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1. 发明授权

US09593315B2 Mutant RB69 DNA polymerase 有权
标题翻译：突变型RB69 DNA聚合酶
公开(公告)号：US09593315B2
公开(公告)日：2017-03-14
申请号：US15200670
申请日：2016-07-01
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Marian Peris , Michael Phelan , Barnett Rosenblum , Stephen Hendricks
IPC分类号： C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
摘要翻译：本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。这些突变型聚合酶能够选择性地结合标记的核苷酸。这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

2. 发明申请

US20160304844A1 MUTANT RB69 DNA POLYMERASE 有权
标题翻译： MUTANT RB69 DNA聚合酶
公开(公告)号：US20160304844A1
公开(公告)日：2016-10-20
申请号：US15200670
申请日：2016-07-01
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Marian PERIS , Michael PHELAN , Barnett ROSENBLUM , Stephen HENDRICKS
IPC分类号： C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
摘要翻译：本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。这些突变型聚合酶能够选择性地结合标记的核苷酸。这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

3. 发明授权

US09255258B2 Nucleotide transient binding for sequencing methods 有权
标题翻译：测序方法的核苷酸瞬变结合
公开(公告)号：US09255258B2
公开(公告)日：2016-02-09
申请号：US14108166
申请日：2013-12-16
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Peter Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett Rosenblum , Michael Phelan
IPC分类号： C12Q1/68 , C12N9/12
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

4. 发明申请

US20140329237A1 MEANS AND METHODS FOR ASSESSING SPERM NUCLEAR DNA STRUCTURE 审中-公开
标题翻译：用于评估细胞核DNA结构的方法和方法
公开(公告)号：US20140329237A1
公开(公告)日：2014-11-06
申请号：US14352682
申请日：2011-10-30
申请人： Constantine Mallidis , Stefan Schlatt , Joachim Wistuba , Carsten Fallnich , Petra Gross , Martin Burger , Frank Wuebbeling
发明人： Constantine Mallidis , Stefan Schlatt , Joachim Wistuba , Carsten Fallnich , Petra Gross , Martin Burger , Frank Wuebbeling
IPC分类号： G01N21/65
CPC分类号： G01N21/658 , C12Q1/68 , C12Q1/6883 , C12Q2565/632
摘要： The present invention is directed to a method for determining nuclear DNA (nDNA) in sperm sample by means of Raman micro-spectroscopy. In particular, the method is useful to determine the presence of intact and/or damaged nDNA in a sperm. Further, the present invention is directed to a method for screening sperm for in vitro fertilization or artificial reproduction treatment (ART).
摘要翻译：本发明涉及通过拉曼微光谱法测定精子样品中的核DNA（nDNA）的方法。特别地，该方法可用于确定精子中完整和/或损坏的nDNA的存在。此外，本发明涉及筛选用于体外受精或人造繁殖处理（ART）的精子的方法。

5. 发明申请

US20140234853A1 NUCLEOTIDE TRANSIENT BINDING FOR SEQUENCING METHODS 有权
标题翻译：用于测序方法的核心瞬态绑定
公开(公告)号：US20140234853A1
公开(公告)日：2014-08-21
申请号：US14108166
申请日：2013-12-16
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Peter VANDER HORN , Cheng-Yao CHEN , Guobin LUO , Michael PREVITE , Jamshid TEMIROV , Theo NIKIFOROV , Zhaohui ZHOU , Hongye SUN , Yufang WANG , Stefanie Yukiko NISHIMURA , Hongyi WANG , Marian PERIS , Barnett ROSENBLUM , Michael PHELAN
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

6. 发明申请

US20120250003A1 OPTICAL DETECTION SYSTEM 有权
标题翻译：光学检测系统
公开(公告)号：US20120250003A1
公开(公告)日：2012-10-04
申请号：US13072857
申请日：2011-03-28
申请人： Songping Gao
发明人： Songping Gao
IPC分类号： G01J1/58 , G01J3/44 , G01N21/00 , G01J3/30
CPC分类号： G01N21/65 , C12Q1/6869 , G01N21/6452 , C12Q2535/113 , C12Q2535/122 , C12Q2563/107 , C12Q2565/102 , C12Q2565/629 , C12Q2565/632
摘要： An optical detection system includes a detector configured to detect a signal emitted from a sample carrier and generate an output indicative of the signal detected by the detector. The sample carrier emits the signal in response to the sample carrier being scanned by an excitation source, the emitted signal is indicative of a structural characteristic of the sample carrier, and the sample carrier includes bulk material, at least one material free chamber and a bulk material/chamber interface for each chamber. The optical detection system further includes a data evaluator that identifies the structural characteristic of the sample carrier based on the output of the detector and generates data indicative of the identified structural characteristic.
摘要翻译：光学检测系统包括检测器，其被配置为检测从样本载体发射的信号，并产生指示由检测器检测到的信号的输出。样品载体响应于由激发源扫描的样品载体发射信号，所发射的信号指示样品载体的结构特征，并且样品载体包括散装材料，至少一个材料自由室和体积每个室的材料/室接口。光学检测系统还包括基于检测器的输出识别样品载体的结构特征的数据评估器，并产生指示所识别的结构特征的数据。

