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1. 发明授权

US06638735B1 Plasmid for gene expression in pichia ciferrii and transformation method using the same 失效
标题翻译：在毕赤酵母中用于基因表达的质粒及使用其的转化方法
公开(公告)号：US06638735B1
公开(公告)日：2003-10-28
申请号：US09674826
申请日：2001-07-17
申请人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
发明人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
IPC分类号： C12P2106
CPC分类号： C07K14/39
摘要： Expression cassettes for transforming Pichia ciferrii and their use are disclosed. The present invention relates to expression cassettes containing Pichia ciferrii ribosomal DNA fragment, CYHr gene resistant to cycloheximide, and a desired gene. Expression cassette further comprising Pichia ciferrii GAPDH promoter gene which allows an increase in the expression of the desired gene also provided. Moreover, the present invention provides a process for producing tetraacetyl phytosphingosine using transformed Pichia ciferrii cells with the expression cassettes in a higher yield.
摘要翻译：公开了用于转化毕赤酵母的表达盒及其用途。本发明涉及含有毕赤酵母核糖体DNA片段，对放线菌酮具有抗性的CYHr基因和所需基因的表达盒。进一步包含允许增加所需基因表达的毕赤酵母GAPDH启动子基因的表达盒也被提供。此外，本发明提供了使用转化的毕赤酵母细胞以更高产率用表达盒生产四乙酰植物鞘氨醇的方法。

2. 发明授权

US08685671B2 Process for producing recombinant glycoproteins by culturing a Hansenula polymorpha mutant strain 有权
标题翻译：通过培养汉逊酵母多形汉逊酵母突变菌株来生产重组糖蛋白的方法
公开(公告)号：US08685671B2
公开(公告)日：2014-04-01
申请号：US13451223
申请日：2012-04-19
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
IPC分类号： C12P21/00 , C12N15/81 , C12N15/90 , C12N1/19 , C12N9/10
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译：本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖多糖的多形汉逊酵母突变菌株通过破坏鉴定的基因来降低糖基化，并使突变株进行各种遗传操作以合成人型聚糖。

3. 发明授权

US08323926B2 Gene from Hancenula polymorpha capable of controlling unfolded protein response and method for increasing effect of secretion using the same 有权
标题翻译：能够控制未折叠蛋白质反应的Hancenula polymorpha的基因和使用其分泌增加效果的方法
公开(公告)号：US08323926B2
公开(公告)日：2012-12-04
申请号：US12304354
申请日：2007-06-13
申请人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
发明人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
IPC分类号： C12P21/06 , C12N15/00 , C12N1/00 , C07H21/04 , C07K1/00
CPC分类号： C07K14/39 , C12P21/02
摘要： The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
摘要翻译：本发明涉及调节未折叠蛋白质反应（UPR）的新型多形汉逊酵母基因和提高重组蛋白的分泌表达效率的方法，更具体地说，涉及提高分泌表达效率的方法重组蛋白通过鉴定，修饰和优化HpHAC1基因，其编码多形汉逊酵母中UPR机制的关键转录调节因子，以及由该基因介导的调节机制。根据本发明，多形汉逊酵母的分泌表达系统可用于大规模生产用于工业和医疗应用的分泌蛋白质，从而以低成本生产大量的蛋白质。因此，该方法被用于生产用于工业和医疗用途的蛋白质，以减少患者的经济负担，并有助于改善全人类的福利。

4. 发明申请

US20100062485A1 Novel Hansenula Polymorpha Gene Coding for Dolichyl-Phosphate- Mannose Dependent Alpha-1,3 Mannosyltransferase and Process for the Production of Recombinant Glycoproteins With Hansenula Polymorpha Mutant Strain Deficient in 有权
标题翻译：新型汉逊酵母多形核糖基因编码多糖基 - 甘氨酸依赖性α-1,3肌醇转移酶的重组糖蛋白基因编码及多形汉逊酵母突变株的重组糖蛋白的制备方法
公开(公告)号：US20100062485A1
公开(公告)日：2010-03-11
申请号：US12091217
申请日：2006-10-26
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
IPC分类号： C12P21/02 , C12N9/10 , C07H21/04 , C12N15/74 , C12N1/19
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译：本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性的α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖蛋白的多形汉逊酵母突变菌株通过破坏鉴定的基因表现出降低的糖基化，并使突变株进行各种遗传操作以合成人型聚糖。

