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1. 发明授权

US5698104A Purification process for hirudin using affinity chromatography 失效
标题翻译：使用亲和色谱法对水蛭素进行纯化
公开(公告)号：US5698104A
公开(公告)日：1997-12-16
申请号：US524311
申请日：1995-09-06
申请人： Sang Ki Rhee , Bong Hyun Chung , Eui Sung Choi , Jung Hoon Sohn , Deok Joong Youn , Myung Kuk Kim , Hae Don Lee
发明人： Sang Ki Rhee , Bong Hyun Chung , Eui Sung Choi , Jung Hoon Sohn , Deok Joong Youn , Myung Kuk Kim , Hae Don Lee
IPC分类号： C07K1/22 , C07K1/14 , C07K14/815 , B01D15/08
CPC分类号： C07K14/815 , Y10S530/855
摘要： Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu.sup.++) is used as a metal ion and a phosphate buffer is used as an eluent.
摘要翻译：本文公开了一种从含有水蛭素和其他物质的溶液中净化水蛭素的方法，其特征在于该方法包括使溶液进行金属离子亲和层析的步骤，其中使用铜离子（Cu ++）作为金属离子和磷酸盐缓冲液用作洗脱液。

2. 发明申请

US20080074339A1 BENT FOLDED DIPOLE ANTENNA FOR REDUCING BEAM WIDTH DIFFERENCE 审中-公开
标题翻译： BENT折叠DIPOLE天线减少光束宽度差异
公开(公告)号：US20080074339A1
公开(公告)日：2008-03-27
申请号：US11622847
申请日：2007-01-12
申请人： Seung-Chul Lee , Jae-Sun Jin , Myung-Kuk Kim
发明人： Seung-Chul Lee , Jae-Sun Jin , Myung-Kuk Kim
IPC分类号： H01Q9/26
CPC分类号： H01Q9/26 , H01Q21/24 , H01Q21/26
摘要： The present invention relates to a bent folded dipole antenna for reducing a beam width difference, which can reduce a beam width difference, varying with a frequency band, and generate dual polarization through the use of an antenna structure having a bent folded dipole antenna unit, in which a plurality of bent folded dipole components is connected to each other as a single pattern, and a feeding unit for feeding a signal to the folded dipole antenna unit.Therefore, the present invention is advantageous in that it can reduce a beam width difference varying with a frequency band, simplify the structure of the antenna to reduce the cost thereof, and easily obtain dual polarization characteristics and wide band characteristics by combining a feeding unit for feeding a signal in a dual feeding manner with the bent folded dipole antenna unit implemented as a single pattern. In addition, the present invention is advantageous in that current flowing into the feed point of the feeding unit is induced only in folded dipole components without flowing into another feed point, thus realizing excellent isolation characteristics.
摘要翻译：本发明涉及一种用于减小波束宽度差的弯曲折叠偶极子天线，其可以减小随频带变化的波束宽度差，并且通过使用具有弯曲折叠偶极天线单元的天线结构产生双极化，其中多个弯曲的折叠偶极组件作为单个图案彼此连接，以及用于将信号馈送到折叠偶极子天线单元的馈送单元。因此，本发明的优点在于，可以减小随频带变化的波束宽度差，简化天线的结构以降低其成本，并且通过组合馈送单元可以容易地获得双极化特性和宽带特性以双重馈电方式馈送信号，其中弯曲的折叠偶极子天线单元被实现为单一图案。此外，本发明的优点在于，流入馈送单元的馈电点的电流仅在折叠偶极子部件中被感应而不流入另一馈电点，从而实现优异的隔离特性。