7. 发明申请

US20100171950A1 METHODS FOR UNIFORM METAL IMPREGNATION INTO A NANOPOROUS MATERIAL 有权
标题翻译：将金属均匀地纳入纳米材料的方法
公开(公告)号：US20100171950A1
公开(公告)日：2010-07-08
申请号：US12575369
申请日：2009-10-07
申请人： Selena CHAN , Sunghoon Kwon , Narayan Sundararajan
发明人： Selena CHAN , Sunghoon Kwon , Narayan Sundararajan
IPC分类号： G01J3/44
CPC分类号： G01N21/658 , B01D67/0062 , B01D67/0086 , B01D67/0088 , B01D71/02 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01J3/44 , G01N21/65 , G01N2021/6439 , G01N2021/655 , G01N2021/656 , C12Q2565/632 , C12Q2563/155
摘要： The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
摘要翻译：本文公开的方法，系统400和装置涉及金属150浸渍的多孔基材110,210。本发明的某些实施方案涉及用于制造金属涂覆的多孔硅基板110,210的方法，其显示出显着改善金属150沉积物的均匀性和深度的渗透。增加的均匀性和深度允许分析物的改进和更可重复的拉曼检测。在本发明的示例性实施例中，所述方法可以包括多孔硅110的氧化，浸入金属盐溶液130中，干燥和热分解金属盐140以形成金属沉积物150.在本发明的其它示例性实施方案中，方法可以包括多孔硅衬底210与一种或多种金属盐溶液130的微流体浸渍。本发明的其它实施方案涉及用于分析物的拉曼检测的装置和/或系统400，包括金属涂覆的多孔硅衬底110,210，由公开的方法。

8. 发明授权

US07738096B2 Surface enhanced Raman spectroscopy (SERS) systems, substrates, fabrication thereof, and methods of use thereof 失效
标题翻译：表面增强拉曼光谱（SERS）系统，基板，其制造及其使用方法
公开(公告)号：US07738096B2
公开(公告)日：2010-06-15
申请号：US11376661
申请日：2006-03-15
申请人： Yiping Zhao , Richard A. Dluhy , Ralph A. Tripp , Stephen Chaney , Saratchandra Shanmukh
发明人： Yiping Zhao , Richard A. Dluhy , Ralph A. Tripp , Stephen Chaney , Saratchandra Shanmukh
IPC分类号： G01J3/44
CPC分类号： G01N33/531 , C12Q1/6816 , C12Q1/6825 , G01N21/658 , G01N33/54373 , C12Q2565/632
摘要： Surface-enhanced Raman spectroscopic (SERS) systems including nanostructures and capable of detecting analytes, in particular biomolecules, of interest are provided. Methods of making the SERS systems and methods for detection of a biomolecule of interest, such as a virus or other infectious agent are also provided.
摘要翻译：提供了包括纳米结构和能够检测分析物，特别是感兴趣的生物分子的表面增强拉曼光谱（SERS）系统。还提供了制备SERS系统的方法和用于检测感兴趣的生物分子的方法，例如病毒或其它感染因子。

9. 发明申请

US20090298197A1 SERS-BASED METHODS FOR DETECTION OF BIOAGENTS 审中-公开
标题翻译：基于SERS的生物学检测方法
公开(公告)号：US20090298197A1
公开(公告)日：2009-12-03
申请号：US12093748
申请日：2006-11-15
申请人： Michael J. Natan , Michael Sha , William E. Doering
发明人： Michael J. Natan , Michael Sha , William E. Doering
IPC分类号： G01N33/566
CPC分类号： C12Q1/6818 , C12Q2565/632 , C12Q2565/549 , C12Q2565/1015
摘要： An assay and method of assay for optical detection of bioagents, a target nucleic acid or a target protein using a surface enhanced Raman scattering (SERS) active biomolecule molecular beacon. The present invention also provides the assay and method in a multiplexed format.
摘要翻译：使用表面增强拉曼散射（SERS）活性生物分子分子信标的生物标志物，靶核酸或靶蛋白的光学检测的测定和方法。本发明还提供了多种格式的测定方法。

10. 发明申请

US20090082220A1 Surface enhanced Raman spectroscopy (SERS) systems for the detection of bacteria and methods of use thereof 有权
标题翻译：用于细菌检测的表面增强拉曼光谱（SERS）系统及其使用方法
公开(公告)号：US20090082220A1
公开(公告)日：2009-03-26
申请号：US12214025
申请日：2008-06-16
申请人： Duncan C. Krause , Suzanne Marie Larkin Hennigan , Richard A. Dluhy , Jeremy Driskell , Yiping Zhao , Ralph A. Tripp
发明人： Duncan C. Krause , Suzanne Marie Larkin Hennigan , Richard A. Dluhy , Jeremy Driskell , Yiping Zhao , Ralph A. Tripp
IPC分类号： C40B30/10
CPC分类号： G01N33/56983 , B82Y5/00 , C12Q1/6816 , C12Q1/6825 , G01N21/658 , G01N33/531 , G01N33/54373 , G01N33/56911 , C12Q2565/632
摘要： Surface-enhanced Raman spectroscopic (SERS) systems and methods for detecting biomolecules of interest, such as a bacterium or virus are provided.
摘要翻译：提供表面增强拉曼光谱（SERS）系统和用于检测感兴趣的生物分子的方法，例如细菌或病毒。

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