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1. 发明申请

US20070099211A1 Detection of nucleic acid amplification 审中-公开
标题翻译：检测核酸扩增
公开(公告)号：US20070099211A1
公开(公告)日：2007-05-03
申请号：US11488439
申请日：2006-07-17
申请人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
发明人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6823 , B01L3/5027 , B01L7/525 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/1844 , B01L2300/185 , B01L2300/1872 , B01L2300/1894 , B01L2400/0418 , C12Q1/6825 , C12Q2565/607 , C12Q2563/131 , C12Q2565/629 , C12Q2531/113 , C12Q2521/301 , C12Q2523/107
摘要： Methods for detecting a target polynucleotide sequences are provided that utilize a probe having a target-complementary segment and a detectable tag. By cleaving the detectable tab and associating the tag with a tag complement coupled to an electrode, an electrochemical signal can be detected that is related to the presence of the tag:tag complement complex.
摘要翻译：提供了检测靶多核苷酸序列的方法，其利用具有靶互补区段和可检测标签的探针。通过切割可检测的标签并将标签与耦合到电极的标签补体相关联，可以检测到与标签：标签补体复合物的存在相关的电化学信号。

2. 发明申请

US20080241838A1 METHODS AND SYSTEMS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：检测核酸的方法和系统
公开(公告)号：US20080241838A1
公开(公告)日：2008-10-02
申请号：US11965837
申请日：2007-12-28
申请人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
发明人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。这些捕获探针比完整的杂交探针更容易与探针片段结合。然后可以在支撑表面上检测标签。以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

3. 发明申请

US20110014617A1 Methods and Systems for Detecting Nucleic Acids 审中-公开
标题翻译：检测核酸的方法和系统
公开(公告)号：US20110014617A1
公开(公告)日：2011-01-20
申请号：US12816006
申请日：2010-06-15
申请人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
发明人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。这些捕获探针比完整的杂交探针更容易与探针片段结合。然后可以在支撑表面上检测标签。以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

4. 发明申请

US20070175768A1 MICROFLUIDIC SYSTEMS INCLUDING POROUS POLYMER ELECTRODES 审中-公开
标题翻译：包含多孔聚合物电极的微流体系统
公开(公告)号：US20070175768A1
公开(公告)日：2007-08-02
申请号：US11558343
申请日：2006-11-09
申请人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
发明人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
IPC分类号： C12Q1/00 , G01N27/26 , G01N33/487 , G01F1/64
CPC分类号： G01N27/44704 , B01L3/5023 , B01L3/502707 , B01L3/502753 , B01L7/52 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2400/0418 , B01L2400/0421
摘要： Microfluidic devices that incorporate a porous polymer electrode assemblies, including microfluidic device useful for detection of nucleic acids, as well as methods of using the microfluidic devices.
摘要翻译：包含多孔聚合物电极组件的微流体装置，包括可用于检测核酸的微流体装置，以及使用微流体装置的方法。

5. 发明申请

US20070114128A1 Porous polymer electrodes 审中-公开
标题翻译：多孔聚合物电极
公开(公告)号：US20070114128A1
公开(公告)日：2007-05-24
申请号：US11479175
申请日：2006-06-30
申请人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
发明人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
IPC分类号： C25C7/02 , C25B11/03
CPC分类号： G01N27/3335
摘要： Porous polymer electrode assemblies are useful in the detection or quantification of a variety of analytes. By preparing a porous monolith, and applying a conductive polymer to the monolith, a porous matrix is prepared that combines favorable conductive properties, by virtue of the presence of the conductive polymer, with the porous character of the underlying monolith. The resulting porous electrode can be used for qualitative or quantitative analysis, and the capture and/or release of selected charged materials, such as nucleic acids. The pores of the electrode matrix may also be filled with nonconductive material, yielding electrodes having a plurality of discrete conductive surfaces.
摘要翻译：多孔聚合物电极组件可用于检测或定量各种分析物。通过制备多孔整料并将导电聚合物施加到整料上，制备出多孔基质，其通过导电聚合物的存在与下面的整料的多孔特征结合有利的导电性能。所得的多孔电极可用于定性或定量分析，以及所选择的带电材料（例如核酸）的捕获和/或释放。电极基体的孔也可以用非导电材料填充，产生具有多个离散导电表面的电极。

