 , G01N33/6803
摘要： The methods and apparatus 100, disclosed herein are of use for sequencing 150 and/or identifying 160 proteins 230, 310, polypeptides 230, 310 or peptides 230, 310. Proteins 230, 310 containing labeled amino acid residues may be synthesized and passed through nanopores 255, 330. A detector 257, 345 operably coupled to a nanopore 255, 330 may detect labeled amino acid residues as they pass through the nanopore 255, 330. Distance maps 140 for each type of labeled amino acid residue may be compiled. The distance maps 140 may be used to sequence 150 and/or identify 160 the protein 230, 310. In different embodiments of the invention, amino acid residues labeled with luminescent labels 235, 245 or nanoparticles 315 may be detected using photodetectors 257 or electrical detectors 345. Apparatus 100 of use for protein 230, 310 sequencing 150 and/or identification 160 are also disclosed herein.
摘要翻译：本文公开的方法和装置100可用于测序150和/或鉴定160个蛋白质230,310，多肽230,310或肽230,310。可以合成含有标记的氨基酸残基的蛋白质230,310并通过纳米孔可操作地耦合到纳米孔255,330的检测器257,345可以在标记的氨基酸残基通过纳米孔255,330时检测标记的氨基酸残基。可以编译每种类型的标记的氨基酸残基的距离图140。距离图140可以用于序列150和/或识别160蛋白230,310。在本发明的不同实施方案中，用发光标记235,245或纳米颗粒315标记的氨基酸残基可以使用光电检测器257或电检测器本文还公开了用于蛋白质230,3001测序150和/或鉴定物160的装置100。

7. 发明申请

US20090169466A1 Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
公开(公告)号：US20090169466A1
公开(公告)日：2009-07-02
申请号：US11344712
申请日：2006-01-31
申请人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
发明人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
IPC分类号： D01F9/12 , C07H21/04
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

8. 发明授权

US07238477B2 Methods to increase nucleotide signals by Raman scattering 有权
标题翻译：通过拉曼散射增加核苷酸信号的方法
公开(公告)号：US07238477B2
公开(公告)日：2007-07-03
申请号：US10660902
申请日：2003-09-12
申请人： Xing Su , Andrew A. Berlin , Selena Chan , Steven J. Kirch , Tac-Woong Koo , Gabi Neubauer , Valluri Rao , Narayanan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Andrew A. Berlin , Selena Chan , Steven J. Kirch , Tac-Woong Koo , Gabi Neubauer , Valluri Rao , Narayanan Sundararajan , Mineo Yamakawa
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , G01N21/63
CPC分类号： C12Q1/6869 , C12Q2565/632 , C12Q2563/155 , C12Q2521/319
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
摘要翻译：本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。在本发明的某些实施方案中，在掺入核酸之前，核苷酸与拉曼标记物共价连接。在其它实施方案中，使用未标记的核酸。核酸外切核酸处理导致通过拉曼光谱法检测的标记或未标记的核苷酸的释放。在本发明的替代实施方案中，通过外切核酸酶处理从核酸释放的核苷酸与纳米颗粒共价交联，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干反斯托克斯拉曼光谱学（CARS）。本发明的其它实施方案涉及用于核酸测序的装置。

9. 发明授权

US07270952B2 Detecting molecular binding by monitoring feedback controlled cantilever deflections 有权
公开(公告)号：US07270952B2
公开(公告)日：2007-09-18
申请号：US10254201
申请日：2002-09-24
申请人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
发明人： Xing Su , Selena Chan , Tae-Woong Koo , Mineo Yamakawa , Andrew A. Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , B82Y5/00 , G01N33/54373 , G01N2800/52 , Y10S977/924 , C12Q2565/501
摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

10. 发明授权

US06972173B2 Methods to increase nucleotide signals by raman scattering 有权
标题翻译：通过拉曼散射增加核苷酸信号的方法
公开(公告)号：US06972173B2
公开(公告)日：2005-12-06
申请号：US10099287
申请日：2002-03-14
申请人： Xing Su , Selena Chan , Andrew A. Berlin , Tae-Woong Koo , Narayan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Selena Chan , Andrew A. Berlin , Tae-Woong Koo , Narayan Sundararajan , Mineo Yamakawa
IPC分类号： G01N21/65 , B01L3/00 , C12M1/00 , C12Q1/68 , G01N33/543 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q1/6872 , C12Q2565/632 , C12Q2563/155 , C12Q2521/319
摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.
摘要翻译：本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。在本发明的某些实施方案中，核苷酸在掺入核酸13之前共价连接到拉曼标记上。标记核酸13的核酸外切酶15处理导致通过拉曼光谱法检测的标记核苷酸16,130的释放。在本发明的替代实施方案中，通过核酸外切酶15处理从核酸13释放的核苷酸16,130共价交联到银或金纳米颗粒140上，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS ）和/或相干的反斯托克斯拉曼光谱（CARS）。本发明的其它实施方案涉及用于核酸测序的装置10,100,210。

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