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1. 发明申请

US20100151454A1 MICROFLUIDIC APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING MOLECULAR REACTIONS 审中-公开
标题翻译：用于实施分子反应的微流感设备，系统和方法
公开(公告)号：US20100151454A1
公开(公告)日：2010-06-17
申请号：US12256409
申请日：2008-10-22
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

2. 发明授权

US07442339B2 Microfluidic apparatus, Raman spectroscopy systems, and methods for performing molecular reactions 有权
标题翻译：微流体装置，拉曼光谱系统和进行分子反应的方法
公开(公告)号：US07442339B2
公开(公告)日：2008-10-28
申请号：US10815264
申请日：2004-03-31
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew A. Berlin
IPC分类号： G01N21/65 , G01J3/44 , G01N33/48
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

3. 发明申请

US20050221333A1 Microfluidic apparatus, systems, and methods for performing molecular reactions 有权
标题翻译：用于进行分子反应的微流体装置，系统和方法
公开(公告)号：US20050221333A1
公开(公告)日：2005-10-06
申请号：US10815264
申请日：2004-03-31
申请人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew Berlin
发明人： Narayanan Sundararajan , Lei Sun , Yuegang Zhang , Xing Su , Selena Chan , Tae-Woong Koo , Andrew Berlin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G01N21/658 , Y10T436/11 , Y10T436/117497 , Y10T436/143333 , C12Q2565/629 , C12Q2521/319 , C12Q2565/632 , C12Q2565/619
摘要： Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
摘要翻译：本文公开了用于在微流体通道中进行核酸测序反应和分子结合反应的方法，装置和系统。方法，装置和系统可以包括限制核酸附着的颗粒运动的限制障碍。此外，方法，装置和系统可以包括输送流的流体动力聚焦。此外，方法，装置和系统可以通过在表面和输送流之间提供保护流来减少与微流体通道的表面的非特异性相互作用。

4. 发明申请

US20050148098A1 Methods for using raman spectroscopy to obtain a protein profile of a biological sample 审中-公开
标题翻译：使用拉曼光谱法获得生物样品的蛋白质谱的方法
公开(公告)号：US20050148098A1
公开(公告)日：2005-07-07
申请号：US10749528
申请日：2003-12-30
申请人： Xing Su , Lei Sun , Mineo Yamakawa , Tae-Woong Koo , Selena Chan , Andrew Berlin , Narayanan Sundararajan
发明人： Xing Su , Lei Sun , Mineo Yamakawa , Tae-Woong Koo , Selena Chan , Andrew Berlin , Narayanan Sundararajan
IPC分类号： G01N21/65 , G01N33/58 , G01N33/68 , G06F19/00 , G01N33/48 , G01N33/50 , G01N33/543 , G01N33/553
CPC分类号： G01N21/658 , G01N33/6803 , G01N2021/653 , G01N2021/655 , G01N2021/656
摘要： The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.
摘要翻译：本发明提供了用于分析生物样品的蛋白质含量的方法，例如获得由特定个体提供的样品的蛋白质谱。样品中的蛋白质和蛋白质片段基于化学和/或物理性质分离，并在固体基质上或流动液体流中的离散位置保持分离状态。然后检测拉曼光谱，由离散位置处的分离的蛋白质或片段产生，使得离散位置的光谱提供关于在离散位置处的一种或多种特定蛋白质或片段的结构或身份的信息。离散位置处的蛋白质或片段可以用金属（例如金或银）涂覆，和/或分离的蛋白质可与化学增强剂接触以提供SERS光谱。还提供了用于实施本发明的方法和试剂盒。

5. 发明申请

US20050250159A1 Methods for using Raman spectroscopy to obtain a protein profile of a biological sample 审中-公开
标题翻译：使用拉曼光谱法获得生物样品的蛋白质谱的方法
公开(公告)号：US20050250159A1
公开(公告)日：2005-11-10
申请号：US11083418
申请日：2005-03-17
申请人： Xing Su , Lei Sun , Mineo Yamakawa , Tae-Woong Koo , Selena Chan , Andrew Berlin , Narayanan Sundararajan
发明人： Xing Su , Lei Sun , Mineo Yamakawa , Tae-Woong Koo , Selena Chan , Andrew Berlin , Narayanan Sundararajan
IPC分类号： G01N21/65 , G01N33/58 , G01N33/68 , G01N33/53 , G01N33/00
CPC分类号： G01N21/658 , G01N33/6803 , G01N2021/653 , G01N2021/655 , G01N2021/656
摘要： The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.
摘要翻译：本发明提供了用于分析生物样品的蛋白质含量的方法，例如获得由特定个体提供的样品的蛋白质谱。样品中的蛋白质和蛋白质片段基于化学和/或物理性质分离，并在固体基质上或流动液体流中的离散位置保持分离状态。然后检测拉曼光谱，由离散位置处的分离的蛋白质或片段产生，使得离散位置的光谱提供关于在离散位置处的一种或多种特定蛋白质或片段的结构或身份的信息。离散位置处的蛋白质或片段可以用金属（例如金或银）涂覆，和/或分离的蛋白质可与化学增强剂接触以提供SERS光谱。还提供了用于实施本发明的方法和试剂盒。