5. 发明申请

US20120202248A1 NOVEL HANSENULA POLYMORPHA GENE CODING FOR DOLICHYL-PHOSPHATE- MANNOSE DEPENDENT ALPHA-1,3 MANNOSYLTRANSFERASE AND PROCESS FOR THE PRODUCTION OF RECOMBINANT GLYCOPROTEINS WITH HANSENULA POLYMORPHA MUTANT STRAIN DEFICIENT IN THE SAME GENE 有权
标题翻译：新型HANSENULA多聚异戊二烯基因编码多赖氨酸 - 曼尼斯依赖性ALPHA-1,3人造丝素和用于生产与其相同基因中的汉逊酵母多聚突变体缺陷的重组型糖蛋白的方法
公开(公告)号：US20120202248A1
公开(公告)日：2012-08-09
申请号：US13451223
申请日：2012-04-19
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
IPC分类号： C12P21/06
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译：本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖多糖的多形汉逊酵母突变菌株通过破坏鉴定的基因来降低糖基化，并使突变株进行各种遗传操作以合成人型聚糖。

6. 发明授权

US08187858B2 Hansenula polymorpha gene coding for dolichyl-phosphate-mannose dependent alpha-1,3-mannosyltransferase and process for the production of recombinant glycoproteins with Hansenula polymorpha mutant strain deficient in the same gene 有权
标题翻译：编码二酰基 - 磷酸 - 甘露糖依赖性α-1,3-甘露糖基转移酶的汉逊酵母多形汉逊酵母基因以及与相同基因缺失的多形汉逊酵母突变株产生重组糖蛋白的方法
公开(公告)号：US08187858B2
公开(公告)日：2012-05-29
申请号：US12091217
申请日：2006-10-26
申请人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
发明人： Hyun Ah Kang , Jeong Seok Park , Moo Woong Kim , Eun Jung Kim , Hye Yun Moon , Doo Byoung Oh , Joo Hyung Heo , Sang Ki Rhee
IPC分类号： C12N1/19 , C12N9/10 , C12P21/00 , C12Q1/48
CPC分类号： C12N9/1051
摘要： The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
摘要翻译：本发明涉及通过使用汉逊酵母多形汉逊酵母系统遗传操作参与糖基化的酶来生产具有降低的糖基化的人型糖蛋白的方法。具体地说，本发明涉及通过鉴定多形汉逊酵母多糖基 - 磷酸 - 甘露糖依赖性的α-1,3-甘露糖基转移酶基因来产生人型糖蛋白的方法，构建产生糖蛋白的多形汉逊酵母突变菌株通过破坏鉴定的基因表现出降低的糖基化，并使突变株进行各种遗传操作以合成人型聚糖。

7. 发明申请

US20100297694A1 NOVEL GENE FROM HANCENULA POLYMORPHA CAPABLE OF CONTROLLING UNFOLDED PROTEIN RESPONSE AND METHOD FOR INCREASING EFFECT OF SECRETION USING THE SAME 有权
标题翻译：来自可控制不相关蛋白质反应的HANCENULA多聚体的新基因和使用其分泌增加效果的方法
公开(公告)号：US20100297694A1
公开(公告)日：2010-11-25
申请号：US12304354
申请日：2007-06-13
申请人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
发明人： Hyun Ah Kang , Hye-Yun Moon , Doo-Byoung Oh , Sang Ki Rhee
IPC分类号： C12P21/06 , C12N1/19 , C12N15/63 , C07K14/00 , C07H21/04
CPC分类号： C07K14/39 , C12P21/02
摘要： The present invention relates to a novel H. polymorpha gene regulating Unfolded Protein Response (UPR) and a method for improving the efficiency of secretory expression of a recombinant protein using the same, more particularly, a method for improving the efficiency of secretory expression of a recombinant protein by identifying, modifying, and optimizing the HpHAC1 gene, which encodes a key transcription regulatory factor of the UPR mechanism in H. polymorpha, and a regulatory mechanism mediated by the gene. According to the present invention, the secretory expression system of H. polymorpha can be used for the mass-production of secretory proteins for industrial and medical applications, thereby producing a large amount of the proteins at low cost. Accordingly, the method is employed in the production of the proteins for industrial and medical use, so as to reduce economic burden for a patient, and contribute to the improvement in the welfare of all mankind.
摘要翻译：本发明涉及调节未折叠蛋白质反应（UPR）的新型多形汉逊酵母基因和提高重组蛋白的分泌表达效率的方法，更具体地说，涉及提高分泌表达效率的方法重组蛋白通过鉴定，修饰和优化HpHAC1基因，其编码多形汉逊酵母中UPR机制的关键转录调节因子，以及由该基因介导的调节机制。根据本发明，多形汉逊酵母的分泌表达系统可用于大规模生产用于工业和医疗应用的分泌蛋白质，从而以低成本生产大量的蛋白质。因此，该方法被用于生产用于工业和医疗用途的蛋白质，以减少患者的经济负担，并有助于改善全人类的福利。

