专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06638735B1 Plasmid for gene expression in pichia ciferrii and transformation method using the same 失效
标题翻译：在毕赤酵母中用于基因表达的质粒及使用其的转化方法
公开(公告)号：US06638735B1
公开(公告)日：2003-10-28
申请号：US09674826
申请日：2001-07-17
申请人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
发明人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
IPC分类号： C12P2106
CPC分类号： C07K14/39
摘要： Expression cassettes for transforming Pichia ciferrii and their use are disclosed. The present invention relates to expression cassettes containing Pichia ciferrii ribosomal DNA fragment, CYHr gene resistant to cycloheximide, and a desired gene. Expression cassette further comprising Pichia ciferrii GAPDH promoter gene which allows an increase in the expression of the desired gene also provided. Moreover, the present invention provides a process for producing tetraacetyl phytosphingosine using transformed Pichia ciferrii cells with the expression cassettes in a higher yield.
摘要翻译：公开了用于转化毕赤酵母的表达盒及其用途。本发明涉及含有毕赤酵母核糖体DNA片段，对放线菌酮具有抗性的CYHr基因和所需基因的表达盒。进一步包含允许增加所需基因表达的毕赤酵母GAPDH启动子基因的表达盒也被提供。此外，本发明提供了使用转化的毕赤酵母细胞以更高产率用表达盒生产四乙酰植物鞘氨醇的方法。

2. 发明授权

US5698104A Purification process for hirudin using affinity chromatography 失效
标题翻译：使用亲和色谱法对水蛭素进行纯化
公开(公告)号：US5698104A
公开(公告)日：1997-12-16
申请号：US524311
申请日：1995-09-06
申请人： Sang Ki Rhee , Bong Hyun Chung , Eui Sung Choi , Jung Hoon Sohn , Deok Joong Youn , Myung Kuk Kim , Hae Don Lee
发明人： Sang Ki Rhee , Bong Hyun Chung , Eui Sung Choi , Jung Hoon Sohn , Deok Joong Youn , Myung Kuk Kim , Hae Don Lee
IPC分类号： C07K1/22 , C07K1/14 , C07K14/815 , B01D15/08
CPC分类号： C07K14/815 , Y10S530/855
摘要： Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu.sup.++) is used as a metal ion and a phosphate buffer is used as an eluent.
摘要翻译：本文公开了一种从含有水蛭素和其他物质的溶液中净化水蛭素的方法，其特征在于该方法包括使溶液进行金属离子亲和层析的步骤，其中使用铜离子（Cu ++）作为金属离子和磷酸盐缓冲液用作洗脱液。

3. 发明申请

US20130323785A1 METHOD FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR IN LARGE VOLUME FROM YEAST 有权
标题翻译：从日本大批量生产人类生殖生长因子的方法
公开(公告)号：US20130323785A1
公开(公告)日：2013-12-05
申请号：US13883264
申请日：2011-11-04
申请人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
发明人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
IPC分类号： C07K14/485 , C12R1/645
CPC分类号： C07K14/485 , C12N15/81 , C12R1/645
摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
摘要翻译：本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

4. 发明授权

US09260749B2 Genome walking method for cloning of unknown DNA sequences adjacent to known sequences 有权
标题翻译：用于克隆与已知序列相邻的未知DNA序列的基因组步行方法
公开(公告)号：US09260749B2
公开(公告)日：2016-02-16
申请号：US13345616
申请日：2012-01-06
申请人： Jung Hoon Sohn , Jung Hoon Bae
发明人： Jung Hoon Sohn , Jung Hoon Bae
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2525/186
摘要： A method and a kit for cloning a nucleotide sequence adjacent to a known nucleotide sequence by PCR are disclosed. The method includes 1) preparing a DNA fragment with cohesive ends by cleaving a DNA that contains the known first nucleotide sequence and the second nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3′ end of the DNA fragment with a nucleotide analog to block further elongation of the DNA fragment; 3) linking the cohesive ends of the 3′ end-modified DNA fragment with a linker, adapter or cassette having a cohesive end sequence complementary thereto; and 4) performing PCR using a known first nucleotide sequence-specific primer, and a linker, adapter or cassette sequence-specific primer.
摘要翻译：公开了通过PCR克隆与已知核苷酸序列相邻的核苷酸序列的方法和试剂盒。该方法包括：1）通过使用限制酶切割包含已知第一个核苷酸序列的DNA和与其相邻的第二个核苷酸序列，制备具有粘性末端的DNA片段; 2）用核苷酸类似物修饰DNA片段的3'末端以阻断DNA片段的进一步延伸; 3）将3'末端修饰的DNA片段的粘性末端与具有与其互补的内聚端序列的接头，接头或盒连接; 和4）使用已知的第一核苷酸序列特异性引物和接头，衔接子或盒序列特异性引物进行PCR。

5. 发明授权

US08986956B2 Method for producing human epidermal growth factor in large volume from yeast 有权
标题翻译：从酵母中大量生产人表皮生长因子的方法
公开(公告)号：US08986956B2
公开(公告)日：2015-03-24
申请号：US13883264
申请日：2011-11-04
申请人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
发明人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
IPC分类号： C12P21/04 , C12N1/16 , C12N15/09 , C07H21/04 , C07K14/485 , C12N15/81 , C12R1/645
CPC分类号： C07K14/485 , C12N15/81 , C12R1/645
摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
摘要翻译：本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

6. 发明授权

US09719112B2 Mutant beta-glucosidases having enhanced activity and a method for producing bioethanol using the same 有权
公开(公告)号：US09719112B2
公开(公告)日：2017-08-01
申请号：US14111721
申请日：2012-04-13
申请人： Jung Hoon Sohn , Hyun Jin Kim , Chul Ho Kim , Ji Hun Seomoon , Bong Hyun Sung , Kwang Mook Lim , Mi Jin Kim , Jong Hyun Kim
发明人： Jung Hoon Sohn , Hyun Jin Kim , Chul Ho Kim , Ji Hun Seomoon , Bong Hyun Sung , Kwang Mook Lim , Mi Jin Kim , Jong Hyun Kim
IPC分类号： C12P7/06 , C12P7/08 , C12P7/10 , C12N9/42
CPC分类号： C12P7/06 , C12N9/2445 , C12Y302/01021 , Y02E50/17
摘要： The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式