3. 发明授权

US07259005B2 Microorganism having ability to convert sterol into androst-4-ene-3, 17-dione/androsta-1,4-diene-3, 17-dione and preparation method and use thereof 失效
标题翻译：具有将甾醇转化成雄甾-4-烯-3,17-二酮/雄甾-1,4-二烯-3,17-二酮的能力的微生物及其制备方法和用途
公开(公告)号：US07259005B2
公开(公告)日：2007-08-21
申请号：US10477447
申请日：2002-05-10
申请人： Seung-Kwon Noh , Myung-Kuk Kim , Won-Tae Yoon , Kyung-Moon Park , Sang-Ok Park
发明人： Seung-Kwon Noh , Myung-Kuk Kim , Won-Tae Yoon , Kyung-Moon Park , Sang-Ok Park
IPC分类号： C12P33/00 , C12P33/20 , C12P33/16 , C12N1/20
CPC分类号： C12P33/00 , C12N1/20
摘要： Disclosed is a microorganism having an excellent ability to convert sterol into and rost-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD), a method for preparation of the microorganism and use thereof, and more particularly, a mutant strain of Mycobacterium fortuitum ATCC 29472, Mycobacterium fortuitum EUG-119, a method for preparation of the mutant and use thereof in preparing AD and ADD. The mutant of the present invention has an excellent ability to convent sterol into AD and ADD which are steroid hormone precursors, compared with the known microorganisms and accordingly, is very useful for mass production of steroid hormones.
摘要翻译：公开了具有优异的将甾醇转化成罗登-4-烯-3,17-二酮（AD）和雄甾-1,4-二烯-3,17-二酮（ADD）的微生物，其制备方法微生物及其用途，更具体地，是偶发分枝杆菌ATCC 29472，偶发分枝杆菌EUG-119的突变菌株，制备突变体的方法及其在制备AD和ADD中的用途。与已知的微生物相比，本发明的突变体具有优异的修饰甾体成为类固醇激素前体的AD和ADD的能力，因此对于大量生产类固醇激素非常有用。

4. 发明授权

US07241589B2 Method for preparation of androst-4-ene-3,17-dione and androsta-1,4-diene-3,17-dione 失效
标题翻译：雄甾-4-烯-3,17-二酮和雄甾-1,4-二烯-3,17-二酮的制备方法
公开(公告)号：US07241589B2
公开(公告)日：2007-07-10
申请号：US10477448
申请日：2002-05-10
申请人： Seung-Kwon Noh , Myung-Kuk Kim , Won-Tae Yoon , Kyung-Moon Park , Sang-Ok Park
发明人： Seung-Kwon Noh , Myung-Kuk Kim , Won-Tae Yoon , Kyung-Moon Park , Sang-Ok Park
IPC分类号： C12P33/00
CPC分类号： C12P33/16
摘要： The present invention relates to a method for preparation of androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD). More particularly, the present invention relates to a method for preparing AD/ADD, comprising the steps of: (a) heating sterols and emulsifier respectively, to dissolve completely and mixing to prepare a mixture; (b) placing the mixture in a water bath at 70˜90° C. and stirring to obtain emulsified sterols; and (c) adding the emulsified sterols to culture media of microorganism as a component. And the present invention relates to a method for preparing AD/ADD, comprising the addition of cyclodextrin-sterol complexes extracted from milk to culture media of microorganism as a component. The emulsified sterols and cyclodextrin-sterol complexes adopted in the present invention can be easily prepared and be effectively used for obtaining AD/ADD at a high yield.
摘要翻译：雄甾-4-烯-3,17-二酮（AD）和雄甾-1,4-二烯-3,17-二酮（ADD）的制备方法技术领域本发明涉及雄甾-4-烯-3,17-二酮（AD）和雄甾-1,4-二烯-3,17-二酮（ADD）的制备方法。更具体地说，本发明涉及一种制备AD / ADD的方法，包括以下步骤：（a）分别加热固醇和乳化剂以完全溶解并混合以制备混合物; （b）将混合物置于70〜90℃的水浴中并搅拌，得到乳化甾醇; 和（c）将乳化的甾醇加入作为组分的微生物培养基中。本发明涉及AD / ADD的制备方法，其特征在于，将从乳中提取的环糊精 - 甾醇配合物添加到微生物的培养基中作为成分。本发明中采用的乳化甾醇和环糊精 - 固醇配合物可以容易地制备并且有效地用于以高产率获得AD / ADD。

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