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1. 发明授权

US09550997B2 Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins 有权
标题翻译：筛选大量分泌的蛋白质及其作为重组蛋白质生产的融合伙伴的用途
公开(公告)号：US09550997B2
公开(公告)日：2017-01-24
申请号：US12631449
申请日：2009-12-04
申请人： Jung-Hoon Sohn , Jung-Hoon Bae , Hyun-Jin Kim , Kwang-Mook Lim
发明人： Jung-Hoon Sohn , Jung-Hoon Bae , Hyun-Jin Kim , Kwang-Mook Lim
IPC分类号： C12N15/10 , C12P21/02 , C12N15/62
CPC分类号： C12N15/625 , C07K2319/02 , C12N15/1051 , C12P21/02
摘要： The present invention relates techniques for identifying suitable secretion fusion partner (SFP) for hyper-secretory production of recombinant proteins. The SFPs can be obtained from secretome analyzes. Recombinant proteins are produced in a fusion form with a secretion fusion partner (SFP) and can be separated from the SFP by in vitro protease treatment. SFPs of this invention greatly improve the secretion level of target proteins and peptides which are valuable for bio-pharmaceuticals and the bio-industry.
摘要翻译：本发明涉及用于鉴定用于重组蛋白质的超分泌生产的合适的分泌融合配偶体（SFP）的技术。 SFP可以从分泌物分析中获得。重组蛋白以融合形式与分泌物融合配偶体（SFP）一起产生，并可通过体外蛋白酶处理与SFP分离。本发明的SFP大大提高了对生物制药和生物工业有价值的靶蛋白和肽的分泌水平。

2. 发明授权

US08673822B2 Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom 有权
标题翻译：用于产生重组蛋白和翻译融合伴侣的翻译融合伙伴的快速筛选方法
公开(公告)号：US08673822B2
公开(公告)日：2014-03-18
申请号：US10586045
申请日：2004-12-30
申请人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Eung-Suck Lee , Mi-Kyung Shin
发明人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Eung-Suck Lee , Mi-Kyung Shin
IPC分类号： C40B20/04 , C40B30/06 , C40B50/06
CPC分类号： C12P21/02 , C07K14/535 , C07K14/55 , C07K2319/00 , C07K2319/35 , C07K2319/61 , C12N9/20 , C12N15/1086
摘要： Disclosed are a method for rapid screening suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.
摘要翻译：公开了一种快速筛选能够诱导来自多种遗传来源的常规重组生产方法难以产生的非生产性蛋白质的表达或分泌生产的合适的翻译融合伴侣（TFP）的方法，以及蛋白质分泌诱导使用该方法获得的TFP。

3. 发明申请

US20130323785A1 METHOD FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR IN LARGE VOLUME FROM YEAST 有权
标题翻译：从日本大批量生产人类生殖生长因子的方法
公开(公告)号：US20130323785A1
公开(公告)日：2013-12-05
申请号：US13883264
申请日：2011-11-04
申请人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
发明人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
IPC分类号： C07K14/485 , C12R1/645
CPC分类号： C07K14/485 , C12N15/81 , C12R1/645
摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
摘要翻译：本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

4. 发明申请

US20090181425A1 Library of Translational Fusion Partners for Producing Recombinant Proteins and Translational Fusion Partners Screened Therefrom 有权
标题翻译：用于生产重组蛋白质和翻译融合合作伙伴的翻译融合伙伴图书馆筛选
公开(公告)号：US20090181425A1
公开(公告)日：2009-07-16
申请号：US11914437
申请日：2006-07-13
申请人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Mi-Kyung Shin , Sung-Sook Yoon , Chang-Soo Chun
发明人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Mi-Kyung Shin , Sung-Sook Yoon , Chang-Soo Chun
IPC分类号： C12P21/00 , C40B20/00 , C07K14/00 , C40B40/10 , C40B40/06 , C07H21/00 , C12N15/63 , C40B40/02
CPC分类号： C12P21/02 , C07K14/39 , C12N15/1086 , C12N15/81
摘要： The invention relates to techniques for the rapid screening of suitable translational fusion partners (TFPs) capable of inducing secretory production of recombinant proteins, especially proteins that are difficult to produce using conventional recombinant production methods.
摘要翻译：本发明涉及用于快速筛选能够诱导重组蛋白分泌生产的合适的翻译融合伴侣（TFP）的技术，特别是使用常规重组生产方法难以产生的蛋白质。