6. 发明申请

US20070212695A1 Compositions, methods, and kits for selective amplification of nucleic acids 审中-公开
标题翻译：用于选择性扩增核酸的组合物，方法和试剂盒
公开(公告)号：US20070212695A1
公开(公告)日：2007-09-13
申请号：US11331599
申请日：2006-01-12
申请人： Vissarion Aivazachvili , Konrad Faulstich , Tony Tran
发明人： Vissarion Aivazachvili , Konrad Faulstich , Tony Tran
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , Y02A50/54 , C12Q2525/307 , C12Q2531/125 , C12Q2525/117 , C12Q2561/125 , C12Q2537/143
摘要： The current teachings are directed to compositions, methods, and kits for selectively amplifying and for detecting target sequences. In some embodiments, a circularizable probe and/or a probe pair are disclosed for selectively amplifying target sequences. Methods for selectively amplifying target sequences are also disclosed, as are methods for detecting selectively amplified target sequences. Certain embodiments of the disclosed methods comprise a circularizable probe, a probe pair, comprising a first probe and a second probe, or both. In certain embodiments, a multiplicity of different circularizable probes, a multiplicity of different probe sets, or a multiplicity of different circularizable probes and a multiplicity of different probe sets are provided to selectively amplify or to detect a multiplicity of different target sequences, typically in a multiplex reaction. According to certain disclosed methods, surrogates of the target sequences are selectively amplified, including without limitation ligated probes, first amplification products, second amplification products, or combinations thereof. In some embodiments, selectively amplified target sequences or their surrogates are detected, directly or indirectly, indicating the presence of the corresponding target sequence. Kits to facilitate the performance of the disclosed methods are also provided.
摘要翻译：目前的教导涉及用于选择性扩增和检测靶序列的组合物，方法和试剂盒。在一些实施方案中，公开了可圆化探针和/或探针对，用于选择性扩增靶序列。还公开了选择性扩增靶序列的方法，以及用于检测选择性扩增的靶序列的方法。所公开方法的某些实施方案包括可圆化的探针，包括第一探针和第二探针的探针对，或两者。在某些实施方案中，提供多个不同的可圆化探针，多个不同的探针组或多个不同的可环化探针和多个不同的探针组，以选择性地扩增或检测多种不同靶序列，通常在多重反应。根据某些公开的方法，选择性扩增靶序列的替代物，包括但不限于连接的探针，第一扩增产物，第二扩增产物或其组合。在一些实施方案中，直接或间接检测选择性扩增的靶序列或其替代物，表明存在相应的靶序列。还提供了用于促进所公开方法的性能的套件。

7. 发明申请

US20060166347A1 Sample preparation devices and methods 审中-公开
标题翻译：样品制备装置和方法
公开(公告)号：US20060166347A1
公开(公告)日：2006-07-27
申请号：US11342012
申请日：2006-01-27
申请人： Konrad Faulstich , John Van Camp , Charles Vann
发明人： Konrad Faulstich , John Van Camp , Charles Vann
IPC分类号： C12N1/06
CPC分类号： C12N1/066 , B01L3/502 , B01L2300/0681 , B01L2400/0478 , B01L2400/0605 , C12N1/06 , G01N2001/4088
摘要： A method and device for preparing biological samples includes lysing the microorganisms, and collecting a desired biological material.
摘要翻译：用于制备生物样品的方法和装置包括裂解微生物并收集所需的生物材料。