6. 发明申请

US20050202468A1 Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication 审中-公开
标题翻译：通过拉曼监测分子复制过程中核苷酸摄取的核酸测序
公开(公告)号：US20050202468A1
公开(公告)日：2005-09-15
申请号：US11020776
申请日：2004-12-23
申请人： Tae-Woong Koo , Andrew Berlin , Selena Chan , Xing Su , Narayanan Sundararajan , Lei Sun
发明人： Tae-Woong Koo , Andrew Berlin , Selena Chan , Xing Su , Narayanan Sundararajan , Lei Sun
IPC分类号： C12Q1/68 , G01N21/65
CPC分类号： G01N21/658 , C12Q1/6869 , C12Q2565/632 , C12Q2533/101
摘要： The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.
摘要翻译：本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。合成的新生链的核酸序列和模板链的互补序列可以通过跟踪在聚合反应期间核苷酸掺入的顺序来确定。提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。此外，提供了用于检测单碱基重复的方法。

7. 发明申请

US20050147979A1 Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication 审中-公开
公开(公告)号：US20050147979A1
公开(公告)日：2005-07-07
申请号：US10749527
申请日：2003-12-30
申请人： Tae-Woong Koo , Andrew Berlin , Selena Chan , Xing Su , Narayanan Sundararajan , Lei Sun
发明人： Tae-Woong Koo , Andrew Berlin , Selena Chan , Xing Su , Narayanan Sundararajan , Lei Sun
IPC分类号： C12Q1/68 , G01N21/65 , C12P19/34
CPC分类号： G01N21/658 , C12Q1/6869 , C12Q2565/632 , C12Q2533/101
摘要： The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

8. 发明申请

US20090169466A1 Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
公开(公告)号：US20090169466A1
公开(公告)日：2009-07-02
申请号：US11344712
申请日：2006-01-31
申请人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
发明人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
IPC分类号： D01F9/12 , C07H21/04
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

9. 发明申请

US20090170725A1 Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning 审中-公开
标题翻译：使用肽或核酸微图案生产碳纳米管的方法
公开(公告)号：US20090170725A1
公开(公告)日：2009-07-02
申请号：US11344713
申请日：2006-01-31
申请人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
发明人： Mineo Yamakawa , Yuegang Zhang , Xing Su , Lei Sun , Andrew A. Berlin , Narayanan Sundararajan
IPC分类号： C40B40/08 , B82B1/00
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles 140, 230 to polymer 120, 210 molecules, attaching the polymer 120, 210 molecules to a substrate, removing the polymer 120, 210 molecules and producing carbon nanotubes on the catalyst nanoparticles 140, 230. The polymer 120, 210 molecules can be attached to the substrate in ordered patterns, using self-assembly or molecular alignment techniques. The nanotube arrays can be attached to selected areas 110, 310 of the substrate. Within the selected areas 110, 310, the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.
摘要翻译：本文公开的方法，装置和系统涉及碳纳米管的有序阵列。在本发明的具体实施方案中，纳米管阵列通过包括将催化剂纳米颗粒140,230连接到聚合物120,210分子，将聚合物120,210分子连接到基底上的方法形成，除去聚合物120,210分子并产生碳催化剂纳米颗粒140,230上的纳米管。聚合物120,210分子可以使用自组装或分子对准技术以有序图案附着到基底上。纳米管阵列可以附着到基板的选定区域110,310。在所选择的区域110,310内，纳米管是非随机分布的。本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列和包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统的装置。在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

10. 发明申请

US20070054288A1 Programmable molecular barcodes 审中-公开
标题翻译：可编程分子条形码
公开(公告)号：US20070054288A1
公开(公告)日：2007-03-08
申请号：US11430612
申请日：2006-05-08
申请人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
IPC分类号： C12Q1/68 , C12M1/34 , G06K7/10
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.
摘要翻译：本公开涉及用于生产和/或使用分子条形码的方法。在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。标签可以附加到骨干和/或分支结构。条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。可以通过标签的类型和位置区分不同的条形码。在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。在替代实施例中，条形码可以通过单体单元的聚合来制备。可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

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