8. 发明授权

US08003349B2 YLMPO1 gene derived from yarrowia lipolytica and a process for preparing a glycoprotein not being mannosylphosphorylated by using a mutated yarrowia lipolytica in which YLMPO1 gene is disrupted 有权
标题翻译：衍生自解脂耶氏酵母的YLMPO1基因和通过使用其中YLMPO1基因被破坏的突变的解脂耶氏酵母的解脂耶氏酵母制备不被甘露糖基磷酸化的糖蛋白的方法
公开(公告)号：US08003349B2
公开(公告)日：2011-08-23
申请号：US12598887
申请日：2007-11-30
申请人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
发明人： Jeong-Nam Park , Yunkyoung Song , Jeong-Yoon Kim , Doo-Byoung Oh , Hyun Ah Kang
IPC分类号： C12P21/06 , C12N9/12 , C12N1/00 , C07H21/04
CPC分类号： C12N15/815 , C12N9/1081
摘要： The present invention relates to a novel YlMPO1 gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPO1 gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.
摘要翻译：本发明涉及一种在工业酵母解脂耶氏酵母（Yarrowia lipolytica）的甘露糖基磷酸化中起重要作用的新型YlMPO1基因，以及能够通过破坏基因产生不含磷酸甘露糖基的重组糖蛋白的宿主系统的方法。通过根据本发明的YlMPO1基因的破坏来抑制甘露糖基磷酸化，从而实现解脂耶氏酵母的糖基化途径的人源化。

9. 发明申请

US20110287463A1 RECOMBINANT BETA-GALACTOSIDASE DERIVED FROM STREPTOCOCCUS PNEUMONIAE 有权
标题翻译：来自STREPTOCOCCUS PNEUMONIAE的重组β-GALACTOSIDASE
公开(公告)号：US20110287463A1
公开(公告)日：2011-11-24
申请号：US12952116
申请日：2010-11-22
申请人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
发明人： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
IPC分类号： C12Q1/34
CPC分类号： C12Y302/01023 , A61K38/00 , C12N9/2471
摘要： The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
摘要翻译：本发明涉及来自肺炎链球菌的新型β-半乳糖苷酶，表现出酶活性的BgaC蛋白质及其使用方法。该蛋白质可用于糖链的修饰和分析，并用作抗癌剂。

10. 发明授权

US07163805B2 Human serum albumin-TIMP2 fusion protein, a polynucleotide encoding the same and a method of producing the human serum albumin-TIMP2 fusion protein 有权
标题翻译：人血清白蛋白-TMP2融合蛋白，编码其的多核苷酸和产生人血清白蛋白-TMP2融合蛋白的方法
公开(公告)号：US07163805B2
公开(公告)日：2007-01-16
申请号：US10888887
申请日：2004-07-08
申请人： Jeong-Yoon Kim , Min-Young Kim , Eun-Kyu Park , Jae-Young Chang , Hyun Ah Kang
发明人： Jeong-Yoon Kim , Min-Young Kim , Eun-Kyu Park , Jae-Young Chang , Hyun Ah Kang
IPC分类号： C12P21/02 , C12N1/21 , C12N1/19 , C12N15/63 , C12N5/10 , C07K14/765 , C07H21/04
CPC分类号： C07K14/8146 , A61K38/00 , C07K2319/00
摘要： The present invention provides a human serum albumin-TIMP2 fusion protein having the amino acid sequence set forth in SEQ ID NO. 10, a polynucleotide encoding the same and a vector comprising the polynucleotide, a host cell transformed with the vector, a method for producing the human serum albumin-TIMP2 fusion protein and a pharmaceutical composition comprising the human serum albumin-TIMP2 fusion protein. The human serum albumin-TIMP2 fusion protein is stable and retains the activity of TIMP2, thus it can be used as a pharmaceutical composition to treat diseases related to angiogenesis and/or metastasis of cancer cells.
摘要翻译：本发明提供具有SEQ ID NO：1所示的氨基酸序列的人血清白蛋白-TMP2融合蛋白。 10，编码该多核苷酸的多核苷酸，包含多核苷酸的载体，用该载体转化的宿主细胞，生产人血清白蛋白-TMP2融合蛋白的方法和包含人血清白蛋白-TMP2融合蛋白的药物组合物。人血清白蛋白TIMP2融合蛋白是稳定的并保留了TIMP2的活性，因此可用作药物组合物来治疗与癌细胞的血管发生和/或转移有关的疾病。

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