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    • 2. 发明授权
    • Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
    • 制备2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的方法
    • US08759030B2
    • 2014-06-24
    • US10523920
    • 2003-07-07
    • Kazuhisa MukaiKeiji TsusakiMichio KubotaShigeharu FukudaToshio Miyake
    • Kazuhisa MukaiKeiji TsusakiMichio KubotaShigeharu FukudaToshio Miyake
    • C12P19/44
    • C12P19/60
    • The object of the present invention is to provide a method and a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid where 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid comprising the steps of allowing α-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an α-glucosyl saccharide to form 2-O-α-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-α-glucopyranosyl-L-ascorbic acid.
    • 本发明的目的是提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法和方法,其中5-O-α-吡喃葡萄糖基-L-抗坏血酸和6-O-α-吡喃葡萄糖基 - L-抗坏血酸不是以这样的少量形成或形成,也可以不形成。 本发明通过提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法来解决上述目的,其包括以下步骤:将α-异麦芽糖基葡萄糖形成酶与或不与环糊精葡聚糖转移酶(EC 2.4.1.19)一起 作用于包含L-抗坏血酸和α-葡萄糖基糖的溶液中以形成2-O-α-吡喃葡萄糖基-L-抗坏血酸并收集形成的2-O-α-吡喃葡萄糖基-L-抗坏血酸。
    • 6. 发明申请
    • Lactosucrose High Content Saccharide, Its Preparation and Uses
    • 乳糖蔗糖高含量糖,其制备和用途
    • US20080027027A1
    • 2008-01-31
    • US11587248
    • 2005-04-20
    • Hiroyuki OkabeHajime AgaMichio KubotaToshio Miyake
    • Hiroyuki OkabeHajime AgaMichio KubotaToshio Miyake
    • A61K31/702A23L1/236A61K8/72A61P1/00A61Q99/00C12P19/00C13D3/16C13F1/02C13F3/00
    • C13B30/02A23L27/33C07H3/06C12P19/14C12P19/18C13B20/142
    • An object of the present invention is to provide a lactosucrose high content saccharide which comprises 70% or more of lactosucrose, on a saccharide composition basis, with a lower content of 1-kestose and fructosyl lactosucrose as by-products. Another object of the present invention is to provide processes for producing a lactosucrose high content saccharide and high purity lactosucrose containing 90% or more of lactosucrose, on a saccharide composition basis, which are feasible for industrial production. Further object of the present invention is to provide a solid lactosucrose with low hygroscopicity and a solid composition comprising the same. The present invention solves above objects by providing a lactosucrose high content saccharide comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose, on a saccharide composition basis; a process for producing the lactosucrose high content saccharide, comprising the steps of allowing β-fructofuranosidase, derived from a microorganism belonging to the genus Bacillus, and sucrose-unassimilable yeast to contact with an aqueous solution containing sucrose and lactose to obtain a reaction mixture comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose and collecting the resulting lactosucrose high content saccharide; a process for producing a high purity lactosucrose, comprising the steps of subjecting the lactosucrose high content saccharide to a chromatography using a resin and collecting fractions containing 90% or more of lactosucrose, on a saccharide composition basis; a process for producing crystalline lactosucrose; and various solid compositions prepared by incorporating the crystalline lactosucrose.
    • 本发明的目的是提供一种乳糖蔗糖高含量糖,其以糖组成为基础含有70%以上的乳糖蔗糖,其中1-kestose和果糖基乳糖为副产物含量较低。 本发明的另一个目的是提供一种以糖组成为基础制备含有90%以上乳蔗糖的乳糖蔗糖高含量糖和高纯度乳糖蔗糖的方法,其可用于工业生产。 本发明的另一个目的是提供具有低吸湿性的固体乳糖蔗糖和包含它的固体组合物。 本发明通过以糖组成为基础,提供含有70%以上的乳糖和低于1-kestose和果糖乳糖蔗糖总量的3%的乳糖蔗糖高含量糖, 一种生产乳糖蔗糖高含量糖的方法,包括以下步骤:将衍生自属于芽孢杆菌属的微生物的β-呋喃果糖苷酶和蔗糖不可同化的酵母与含有蔗糖和乳糖的水溶液接触,得到包含 70%以上的乳蔗糖,少于1-kestose和果糖乳糖蔗糖总量的3%,并收集得到的乳糖蔗糖高含量糖; 一种生产高纯度乳糖蔗糖的方法,包括以糖组成为基础,使用树脂对乳糖蔗糖高含量糖进行色谱分离并收集含有90%以上乳蔗糖的级分的步骤; 一种生产结晶乳糖的方法; 和通过掺入结晶乳蔗糖制备的各种固体组合物。
    • 8. 发明申请
    • Method for transferring a glucosyl residue
    • 转移葡糖基残基的方法
    • US20070154996A1
    • 2007-07-05
    • US10587711
    • 2005-01-27
    • Tomoyuki NishimotoMichio KubotaShigeharu FukudaToshio Miyake
    • Tomoyuki NishimotoMichio KubotaShigeharu FukudaToshio Miyake
    • C12P19/04
    • C12P19/18C12P19/44
    • An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and/or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and/or a salt thereof; and/or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.
    • 本发明的目的是提供通过酶促反应形成C-6羟基与糖结合的葡萄糖转移的多元醇,葡糖基转移的葡萄糖醛酸和葡萄糖转移的葡萄糖转移衍生物的新方法。 本发明通过提供一种将葡糖基残基转移到多糖,葡糖醛酸和/或其C-6羟基与糖结合的葡萄糖的衍生物的方法,包括以下步骤:使海藻糖磷酸化酶作用于 含有葡萄糖作为组分糖和一种或多种选自肌醇,核糖醇,赤藓糖醇和甘油的多元醇的糖; 葡萄糖醛酸和/或其盐; 和/或一种或多种C-6羟基结合于选自异麦芽糖,龙胆糖,蜜二糖,异麦芽三糖和异丙糖的糖的葡萄糖衍生物。