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1. 发明申请

US20080003651A1 alpha-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
标题翻译： α-异麦芽糖基葡萄糖形成酶，其工艺和用途
公开(公告)号：US20080003651A1
公开(公告)日：2008-01-03
申请号：US11797932
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 并且通过建立形成葡糖聚合度至少为3并且在非还原末端具有α-1,6糖苷键作为连接的α糖异构体糖结合酶和α - 通过催化从葡萄糖聚合度至少为2并具有α-1,4糖苷键作为连接的糖的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦芽糖基转移法; 形成α-异麦芽糖基糖的方法; 制备具有环{66）-alpha-D-吡喃葡萄糖基 - （163）-al-D-glucopyranosyl-（166）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl- （16）同时使用α-异麦芽糖基糖产生酶和α-异麦芽糖基转移酶;以及可用其获得的糖的用途。

2. 发明授权

US07811797B2 α-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
公开(公告)号：US07811797B2
公开(公告)日：2010-10-12
申请号：US11797969
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P1/00 , C12P19/04 , C12N9/00 , C12N9/24 , C07H21/04
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.

3. 发明申请

US20080032350A1 Alpha-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
标题翻译： α-异麦芽糖基葡萄糖形成酶，其工艺和用途相同
公开(公告)号：US20080032350A1
公开(公告)日：2008-02-07
申请号：US11797969
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 并且通过建立形成葡糖聚合度至少为3并且在非还原末端具有α-1,6糖苷键作为连接的α糖异构体糖结合酶和α - 通过催化从葡萄糖聚合度至少为2并具有α-1,4糖苷键作为连接的糖的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦芽糖基转移法; 形成α-异麦芽糖基糖的方法; 制备具有环{66）-alpha-D-吡喃葡萄糖基 - （163）-al-D-glucopyranosyl-（166）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl-（163）-al-D-glucopyranosyl- （16）同时使用α-异麦芽糖基葡萄糖形成酶和α-异麦芽糖基转移酶;以及可用其获得的糖的用途。

4. 发明授权

US07241606B2 α-Isomaltosylglucosaccharide synthase, process for producing the same and use thereof 有权
标题翻译： α-异麦芽糖基糖合酶，其制备方法和用途
公开(公告)号：US07241606B2
公开(公告)日：2007-07-10
申请号：US10089549
申请日：2001-07-25
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 并且通过建立形成葡糖聚合度至少为3并且在非还原末端具有α-1,6糖苷键作为连接的α糖异构体糖结合酶和α - 通过催化从葡萄糖聚合度至少为2并具有α-1,4糖苷键作为连接的糖的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦芽糖基转移法; 形成α-异麦芽糖基糖的方法; 具有环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl-（1-> 6）-al-D-glucopyranosyl-（1）的结构的环四糖的制备方法 - > 3）-al-D-吡喃葡萄糖基 - （1->}，使用α-异麦芽糖基糖形成酶和α-异麦芽糖基转移酶;以及可用其获得的糖的用途。

5. 发明授权

US07718404B2 α-isomaltosylglucosaccharide-forming enzyme, process and uses of the same 有权
标题翻译： α-异麦芽糖基葡萄糖形成酶，其工艺和用途
公开(公告)号：US07718404B2
公开(公告)日：2010-05-18
申请号：US11797932
申请日：2007-05-09
申请人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Keiji Tsusaki , Takanobu Higashiyama , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12P19/04 , C12N9/10 , C12N1/00 , C12N1/20 , C07H21/04 , C07H1/00
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{66)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(166)-α-D-glucopyranosyl-(163)-α-D-glucopyranosyl-(16} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译：本发明的目的是提供一种α-异麦芽糖基葡萄糖形成酶，其工艺，环四糖和包含可通过使用酶获得的糖的糖组合物; 通过建立形成糖类的α-异麦芽糖基葡萄糖形成酶，其葡萄糖聚合度为至少3，并且在非还原末端具有作为键的α-1,6糖苷键和α- 通过催化葡萄糖聚合度至少为2的糖并且具有α-1,4糖苷键作为连接的α-葡糖基转移而在非还原性末端的连接以外的1,4-葡糖苷键所述非还原端基本上不增加所述还原能力; 使用该酶的α-异麦角硫基转移法; 形成α-异麦芽糖基糖的方法; 制备具有环{66）-α-D-吡喃葡萄糖基 - （163）-α-D-吡喃葡萄糖基 - （166）-α-D-吡喃葡萄糖基 - （163）-α-D-吡喃葡萄糖基 - （163）-α-D-吡喃葡萄糖基 - （16）同时使用α-异麦芽糖基葡萄糖形成酶和α-异麦芽糖基转移酶;以及可得到的糖的用途。