5. 发明申请

US20100159465A1 Screening of Abundantly Secreted Proteins and Their Use as Fusion Partners for the Production of Recombinant Proteins 有权
标题翻译：筛选大量分泌蛋白，并将其用作重组蛋白生产的融合伙伴
公开(公告)号：US20100159465A1
公开(公告)日：2010-06-24
申请号：US12631449
申请日：2009-12-04
申请人： JUNG-HOON SOHN , JUNG-HOON BAE , HYUN-JIN KIM , KWANG-MOOK LIM , SEUNG LI KIM
发明人： JUNG-HOON SOHN , JUNG-HOON BAE , HYUN-JIN KIM , KWANG-MOOK LIM , SEUNG LI KIM
IPC分类号： C12Q1/68 , C07K14/00 , C07K14/54 , C07K14/575 , C12N9/96 , C07K14/755 , C07K14/745 , C07K14/765 , C12N15/63 , C12N5/10 , C12N1/21 , C12N1/15 , C12N1/19 , C12P21/00
CPC分类号： C12N15/625 , C07K2319/02 , C12N15/1051 , C12P21/02
摘要： The present invention relates techniques for identifying suitable secretion fusion partner (SFP) for hyper-secretory production of recombinant proteins. The SFPs can be obtained from secretome analyses. Recombinant proteins are produced in a fusion form with a secretion fusion partner (SFP) and can be separated from the SFP by in vitro protease treatment. SFPs of this invention greatly improve the secretion level of target proteins and peptides which are valuable for bio-pharmaceuticals and the bio-industry.
摘要翻译：本发明涉及用于鉴定用于重组蛋白质的超分泌生产的合适的分泌融合配偶体（SFP）的技术。 SFP可以从分泌物分析中获得。重组蛋白以融合形式与分泌物融合配偶体（SFP）一起产生，并可通过体外蛋白酶处理与SFP分离。本发明的SFP大大提高了对生物制药和生物工业有价值的靶蛋白和肽的分泌水平。

6. 发明授权

US09260749B2 Genome walking method for cloning of unknown DNA sequences adjacent to known sequences 有权
标题翻译：用于克隆与已知序列相邻的未知DNA序列的基因组步行方法
公开(公告)号：US09260749B2
公开(公告)日：2016-02-16
申请号：US13345616
申请日：2012-01-06
申请人： Jung Hoon Sohn , Jung Hoon Bae
发明人： Jung Hoon Sohn , Jung Hoon Bae
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2525/186
摘要： A method and a kit for cloning a nucleotide sequence adjacent to a known nucleotide sequence by PCR are disclosed. The method includes 1) preparing a DNA fragment with cohesive ends by cleaving a DNA that contains the known first nucleotide sequence and the second nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3′ end of the DNA fragment with a nucleotide analog to block further elongation of the DNA fragment; 3) linking the cohesive ends of the 3′ end-modified DNA fragment with a linker, adapter or cassette having a cohesive end sequence complementary thereto; and 4) performing PCR using a known first nucleotide sequence-specific primer, and a linker, adapter or cassette sequence-specific primer.
摘要翻译：公开了通过PCR克隆与已知核苷酸序列相邻的核苷酸序列的方法和试剂盒。该方法包括：1）通过使用限制酶切割包含已知第一个核苷酸序列的DNA和与其相邻的第二个核苷酸序列，制备具有粘性末端的DNA片段; 2）用核苷酸类似物修饰DNA片段的3'末端以阻断DNA片段的进一步延伸; 3）将3'末端修饰的DNA片段的粘性末端与具有与其互补的内聚端序列的接头，接头或盒连接; 和4）使用已知的第一核苷酸序列特异性引物和接头，衔接子或盒序列特异性引物进行PCR。

7. 发明授权

US08986956B2 Method for producing human epidermal growth factor in large volume from yeast 有权
标题翻译：从酵母中大量生产人表皮生长因子的方法
公开(公告)号：US08986956B2
公开(公告)日：2015-03-24
申请号：US13883264
申请日：2011-11-04
申请人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
发明人： Jung Hoon Sohn , Jung Hoon Bae , Mi Jin Kim , Hyun Jin Kim , Soon Ho Park , Kwang Mook Lim
IPC分类号： C12P21/04 , C12N1/16 , C12N15/09 , C07H21/04 , C07K14/485 , C12N15/81 , C12R1/645
CPC分类号： C07K14/485 , C12N15/81 , C12R1/645
摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
摘要翻译：本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

