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1. 发明申请

US20110014617A1 Methods and Systems for Detecting Nucleic Acids 审中-公开
标题翻译：检测核酸的方法和系统
公开(公告)号：US20110014617A1
公开(公告)日：2011-01-20
申请号：US12816006
申请日：2010-06-15
申请人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
发明人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。这些捕获探针比完整的杂交探针更容易与探针片段结合。然后可以在支撑表面上检测标签。以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

2. 发明申请

US20080241838A1 METHODS AND SYSTEMS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：检测核酸的方法和系统
公开(公告)号：US20080241838A1
公开(公告)日：2008-10-02
申请号：US11965837
申请日：2007-12-28
申请人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
发明人： Kristian Scaboo , Vissarion Aivazachvili , Timothy Liu , Robert Eason , Konrad Faulstich
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。这些捕获探针比完整的杂交探针更容易与探针片段结合。然后可以在支撑表面上检测标签。以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

3. 发明申请

US20070099211A1 Detection of nucleic acid amplification 审中-公开
标题翻译：检测核酸扩增
公开(公告)号：US20070099211A1
公开(公告)日：2007-05-03
申请号：US11488439
申请日：2006-07-17
申请人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
发明人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6823 , B01L3/5027 , B01L7/525 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/1844 , B01L2300/185 , B01L2300/1872 , B01L2300/1894 , B01L2400/0418 , C12Q1/6825 , C12Q2565/607 , C12Q2563/131 , C12Q2565/629 , C12Q2531/113 , C12Q2521/301 , C12Q2523/107
摘要： Methods for detecting a target polynucleotide sequences are provided that utilize a probe having a target-complementary segment and a detectable tag. By cleaving the detectable tab and associating the tag with a tag complement coupled to an electrode, an electrochemical signal can be detected that is related to the presence of the tag:tag complement complex.
摘要翻译：提供了检测靶多核苷酸序列的方法，其利用具有靶互补区段和可检测标签的探针。通过切割可检测的标签并将标签与耦合到电极的标签补体相关联，可以检测到与标签：标签补体复合物的存在相关的电化学信号。

4. 发明申请

US20080193940A1 SYSTEMS AND METHODS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的系统和方法
公开(公告)号：US20080193940A1
公开(公告)日：2008-08-14
申请号：US11965807
申请日：2007-12-28
申请人： Vissarion Aivazachvili , Kristian Scaboo , Eugene Spier
发明人： Vissarion Aivazachvili , Kristian Scaboo , Eugene Spier
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2565/607 , C12Q2565/519 , C12Q2521/301 , C12Q2561/109
摘要： A method and kit for detecting a target nucleic acid in a sample is described. The sample to be analyzed may include a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising exonuclease activity that can cleave the hybridized hybridization probe to thereby generate a labeled probe fragment. At least one portion of the hybridization probe hybridizes to another portion of the hybridization probe to thereby form a folded structure. The method can involve melting the sample, reducing the temperature of the sample to allow primer and probe to each hybridize to at least a portion of single stranded target nucleic acid in the sample, elongating the primer and releasing the labeled probe fragment. The sample can be contacted with a solid support comprising surface bound capture probes which hybridize to the labeled probe fragments. The label can then be detected.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。要分析的样品可以包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测标记的第二区，扩增杂交引物的聚合酶和包含外切核酸酶活性的酶，其可以切割杂交的杂交探针，从而产生标记的探针片段。至少一部分杂交探针与杂交探针的另一部分杂交，从而形成折叠结构。该方法可以包括熔化样品，降低样品的温度以使引物和探针各自与样品中的至少一部分单链靶核酸杂交，延长引物并释放标记的探针片段。样品可以与包含与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。然后可以检测标签。

5. 发明申请

US20070175768A1 MICROFLUIDIC SYSTEMS INCLUDING POROUS POLYMER ELECTRODES 审中-公开
标题翻译：包含多孔聚合物电极的微流体系统
公开(公告)号：US20070175768A1
公开(公告)日：2007-08-02
申请号：US11558343
申请日：2006-11-09
申请人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
发明人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
IPC分类号： C12Q1/00 , G01N27/26 , G01N33/487 , G01F1/64
CPC分类号： G01N27/44704 , B01L3/5023 , B01L3/502707 , B01L3/502753 , B01L7/52 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2400/0418 , B01L2400/0421
摘要： Microfluidic devices that incorporate a porous polymer electrode assemblies, including microfluidic device useful for detection of nucleic acids, as well as methods of using the microfluidic devices.
摘要翻译：包含多孔聚合物电极组件的微流体装置，包括可用于检测核酸的微流体装置，以及使用微流体装置的方法。

6. 发明申请

US20070114128A1 Porous polymer electrodes 审中-公开
标题翻译：多孔聚合物电极
公开(公告)号：US20070114128A1
公开(公告)日：2007-05-24
申请号：US11479175
申请日：2006-06-30
申请人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
发明人： Aldrich Lau , Konrad Faulstich , Kristian Scaboo
IPC分类号： C25C7/02 , C25B11/03
CPC分类号： G01N27/3335
摘要： Porous polymer electrode assemblies are useful in the detection or quantification of a variety of analytes. By preparing a porous monolith, and applying a conductive polymer to the monolith, a porous matrix is prepared that combines favorable conductive properties, by virtue of the presence of the conductive polymer, with the porous character of the underlying monolith. The resulting porous electrode can be used for qualitative or quantitative analysis, and the capture and/or release of selected charged materials, such as nucleic acids. The pores of the electrode matrix may also be filled with nonconductive material, yielding electrodes having a plurality of discrete conductive surfaces.
摘要翻译：多孔聚合物电极组件可用于检测或定量各种分析物。通过制备多孔整料并将导电聚合物施加到整料上，制备出多孔基质，其通过导电聚合物的存在与下面的整料的多孔特征结合有利的导电性能。所得的多孔电极可用于定性或定量分析，以及所选择的带电材料（例如核酸）的捕获和/或释放。电极基体的孔也可以用非导电材料填充，产生具有多个离散导电表面的电极。

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