6. 发明授权

US08759030B2 Process for producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid 有权
标题翻译：制备2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的方法
公开(公告)号：US08759030B2
公开(公告)日：2014-06-24
申请号：US10523920
申请日：2003-07-07
申请人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44
CPC分类号： C12P19/60
摘要： The object of the present invention is to provide a method and a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid where 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid comprising the steps of allowing α-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an α-glucosyl saccharide to form 2-O-α-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-α-glucopyranosyl-L-ascorbic acid.
摘要翻译：本发明的目的是提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法和方法，其中5-O-α-吡喃葡萄糖基-L-抗坏血酸和6-O-α-吡喃葡萄糖基 - L-抗坏血酸不是以这样的少量形成或形成，也可以不形成。本发明通过提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法来解决上述目的，其包括以下步骤：将α-异麦芽糖基葡萄糖形成酶与或不与环糊精葡聚糖转移酶（EC 2.4.1.19）一起作用于包含L-抗坏血酸和α-葡萄糖基糖的溶液中以形成2-O-α-吡喃葡萄糖基-L-抗坏血酸并收集形成的2-O-α-吡喃葡萄糖基-L-抗坏血酸。

7. 发明申请

US20060216792A1 Process for producing 2-0-alpha-d-glucopyranosyl-l-ascorbic acid 有权
标题翻译：制备2-0-α-d-吡喃葡萄糖基-1-抗坏血酸的方法
公开(公告)号：US20060216792A1
公开(公告)日：2006-09-28
申请号：US10523920
申请日：2003-07-07
申请人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Keiji Tsusaki , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44
CPC分类号： C12P19/60
摘要： The object of the present invention is to provide a method and a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid where 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid are not formed or formed in such a small amount that the formation of these can nor be detected. The present invention solves the above object by providing a process for producing 2-O-α-glucopyranosyl-L-ascorbic acid comprising the steps of allowing α-isomaltosyl glucosaccharide-forming enzyme together with or without cyclomaltodextrin glucanotransferase (EC 2.4.1.19) to act on a solution comprising L-ascorbic acid and, an α-glucosyl saccharide to form 2-O-α-glucopyranosyl-L-ascorbic acid and collecting the formed 2-O-α-glucopyranosyl-L-ascorbic acid.
摘要翻译：本发明的目的是提供一种生产2-O-α-glucopyranosyl-L-抗坏血酸的方法和方法，其中5-O-α-吡喃葡萄糖基-L-抗坏血酸和6-O-α-吡喃葡萄糖基-L- L-抗坏血酸不是以这样的少量形成或形成，也可以不形成。本发明通过提供一种生产2-O-α-吡喃葡萄糖基-L-抗坏血酸的方法来解决上述目的，其包括以下步骤：将α-异麦芽糖基糖糖形成酶与或不与环糊精葡聚糖转移酶（EC 2.4.1.19）一起使用作用于包含L-抗坏血酸和α-葡糖基糖的溶液以形成2-O-α-吡喃葡萄糖基-L-抗坏血酸并收集形成的2-O-α-吡喃葡萄糖基-L-抗坏血酸。

8. 发明授权

US07709230B2 Process for producing isomaltose and uses thereof 有权
标题翻译：异麦芽糖的制备方法及其用途
公开(公告)号：US07709230B2
公开(公告)日：2010-05-04
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44 , C12P19/18 , C12P19/04 , C12N9/00 , C12N9/10 , C12P21/06 , C07H1/00
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种生产异麦芽糖的新方法及其应用，包括以下步骤：将非还原末端具有至少两个葡萄糖聚合度和至少两个α-1,4糖苷键作为键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基葡糖，其在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原端以外的键，和/或形成环{→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （ 1→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （1→};使与异麦芽糖释放酶形成的糖接触释放异麦芽糖;收集释放的异麦芽糖; 其中。

9. 发明申请

US20080241904A1 PROCESS FOR PRODUCING ISOMALTOSE AND USES THEREOF 有权
标题翻译：生产异构体的方法及其用途
公开(公告)号：US20080241904A1
公开(公告)日：2008-10-02
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1″3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种制备异麦芽糖的新方法及其用途，包括以下步骤：将具有至少两个葡萄糖聚合度的至少两个和非1,4-还原末端的α-1,4糖苷键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基糖，其具有在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原末端之外的键，和/或形成环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl - （1-> 6）-al-D-glucopyranosyl-（1''3）-alpha-D-吡喃葡萄糖基 - （1->};使所形成的糖与异麦芽糖释放酶接触以释放异麦芽糖; 释放的异麦芽糖;及其用途。

10. 发明申请

US20070154996A1 Method for transferring a glucosyl residue 审中-公开
标题翻译：转移葡糖基残基的方法
公开(公告)号：US20070154996A1
公开(公告)日：2007-07-05
申请号：US10587711
申请日：2005-01-27
申请人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04
CPC分类号： C12P19/18 , C12P19/44
摘要： An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and/or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and/or a salt thereof; and/or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.
摘要翻译：本发明的目的是提供通过酶促反应形成C-6羟基与糖结合的葡萄糖转移的多元醇，葡糖基转移的葡萄糖醛酸和葡萄糖转移的葡萄糖转移衍生物的新方法。本发明通过提供一种将葡糖基残基转移到多糖，葡糖醛酸和/或其C-6羟基与糖结合的葡萄糖的衍生物的方法，包括以下步骤：使海藻糖磷酸化酶作用于含有葡萄糖作为组分糖和一种或多种选自肌醇，核糖醇，赤藓糖醇和甘油的多元醇的糖; 葡萄糖醛酸和/或其盐; 和/或一种或多种C-6羟基结合于选自异麦芽糖，龙胆糖，蜜二糖，异麦芽三糖和异丙糖的糖的葡萄糖衍生物。

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