8. 发明授权

US08147770B2 Microfluidic device including a dissolvable structure for flow control 有权
标题翻译：微流体装置包括用于流量控制的可溶解结构
公开(公告)号：US08147770B2
公开(公告)日：2012-04-03
申请号：US12954519
申请日：2010-11-24
申请人： Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Umberto Ulmanella , Jun Xie
发明人： Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Umberto Ulmanella , Jun Xie
IPC分类号： B01D11/04 , B01L3/00 , B01L3/02 , G01N1/10
CPC分类号： F16K99/0001 , B01L3/502738 , B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0661 , B01L2400/0677 , B01L2400/0683 , B01L2400/082 , B01L2400/084 , B01L2400/086 , F16K99/003 , F16K99/0036 , F16K99/0057 , F16K2099/0084 , Y10S422/901 , Y10T137/0318 , Y10T137/0324 , Y10T137/1624 , Y10T137/1812 , Y10T137/2191 , Y10T137/8376 , Y10T137/8593 , Y10T137/85978 , Y10T436/143333 , Y10T436/2575
摘要： A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.
摘要翻译：提供了一种诊断装置，其包括多个保持区域，其中保留区域被至少一个可溶解屏障隔开。保持区域可以通过至少一个流体处理通道相互连接。保持区域可以包括诸如保持区域，孔，腔室或其它容器的容器，或诸如保持材料的表面的保持区域。保留区域可以包括反应保留区域，一个或多个试剂保留区域，每个含有未反应的试剂和样品保留区域。压力致动阀可以定位在每个流体处理通道中，其将一个或多个试剂保留区域与插入在一个或多个试剂保留区域中的每一个和反应保留区域之间的各个中间保留区域互连。可溶解屏障可以是至少一个流体处理通道中的流体流动调节器。

9. 发明授权

US08383062B2 Fluid processing device including size-changing barrier 有权
标题翻译：流体处理装置包括尺寸变化的屏障
公开(公告)号：US08383062B2
公开(公告)日：2013-02-26
申请号：US12945793
申请日：2010-11-12
申请人： Umberto Ulmanella , Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Jun Xie
发明人： Umberto Ulmanella , Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Jun Xie
IPC分类号： B01L3/00 , C12Q1/68 , G01N1/10
CPC分类号： F16K99/0001 , B01L3/502738 , B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0661 , B01L2400/0677 , B01L2400/0683 , B01L2400/082 , B01L2400/084 , B01L2400/086 , F16K99/003 , F16K99/0036 , F16K99/0057 , F16K2099/0084 , Y10S422/901 , Y10T137/0318 , Y10T137/0324 , Y10T137/1624 , Y10T137/1812 , Y10T137/2191 , Y10T137/8376 , Y10T137/8593 , Y10T137/85978 , Y10T436/143333 , Y10T436/2575
摘要： A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.
摘要翻译：提供了诊断装置，其包括通过至少一个流体处理通道互连或由至少一个屏障隔开的多个保持区域。如果提供流体处理通道，流体流动调节器可以设置在流体处理通道中。阻隔和/或流体流动调节器可以包含多糖，多糖的衍生物或其组合。例如，阻挡层可以包含壳聚糖材料。

10. 发明申请

US20060090800A1 Fluid processing device including size-changing barrier 审中-公开
公开(公告)号：US20060090800A1
公开(公告)日：2006-05-04
申请号：US11252912
申请日：2005-10-18
申请人： Debjyoti Banerjee , Umberto Ulmanella , Konrad Faulstich , Aldrich Lau , Jun Xie
发明人： Debjyoti Banerjee , Umberto Ulmanella , Konrad Faulstich , Aldrich Lau , Jun Xie
IPC分类号： F15C1/04
CPC分类号： F16K99/0001 , B01L3/502738 , B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0661 , B01L2400/0677 , B01L2400/0683 , B01L2400/082 , B01L2400/084 , B01L2400/086 , F16K99/003 , F16K99/0036 , F16K99/0057 , F16K2099/0084 , Y10S422/901 , Y10T137/0318 , Y10T137/0324 , Y10T137/1624 , Y10T137/1812 , Y10T137/2191 , Y10T137/8376 , Y10T137/8593 , Y10T137/85978 , Y10T436/143333 , Y10T436/2575
摘要： A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.

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