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1. 发明授权

US12110540B2 Compositions and methods for detecting toxigenic 有权
公开(公告)号：US12110540B2
公开(公告)日：2024-10-08
申请号：US16769229
申请日：2018-12-13
申请人： Gen-Probe Incorporated
发明人： Patrick Lynn Peterson
IPC分类号： C12Q1/689 , C12Q1/6827
CPC分类号： C12Q1/6827 , C12Q1/689 , C12Q2537/143 , C12Q2561/109 , C12Q2565/101 , C12Q2600/156 , C12Q2600/166
摘要： Provided herein are compositions, kits, and methods for detecting at least one of a C. diffcile tcdA, tcdB, tcdC, cdtA, or cdtB nucleic acid in a sample. In son embodiments, one or more alleles of tcdC such as 117del tcdC or 184T tcdC are detected.

2. 发明授权

US12060620B2 Multiplexed KRAS mutation detection assay 有权
公开(公告)号：US12060620B2
公开(公告)日：2024-08-13
申请号：US17739892
申请日：2022-05-09
申请人： Exact Sciences Corporation
发明人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
IPC分类号： C12Q1/68 , C12Q1/6858 , C12Q1/6886
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q1/6858 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

3. 发明授权

US10626465B2 Multiplexed KRAS mutation detection assay 有权
公开(公告)号：US10626465B2
公开(公告)日：2020-04-21
申请号：US16113892
申请日：2018-08-27
申请人： Exact Sciences Development Company, LLC
发明人： Rebecca Oldham-Haltom , Hatim Allawi , Hongzhi Zou , Michael J. Domanico , Graham P. Lidgard
IPC分类号： C12Q1/68 , C12Q1/6886 , C12Q1/6858
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , C12Q1/6858 , C12Q2525/161 , C12Q2561/109 , C12Q2565/1015
摘要： Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

4. 发明申请

US20190218601A1 METHYLATED CONTROL DNA 审中-公开
公开(公告)号：US20190218601A1
公开(公告)日：2019-07-18
申请号：US16318580
申请日：2017-07-19
申请人： Exact Sciences Development Company, LLC
发明人： Hatim T. Allawi , Graham P. Lidgard
IPC分类号： C12Q1/6827
CPC分类号： C12Q1/6827 , C12Q2523/125 , C12Q2531/113 , C12Q2545/101 , C12Q2561/109 , C12Q2600/154
摘要： Provided herein is technology relating compositions and methods for analysis of methylated DNA from a subject. The technology also relates to use of endogenous methylated DNAs as internal controls for marker gene methylation assays.

5. 发明申请

US20180057869A1 DETECTION OF TARGET NUCLEIC ACID SEQUENCE BY PTO CLEAVAGE AND EXTENSION-DEPENDENT NON-HYBRIDIZATION ASSAY 审中-公开
公开(公告)号：US20180057869A1
公开(公告)日：2018-03-01
申请号：US15697617
申请日：2017-09-07
申请人： SEEGENE, INC.
发明人： Jong Yoon CHUN , Young Jo LEE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6823 , C12Q1/6834 , C12Q1/6851 , C12Q2527/107 , C12Q2561/109 , C12Q2565/1015 , C12Q2561/113 , C12Q2525/161 , C12Q2561/101 , C12Q2521/319 , C12Q2525/121 , C12Q2537/149 , C12Q2565/101 , C12Q2565/501
摘要： The present invention relates to the detection of a target nucleic acid sequence by a PCE-NH (PTO Cleavage and Extension-Dependent Non-Hybridization) assay. The present invention adopts the occurrence of the inhibition of the hybridization between the HO with the CTO by the formation of the target-dependent extended duplex. Therefore, the present invention may detect target sequences even when the HO is not cleaved. In this regard, the design of the 5′-tagging portion of PTO, CTO and HO sequences may be readily performed and the conditions for reactions may be also easily established. In addition, the detection of the hybrid between the CTO and the HO may be performed in a different vessel from that for the extension of the CTO.

6. 发明授权

US09840739B2 Method for determining SNP genotype 有权
公开(公告)号：US09840739B2
公开(公告)日：2017-12-12
申请号：US14908329
申请日：2014-07-30
申请人： SEEGENE, INC.
发明人： Jong Yoon Chun , Young Jo Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/107 , C12Q2533/101 , C12Q2561/109 , C12Q2565/1015
摘要： The present invention is generally drawn to a novel method for determining a SNP (single nucleotide polymorphism) genotype using a PTO-SNV (Probing and Tagging Oligonucleotide for Single Nucleotide Variation). The present invention provides novel protocols for SNP genotyping in which only one allele-specific oligonucleotide permits in a SNP genotyping reaction to determine whether a target nucleic acid sequence to be analyzed is homozygous or heterozygous for the SNP allele of interest or has no SNP allele of interest.