8. 发明授权

US08865629B2 Library of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom 有权
标题翻译：用于产生重组蛋白和翻译融合伴侣的翻译融合伙伴库
公开(公告)号：US08865629B2
公开(公告)日：2014-10-21
申请号：US11914437
申请日：2006-07-13
申请人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Mi-Kyung Shin , Sung-Sook Yoon , Chang-Soo Chun
发明人： Jung-Hoon Sohn , Eui-Sung Choi , Jung-Hoon Bae , Mi-Kyung Shin , Sung-Sook Yoon , Chang-Soo Chun
IPC分类号： C40B20/04 , C40B30/06 , C40B50/06 , C12P21/02 , C12N15/81 , C07K14/39 , C12N15/10
CPC分类号： C12P21/02 , C07K14/39 , C12N15/1086 , C12N15/81
摘要： The invention relates to techniques for the rapid screening of suitable translational fusion partners (TFPs) capable of inducing secretory production of recombinant proteins, especially proteins that are difficult to produce using conventional recombinant production methods.
摘要翻译：本发明涉及用于快速筛选能够诱导重组蛋白分泌生产的合适的翻译融合伴侣（TFP）的技术，特别是使用常规重组生产方法难以产生的蛋白质。

9. 发明申请

US20120171727A1 NOVEL GENOME WALKING METHOD FOR CLONING OF UNKNOWN DNA SEQUENCES ADJACENT TO KNOWN SEQUENCE 有权
标题翻译：用于克隆与已知序列相关的未知DNA序列的新基因组方法
公开(公告)号：US20120171727A1
公开(公告)日：2012-07-05
申请号：US13345616
申请日：2012-01-06
申请人： Jung Hoon SOHN , Jung Hoon BAE
发明人： Jung Hoon SOHN , Jung Hoon BAE
IPC分类号： C12P19/34 , C09K3/00
CPC分类号： C12Q1/6855 , C12Q2525/186
摘要： A method and a kit for cloning a nucleotide sequence adjacent to a known nucleotide sequence by PCR are disclosed. The method includes 1) preparing a DNA fragment with cohesive ends by cleaving a DNA that contains the known first nucleotide sequence and the second nucleotide sequence adjacent thereto using a restriction enzyme; 2) modifying the 3′ end of the DNA fragment with a nucleotide analogue to block further elongation of the DNA fragment; 3) linking the cohesive ends of the 3′ end-modified DNA fragment with a linker, adapter or cassette having a cohesive end sequence complementary thereto; and 4) performing PCR using a known first nucleotide sequence-specific primer, and a linker, adapter or cassette sequence-specific primer.
摘要翻译：公开了通过PCR克隆与已知核苷酸序列相邻的核苷酸序列的方法和试剂盒。该方法包括：1）通过使用限制酶切割包含已知第一个核苷酸序列的DNA和与其相邻的第二个核苷酸序列，制备具有粘性末端的DNA片段; 2）用核苷酸类似物修饰DNA片段的3'末端以阻断DNA片段的进一步延伸; 3）将3'末端修饰的DNA片段的粘性末端与具有与其互补的内聚端序列的接头，接头或盒连接; 和4）使用已知的第一核苷酸序列特异性引物和接头，衔接子或盒序列特异性引物进行PCR。

10. 发明授权

US06638735B1 Plasmid for gene expression in pichia ciferrii and transformation method using the same 失效
标题翻译：在毕赤酵母中用于基因表达的质粒及使用其的转化方法
公开(公告)号：US06638735B1
公开(公告)日：2003-10-28
申请号：US09674826
申请日：2001-07-17
申请人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
发明人： Sang Ki Rhee , Jung Hoon Bae , Eui Sung Choi , Jung Hoon Sohn , Hyun Ah Kang , Chang Seo Park
IPC分类号： C12P2106
CPC分类号： C07K14/39
摘要： Expression cassettes for transforming Pichia ciferrii and their use are disclosed. The present invention relates to expression cassettes containing Pichia ciferrii ribosomal DNA fragment, CYHr gene resistant to cycloheximide, and a desired gene. Expression cassette further comprising Pichia ciferrii GAPDH promoter gene which allows an increase in the expression of the desired gene also provided. Moreover, the present invention provides a process for producing tetraacetyl phytosphingosine using transformed Pichia ciferrii cells with the expression cassettes in a higher yield.
摘要翻译：公开了用于转化毕赤酵母的表达盒及其用途。本发明涉及含有毕赤酵母核糖体DNA片段，对放线菌酮具有抗性的CYHr基因和所需基因的表达盒。进一步包含允许增加所需基因表达的毕赤酵母GAPDH启动子基因的表达盒也被提供。此外，本发明提供了使用转化的毕赤酵母细胞以更高产率用表达盒生产四乙酰植物鞘氨醇的方法。

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