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1. 发明授权

US09624539B2 DNA sequencing by synthesis using Raman and infrared spectroscopy detection 有权
公开(公告)号：US09624539B2
公开(公告)日：2017-04-18
申请号：US14119846
申请日：2012-05-23
申请人： Jingyue Ju , Jian Wu , Zengmin Li
发明人： Jingyue Ju , Jian Wu , Zengmin Li
IPC分类号： C12Q1/68 , C07H19/06 , C07H19/16 , C07H21/00
CPC分类号： C07H19/06 , C07H19/16 , C07H21/00 , C12Q1/6827 , C12Q1/6869 , C12Q1/6874 , C12Q2525/101 , C12Q2533/101 , C12Q2565/632
摘要： This invention provides nucleoside triphosphate analogs having the structure: wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is an OH or an H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1, and also to methods of DNA sequencing and SNP detection.

2. 发明授权

US09476094B2 Polynucleotide sequencing method 有权
标题翻译：多核苷酸测序方法
公开(公告)号：US09476094B2
公开(公告)日：2016-10-25
申请号：US10478036
申请日：2002-05-20
申请人： Daniel Henry Densham
发明人： Daniel Henry Densham
IPC分类号： C12Q1/00 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/632 , C12Q2533/101 , C12Q2521/543
摘要： The subject invention pertains to a method for determining the sequence of a polynucleotide comprising the steps of (i) contacting a polynucleotide processive enzyme immobilised in a fixed position, with a target polynucleotide under conditions sufficient to induce enzyme activity; (ii) detecting an effect consequent on the interaction of the enzyme and polynucleotide, wherein the effect is detected by measurement of a non-linear optical signal or a linear signal coupled to a non-linear signal.
摘要翻译：本发明涉及确定多核苷酸序列的方法，其包括以下步骤：（i）使固定位置的多核苷酸进程酶与靶多核苷酸在足以诱导酶活性的条件下接触; （ii）检测由酶和多核苷酸的相互作用产生的影响，其中通过测量非线性光信号或耦合到非线性信号的线性信号来检测效果。

3. 发明申请

US20160024570A1 RAMAN CLUSTER TAGGED MOLECULES FOR BIOLOGICAL IMAGING 审中-公开
标题翻译：用于生物成像的拉曼聚类标签分子
公开(公告)号：US20160024570A1
公开(公告)日：2016-01-28
申请号：US14775451
申请日：2014-03-14
申请人： Jingyue JU , Shiv KUMAR , Mirko PALLA , James J. RUSSO
发明人： Jingyue JU , Shiv KUMAR , Mirkó PALLA , James J. RUSSO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , G01N21/65 , G01N21/658 , C12Q2535/122 , C12Q2561/113 , C12Q2565/632
摘要： This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties.
摘要翻译：本发明提供核苷多磷酸酯类似物，其各自包含包含多个拉曼散射部分的标签; 包含所述核苷多磷酸酯类似物的化合物; 以及使用所述核苷多磷酸酯类似物测定单链DNA或RNA的序列的方法。本发明还提供了用于检测多个预定化合物的相互作用的方法，其中至少一个化合物与其附着有包含多个拉曼散射部分的标签。

4. 发明申请

US20150038347A1 SURFACE ENHANCED RAMAN SPECTROSCOPY 审中-公开
标题翻译：表面增强拉曼光谱
公开(公告)号：US20150038347A1
公开(公告)日：2015-02-05
申请号：US13635973
申请日：2011-03-21
申请人： Patrick A. Johnson , Hao Zhang , Mark Harpster
发明人： Patrick A. Johnson , Hao Zhang , Mark Harpster
IPC分类号： G01N21/65 , G01J3/44
CPC分类号： G01N21/658 , C12Q1/6825 , C12Q2563/143 , C12Q2565/632
摘要： Rapid surface enhanced Raman spectroscopy (SERS) assays for ultratrace pathogen detection are provided. One-particle and two-particle sensor assays (e.g., biosensor assays) are provided. In one implementation, for example, an assay forms and concentrates a DNA hybridization complex incorporating paramagnetic particles and Raman active noble metal (e.g., gold, silver and/or copper) nanoparticles (two-particle sensor) or noble metal coated paramagnetic particles that provide both a SERS substrate and magnetic capture ability in a single sensor particle via a noble metal-coated paramagnetic particle, such as a gold-coated paramagnetic particle (one-particle sensor).
摘要翻译：提供了用于超临界病原体检测的快速表面增强拉曼光谱（SERS）测定。提供单粒子和双粒子传感器测定（例如，生物传感器测定）。在一个实施方案中，例如，测定形成并浓缩包含顺磁性颗粒和拉曼活性贵金属（例如金，银和/或铜）纳米颗粒（双粒子传感器）或贵金属涂覆的顺磁性颗粒的DNA杂交复合物，其提供通过贵金属涂覆的顺磁性颗粒（例如金涂覆的顺磁性颗粒（单粒子传感器））在单个传感器颗粒中的SERS底物和磁捕获能力。

5. 发明授权

US08632975B2 Nucleotide transient binding for sequencing methods 有权
标题翻译：测序方法的核苷酸瞬变结合
公开(公告)号：US08632975B2
公开(公告)日：2014-01-21
申请号：US12790760
申请日：2010-05-28
申请人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
发明人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