7. 发明授权

US09290797B2 Real time cleavage assay 有权
公开(公告)号：US09290797B2
公开(公告)日：2016-03-22
申请号：US13720757
申请日：2012-12-19
申请人： Exact Sciences Corporation
发明人： Rebecca Oldham-Haltom , Hongzhi Zou , Graham P. Lidgard , Michael J. Domanico , Hatim Allawi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/686 , C12Q1/6886 , C12Q2600/156 , C12Q2600/158 , C12Q2525/301 , C12Q2527/101 , C12Q2561/109 , C12Q2565/1015
摘要： A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

8. 发明申请

US20160077041A1 ELECTROCHEMICAL DETECTION SYSTEM AND APPARATUS 审中-公开
标题翻译：电化学检测系统和装置
公开(公告)号：US20160077041A1
公开(公告)日：2016-03-17
申请号：US14845686
申请日：2015-09-04
申请人： ATLAS GENETICS LIMITED
发明人： Helen Braven , Russell Keay
IPC分类号： G01N27/327 , G01N27/30 , G01N27/416 , B01L7/00
CPC分类号： G01N27/3277 , B01L7/52 , B01L2300/0645 , B01L2300/18 , C12Q1/6816 , C12Q1/6823 , C12Q1/686 , G01N27/308 , G01N27/416 , G01N33/48721 , G01N2458/30 , C12Q2561/101 , C12Q2561/109 , C12Q2563/113 , C12Q2565/107
摘要： The invention provides a method of probing for a nucleic acid comprising: contacting a nucleic acid solution with an oligonucleotide probe labelled with an electrochemically active marker, providing conditions at which the probe is able to at least partially hybridise with any complementary target sequence which may be present in the nucleic acid solution, selectively degrading either hybridised, partially hybridised or unhybridised nucleic acid probe, and electrochemically determining information relating to the electrochemically active marker. The invention further provides novel molecules with use in methods of the invention.
摘要翻译：本发明提供了一种探测核酸的方法，包括：使核酸溶液与用电化学活性标记物标记的寡核苷酸探针接触，提供探针能够与任何互补靶序列至少部分杂交的条件，所述互补靶序列可以是存在于核酸溶液中，选择性降解杂交的，部分杂交的或未杂交的核酸探针，以及电化学测定与电化学活性标记相关的信息。本发明还提供了用于本发明方法的新型分子。

9. 发明申请

US20160025703A1 Nucleic Acid Probes, their Synthesis and Use 审中-公开
标题翻译：核酸探针，其合成与应用
公开(公告)号：US20160025703A1
公开(公告)日：2016-01-28
申请号：US14818930
申请日：2015-08-05
申请人： Atlas Genetics Limited
发明人： Helen Braven , Russell Keay
IPC分类号： G01N33/487 , C12Q1/68
CPC分类号： G01N27/3277 , B01L7/52 , B01L2300/0645 , B01L2300/18 , C12Q1/6816 , C12Q1/6823 , C12Q1/686 , G01N27/308 , G01N27/416 , G01N33/48721 , G01N2458/30 , C12Q2561/101 , C12Q2561/109 , C12Q2563/113 , C12Q2565/107
摘要： The invention provides a method of probing for a nucleic acid comprising: contacting a nucleic acid solution with an oligonucleotide probe labelled with an electrochemically active marker, providing conditions at which the probe is able to at least partially hybridise with any complementary target sequence which may be present in the nucleic acid solution, selectively degrading either hybridised, partially hybridised or unhybridised nucleic acid probe, and electrochemically determining information relating to the electrochemically active marker. The invention further provides novel molecules with use in methods of the invention.
摘要翻译：本发明提供了一种探测核酸的方法，包括：使核酸溶液与用电化学活性标记物标记的寡核苷酸探针接触，提供探针能够与任何互补靶序列至少部分杂交的条件，所述互补靶序列可以是存在于核酸溶液中，选择性降解杂交的，部分杂交的或未杂交的核酸探针，以及电化学测定与电化学活性标记相关的信息。本发明还提供了用于本发明方法的新型分子。

10. 发明授权

US09121056B2 Use of products of PCR amplification carrying elements of secondary structure to improve PCR-based nucleic acid detection 有权
标题翻译：使用携带二级结构元件的PCR扩增产物来改进基于PCR的核酸检测
公开(公告)号：US09121056B2
公开(公告)日：2015-09-01
申请号：US12298900
申请日：2007-04-30
申请人： Igor Kutyavin
发明人： Igor Kutyavin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/686 , C12Q2561/109 , C12Q2537/143 , C12Q2525/301 , C12Q2565/1015
摘要： Particular aspects comprise amplifying target nucleic acid using PCR and an oligonucleotide primer pair wherein at least one of the primers is designed to incorporate a 5′-specialty sequence to provide for an amplification product that intramolecularly folds into a secondary structure; and detecting the amplification product by a method comprising: providing an oligonucleotide cleavage component, hybridizing the oligonucleotide cleavage component with the amplification product to form a three-strand cleavage structure wherein two strands of the three-strand cleavage structure are provided by the secondary structure of the amplification product, cleaving 3′- or 5′-strands of the three-strand cleavage structure using a duplex-specific nuclease activity resulting in a cleavage product, and detecting the cleavage product indicative of the presence of the target nucleic acid. In certain aspects both primers incorporate a 5′-specialty sequence and detecting comprises cleaving 3′- or 5′-strands of a three-strand cleavage structure using duplex-specific nuclease to provide a cleavage product.
摘要翻译：具体方面包括使用PCR扩增靶核酸和寡核苷酸引物对，其中至少一个引物被设计为掺入5'-特异性序列以提供分子内折叠成二级结构的扩增产物; 并通过包括以下方法检测扩增产物：提供寡核苷酸切割组分，将寡核苷酸切割组分与扩增产物杂交以形成三链切割结构，其中三股裂解结构的两条链由扩增产物，使用产生切割产物的双链体特异性核酸酶活性切割三链切割结构的3'-或5'链，并检测指示靶核酸存在的切割产物。在某些方面，两个引物都包含5'特异性序列，检测包括使用双链体特异性核酸酶切割三链切割结构的3'或5'链以提供切割产物。

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