会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 4. 发明授权
    • Genome walking method for cloning of unknown DNA sequences adjacent to known sequences
    • 用于克隆与已知序列相邻的未知DNA序列的基因组步行方法
    • US09260749B2
    • 2016-02-16
    • US13345616
    • 2012-01-06
    • Jung Hoon SohnJung Hoon Bae
    • Jung Hoon SohnJung Hoon Bae
    • C12Q1/68
    • C12Q1/6855C12Q2525/186
    • A method and a kit for cloning a nucleotide sequence adjacent to a known nucleotide sequence by PCR are disclosed. The method includes 1) preparing a DNA fragment with cohesive ends by cleaving a DNA that contains the known first nucleotide sequence and the second nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3′ end of the DNA fragment with a nucleotide analog to block further elongation of the DNA fragment; 3) linking the cohesive ends of the 3′ end-modified DNA fragment with a linker, adapter or cassette having a cohesive end sequence complementary thereto; and 4) performing PCR using a known first nucleotide sequence-specific primer, and a linker, adapter or cassette sequence-specific primer.
    • 公开了通过PCR克隆与已知核苷酸序列相邻的核苷酸序列的方法和试剂盒。 该方法包括:1)通过使用限制酶切割包含已知第一个核苷酸序列的DNA和与其相邻的第二个核苷酸序列,制备具有粘性末端的DNA片段; 2)用核苷酸类似物修饰DNA片段的3'末端以阻断DNA片段的进一步延伸; 3)将3'末端修饰的DNA片段的粘性末端与具有与其互补的内聚端序列的接头,接头或盒连接; 和4)使用已知的第一核苷酸序列特异性引物和接头,衔接子或盒序列特异性引物进行PCR。