6. 发明授权

US08427637B2 Optical detection system 有权
标题翻译：光学检测系统
公开(公告)号：US08427637B2
公开(公告)日：2013-04-23
申请号：US13072857
申请日：2011-03-28
申请人： Songping Gao
发明人： Songping Gao
IPC分类号： G01N1/10
CPC分类号： G01N21/65 , C12Q1/6869 , G01N21/6452 , C12Q2535/113 , C12Q2535/122 , C12Q2563/107 , C12Q2565/102 , C12Q2565/629 , C12Q2565/632
摘要： An optical detection system includes a detector configured to detect a signal emitted from a sample carrier and generate an output indicative of the signal detected by the detector. The sample carrier emits the signal in response to the sample carrier being scanned by an excitation source, the emitted signal is indicative of a structural characteristic of the sample carrier, and the sample carrier includes bulk material, at least one material free chamber and a bulk material/chamber interface for each chamber. The optical detection system further includes a data evaluator that identifies the structural characteristic of the sample carrier based on the output of the detector and generates data indicative of the identified structural characteristic.
摘要翻译：光学检测系统包括检测器，其被配置为检测从样本载体发射的信号，并产生指示由检测器检测到的信号的输出。样品载体响应于由激发源扫描的样品载体发射信号，所发射的信号指示样品载体的结构特征，并且样品载体包括散装材料，至少一个材料自由室和体积每个室的材料/室接口。光学检测系统还包括基于检测器的输出识别样品载体的结构特征的数据评估器，并产生指示所识别的结构特征的数据。

7. 发明授权

US08227590B2 High sensitivity assay for molecular typing of biological sample, probes and a kit thereof 失效
标题翻译：用于生物样品，探针及其试剂盒的分子分型的高灵敏度测定
公开(公告)号：US08227590B2
公开(公告)日：2012-07-24
申请号：US12515766
申请日：2007-11-19
申请人： Udaykumar Ranga , Chandrabhas Narayana , Jayasuryan Narayana
发明人： Udaykumar Ranga , Chandrabhas Narayana , Jayasuryan Narayana
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/703 , C12Q2565/632 , C12Q2565/519 , C12Q2537/125
摘要： The present invention relates to a high sensitivity assay for molecular typing of a biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); capture probes for capturing nucleic acid; a detector probe to detect captured nucleic acid; a kit for molecular typing of biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); and lastly a method of manufacturing said kit.
摘要翻译：本发明涉及使用包括共振散射（SERDMA）的表面增强拉曼散射（SERS）的生物样品的分子分型的高灵敏度测定; 用于捕获核酸的捕获探针; 用于检测捕获的核酸的检测器探针; 使用包括共振散射（SERDMA）的表面增强拉曼散射（SERS）的生物样品的分子分型试剂盒; 最后是制造所述试剂盒的方法。

8. 发明申请

US20120164624A1 SERS Nanotag Assays 审中-公开
标题翻译： SERS Nanotag测定
公开(公告)号：US20120164624A1
公开(公告)日：2012-06-28
申请号：US12184691
申请日：2008-08-01
申请人： Michael J. Natan , Sharron Gaynor Penn , Remy Cromer , Michael Sha
发明人： Michael J. Natan , Sharron Gaynor Penn , Remy Cromer , Michael Sha
IPC分类号： G01N21/65 , B82Y15/00
CPC分类号： G01N21/658 , B82Y5/00 , C12Q1/6816 , G01N33/54373 , Y10T436/143333 , C12Q2537/125 , C12Q2563/131 , C12Q2563/143 , C12Q2565/632
摘要： Methods and systems for the use of Surface Enhanced Raman Scattering nanotags (SERS nanotags) to create homogeneous (no-wash), heterogeneous or sequence detection assay platforms. In certain embodiments the SERS nanotags are used in combination with magnetic particles. Multiplexed assay platforms are also disclosed. In certain embodiments, the assay is useful for clinical proteomics. Assay platforms suitable for use within a biological matrix, for example within whole blood or serum are also disclosed. The assay formats described herein may be used to detect any analyte of interest including but not limited to the detection of cells, viruses, bacteria, proteins, DNA, RNA, or small molecules in any type of biological (animal or plant kingdom) or environmental samples including but not limited to whole blood or serum, occult samples, urine, feces, air, drinking water, phage, any organism, multicellular clumps of cells, for example, cancer tissue homogenate.
摘要翻译：使用表面增强拉曼散射纳米标签（SERS纳米标签）的方法和系统，以创建均质（非洗涤），异质或序列检测测定平台。在某些实施方案中，SERS纳米颗粒与磁性颗粒结合使用。还公开了多重测定平台。在某些实施方案中，该测定可用于临床蛋白质组学。还公开了适用于生物基质，例如全血或血清中的测定平台。本文描述的测定形式可用于检测感兴趣的任何分析物，包括但不限于检测任何类型的生物（动物或植物界）或环境中的细胞，病毒，细菌，蛋白质，DNA，RNA或小分子样品包括但不限于全血或血清，隐匿样品，尿液，粪便，空气，饮用水，噬菌体，任何生物，细胞多细胞团，例如癌组织匀浆。

9. 发明申请

US20100285468A1 DETECTION AND/OR QUANTIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：检测和/或定量核酸
公开(公告)号：US20100285468A1
公开(公告)日：2010-11-11
申请号：US12679534
申请日：2008-09-24
申请人： Xing Xin
发明人： Xing Xin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156 , C12Q2565/632 , C12Q2563/173 , C12Q2545/101 , C12Q2531/113 , C12Q2527/107
摘要： The present invention provides compositions, methods, and kits for nucleic acids analyses. In particular, melting analyses are used to detect the presence or absence and to quantify nucleic acids.
摘要翻译：本发明提供了用于核酸分析的组合物，方法和试剂盒。特别地，使用熔解分析来检测核酸的存在或不存在以及定量。

10. 发明申请

US20100151454A1 MICROFLUIDIC APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING MOLECULAR REACTIONS 审中-公开
标题翻译：用于实施分子反应的微流感设备，系统和方法
公开(公告)号：US20100151454A1
公开(公告)日：2010-06-17
申请号：US12256409
申请日：2008-10-22
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

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